HOW TO READ A CHROMATOGRAM (Step-By-Step Guide For Beginners)
The only thing you will need to know about how chromatography works to follow this video, is that they all separate compounds based on how they interact with the stationary phase inside their column. This causes the different sub components of the sample to exit the column at different times and whenever something exits the column it is measured.
The result from a chromatography is displayed in a chromatogram. The chromatogram gives you a well of information, both in terms of the health of the gas chromatography device in addition to data which can be used for qualitative or even quantitative analysis.
The retention time is displayed on the x-axis and is the time it takes the sample, from when it was injected, to run through the column and exit. The y-axis is the measured response of the analyte peak in the detector. The baseline shows the signal from the detector when no analyte is eluting from the column. This baseline should be low and straight. A higher and/or irregular baseline compared to what is normal indicates a problem or alternatively that maintenance of the device is required. By comparing the different peaks of our sample in terms of retention and shape to that of known compounds we can identify the different compounds present in our sample. Furthermore, the concentration of a compound can be determined by taking the peak area and comparing it to an example calibration curve of the same compound at different concentrations.
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What would you like me to cover next?😇
Very concise and helpful. Thank you!
@biotechlucas4126
Жыл бұрын
Happy that I could help!👍
Where to find the calibration curve of the same compound?
Perfect video. Thank you. Can you cover the analysis of different human chromatogram data (sanger) with a longer video?
@biotechlucas4126
Ай бұрын
I'll add it to my to-do list!😇👍
@ludvahve
Ай бұрын
@@biotechlucas4126 thank you 🙏😇
Is the elution time value read from the middle of each peak or the start of each peak ?
@biotechlucas4126
6 ай бұрын
So it's actually read from the time it enters the chromatographic column to when it starts eluting. Hope that helps!
Good
So how do you understand the numbers the machine puts out? I thought that's what this video was about.
@biotechlucas4126
9 ай бұрын
This is an excellent question that I unfortunately don't have a simple answer to. I'll make a follow up video in the future where I explain this!👍
Please help us by live demo using chromatography techniques it will easy understand and effective
@biotechlucas4126
3 ай бұрын
Sadly I don't have access to these instruments at the current point of my life. Will make sure to do such demonstrations if an opportunity arises in the future!
Thx but pls get a foam cover or mesh to not breath into the mic that load
@biotechlucas4126
Ай бұрын
Thank you for the feedback! I'll make sure to upgrade my audio in the future!
Pls can u do m+1 and m+2?
@biotechlucas4126
Жыл бұрын
I'm not sure what you are referring to🤔😅 Could you elaborate please 😇👍
@sophiechritton630
Жыл бұрын
@@biotechlucas4126 mass spec peaks