Our scientists demonstrate the full assay procedure of Dietary Fiber (AOAC Method 991.43 / AACC method 32-07.01) using Megazyme's Total Dietary Fiber Assay Kit, (K-TDFR)
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This Assay Kit can be found on our website: www.megazyme.com/total-dietar...
00:06 Introduction
02:31 Principle
04:27 Preparation of Fritted Crucibles
04:44 Sample Preparation
05:43 Reagent Preparation
06:12 Weighing of Samples
06:35 Incubation with heat stable α-amylase
08:24 Incubation with Protease
09:35 Incubation with Amyloglucosidase
10:18 Method A - Measurement of TDF as HMWDF
14:44 Method B - Separation of TDF components into IDF and SDFP
14:48 Measurement of IDF
18:01 Precipitation & Recovery of SDFP component
19:19 Calculations
Download the K-TDFR assay protocol: support.megazyme.com/support/...
Access the Mega-Calc for K-TDFR: support.megazyme.com/support/...
Supporting Video: Preparation of crucibles with Celite Filter Beds • Preparation of Crucibl...