www.megazyme.com/beta-glucan-...
Comprehensive Technical Video Protocol for Megazyme's Mixed Linkage Beta-Glucan Assay Kit (K-BGLU)
This Beta-Glucan Assay Kit method is validated under collaborative study (AOAC method 995.16 and AACC Method 32-23.01, CODEX Method Type II, EBC Methods 3.11.1, 4.16.1 and 8.11.1, ICC Standard No. 166 and RACI standard method) for the measurement of 1,3:1,4-ß-D-glucan in cereal grains, milling fractions, wort, beer and other food products.
** Subtitles are available but must be manually turned on, and preferred language chosen **
Chapters for this video can be found below:
00:03 Introduction
00:48 Principle
01:37 Reagent Preparation
04:50 Weighing of samples
06:02 Gelatinisation of sample
07:08 Lichenase depolymerisation of β-Glucan
08:30 pH adjustment & incubation with β-Glucosidase
10:30 Glucose Determination (GOPOD Reagent)
12:04 Calculations
14:30 Further information
• Product page at the Megazyme webstore:
www.megazyme.com/beta-glucan-...
• Download the K-BGLU data booklet: support.megazyme.com/support/...
• Access the Mega-Calc for K-BGLU: support.megazyme.com/support/...