Got the degree because of you❤😁 thank you sir... from sri lanka

@shomusbiologyofficial

Жыл бұрын

All the best

@hirushapeiris4842

Жыл бұрын

@@shomusbiologyofficial ❤💪

You explain this better than any of my university professor's ever could. Thank you.

@faisalali-mz5hs

6 жыл бұрын

Yeah ..... same here

@reinstamedia551

5 жыл бұрын

really

@sreeram6416

3 жыл бұрын

@@faisalali-mz5hs in many universities teachers are lazy

@sudipnale7664

3 жыл бұрын

Same

@MuhammadNadeem-st8pd

3 жыл бұрын

You must be a back bencher lol

I am a MSc microbiology student, I pass my degree bcz of your classes .Now also I used to watch your classes.Thank you very much sir😊

@shomusbiologyofficial

Жыл бұрын

Thank you

@reddylakshmim9022

4 ай бұрын

Hlo sis I want to do msc biotech which clg is best

omg your the best dude, not even my professor explained it this well. Never stop bro, consider yourself a life saver!

The best explaination on HPLC, ever. I have never heard of any explaination better than yours. I mean my professors dont teach us like that. Thank you, sir.

@shomusbiologyofficial

5 жыл бұрын

Glad you liked my lectures. Please subscribe and share

Better come to our university and teach our staffs how teach the students...Really your teaching is good

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

I love this tecnology , my final proyect of university was about ultra performance chromatography liquid- spectromatry mass UPLC -QTOF for detected some specific monomers of cutin in fruits , now I follow your job in KZread I like

This video is HIGHLY informative and extremely clear. I understand HPLC now, more than I ever have. Wow! Thank you for taking the time to help us all. :)

One of the best, if not the best, video for HPLC i've watched. Thank you so much.

I've hard problems understanding this topic. Shomu's made it all simple. I will stick by these lectures. Thanks a lot.

Thank you for the all of your explanation videos..most of our university badgemates watch your videos to understand Biochemistry lessons.Thank you From SriLanka❤

Just 5 days to go for our university sem exam..and thanks to god..We have you here with us😍

Thank you so much for sharing the information! I've been to class and go through the notes given but still I couldn't get in but after I watch this video it really help me to understand the principle of HPLC! Very good explanation and easy to understand!

No one can't replace u sir...great lecture...keep doing, thank u sir.

Comrade, your explanations are very simple, well organised and detailed at the same time. You are one of the best teachers on KZread about chemistry.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

Thank you very much, Suman! You explain very well! Especially the one with the Gas Chromatography lecture. It is very interesting and understandable thanks to you. You know how to teach propely.

Splendid! I didn't need to go through my lecture notes in detail. Thanks

Wow, thank you so much 😭😭you saved my life to think I have an exam in 3 days ❤️❤️ ❤️🙏🙏

@shomusbiologyofficial

4 жыл бұрын

All the best for the exam

i always go back to yr vid sir since study and now even for my job. thank u so much! best online teacher ever!!

@shomusbiologyofficial

Жыл бұрын

You're welcome. Glad you liked it

My favorite teacher, your lesson really helps me a lot. Keep doing so bro. Being a teacher is your precious gift.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

Hi Shomu, i just Watch my first video from you on gas chromatography and it was easy and fun to learn. Thank you.

Thank you so much, Suman & Shomu's Biology, very well explained.

Very nicely and in a very simple language it is explained the complex system.

AoA sir i am really impressed by the way u teach chromatography.its remain very helpful for me and clear my doubts, sir thanks alot

Appreciate your clarity of thoughts . Your efforts to promote science thro lectures like this amazes me . Keep it up and continue your good work .

@shomusbiologyofficial

4 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

briefly, nicely explained #HPLC good speaker thank you sir.....

Thank you so much, sir! It helped me to have insights about HPLC for my (which is supposed to be our) thesis :)

Very nice explained!! I like it so much !! Very nice study I'm proud of sir !!!God bless u !!!

Thanku sir... thanks a lot for this video, it helps me a lot.....keep going and keep shining🎆🎆

Awesome video!! 😆 VERY INFORMATIVE AND STRAIGHT TO THE POINT.........

last step of instrument is very helpful to clear confuseness so again thanks a lot

you are eplaining HPLC chromatography much better than my proffessor at university , tanks for that.

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Good job explaining the basic of HPLC.

not all heroes wear capes.. thank you for your efforts

Very nice presentation .....understand everything in simple path........Thanks alot.....wish you a nice future,,,

clear d concept of HPLC...thank u Sir. ...good explaination...

i love the way you explained.. i nver find it so easy.thanks

Very clear about the topic. The best lectures I attend ever 🙏

@shomusbiologyofficial

3 жыл бұрын

Glad to hear that you're getting benefit from my lectures

systematic and simple approach to topics makes it easy to absorb...thanku

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

Tq u Sr u r clases are very help full to undstand than our lecturers....... Tq u so much sr

You are the best teacher 🧬💕

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

you have told the traditional and the reverse phase chromatography as same...the mobile phase as philic and stationary phase as phobic....while explaining... please do check and tell what exactly the difference is thanq..this video helped me a lot

You can think of the interaction between the sample and the stationary phase as someone stopping to chat with a person they like, which delays them from getting to where they need to be. If you do not like the person, you do not stop to chat and speed past them/avoid eye contact (ei. Hydrophobic molecule zooming by hydrophilic stationary phase.)

@pranabkumarbiswas7285

3 жыл бұрын

Nicely described. Will it be possible to develop and describe a vedio on Glycated haemoglobin ( HbA1c) chromatogram ?

You are explaining complex things in very simple way . I learned a lot thanks a lot..

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

GC is mainly used for volatile and semi-volatile analytes. Biological samples are not ideal for GC-MS not because they are vaporized but they can't be vaporized. Rather, biological samples are thermo-labile and degrade at high temperature in GC column. But, process such as derivatization can enhance volatility and thermo-lability of biological analytes such that you can analyze in GC-MS.

Thanking you alot for saving us from boring lectures of our Professors....you are the saviour of millions of the students like me who have not Studied at all during the whole year becoz we knew that we have our beloved teacher Shomu sir on KZread to teach complicated things just like alphabets. We really appreciate your efforts for making Science easy and lovable.... thnakuhh 😊 @Sagar_University

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts to help

Very good explanation about hplc thank you sir

Waoh its straight and the explaination is so clear. thank you

Hello, thank you for this video! But I am confused as I have seen absorbance on Y-Axis , does the area correlation still works in this case?

wonderful explanation brother u done a great video is very useful for pharma students....

Hi. Do you have any idea about USP L37&L38 phase know as polymethycrylate type phase, which is work 100% water mobile phase, which solvent the packers pack the column.

now i undertand this ! thank you so much

Great refresher, thank you!

You are explaining a very nice n clear n easy to understand..thank you

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

u make the things very clear and easy. thankuuuuuu so much sir

Thank you so much 😊 Sir 🙏. Your video classes are not my first options or last options They are my Only options 🙏 These are Very much helpful. So many good wishes for u Sir🙏.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts. Stay tuned for more

Nice and clear understanding lecture i have ever seen

Another great video, thank you!

What about the back pressure which is linked with HPLC? U didn't covered that part sir

You saved me too many times that i can count. Thank you ❤❤

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

Amazing explanation about HPLC . Very clear concept with basic cover .

@shomusbiologyofficial

Жыл бұрын

Thank you so much for appreciating my efforts

sir may i knw wat does theoritical plates and plate count exactly indicates in a chromatogram?

this is great! Thank you! but... what do you use for elutent?

You're a legend. Thank you!

My university professors don't even explain...you tube is my only professor...thanks to technology and all the professors who are uploading their lectures here...❤

@shomusbiologyofficial

Жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear

Your explanation is really nice. Basic concepts was clears specially hydrophobic and hydrophilic. Thank u so much

@shomusbiologyofficial

5 жыл бұрын

You're welcome

Method of teaching is awesome

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

Amazing video great explanation.

Thanks! I really liked the way you explained it. Plain and simple but very informative at same time.

@shomusbiologyofficial

7 жыл бұрын

+Dinesh Kumar thank you. Glad you liked it

Hi , can u tell specifically which type of sample is used (name) for GC (volatile) & HPLC chromatography?

I understand HPLC because of you. Thank you.

@SafdarHussain-cn3fr

3 жыл бұрын

same with me

Thanks for your awesome video

You are really great man thanks for your efforts

Will this method of chromatography work in separating pesticides from THC oil?

i have to do some HPLC soon. its about HPLC of metabolites in samples. i intend to use ethanol to extract these metabolites. and literature says i have to use acetonitrile and distilled water (40:60) as my mobile phase and c18s as my stationary phase. but i have to reconstitute my control in chloroform, before use. are these comaptible?

Thank you very much .you have explained this technique better than anyone else ☺

@shomusbiologyofficial

5 жыл бұрын

Thank you so much for appreciating my efforts

what kind of detector and column are use in hplc ?

hello my friend .. plz can you tell me about best solvents for C18 coloumn cleaning?

you are really awesome brother i will not able to express my all thank you for you...

@shomusbiologyofficial

6 жыл бұрын

Glad to help you out

Is it need to do the dilution of a sample before undergo the process using hplc?

Excellent way of Explanation

RPHPLC Mai sample b hydrophobic hga tou Kya krenge??

I wish..you were my teacher in my college...

@shomusbiologyofficial

4 жыл бұрын

Now you can learn anytime from my lectures

Good explanation, it is in general, if yiu explain with a protein sample, it is very good to understand what chemicals we can use

it is always wonderful to learn from you. may Allah always bless you Ameen

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Thank you!! Finally I understand that!! 😊

i wish we could get you as a professor for our class :)

Thanks!! What you have mentioned in video is really detailed. And I do need this.

@shomusbiologyofficial

7 жыл бұрын

+sabrina run thank you. Glad you liked my lectures

hplc column packing order (strongest polar to weakest) I unable to find this in book

Quick question: let's say you have a polar stationary phase and a polar mobile phase with a polar analyte. Instead of the stationary phase slowing the movement of the analyte due to polar affects, wouldn't the stationary phase speed the movement of the analyte by "pulling" it through the tube via polar affects? Thanks

such a nice seminar on hplc

Great tutorial. Thanks. Please post a tutorial for how to read chromatogram for quantitative analysis.

very nice presentation, full logic concept on the hplc. thank you

@shomusbiologyofficial

7 жыл бұрын

+Yarro Paschal thank you. Glad it helped

So In reverse phase interaction of polar with non polar is more?

I FINALLY GOT IT! YOU ARE A LIFE SAVER!

@shomusbiologyofficial

Жыл бұрын

Thank you

thank you so much for this, you made mention of the RPHPLC using a liquid as the stationary phase hence it is liquid -liquid chromatography, please which liquid is used as the stationary phase?

Sir ,what are the hydrophobic stationary phase used in hplc?

this was really helpful thanks so much for the video but my teacher is making us study a diagram with a pulse damper, drain valve, filter, back pressure and transducer and i've no idea what those are for lol...

Very very important this chapter

Thanks you sir for making my problems clear.....