HPLC | High performance liquid chromatography

HPLC is also known as high performance liquid chromatography or high pressure liquid chromatography. HPLC is usually a modified column chromatography. In HPLC, the a high pressure pump is attached with with the column. This high pressure pump can generate pressure upto 40Mp. The column is further filled with the adsorbent material that has very small particle size.
The small particle size gives a very large surface area for the sample molecules to interact. As a result the efficiency of separation increases giving high resolution.
Following are the different components of HPLC.
The column
The column is made up of stainless steel and can withstand very high pressure upto 50MP. The length of column can vary from 5 to 25cm and has an internal diameter of 4.5mm.
The stationary phase
The stationary phase is made uo of an adsorbent material that has very small particle size. The particle size is kept uniform to obtain better performance.
The mobile phase
Mixture of different solvents is used as a mobile phase. The solvents used depends on the type of sample molecules to be separated. The mobile phase solvents can be polar or non polar.
The detector
In order to detect the eluite (ie sample coming out of the column) the HPLC column is attached with the detector.

Пікірлер: 286

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    @artemiskar18973 жыл бұрын

    youtube once again helping more than the university instructors

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    @shubh7934

    2 жыл бұрын

    KZread is ❤️

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    @ritutak7997

    2 жыл бұрын

    Yes

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    @shiftyjesusfish

    2 жыл бұрын

    Your absolutely 💯 correct

  • @yogitashirsat5969

    @yogitashirsat5969

    2 жыл бұрын

    Exactly!!

  • @naveenb9515

    @naveenb9515

    2 жыл бұрын

    O yeah

  • @CM-yp2hg
    @CM-yp2hg Жыл бұрын

    Here cuz I lied on my resume

  • @haven3911

    @haven3911

    Ай бұрын

    How’d it go?

  • @CM-yp2hg

    @CM-yp2hg

    Ай бұрын

    @@haven3911 It actually went really well. It’s not that I have no idea what an hplc is but I was not proficient enough to put it down in my resume by any means. I didn’t get that particular job but I applied to a few other and within a couple of weeks I became an analytical chemist and I am very well proficient in it now :)

  • @sinchanakr6156

    @sinchanakr6156

    25 күн бұрын

    Hey can you help me

  • @ArtuursPaulausks
    @ArtuursPaulausks2 жыл бұрын

    Well, as a guy who daily works with HPLC have to say video is very good for brief introduction. Obviously HPLC is more complicated, with much more details. Just want to give shout out to all chemistry students and tell them to not be afraid of HPLC. In most cases, it is easy to develope a method on HPLC to determine some kind of compound, it might take just a few tries to make sure your compound of interest is separated from rest. Just always remember to filter your samples using 0.45 um filters and inject clean samples, also use guard columns :)

  • @TrangNguyen-nw9dq

    @TrangNguyen-nw9dq

    Жыл бұрын

    Dear, It is grateful when I read your comment. Could you suggest some videos which relate to developing method analysis on HPLC. I am a student who is learning HPLC to support for Master's and phD courses. Therefore, I appreciate if you could support me. Thank you so much.

  • @animaluveteranu9523

    @animaluveteranu9523

    Жыл бұрын

    don t forget to mesure you r standards corectly and shake them on ultrasonyc device It happends to my colegue and he finds out after 3 days XDDDD but I have a question for you if you work on hplc are you working on spectofotometers that use uv light?

  • @ArtuursPaulausks

    @ArtuursPaulausks

    Жыл бұрын

    @@animaluveteranu9523 UV/VIS thermo Dionex Ultimte

  • @user-xg5wh5ft4w

    @user-xg5wh5ft4w

    11 ай бұрын

    Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

  • @helmetongrass1893

    @helmetongrass1893

    10 ай бұрын

    hey, uhh.... can you please tell me if he said absorbent or adsorbent material? I mean when he is talking about the material in the column... 1:34 Edit: Nevermind, it's adsorbent material

  • @srenathganesan
    @srenathganesan2 жыл бұрын

    Awesome explanation. No need to search for any other topic. Its a complete combo package. Thank you very much for making such a video.....

  • @nbent4607
    @nbent46072 жыл бұрын

    Real-life heroes don't wear capes, a man once said. Thanks a lot!

  • @wiggywoman5184
    @wiggywoman51842 жыл бұрын

    thank you for this video! i'm in a biochem grad program but coincidentally never took an actual biochem in undergrad, so these videos are helping a lot!

  • @cioccolatamania3622
    @cioccolatamania36223 ай бұрын

    Thanks!! I am truly amazed by chemists and engineers that develop these analytical instruments

  • @rachmadamayanti1858
    @rachmadamayanti18582 жыл бұрын

    Wow, a really great explanation! If this video was launched during my college time six years ago, I would have been a great Chemist who confident about using HPLC.

  • @user-xg5wh5ft4w

    @user-xg5wh5ft4w

    11 ай бұрын

    Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

  • @shinchanshinchan9155
    @shinchanshinchan9155 Жыл бұрын

    Thank you so much sir it's really a clearcut explanation i can't believe this almost a huge explanation is covered in 7mins 36sec

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    @aryanshah19433 жыл бұрын

    perfect explanation, thank you!

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    @anamariatorres69172 жыл бұрын

    The best explanation of HPLC. Thank you so much

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    Explaining amazingly giving maximum concepts

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    @cozmos36787 ай бұрын

    Brief and clear explanation... Thank u❤️

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    @iroshasanjeewani6080 Жыл бұрын

    Absolutely ,This was helped me to undestand about HPLC.Thank you ❤.

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    @jhansilanka18694 жыл бұрын

    Excellent explanation i undertood very easily

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    @sashansachinda98124 жыл бұрын

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    @nithyasahayarubi36663 ай бұрын

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    @daanstrik4293 Жыл бұрын

    Currently working on my internship report and needed a quick refresher. This video has been one of the most time efficient summaries i've found. Cheers!

  • @user-xg5wh5ft4w

    @user-xg5wh5ft4w

    11 ай бұрын

    Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

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    @sabinahasanova7198 Жыл бұрын

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    @roroiyaka2 жыл бұрын

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    @MB-vd6hc4 жыл бұрын

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    @LinitedfiA3 жыл бұрын

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    @NannannanSJN3 жыл бұрын

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    @TechsScience Жыл бұрын

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    @dawixon12 жыл бұрын

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    @alphynisasteaphen2822 жыл бұрын

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    @42najibshaikh982 жыл бұрын

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    @drashtidarji6 ай бұрын

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    @AA-gl1dr2 жыл бұрын

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    Bastante útil para abordagem preliminar sobre HPLC

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    @Ocyon3 жыл бұрын

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    @ahmadfares52432 жыл бұрын

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    @chennamsettiprasad7257 Жыл бұрын

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    @HozanaaАй бұрын

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    @rowidakhalily57604 ай бұрын

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    @MayankSingh-uj6hi4 жыл бұрын

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    @shirinmittal32252 жыл бұрын

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    @esraaalmasoudy46303 жыл бұрын

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    @ahmadhamza66183 жыл бұрын

    Best vedio to understand hplc

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    @Lia_liah2 жыл бұрын

    Thank you for the vedio and by this vedio i understood the topic very clearly

  • @claudiaatsu1056
    @claudiaatsu10562 жыл бұрын

    That is a very good explanation! Thank you, is there any paper that you suggest that explains the function of HLPC? In order to detect vitamin K with the technique of HLPC, is there only that procedure or is a combine procedure with another technique? Thanks

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    @Serwada2374 ай бұрын

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    @samuelbabalola34792 жыл бұрын

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    @scimathlab1973 жыл бұрын

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    @shabinmillet92618 ай бұрын

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    Osmm it's enough for exam . 🔥

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    @iddah37323 жыл бұрын

    Very well explained. I can now answer any question in HPLC with ease.thank you

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    @naveenb9515

    2 жыл бұрын

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    @anchorsreyashi2 жыл бұрын

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    @samueld773 жыл бұрын

    Great video

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    @btsforever51232 жыл бұрын

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    @dilshanchathuranga69625 ай бұрын

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    @gandhamgracy49372 жыл бұрын

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    @kalyanirajalingham12862 жыл бұрын

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    @shaikabdullah..3 жыл бұрын

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    @mdraselmiah34034 жыл бұрын

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    @hafizghaniullahsafi68532 жыл бұрын

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    @abdulrahman-ob5wx6 ай бұрын

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    @EnglishExcel22084 жыл бұрын

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    @casper29173 жыл бұрын

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    @setupatel14766 ай бұрын

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    @professorawais34533 жыл бұрын

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    @RylanVG10 ай бұрын

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    @anthonytan61432 ай бұрын

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    @myworldofinstrumentation65233 жыл бұрын

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    @thewwefan574 жыл бұрын

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    @mortezahadizadeh96372 жыл бұрын

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    @naveenb95152 жыл бұрын

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    @nk_17

    3 жыл бұрын

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