Reversed phase liquid chromatography

Overview of the retention mechanism in the reversed-phase HPLC. Read by Anneli Kruve from Stockholm University. Learn more about studying analytical chemistry at Stockholm University: www.su.se/english/search-cour... And about Kruvelab: kruvelab.com/

Пікірлер: 14

  • @aohemeng
    @aohemeng2 жыл бұрын

    What a great explanation! Clear and concise!

  • @MrTjStylez
    @MrTjStylez3 жыл бұрын

    Omg this is amazingly well-explained. Thanks!

  • @SG73088
    @SG730883 жыл бұрын

    Thank you very much for your wonderful lecture.

  • @mahima9251
    @mahima92512 жыл бұрын

    this is a great video. clearly explained

  • @Gracebarm
    @Gracebarm3 жыл бұрын

    Very helpful thank you:)

  • @shazalatunbintehudaepi1446
    @shazalatunbintehudaepi14463 жыл бұрын

    Just awesome

  • @studymantra3720
    @studymantra372010 ай бұрын

    I think you made me love cheistry again

  • @sandeeppallerla5902
    @sandeeppallerla59023 жыл бұрын

    You NAILED it!! Best Video on Mobile phases. Only request is voice in the video is distorted/noisy, Pl fix it for us. Thanks for making a informative video

  • @alhaeri1
    @alhaeri1 Жыл бұрын

    thank you

  • @jessefebrina2325
    @jessefebrina2325 Жыл бұрын

    Likedddd it

  • @anoukvanderveen5100
    @anoukvanderveen5100 Жыл бұрын

    This is amazing!! Can you tell me in which source I can find the images?

  • @AliRaza-wp8tr
    @AliRaza-wp8tr2 жыл бұрын

    In effect of pH case at pH=5.0 there is almost equal concentration of benzoic acid and benzoic ion so why we didn't getting two peaks Plzzzzz ans

  • @anneliscienceblog2562

    @anneliscienceblog2562

    2 жыл бұрын

    Very good question! The reason is that the equilibrium between neutral form and anion is very-very fast in comparison to separation time. So each benzoic acid molecule will be about half of the time as neutral molecule and about half of the time as an anion. When the molecule is in the neutral form it will move slower but when it is anion it will move faster through the column. Therefore, it will show a retention behavior that is an average of the behavior of purely neutral form and purely anionic form.

  • @fuemah8313
    @fuemah831310 ай бұрын

    Okay but not understand because don't know English completely