HPLC - Normal Phase vs Reverse Phase HPLC - Animated

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Basic info about Normal Phase and Reverse Phase HPLC.
There are two variants in use in HPLC
01. Normal Phase - HPLC
02. Reverse Phase - HPLC
The 2 variants depends on the polarity of the Mobile Phase and the Stationary Phase.
Learn the basic concept of High Pressure Liquid Chromatography
01. Introduction
• HPLC - High Perfermanc...
02. The Mobile Phase
• HPLC - What is the fun...
03. The Stationary Phase
• HPLC - The Stationary ...
04. Normal Phase HPLC vs Reverse Phase HPLC
• HPLC - Normal Phase v...
05. HPLC Isocratic vs Gradient analysis
• HPLC - Isocratic vs Gr...
06. HPLC - UV-VIS detection of analytes
• HPLC - UV / VIS detect...
07. HPLC - How to read a chromatogram?
• HPLC - How to read Chr...
08. What is the difference between HPLC and GC?
• HPLC and GC - what is ...

Пікірлер: 66

  • @marina15554
    @marina155545 жыл бұрын

    very simple yet to the point and clear! thank you!

  • @faridyusuf1841
    @faridyusuf18419 жыл бұрын

    Awesome video! easy to understand. Thanks alot

  • @pablocardella5930
    @pablocardella59308 жыл бұрын

    Excellent animation! Very clear! Thanks!!

  • @claral2533
    @claral25337 жыл бұрын

    Thank you so much! This explains the concept in a very clear and easy to understand way. Keep it up sir!

  • @dhruvmini01
    @dhruvmini015 жыл бұрын

    This was extremely useful! Thank you

  • @32barber89
    @32barber895 жыл бұрын

    Thank you so much for this clearly explained video. Although it would have been much better if the video speed was a bit faster and had a voice recording too! But super easy to understand, thanks AGAIN!!!!!

  • @reececowell6642
    @reececowell66428 жыл бұрын

    Very helpful now i can finish my analytical chemistry assignment :)

  • @KriiZInQ
    @KriiZInQ8 жыл бұрын

    This is just awesome, pls keep on making videos on everything. and it will have success

  • @mustafaaslan5910
    @mustafaaslan59108 жыл бұрын

    Great video thanks a lot !

  • @uyenst
    @uyenst9 жыл бұрын

    Thank you for your very clear explanation.

  • @CamilaRodriguez-hv7ls
    @CamilaRodriguez-hv7ls5 жыл бұрын

    So easy. Thank you!

  • @javeriasaeed2074
    @javeriasaeed20742 жыл бұрын

    Thanks alot for saving my time & providing mh the best explanation possible ...💝

  • @wessammohamed9235
    @wessammohamed92355 жыл бұрын

    Clear explanation... but I prefer it to be with voice.. anyway thanks 😍

  • @agentgunnso
    @agentgunnso3 жыл бұрын

    thank so much! it helps me!

  • @prathz1065
    @prathz10652 жыл бұрын

    Awsome, you saved my time👍👍

  • @pradyumnauchale1526
    @pradyumnauchale15265 жыл бұрын

    Very nice animation sir....well explained

  • @chinna2680
    @chinna26804 жыл бұрын

    Thank you so much sir it's very easy to understand 😊

  • @En_hausse55
    @En_hausse555 жыл бұрын

    Thank you so Much, this video is really helpful

  • @myworldofinstrumentation6523
    @myworldofinstrumentation65233 жыл бұрын

    Simple and useful presentation

  • @Safrout007
    @Safrout0076 жыл бұрын

    thank you very much ^^

  • @miriamhernandezgarcia7238
    @miriamhernandezgarcia72383 жыл бұрын

    Nice video!! I understood

  • @Red00731
    @Red007317 жыл бұрын

    Hey i was wondering wha Programm do u use for your animations ?

  • @PrabuddhaSachan
    @PrabuddhaSachan8 жыл бұрын

    Put some more video on hplc... nice video.. easy to understand...!!!

  • @aisharai4491
    @aisharai44913 жыл бұрын

    superb animation

  • @sabavatmamatha9389
    @sabavatmamatha93893 жыл бұрын

    Thank you for this video

  • @zahrazara4492
    @zahrazara44927 жыл бұрын

    thank you it's clear

  • @Andreijkmuyt
    @Andreijkmuyt5 жыл бұрын

    It is all clear with the polar particles.But does a non-polar stationary phase really retain non-polar molecules because they have affinity? What force causes such affinity then?

  • @The_real_Sumaya
    @The_real_Sumaya3 жыл бұрын

    Well done it is so useful

  • @berraozcan3976
    @berraozcan39764 жыл бұрын

    Saved my life

  • @shaikabdullah..
    @shaikabdullah..3 жыл бұрын

    Well explained sir thank you so much

  • @nakkellasatyanarayana9709
    @nakkellasatyanarayana9709 Жыл бұрын

    Very good explanation, thank u sir

  • @mehmoodhassan5355
    @mehmoodhassan53554 жыл бұрын

    thanks so much keep it up ....

  • @catarinalemos1841
    @catarinalemos18415 жыл бұрын

    One question when u have HPLC -Normal Phase your solvent can be hexane cause this molecule is Non Polar like Mobile Phase isn´t it?

  • @ironlotuses2162
    @ironlotuses21625 жыл бұрын

    Better than some professors I would say

  • @khloudmoh1030
    @khloudmoh10308 жыл бұрын

    Thanks so much..

  • @beatrizfreitas9034
    @beatrizfreitas90346 жыл бұрын

    Is the sample to be analyzed in the normal phase polar or non-polar? And in the reverse phase?

  • @pankajpundir4497
    @pankajpundir44975 жыл бұрын

    easy to understand d concept ...thnku so much ...will u pls upload more videos relates to hplc

  • @user-fb4ee6ph1c
    @user-fb4ee6ph1c2 жыл бұрын

    Thank you very much

  • @dhamot.k1561
    @dhamot.k15615 жыл бұрын

    this is simple as simple

  • @Fl0wright
    @Fl0wright4 жыл бұрын

    Great video. But wish there was audio.

  • @kanapathysima9550
    @kanapathysima95504 жыл бұрын

    Thanks for the clear explanation...

  • @leilasvr7144

    @leilasvr7144

    4 жыл бұрын

    thats dont have voice

  • @ChemistryTipsTricks
    @ChemistryTipsTricks5 жыл бұрын

    Thank you sir

  • @darshandarji8440
    @darshandarji84403 жыл бұрын

    I have one question sir Is that Is Stationary phase and identifying spot both are having same state like polar or nonpolar....?

  • @basantmohamed4610
    @basantmohamed46104 жыл бұрын

    why one of the main use of normal-phase chromatography is for separation of isomers???

  • @gerrytan8164
    @gerrytan81647 жыл бұрын

    Hey everyone, im a little unsure about this. For reverse phase hplc, if the non polar moves slower through the stationary phase, what will be affected?

  • @zahrazara4492

    @zahrazara4492

    7 жыл бұрын

    the speed of polar particles which are getting out will be affected

  • @aposvlah1855

    @aposvlah1855

    7 жыл бұрын

    gerry tan why the polar bind then polar??

  • @zaouiamina5799
    @zaouiamina57993 жыл бұрын

    شكراااا

  • @pharmasolutionpakistan7668
    @pharmasolutionpakistan76682 жыл бұрын

    Good

  • @mobarokkorim7902
    @mobarokkorim79025 жыл бұрын

    good

  • @DeepanMadan
    @DeepanMadan8 жыл бұрын

    simple and effective

  • @elenagouitel6041
    @elenagouitel60417 жыл бұрын

    this is good video yet there is no sound?? Thanks

  • @appleseeds5603
    @appleseeds56036 жыл бұрын

    Bless u

  • @alicksinyangwe1838

    @alicksinyangwe1838

    6 жыл бұрын

    very nice video...thanx alick from zambia

  • @neetudhaker8735
    @neetudhaker87354 жыл бұрын

    Mujhe bhi samjh aa gya

  • @Surajcxscsingh
    @Surajcxscsingh8 жыл бұрын

    sir but i don't understand how there will be attraction between two non polar molecules.

  • @Dealcapon97

    @Dealcapon97

    8 жыл бұрын

    +Surajcxs c.singh Like dissolves in like, they are not interacting based on charge.

  • @easylearning981

    @easylearning981

    8 жыл бұрын

    +Surajcxs c.singh All molecular interactions are fundamentally electrostatic in nature and can be described by some variation of Coulomb's Law. Non-polar molecules can be polarized momentaneously, for example by the repulsion of their own negatively charged electron clouds when they are located nearby to each other, so the oscillating dipoles sense each other and couple. This is a favorable attractive electrostatic interaction known as dispersive interactions. Meanwhile polar molecules in the column are experiencing stronger interactions between them. That is why polar and non-polar compounds tend to repeal each other, as you can see trying to mix some water (polar) and vegetable oil (non-polar) in a glass. :-)

  • @farahzahar7830

    @farahzahar7830

    6 жыл бұрын

    oil(non polar) still will be separated from water(polar).

  • @theoryvan5784
    @theoryvan57842 жыл бұрын

    thankyou muchas gracias danke schön terimakasih

  • @Bripirate
    @Bripirate5 жыл бұрын

    So much to be said for the difference between normal and reversed phase HPLC, but this video doesn't say shit!!

  • @chetangoudr3396
    @chetangoudr33964 жыл бұрын

    K

  • @hasridhara
    @hasridhara6 жыл бұрын

    No voice

  • @kayjay1088
    @kayjay10882 жыл бұрын

    Poor audio quality