The differences between hybridization and phenotypical selection of isolates when breeding mushrooms

Ғылым және технология

In this vide Gary with Fresh from the Farm Fungi explains the differences between hybridization and phenotypical selection of isolates in mushroom breeding! MUSHLOVE To purchase our cultures visit our ETSY shop FRESHFUNGI www.etsy.com/shop/freshfungi

Пікірлер: 73

  • @williamworthington9420
    @williamworthington94203 жыл бұрын

    You're videos are extremely helpful and have way more information than most. Thanks for blessing us with your knowledge!

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    3 жыл бұрын

    MUSHLOVE

  • @michaelwelsh9700
    @michaelwelsh970010 күн бұрын

    Great explanation! It’s been such a cool journey integrating my lessons from mycology and incorporating them into academia and the study of insect pathogen fungi.

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 күн бұрын

    @@michaelwelsh9700 thanks for watching and following along!

  • @redapproves1330
    @redapproves13302 жыл бұрын

    Just binge watched all your videos. I really appreciate your scientific and methodical approach to mushroom cultivation. And I am so grateful for someone like you investing tremendous amount of effort and time to share these valuable knowledge. What I thought would've been a fun summer project growing shiitake have turned into a daily obsession of mine. There are so much to learn and so much yet to be discovered! Thanks Gary!

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    thanks for watching and following along!

  • @KellenChase
    @KellenChase3 жыл бұрын

    Fun! Thanks for sharing. I just saw someone who’d isolated a really funky looking phenotype and really had no idea what that meant. I appreciate your videos and I’ve been looking at mychorhizal stuff for my 2020 apocalypse garden... actually what led me to fungi in the first place. Really looking forward to what you’re bringing to fungi and gardening

  • @triple_gem_shining

    @triple_gem_shining

    2 жыл бұрын

    The more you know 🌈

  • @UrbanInsight
    @UrbanInsight3 жыл бұрын

    Excellent Information Gary🍄❤️🍄

  • @TheRoadtoGonzo
    @TheRoadtoGonzo3 жыл бұрын

    This was a really nice video. You don't see to many channels talking about isolating genetics. Is like to see a video or two in how selection, isolation and breeding is done 👍

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    3 жыл бұрын

    Thanks! We have a playlist “breeding mushrooms from spores” and it goes through the whole process thoroughly start to finish - from spores to stores! : )

  • @TheRoadtoGonzo

    @TheRoadtoGonzo

    3 жыл бұрын

    @@FreshfromtheFarmFungi thanks I did catch up eventually probably the best breeding material I've found on youtube after searching for some time, I'll shoot you guys an email I have a few quick questions 👍

  • @joeg7491
    @joeg74912 жыл бұрын

    Thank you so much for your hard work on breeding new varieties

  • @mosiahparkman358
    @mosiahparkman3583 жыл бұрын

    Very interesting information. Thanks for posting Gary!

  • @TheSporceror
    @TheSporceror Жыл бұрын

    I'm one of your biggest fans man. Thank you so much!

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    thanks for watching and following along! MUSHLOVE

  • @aaronramsden1657
    @aaronramsden16573 жыл бұрын

    Love your work

  • @gwaters8067
    @gwaters80673 жыл бұрын

    Thank you Gary. You don't know me but you have taught me loads already through your videos 👍 I am new to Mycology and your channel is a wealth of knowledge. Great work, Mush love from the UK

  • @pamelawillis9714
    @pamelawillis97143 жыл бұрын

    Excellent. There is a book in your future, Gary.

  • @petergoomba7681
    @petergoomba76813 жыл бұрын

    very informative channel!

  • @dizidnte
    @dizidnte Жыл бұрын

    excelent video De lujo, que buen video 💯🦅

  • @vitaly5209
    @vitaly52092 жыл бұрын

    that's really valuable information thanks a lot

  • @soccer2themax
    @soccer2themax3 жыл бұрын

    Thank you!

  • @soccer2themax

    @soccer2themax

    3 жыл бұрын

    Your channel is exactly the kind of channel i would create. Simple, friendly, informative

  • @gregmcb5305
    @gregmcb53052 жыл бұрын

    You should hydride a azurescens with something large like Ecuador strain. Or just normal cubes but either way it would be a verry nice thing. I know their food sources are different but that’s what would make this so interesting!

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    that would be an interesting combination indeed

  • @AnxiousStacker

    @AnxiousStacker

    Жыл бұрын

    I love fresh caps stuff but if I may Yoshi Amono does a lot of those exact types of breeding as well with a bunch of different actives.

  • @gustavosiles2048
    @gustavosiles20483 жыл бұрын

    how can i get a better genetic out of a lios mane spore syringe using agar? What caracteristics of the micelium should I pick or look for ? Thanks

  • @TheGoodFunGuy
    @TheGoodFunGuy3 жыл бұрын

    We don’t deserve you or the university level education you provide us with. Thank you! Would you have any interest in working on a collab project with a couple others in the mushroom community?

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    3 жыл бұрын

    thanks for watching! Sure send some more info to my email freshfromthefarmfungi@gmail.com I will see how I can help

  • @valerydan4206
    @valerydan4206 Жыл бұрын

    I am wondering is it possible to hybridize reishi with turkey tail mushrooms?

  • @Marlene5018
    @Marlene50182 жыл бұрын

    You are not a farmer, actually you are a scientific ! Great information, thanks for sharing ;)

  • @MycoPharm-Mycology
    @MycoPharm-Mycology Жыл бұрын

    Do you have a video that explains how to isolate a phenotype from a culture without sophisticated laboratory testing and equipment? What do you think is the best way for the home grower with only a very basic myco-lab (e.g., microscope, LFH) can achieve the best genotype of the species when starting from a spore? Lot of videos out that show you how to make a selected rhizomorphic culture but I haven’t seen any explain why we should do that and what it means in cultivation.

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    kzread.info/dash/bejne/a41sk9BtfKrafKg.html kzread.info/dash/bejne/Y6OlxcqhgcXecqQ.html these two videos may touch on this subject

  • @AntiTrollable
    @AntiTrollable3 жыл бұрын

    Can you please do a video on senescence of mycelium and whether agar to agar, for example, is cloning or just an "extension of growth" of the isolated genetics. It seems illogical to me that there would be a small limit to how many times mycelium can be transferred as, to my understanding, it is not the same as cloning. What I have read about it it seems the only reason it enters senescence is due to toxic DNA and mitochondrial breakdown and is a genetic flaw within the isolation itself and not the amount of tranfers. I don't know much about biology but this is just presumptuous logic. Would love your thoughts on this. Cheers.

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    it depends on the species and even the phenotype - some strains last years and years and some degrade quickly.

  • @Algumnomequalquer
    @Algumnomequalquer Жыл бұрын

    Gary, how to do clone a culture right without senescence ? How people keep a strain for so long without problems? There's no video about that. Mushlove

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    it’s best to store on slants and take from the mother culture watch the video on slants for long term storage kzread.info/dash/bejne/eIiWqrFroc-eiJM.html

  • @Iranmyco
    @Iranmyco9 ай бұрын

    What happened if we make too much of agar transfers?

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    9 ай бұрын

    watch this video kzread.info/dash/bejne/Y3l1x9msk9XVgMY.htmlsi=PDApYacABw6jfmtU

  • @vytautas.take2
    @vytautas.take2 Жыл бұрын

    Thank you, your videos are amazing. Im just getting confused here a little. You say that to get a consistent result you hold onto the 1st gen that you select. What is 1st gen? Any gen that came from spores? I literally think about the very 1st mushroom patch ever, lol, that's not helpful, haha. Jokes aside, I thought that no mater the selection or breed of the previous gen, spores will always have a clean slate and anything is possible because it has diversity that then you select from? Also, you say that strains loose vigour through cloning. But then how do I hold on to the same gen? Cloning is when you grow mycelium from mushroom tissue, but also when you share grain spawn and liquid cultures, right? Or the latter grain/lc methods are what you mean by holding on to a strain? Sorry for stupid tangled questions. I feel I have all the pieces, just need to place it right..

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    yes one key aspect is using slants for long term storage and refrigerating or using super cool temperatures and glycol to store in cryo freezers. slowing down replication to preserve the genome as much as possible - hope that helps makes things clearer!

  • @bradmcclure4945

    @bradmcclure4945

    Жыл бұрын

    what approach for hybridizing are you planning to use I'm planning to try hybridizing of different phenotypes by basically using two different spore samples in my cloning process

  • @chrisroio1219
    @chrisroio1219 Жыл бұрын

    Do I get the snake venom from the snake oil salesman? LOL

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    No

  • @chrisroio1219

    @chrisroio1219

    Жыл бұрын

    @@FreshfromtheFarmFungi lol. Do you have any new videos?

  • @tat2gawd
    @tat2gawd Жыл бұрын

    What about explaining an agrarian permaculture

  • @vgames6792
    @vgames67922 жыл бұрын

    bought 10ml of lions mane. 7days ago, i made out of it: 10 petri dishes with single drop, nothing. 2x 2ml into 750ml millet grainspawn, nothing. 3 x 750ml liquid culture with the rest. One of them no grow(bits are floating), 2nd minimal grow, 3rd look created a single line spiral no network, may contaminated by something. So gonna have to try again. But the shitake i bought from the same guy colonize like crazy, compare to the one i already had. So genetic definitely make observable difference.

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    the lions mane culture I have is wispy on agar and may not look like much until it fruits - that being said it could have been damaged during shipping or it tends to clump in the syringe. It’s best to vortex it first or use the whole syringe on a grain jar to get it going. Message me on ETSY and I can send a fresh one Tuesday I have been using the same Lc all year and getting good fruits.

  • @vgames6792

    @vgames6792

    2 жыл бұрын

    @@FreshfromtheFarmFungi from EUrope dont have much faith it will get here alive + not sure how is it with customs BS :(

  • @bradmcclure4945
    @bradmcclure4945 Жыл бұрын

    if I combine the liquid cultures from two different strains will it result in a hybrid???

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    no they will compete. They need to be genetically manipulated or start from spore

  • @bradmcclure4945

    @bradmcclure4945

    Жыл бұрын

    @@FreshfromtheFarmFungi which begs the question of how do you genetically manipulate the mothers

  • @bradmcclure4945
    @bradmcclure4945 Жыл бұрын

    does genotype degradation happen when cloning

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    Жыл бұрын

    it may or may not - it depends if the mycelium mutates - my personal opinion is that it can randomly mutate from physical stress like cutting, but some strains are more tolerant than others

  • @powerslohner6728
    @powerslohner67282 жыл бұрын

    What if you mix syringes?

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    only a few exceptions could mate from Dikaryotic to monokaryons spontaneously otherwise they will compete with eachother

  • @powerslohner6728

    @powerslohner6728

    2 жыл бұрын

    @@FreshfromtheFarmFungi So it would be a waste

  • @emrysmcwryn7902
    @emrysmcwryn79025 ай бұрын

    1:58

  • @ronanclark2129
    @ronanclark2129 Жыл бұрын

    Snake venom forces crossing over??

  • @samjanssens1509
    @samjanssens15095 ай бұрын

    dont forget breathing my guy

  • @MalinaImport
    @MalinaImport3 жыл бұрын

    senescence is only a thing with cordy. with gourmet species it's not an issue if you feed the myc different food cycles (different grains, different agar recipes)

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    3 жыл бұрын

    It may be prolonged with these methods yes, but all strains eventually senesce - one way or another the G1 will always be the strongest unless a random mutation occurs along the way that selects for a desired trait. It’s just nature

  • @MalinaImport

    @MalinaImport

    3 жыл бұрын

    @@FreshfromtheFarmFungi thanks for replying :)

  • @FRESNEL_COOKING_SOLAR_OVENS24
    @FRESNEL_COOKING_SOLAR_OVENS242 ай бұрын

    I recognise you always breathing very hard. Try no to spell long phrases between breathing air. Your lungs seem underdeveloped for this. You may have formaldehyde deposits in your lungs. You could develop some issues soon, depends how did you handle contact with smoke, spores, dusts, asthma, chemicals, vapours, gases. Check your lungs in 3 different clinics.

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 ай бұрын

    I appreciate your concern I usually do these videos in one take between inoculation batches and Im very active - I have good doctors and am in fact healthy and can run miles at a time without issues - mushroom farming is a labor of love and filming is above and beyond so I do my best to get the info out there 🙏🏻

  • @jonnypage9484
    @jonnypage94842 жыл бұрын

    I'm not trying to tell you how to do your job because both of us are from good old-fashioned piece of shit New York I'm like 40 minutes away from Buffalo buddy but for real I can feel your nervousness and this video every time you take a deep breath shit freaks me out for some reason and then you looking down to the camera idk 😐 bro relax it's okay

  • @FreshfromtheFarmFungi

    @FreshfromtheFarmFungi

    2 жыл бұрын

    thanks for the feedback It’s actually after I got done working out I often get ideas when I work out and film them in one take so I don’t forget but yes I agree it wasn’t ideal

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