OMG I really love all of these videos 'cause it's helping me a lot for tuesday's final exam

For those struggling with calculating the concentration for compound X; notice the ratio difference between 200mg/l and 250mg/l, the pattern is that for 1 mg, the peak area increases with 1000. Therefore its only a matter of multiplying peak area for compound X with 1mg/1000... The answer is as stated in the video: 207,757 mg/l

@user-cj8zj8sc2g

5 жыл бұрын

So you divide by 1000 in order to get the concentration but the Peak area is = to 200,000? So how has the answer become 207,757?

@whitealger.9540

5 жыл бұрын

@@user-cj8zj8sc2g No,the peak area is 207757(p.area of compound X). u have every 1mg increases with1000 p.area,so how many mg in 207757p.area? So :207757x1/1000=207,757

Yo dat peak area was THE SHIT, bro! 11/10 would analyze again.

@aposvlah1855

7 жыл бұрын

2357y1113 what is mg/l ?

@nadiaazman6980

7 жыл бұрын

miligram per litre. a concentration unit.

@BraKahan

6 жыл бұрын

THAT IS PPM YOU PEASANT

Lovely.. most easiest explation. Thanks a lot

Your all videos are well explained. I really enjoyed. Keep it up and upload more

Wow! Thank you. I wish you were my science teacher

Excellent explanation! Thanks!

Loved the way of explanation....hats off...keep up the good work

very well explanation. Thank you.

Thank you for the fantastic vid 👍🏽😎😎

Thanks for the video. 👌

thank you very muchh

Thank you for this!

amazing, thank you!

good...very well explained..

I liked to watch more of your videos in other interesting technics

Thank you so much.

Clearly explanation cheers

Very good job sir ji Thank you so much

It was so helpful, thanks a lot:)))

Superb!!

thank you!!!

this saves my assignment >< thanks a lot

Thank you for this video. It is helpful.

Great job

tqsm for this well explained video. it helps me a lot

well , super ,tq so much

Explanation is good But it’s so irritating to watch video without voice

really amazing

Thank you this is what I want

अति श्रेष्ठ व्याख्यान धन्यवाद

this is good and informative video

Love It .♥

Thank's :)

simplicity is the ultimate sophistication

Nice elaboration.

LOVE U!!!

By far the best video I’ve ever seen for explaining this in simple terms, I now get it, Thank you ❤

Thank you for saving my grades at Uni

@niftypubgmobile2261

5 жыл бұрын

Good luck at uni

This was very helpful to me. I have a question for you. I need to create a tool (either in R, Excel or python) to calculate and plot the critical peak pair (pair with the lowest resolution) for a given set of parameters. Firstly, what is "resolution" in this context? Also, can you point me to literature that would help me come up with this tool?

super ji I am watching this series of video which is more informative than my book I am preparing for analytical engineering test using this video

Excellent

Very helpful

Hi Sir, In your video, you stated that the peak area represents the amount of compound that has passed the detector. Does this statement apply to the chromatogram with mV as y-axis as well? Looking fwd to your reply. Thanks

easy to understand

👍🏻

Thanks but it will be great with voice

What else does the peak height indicate if it doesn't guarantee more concentration than any other below jit

What does it mean if the baseline is not straight? Is it an error with sample prep, or an error with detector?

@helix7564

8 жыл бұрын

+Jebus Slaves try to adjust pH, or other columns. It could be many reasons. The chemicals react with the filling materials, or two components are not separated thoroughly, or it could be isomers, or try to adjust the gradient time of mobile phase.

@MrFeelxd

7 жыл бұрын

In my case, I had also troubles in not staight baselines, and the reason was preassure, a strong hydrophobic compound in Reversed phase HPLC can be stuck at the begining of the column, one way to solve it is turning around the column. By doing this, they can be eluted easily.

I uploaded practical video on HPLC and HPTLC analysis using software OpenLab and Visioncats...

I did my master's degree in analytical chemistry but synthetic organic chemistry for PhD. how to determine syn:anti from hplc spetra?

@aposvlah1855

7 жыл бұрын

chem chem so what is mg/l ? how is this concetration? is this ppm?? why the peek 277456 pf compound x is 277,456 mg/l ??

what does it mean that a column has been calibrated?

Where do you get the retention times from?

@sonison668

4 жыл бұрын

as in the reference list of compounds and retention times

kindly help me, what is the exact formula to calculate syn:anti from hplc spetra? this video explanation not clear to me. from retention time, area, peak and concentration values, how to determine syn:anti of chiral compound?

@taotao2623

5 жыл бұрын

I think chiral compounds also have different R-time, maybe you can find the explaination from instrumenal analysis

So how do you calculate the concentration of the unknown sample?

@alexsenior9385

4 жыл бұрын

Divide the area of unknown by area of known then multiply by the concentration of known. Hey presto!

@cristhianforero2731

Жыл бұрын

Y=mx+b

Can you please provide raw hplc data file so that , can learn to interpret them

Geaaaaaaaaaaaaaaaaaat

1:21 why all samples are named C1?

@TheIHaZZarD

5 жыл бұрын

A bit late but it's because it's the same sample (Caffeine) but with an increasing concentration with the purpose of making the calibration curve.

Which phenomenon couldn explain the bad baseline?

@ivanmegafanboy1981

4 жыл бұрын

Maybe the mobile phase absorbs some of the light from the detector, so it is set in a bad wavelenght; or the sample contains a wide mixture of compounds.

does anyone have audio complications?

Duke city

OK

I wish there was talking

not even close to HPLC

I did my master's degree in analytical chemistry but synthetic organic chemistry for PhD. how to determine syn:anti from hplc spetra?

@munirothmanali9461

3 жыл бұрын

Hi sir, I catch you very nice but my question is to you is you said that higher peaks doesn't mean higher concentration .how? And if you recall in normal way as compound conc increase its give higher peaks.