Technical Video Protocol for Megazyme's Digestible Starch & Resistant Starch Assay (K-DSTRS)
A procedure for the specific measurement and analysis of digestible and resistant components of starch with a single kit.
** Subtitles are available, but must be manually turned on and can be changed to your desired language **
00:05 Introduction
01:42 Principle
04:11 Reagent Preparation
07:54 Enzyme Digestion: Method 1 - Analysing ~ 0.5 g of sample
09:21 Method 1 (A) - Addition of PAA + AMG solution
09:50 Method 1 (B) - Addition of PAA + AMG suspension
10:23 Enzyme Digestion: Method 2 - Analysing ~ 1.0 g of sample
11:34 Method 2 (A) - Addition of PAA + AMG solution
12:03 Method 2 (B) - Addition of PAA + AMG suspension
12:33 Preparation of Stopping Reagents
13:12 Determination of Digestible Starch
18:27 Determination of Resistant Starch
25:53 Calculations
This video will talk you through the assay principle, procedure, and will also explain how to perform the necessary calculations.
• Product page at the Megazyme webstore:
www.megazyme.com/digestible-a...
• Download the K-DSTRS data booklet:
support.megazyme.com/support/...
• Access the Mega-Calc for K-DSTRS:
support.megazyme.com/support/...