www.megazyme.com/rapid-integr...
The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AOAC Method 2017.16, ICC Standard No. 185) and suitable for the measurement and analysis of Total Dietary Fiber as per Codex Alimentarius definition.
** Subtitles are available, but must be manually turned on and can be changed to your desired language **
00:05 Introduction
04:45 Principle
06:46 Reagent Preparation
Procedure
09:45 Weighing of samples
10:13 Incubation with PAA and AMG
11:27 Preparation of Reagent Blanks
11:48 pH Adjustment & protease treatment
13:59 Alcohol Precipitation of HMWSDF
Method A - Measurement of TDF as HMWDF + SDFS
14:36 Filtration and separation of HMWDF (IDF + SDFP) and SDFS
17:21 Aa. Determination of HMWDF (IDF + SDFP)
18:31 Ab. Determination of SDFS
18:35 (i) Recovery, sub-sampling and concentration of SDFS
22:29 (ii) Chromatography of samples on HPLC
23:50 Calculations of TDF - as HMWDF (IDF + SDFP) plus SDFS
Method B - Measurement of TDF as IDF + SDFP + SDFS (allowing determination of IDF and SDFP + SDFS separately
27:06 Digestion of samples
27:50 Separation of IDF from SDFP & SDFS
30:29 Ba. Determination of IDF
31:15 Precipitation and recovery of SDFP
32:00 Bb. Determination of SDFP
32:21 Bc. Determination of SDFS
32:48 Calculations - TDF as IDF plus SDFP plus SDFS
• Product page at the Megazyme webstore:
www.megazyme.com/rapid-integr...
• Download the K-RINTDF data booklet:
support.megazyme.com/support/...
• Access the Mega-Calc for K-RINTDF:
support.megazyme.com/support/...
• Supporting video: Preparation of crucibles:
• Preparation of Crucibl...