Multiplex PCR

Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.

Пікірлер: 54

  • @nafisanawal1450
    @nafisanawal1450 Жыл бұрын

    Thank you so much for the explanation.It clarified my confusions.

  • @lenkajasikova5099
    @lenkajasikova50992 жыл бұрын

    The best explanation, thank you so much!!!

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audiance

  • @danilomenezes3996
    @danilomenezes3996 Жыл бұрын

    Great explanaation! Thank you for your time.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Please contribute 1$ via super thanks. This would keep us motivated

  • @kiplimosimon1429
    @kiplimosimon14292 жыл бұрын

    The video is very informative and easy to understand. Thank you

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @jalilahmad7424
    @jalilahmad74242 жыл бұрын

    Great video To the point Thnk u so much sir

  • @mathankumar5795
    @mathankumar57952 жыл бұрын

    It is very clear explanation about multiplex, thanks

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and juniors .

  • @phdunia9498
    @phdunia94983 ай бұрын

    شكرا جزيلا لك وقد استخدمت الصور التي عرضتها في القاء محاضرتي شكرا لمساعدتك

  • @itsmybio8645
    @itsmybio86452 жыл бұрын

    Best Sir. Easily you Explained... Thanks alot

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @itsmybio8645

    @itsmybio8645

    2 жыл бұрын

    @@animatedbiologywitharpan Yes sir

  • @adamazmihan196
    @adamazmihan1968 ай бұрын

    Very Informative, I love it

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    8 ай бұрын

    Please share my channel link with your friends and help me to reach big audiance

  • @jasmintea8131
    @jasmintea81312 жыл бұрын

    Thank you ❤️

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @lisselllee6757
    @lisselllee67578 ай бұрын

    Is it that although the amplicon size must not differ too much it must still be different enough so that distinct bands may be observed during gel electrophoresis?

  • @khalidabdullah2561
    @khalidabdullah25612 жыл бұрын

    thanks for the explanation

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

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  • @Fl0wright
    @Fl0wright2 жыл бұрын

    Feels like this video could be sponsored by SpeeDx - same fundamental but totally different molecular biology

  • @moxmalamakalumbi8213
    @moxmalamakalumbi82138 ай бұрын

    Super vid. Thanks.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    8 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @tlkruthi1066
    @tlkruthi1066Ай бұрын

    Great video! 2:47 If multiplex PCR requires that the template size does not vary too much, how would it be detected separately on the agarose gel as they would be having similar molecular weights?

  • @deepikagupta4032
    @deepikagupta40322 жыл бұрын

    You are really best

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @alicechoribege3736
    @alicechoribege373610 ай бұрын

    Thank you for the clear explanation.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    10 ай бұрын

    Please share this in your college group

  • @maymunamunna6110
    @maymunamunna6110 Жыл бұрын

    thanks a lot sir.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Please share my channel link with your friends

  • @adbiologist
    @adbiologist8 ай бұрын

    Good one just like @sciencepoint

  • @ammarahchaudhary2820
    @ammarahchaudhary2820 Жыл бұрын

    Sir plzzz upload videos of primer designing with full details. How can we identify rs no, Second for RFLP and ARMS primer designing videos also

  • @riyasaha7250
    @riyasaha72502 жыл бұрын

    Sir, please make a video on allele specific PCR technique...

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Ok sure....please share my channel link with your friends and help me to reach big audience

  • @cafenoisette7299
    @cafenoisette72992 жыл бұрын

    hello, I do not understand English very well I speak French but I found your video great however there is one thing that I would like to know in the tube we put the patient's DNA only then the primers of different viruses and we look at gel electrophoresis whether it's to amplify or not. my question is at no time we add the DNA or the viral DNA viruses can you answer me please even in English I will understand thank you

  • @Fl0wright

    @Fl0wright

    2 жыл бұрын

    Il est nécessaire d'ajouter l'ADN du virus dans un autre tube et faire le même PCR pour le positive contrôle

  • @tlkruthi1066

    @tlkruthi1066

    Ай бұрын

    Viral DNA is already present in the patient's sample. No need to add it separately.

  • @qamarunnisa9538
    @qamarunnisa95382 жыл бұрын

    Always best

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @sutapapal8796
    @sutapapal87963 жыл бұрын

    So for covid we can use multiplex PCR??

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Yes multiplexing can be done

  • @teerthsunilbhave611
    @teerthsunilbhave611 Жыл бұрын

    Sir, can we use multiplex PCR for early cancer detection??

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    Yes theoretically it can be used

  • @dr.urmibhattacharjee6919
    @dr.urmibhattacharjee69197 күн бұрын

    Awesome 3 minutes . Thanks a lot. each of my samples contains one bacteria and one virus. Can I use multiplex pcr to quantify them in each sample and compare those ?

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    7 күн бұрын

    @@dr.urmibhattacharjee6919 yes you can, if the pcr product size is different lets say 400bp for bacteria specific gene and 600bp for virus specific gene then its possible .

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    7 күн бұрын

    @@dr.urmibhattacharjee6919 Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @hamidakhatun2139
    @hamidakhatun21392 жыл бұрын

    Please make a video on dna fingerprinting

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Thanks for your suggestion will make one. But i do have vodeo on southern bloting and RFLP analysis

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @hamidakhatun2139

    @hamidakhatun2139

    2 жыл бұрын

    @@animatedbiologywitharpan I am following you on both

  • @taharyasmine5944
    @taharyasmine59443 жыл бұрын

    Thank you ❤️

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @taharyasmine5944

    @taharyasmine5944

    3 жыл бұрын

    @@animatedbiologywitharpan donne