Reversed phase liquid chromatography
Overview of the retention mechanism in the reversed-phase HPLC. Read by Anneli Kruve from Stockholm University. Learn more about studying analytical chemistry at Stockholm University: www.su.se/english/search-cour... And about Kruvelab: kruvelab.com/
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What a great explanation! Clear and concise!
Omg this is amazingly well-explained. Thanks!
Thank you very much for your wonderful lecture.
this is a great video. clearly explained
Very helpful thank you:)
Just awesome
I think you made me love cheistry again
You NAILED it!! Best Video on Mobile phases. Only request is voice in the video is distorted/noisy, Pl fix it for us. Thanks for making a informative video
thank you
Likedddd it
This is amazing!! Can you tell me in which source I can find the images?
In effect of pH case at pH=5.0 there is almost equal concentration of benzoic acid and benzoic ion so why we didn't getting two peaks Plzzzzz ans
@anneliscienceblog2562
2 жыл бұрын
Very good question! The reason is that the equilibrium between neutral form and anion is very-very fast in comparison to separation time. So each benzoic acid molecule will be about half of the time as neutral molecule and about half of the time as an anion. When the molecule is in the neutral form it will move slower but when it is anion it will move faster through the column. Therefore, it will show a retention behavior that is an average of the behavior of purely neutral form and purely anionic form.
Okay but not understand because don't know English completely