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Comprehensive video showing the analysis of Dietary Fiber (AOAC Method 985.29 / AACC method 32-05.01) using Megazyme's Total Dietary Fiber Assay Kit, (K-TDFR)
** Subtitles are available, but must be manually turned on and can be changed to your desired language **
00:03 Introduction
02:16 Principle
Method Pre-Requisites
03:34 Preparation of Fritted Crucibles
03:52 Sample Preparation
04:50 Reagent Preparation
Procedure
05:19 Weighing of Samples
05:44 Incubation with heat stable α-amylase
07:15 Incubation with Protease
08:11 Incubation with Amyloglucosidase
Measurement of “Prosky” Total Dietary Fiber as HMWDF
09:18 Precipitation of High Molecular Weight Soluble Dietary Fiber
09:59 Filtration & Washing of Dietary Fiber (HMWDF)
12:54 Determination of “Total” Dietary Fiber (HMWDF)
14:13 Calculations
Product page at Megazyme Webstore: www.megazyme.com/total-dietar...
Download the K-TDFR data booklet: support.megazyme.com/support/...
Access the Mega-Calc for K-TDFR: support.megazyme.com/support/...
Supporting Video: Preparation of crucibles with Celite Filter beds • Preparation of Crucibl...