Пікірлер

• 
  @thelukesternater22 сағат бұрын

  With how you pouring them plates I’m in shock

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  @BigfootPrinting2 күн бұрын

  Hey Gary, I’ve really been enjoying these videos. You seem to be extremely well educated and a good presenter. May I ask if you know of any books or other robust resources that I could study to learn about this genetic/breeding/microbiology stuff pertaining to our mycology contexts? KZread is a great resource, but I really like an authoritative sources like books

• 
  @sacredatavism4 күн бұрын

  I'm just now seeing this, but I just wanted to say I appreciate so much that you did the work. There's a lot of old "wisdom" that gets repeated uncritically by people, and it's important to test this stuff and see what holds up and what doesn't.

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  @astroranger55284 күн бұрын

  In your lab, what are you / were you using as injection ports on the bags, or did they already come attached, like they do with unicorn bags?

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  @carl-cr7rp4 күн бұрын

  This is good information. Thanks.

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  @matthewpetersen44174 күн бұрын

  Once figured out and done right mushrooms are very easy to grow and actually fun to grow! I keep keeping spores and cultures and its a never ending cycle of growing. Love growing on agar!!!!!!!!!

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  @bobbyboergerhoff96485 күн бұрын

  9:26 that doesn’t look like dryad, saddle, or pheasant back, to me. It looks like sarcodon.

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  @BigfootPrinting6 күн бұрын

  May I ask what your recipe is? I think I’m adding too much nutrient or agar as after 10 mins of boiling and mixing it’s still cloudy and when put into the jar I can see things falling out of solution.

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  @FreshfromtheFarmFungi6 күн бұрын

  try getting lab grade agar it should dissolve well

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  @BigfootPrinting5 күн бұрын

  @@FreshfromtheFarmFungi it turned out being a skill issue, I finally got some crystal MEA plates poured. You’re the man, thanks! Btw, if one doesn’t have a laminar flow setup to leave plates over the weekend in, just a SAB, is there anything that can be done to dry the condensation out of the plates without risk of contam?

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  @FreshfromtheFarmFungi5 күн бұрын

  @@BigfootPrinting yes , it’s all about temperature differential, so try warming up the SAB or placing in a warm room - when the plates cool they are hotter than the air around them which makes the water condense on the lids. You can even try pre-warming the plates somehow - hope that helps!

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  @marsjuzeew12867 күн бұрын

  Hallo Geri, ich klone seit 20 Jahren Steinpilze und Trüffelpilze, ich kann Ihnen Tausende von Setzlingen verkaufen

• 
  @TheBloomingSelf9 күн бұрын

  Super glad I found your channel. Thank you for the great info.

• 
  @jennymossa94929 күн бұрын

  Plastic chopping board and even a plastic strainer....

• 
  @MyHomeUSA9 күн бұрын

  I'm sorry to be the bearer of bad news but, if it's a closed closet to which you applied the positive pressure HEPA filter/blower, then It's a closed loop, and you've added neither positive nor negative pressure to affect in your lab! The closet will draw air from your lab into the vacuum environment within, through every conceivable draft opening around the door. In fact, you've added an Ineffective air purifier to the lab, and that's because it will steer up resting closet particles which will become airborne and which the HEPA filter will need to suck up...

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  @FreshfromtheFarmFungi9 күн бұрын

  @@MyHomeUSA it’s not a closet I should have shown what’s behind the door lol it leads into the larger area of the basement which has access to the upstairs so the air comes from above and down through the stairwell, through the “closet” which is a utility area through the filter and into the room. Hope that makes sense!

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  @AtanasMukoski-ji5qe9 күн бұрын

  Can you pls tell me after soaking do you cook the grains? Rye berry ? How much time do you cook?

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  @FreshfromtheFarmFungi5 күн бұрын

  @@AtanasMukoski-ji5qe yes they are sterilized for 60 minutes at 250F/15PSI - I prefer oats but this will work with most grains

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  @ZeginMakesMusic10 күн бұрын

  I recently poured my own agar plates. I was terrified that it wouldn't work because I don't even have a pressure cooker. I literally just boiled it inside of a Starbucks bottle. Using a still air box and a alcohol flame, I inoculated about six plates. I was so worried about contamination. 10 days later and I have beautiful growth on three plates, The other three are completely avoid of anything. I used a drop of liquid culture and may not have gotten any actual mycelium on the plate. But no contamination yet. I feel like a true scientist, and I didn't even wash my hands.

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  @FreshfromtheFarmFungi5 күн бұрын

  @@ZeginMakesMusic nice work 👍 before pressure cookers and fancy flow hoods people were able to get success this way. It can be done and is a good building block for technique

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  @bencll10 күн бұрын

  Thank you brother.

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  @TylerHanke-he5kx10 күн бұрын

  how does the vortex machine work? there is no magnet like a stir plate?

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  @FreshfromtheFarmFungi5 күн бұрын

  @@TylerHanke-he5kx it just vibrates in a circle when pressure is applied - no magnets 👍

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  @NotaFEDjed11 күн бұрын

  Why not use a syphon instead of a special pump?

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  @FreshfromtheFarmFungi5 күн бұрын

  @@NotaFEDjed you can use a syphon at this scale but the peristaltic pump is a closed system so it can be scaled up in a sterile environment

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  @michaelwelsh970013 күн бұрын

  Great explanation! It’s been such a cool journey integrating my lessons from mycology and incorporating them into academia and the study of insect pathogen fungi.

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  @FreshfromtheFarmFungi5 күн бұрын

  @@michaelwelsh9700 thanks for watching and following along!

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  @cuddlybear452413 күн бұрын

  Hey Gary, any recommendations for a stationary co2 meter for a Martha tent?

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  @FreshfromtheFarmFungi12 күн бұрын

  yes these did not exist when I made this video and they are the best value right now - amzn.to/3VTyR3D

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  @konstantinNeo13 күн бұрын

  Could you please talk about peptone usage? Types of peptones you have experience with.

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  @ZeginMakesMusic14 күн бұрын

  I've been using a small alcohol burner inside of my still air box. I'm never in there long enough to start a fire and I always wait until the alcohol evaporates. Is there any reason why I shouldn't do this? Do tiny microplastics dissolve from the top? I'm only a week in and can't really see any contamination on anything. I've been trying to clone mushrooms from the grocery store onto plates. So far so good.

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  @FreshfromtheFarmFungi13 күн бұрын

  yes this can work but I avoid open flames because of the fire risk. It just takes one bad accident to cause damage but if you are careful it can work - this is how microbiologists worked before the modern innovations

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  @eliasdskill500815 күн бұрын

  Ty for helping my journey!

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  @ZeginMakesMusic15 күн бұрын

  3 days ago, I put a piece of sterile tissue from a lion's mane mushroom onto an agar plate. There is no growth at all. I soaked it in hydrogen peroxide first. Did I kill it?

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  @FreshfromtheFarmFungi15 күн бұрын

  it should be ok as long as it was a large enough piece but ideally if you use sterile tissue you shouldn’t need to soak it - Be patient I have faith it will emerge 🙏🏻

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  @13LuckyykcuL1315 күн бұрын

  It's pronounced Ay-gr

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  @FreshfromtheFarmFungi15 күн бұрын

  @@13LuckyykcuL13 in formal education it is pronounced how I pronounce it - a lot of internet teachers pronounce it like that - apples to apples in my opinion

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  @ineshianewton774015 күн бұрын

  Hello could you tell me how to clean a monotub that had trichoderma?

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  @FreshfromtheFarmFungi15 күн бұрын

  I recommend soaking in a bleach solution for at least 20 min (I usually use a larger bin filled with 10% bleach and soak overnight) then dry off and wipe with isopropyl alcohol you will be good to go 👍

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  @ineshianewton774013 күн бұрын

  @@FreshfromtheFarmFungi ok thank you...and will the bleach give instructions to make 10% solution?

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  @nuclearcooties967915 күн бұрын

  I prefer LC over agar. I just hate it when my dishes go septic. Then have to open those up to clean it out and it reeks. I have a preference for growing oyster mushrooms because they will grow almost to spite you. You can start out on agar then go to LC. But in general I try to avoid agar whenever possible. Instead of stir bars I use marbles. I can't justify spending the 40 bucks on something that I can do manually.

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  @FreshfromtheFarmFungi15 күн бұрын

  @@nuclearcooties9679 I think there is value in both - liquid culture also can get contaminated and it just hides itself so be aware of this - agar is a filter for finding contams

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  @BigfootPrinting16 күн бұрын

  Can the camera be used with the 25x oculars that the scope comes with for greater mag? Or is that only possible for direct viewing

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  @FreshfromtheFarmFungi15 күн бұрын

  @@BigfootPrinting It doesn’t fit mine but maybe there is an adapter Im not using that came with it

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  @theguyfromMaine20717 күн бұрын

  how do you get airflow if you just have a closet? I cut two small slits in the bag. do I need a fan?

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  @FreshfromtheFarmFungi17 күн бұрын

  Make micro compartments/micro climates - I would do two separate areas and have one be for fruiting and one for air exchange/treatment - look up the “martha tent” setup 👍 you don’t need much air flow for smaller batches

• 
  @Armpit5417 күн бұрын

  Please tell me the brand and size of your boxes. I am just producing mushrooms from my 160 block mushroom farm and want to start packaging my produce with the best containers. Thank you!

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  @FreshfromtheFarmFungi15 күн бұрын

  We get these second hand from our foragers but they are known as “tomato flats” in the markets you can get them from ULINE I believe

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  @localbroadcast18 күн бұрын

  speedy OG! Awesome work /w your breeding work going above n beyond the call of duty!

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  @localbroadcast18 күн бұрын

  Bro, @30:30 Are those cubes you bustin' out for us to gaze upon??? If that be for real for reals then I tip my hat to you my good man as that is one helluva cluster packed ball of wonder!

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  @SERGEYFT19 күн бұрын

  Do Cordyceps spores last long? Is it possible to make a print out of them and store them for years?

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  @FreshfromtheFarmFungi19 күн бұрын

  no, this is a huge challenge with cordyceps and a lot of other ascomycetes. I believe it is nature’s way to balance these mushrooms in the wild

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  @SERGEYFT18 күн бұрын

  @@FreshfromtheFarmFungi Thank you

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  @doggodoggo300019 күн бұрын

  i saved a really large carolina wolf spider in my house one winter. It was too big to leave it be and too cold outside to throw it out there and exprect it to live. So I prepared a habitat for it to live out the rest of its life. it always was under a hide i placed in there but one day it climbed on top and was reaching for the sky with its front legs. it died like that and it started growing a fungus. it reminds me of the old stories about ants that wouild get infected and climb trees. but this is much bigger than an ant. hogna carolinensis is a pretty big spider. i joke i had a pet zombie spider for a while.

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  @FreshfromtheFarmFungi19 күн бұрын

  hahh awesome! This may very well be 🍄❤️

• 
  @spacecat8620 күн бұрын

  Just got some of your piopino lc :) cant wait to try it out

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  @FreshfromtheFarmFungi19 күн бұрын

  awesome! Happy Growing! 🍄❤️

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  @SERGEYFT20 күн бұрын

  Do Cordyceps militaris spores last long? Can you use them to make a spore print on foil and store it for years? Or should the spores be planted immediately since their shelf life is short?

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  @FreshfromtheFarmFungi15 күн бұрын

  the cordyceps spores are ascospores and are fragile so they should be used fresh as quickly as possible

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  @GraftingAvocados21 күн бұрын

  Were you able to breed cordyceps to be more pathogenic to specific insects?

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  @FreshfromtheFarmFungi19 күн бұрын

  I could not keep this culture alive very long - I believe spores would be more effective but they are difficult to accumulate but I will be attempting more experiments like this in the future

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  @GraftingAvocados18 күн бұрын

  @@FreshfromtheFarmFungi Thanks interesting concept. Chat GPT has some good references.

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  @GraftingAvocados21 күн бұрын

  interesting Idea that you can train entomopathogenic to consume specific insect.

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  @FreshfromtheFarmFungi15 күн бұрын

  yes there is an industry based on this

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  @kallencooke330722 күн бұрын

  Hey mate where can I get those racks that hold your bags when fruiting they are awesome

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  @Nightowl_IT23 күн бұрын

  You gotta mention the mushroom You are using. Only use non "edible" and non toxic substrates. Grains are a waste of food. Organic saw dust from untreated wood is an option or bran like useable waste products.

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  @jasonlockhorst150524 күн бұрын

  Brother Gary, your the best! But I really want to write to you regarding that you look into being more diligent when spraying iso alc, particularly when the hood is on, projecting the spray back onto you and into your breathing area. I used iso just to clean my surface areas for a few years, then I got tested for health reasons, indirectly, they found iso built up in my system. From watching your videos I see that you are a lot more exposed to iso than I was. I just wanted to share this with you in regards to long term health. Absolutely love your sharing Gary. Thank you

• 
  @a23meme17 күн бұрын

  this is something that has concerned me for the past couple of months. what did you do next ? maybe a respirator while working ?

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  @FreshfromtheFarmFungi15 күн бұрын

  Yes this should be considered - I think wearing gloves and tyvek sleeves could help and also some people prefer ethanol but I just try to keep the air clean in my work space the best I can - working in the lab I have been exposed to lots of volatile chemicals I could be better with PPE

• 
  @ZeginMakesMusic25 күн бұрын

  Is it possible to manufacture some sort of pest control application with the cordyceps spores or culture?

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  @FreshfromtheFarmFungi15 күн бұрын

  yes I believe so - in my experience the cultures usually die off quite rapidly which is a challenge to overcome

• 
  @RynaxAlien26 күн бұрын

  Is any air purifier capable of laminar flow air?

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  @FreshfromtheFarmFungi15 күн бұрын

  yes - but specifically the ones designed for lab work are laminar because the currents are all unilateral and directed in a way that makes it uniform.

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  @RynaxAlien14 күн бұрын

  @@FreshfromtheFarmFungi I want to use Bionaire air purifier to make laminar flow hood

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  @pacman353627 күн бұрын

  What an awesome video. Very helpful, and I can’t imagine how much work that took to make

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  @BeardedHillbilly29 күн бұрын

  It's definitely a pro tip. Doesn't help the poor man any, lol.

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  @FreshfromtheFarmFungi15 күн бұрын

  it’s all about the temperatures! Just try to keep things as close as possible to the temperature of the agar

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  @pacman353629 күн бұрын

  Slurping coffee is annoying. Otherwise, I love your videos….

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  @FreshfromtheFarmFungi14 күн бұрын

  Indeed - I do these in one take so sometimes I get carried away but this is an oldy

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  @unit333angel29 күн бұрын

  woooooooooow

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  @Pavel_Gribovod29 күн бұрын

  Круто. А где результат посмотреть?

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  @FreshfromtheFarmFungi14 күн бұрын

  we got pins but they dried up quickly - we will be scaling this in our new spot

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  @Pavel_Gribovod13 күн бұрын

  @@FreshfromtheFarmFungi Thanks!!! good luck!!

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  @ZeginMakesMusicАй бұрын

  Lets say i inject GT spores into a JediMF grain spawn. Would that form into a hybrid, grow both types of mushrooms, or fail and grow none?

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  @FreshfromtheFarmFungi14 күн бұрын

  there is a phenomenon called di-mon pairing that could occur but it is random and no way to verify without doing genetic tests. I am not sure if cubensis will do this in grain spawn or not but it’s worth a shot right? I believe ed garrand has some good content on this as well

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  @mrnobody1456Ай бұрын

  Great vid. I just wish you have used some brown rice as well

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  @FreshfromtheFarmFungi14 күн бұрын

  I left it out because my experience it tends to get sticky and mushy - however maybe in the next go around I can try this 👍

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  @DawidKellermanАй бұрын

  Thank you for the Effort and Work!