Testing and Screening Protocol for Hop Latent Viroid (HLVd)
Testing and Screening Protocol for Hop Latent Viroid (HLVd)
Professor DeBacco
Tassa Saldi, PhD: Interview
We The Growers. (2022, March 17). Tassa Saldi, PhD - CoFounder and CEO of TUMI Genomics - E.11 [Video]. KZread. • Tassa Saldi, PhD - Co...
Leaves Was the Standard
This does work
Petioles are slightly better
However, latest data indicates early accumulation in the root tissue before it saturates the plant and will show-up with reliability in the leaves throughout the plant.
Mother Plant (2 months and older)
Four leaf/petiole samples at different areas of the plant.
Systematic sampling throughout the canopy.
Root samples can be hard to get and do physical damage so leaves/petioles are preferred for this stage of plant development.
2 Months and Younger
Go for a root sample
This is because of the early accumulation here, the viroid load will likely be elevated improving the odds for detection on a young plant.
Frequency… Depends
Are you going to be keeping and/or cloning the plant?
Ideal window is 2-4 weeks to ensure continual screening to catch something early
2 weeks = best for actively growing plants
4 weeks = best for established plants
Plant Quarantine
If you bringing plants in there should be a 30-day quarantine to allow for proper evaluation and screening.
This may require multiple screenings to ensure only “clean” plants are brought in as part of the growing operation.
If You Get a Positive…
Isolate the plant and remove it
Full plant tag, bag and disposal is advised.
Remember it is transmitted by physical damage and sap transfer.
Also, the roots can have some of the highest viroid loads be avoid transfer contact of these as well.
Link to Lecture Slides: drive.google.com/file/d/1RKqf...
*Due to the description character limit the full work cited for "Testing and Screening Protocol for Hop Latent Viroid (HLVd)" can be viewed at... docs.google.com/document/d/1t...
Пікірлер: 23
I can not thank you enough professor. Honestly 🙏
@DeBaccoUniversity
10 ай бұрын
Glad to hear that and great to know the contact is appreciated!
I need to go back to school, I've been growing for 32 years and half the words he says I have to look up...
@DeBaccoUniversity
10 ай бұрын
Life long learner!
More great content as usual! Thanks again!!
@DeBaccoUniversity
10 ай бұрын
Glad you enjoyed it and thanks for the subscription!
Yet again thank you very much indeed for this information you have given us on this topic. Have a beautiful day 🫵🏻👍🏼
@DeBaccoUniversity
10 ай бұрын
You are so welcome great to see your continued support of the channel!
I like the fungi network in the soil and the interactivity of symbiotic plants root systems, They share resources. Canibus ,tomato's and canus lily are symbiotic and collect different nutrients from the air and share them!
@SkunkPunch73
10 ай бұрын
Well just hope you don’t get Hlvd into your mix’s or I can assure you you won’t be liking that!
@DeBaccoUniversity
10 ай бұрын
It is great to have this symbiotic relationship, but it does not offer any protection against HLVd.
Great content professor. Keep it growing 😊
@DeBaccoUniversity
10 ай бұрын
Appreciate it!
Would be nice to get a home testing kit. Would this be possible?
@DeBaccoUniversity
10 ай бұрын
Currently best done in a lab under controlled conditions, especially when the viral load is small.
Hi professor- If a large outdoor plant tests positive does the soil also need to be removed in order to protect future plantings? The plants are growing in 350 gallon smart pots.
@max420thc
10 ай бұрын
Yes
@DeBaccoUniversity
10 ай бұрын
Ideally to ensure no cross contamination... yes.
@tayytes
10 ай бұрын
Yikes, what a harsh reality. That's going to get expensive.... any hope of developing future remedies for this viroid?
What are the best tests you would recommend that are the most reliable? Correct me if I'm wrong but there's obviously chances of false positives and negatives? This is also big business with labs charging insane amounts of money for tests.
@DeBaccoUniversity
6 ай бұрын
Check local regulations and compare when possible at what the minimal detectable levels are. Typically the lower the number the "better" the lab.