What is a monoculture, haploid, spore, multispore, monokaryon, dikaryon, swab, LC, MSS, T0, isolate?
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Пікірлер: 36
Yeees just put on the camera and start talking - that's it! 😍 Love the loads of videos you're bashing out 🔥🔥🔥
bro i love your personality, its like adhd on crack but i understood everything!
You’ve been killing it lately Ed! Absolutely love all you do!
🎉😊
Great great video Ed! I’m learning 😂
nice one again. can you maybe do a video on reviving dried fruits? keep it up brother. really appreciate your videos
The docta 👨⚕️ always spittin facts.. loads of info and very fast paced. Loved this.
Awesome info. Thank you 🌞🍄
The more you know!! Love it Ed!!
Great info Ed Thanks for the explanation
Thanks for the info! I'm getting smarter by
Very interesting. Thank you
that clears things up a lot, thanks Ed!!!
Thanks Ed
I love the topic, definitely needed
Thankyou for clarifying all the naming conventions and explaining them properly. Im sure ive got it wrong many times with only 6 months experience..btw ive added tou on FB.
Thanks Ed your knowledge is always spot on top notch. Love your work always 🙏🍄👍
Thanks for the video. I'm just getting into agar and this helps.
Thanks Ed...learning a ton thanks to your vids. Was wondering if you can describe (or do a vid on) visually identifying an monokaryon vs. a dikaryon on a plate? Thanks!
@edwardgrand
5 ай бұрын
I have. It is very hard to do the videography with my setup.
@edwardgrand
5 ай бұрын
Monokaryons don't have clamps at any septa. Dikaryons have clamps at some or many septa.
What is the benefit of a monoculture vs. any other.
Thanks bro its hard to find all this information in one place. 🍄❤️
What if they didnt isolate the culture before making an LC, and they just added piece from the first plate ? Another question, we propagate the culture many times so we can get a monoculture, which , if visibly described, would look like a single propagation of hyphae ?
@edwardgrand
3 ай бұрын
It would just be a mixture of cultures, most likely fighting with each other. A monoculture should have uniform, radial growth with no sectoring. Most of the time...
Question Mr. Ed if you happen to see this....if I was still green as hell and wet behind the ears but interested in dipping my feet into the mycology world a bit more with the purchase of new scope, what would you recommend as a good practice or use for said scope when first starting out. What should I be using the scope for if I'm just a backyard, homegrown, self taught hobbyist looking to expand his understanding of this new hobby. Thanks in advance good sir!
@edwardgrand
Жыл бұрын
Amscope B120C. It is all you need for checking monokaryons, dikaryons, measuring spores, etc. If you get into identifying wild specimens, it is all you need. I think they are going for about $250 now. It will be the only scope you ever need to buy for your lifetime in Mycology. Hobby or semi-pro.
Hey Ed! Not sure how I missed this one until now, but I already learned all that from your numerous other vids :) One thing thought that I think might still be a little confusing for beginners, which I am, is that point you clarified on haploid/diploid nuclei... I know I was confused for a long time, think I figured it out and wanted to run it by you. Your note still kinda gives the feeling the term monokaryon is associated with haploid, and dikaryon with diploid.. apart from the (). So just to be clear, would it be fair to summarise this way? Two haploid monokaryons mate to form a haploid dikaryon (each nucleus still contains half the genetic material the final fruit will be made of). We only see diploid nuclei after karyogamy and before meiosis, in the basidium, during the process of spore creation. And that is the only stage at which cross-over occurs, as in the genetic roulette :) I'm sure this is a crude summarisation for a scientist like you, but you get what I mean.. Anything hurting your eyes in there?
@edwardgrand
3 ай бұрын
Yes. That is correct. The only part is about calling the new dikaryon a 'haploid'. Haploid implies a cell that on had half of the normal genetic compliment. i.e. 'n', not n+n, 2n, 3n, etc. I have heard other scientists call it a 'polyploid', 'heterokaryon', 'multi-nucleate', etc. I would stick with calling it a dikaryon which is a special term we have in fungi. It eliminates any ambiguity. Sounds like you have been learning very well. This is precisely what I want to do with all these videos. You all will be making the videos soon. :) Thank you for watching!
@starburstmemories
3 ай бұрын
@@edwardgrand Thank you for teaching us well!
Ed, learning so so much. Thank you. Going to switch over to you bagging system. How do you sterilize: PC or just steam at ambient pressure ?? Your bags sound like cellophane, So Amazon has these: 12” X 18” 1.1 Mil LDPE Open Ended & Flat Clear Poly Bags, glossy, THIN & LIGHTWEIGHT: Manufactured from virgin LDPE (low-density) material. HEAT SEALABLE ( impulse sealer ), 100% FOOD SAFE If these are not suitable or the same please would you be kind enough to provide an accurate description of the bags you use. Thx again.
@edwardgrand
Жыл бұрын
The bags have to be PP5 to hold up in the PC. Grain must be sterilized in PC or autoclave. Thanks for watching.
@StickyDankFingers
Жыл бұрын
@@edwardgrand perfect and short concise. Love it. Thanks again for saving me time, bags, and grain. I asked about pc or steam bc I had purchased some bags that melted ( ish ) and fused into one large block. What a pain that was :(
Still very lost listening to this. Only because i don’t know enough. Piece by piece.
@edwardgrand
Жыл бұрын
There's a lot to learn. Hope it helped. I've been doing this a while and learn something new every day. Always new stuff out there.
Sheding light on that terminology, makes me sound like I have a degree in mycology 😅