Multiplex PCR
Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.
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Thank you so much for the explanation.It clarified my confusions.
The best explanation, thank you so much!!!
@animatedbiologywitharpan
2 жыл бұрын
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Great explanaation! Thank you for your time.
@animatedbiologywitharpan
Жыл бұрын
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The video is very informative and easy to understand. Thank you
@animatedbiologywitharpan
2 жыл бұрын
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Great video To the point Thnk u so much sir
It is very clear explanation about multiplex, thanks
@animatedbiologywitharpan
2 жыл бұрын
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شكرا جزيلا لك وقد استخدمت الصور التي عرضتها في القاء محاضرتي شكرا لمساعدتك
Best Sir. Easily you Explained... Thanks alot
@animatedbiologywitharpan
2 жыл бұрын
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@itsmybio8645
2 жыл бұрын
@@animatedbiologywitharpan Yes sir
Very Informative, I love it
@animatedbiologywitharpan
8 ай бұрын
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Thank you ❤️
@animatedbiologywitharpan
2 жыл бұрын
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Is it that although the amplicon size must not differ too much it must still be different enough so that distinct bands may be observed during gel electrophoresis?
thanks for the explanation
@animatedbiologywitharpan
2 жыл бұрын
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Feels like this video could be sponsored by SpeeDx - same fundamental but totally different molecular biology
Super vid. Thanks.
@animatedbiologywitharpan
8 ай бұрын
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Great video! 2:47 If multiplex PCR requires that the template size does not vary too much, how would it be detected separately on the agarose gel as they would be having similar molecular weights?
You are really best
@animatedbiologywitharpan
2 жыл бұрын
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Thank you for the clear explanation.
@animatedbiologywitharpan
10 ай бұрын
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thanks a lot sir.
@animatedbiologywitharpan
Жыл бұрын
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Good one just like @sciencepoint
Sir plzzz upload videos of primer designing with full details. How can we identify rs no, Second for RFLP and ARMS primer designing videos also
Sir, please make a video on allele specific PCR technique...
@animatedbiologywitharpan
2 жыл бұрын
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hello, I do not understand English very well I speak French but I found your video great however there is one thing that I would like to know in the tube we put the patient's DNA only then the primers of different viruses and we look at gel electrophoresis whether it's to amplify or not. my question is at no time we add the DNA or the viral DNA viruses can you answer me please even in English I will understand thank you
@Fl0wright
2 жыл бұрын
Il est nécessaire d'ajouter l'ADN du virus dans un autre tube et faire le même PCR pour le positive contrôle
@tlkruthi1066
Ай бұрын
Viral DNA is already present in the patient's sample. No need to add it separately.
Always best
@animatedbiologywitharpan
2 жыл бұрын
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So for covid we can use multiplex PCR??
@animatedbiologywitharpan
3 жыл бұрын
Yes multiplexing can be done
Sir, can we use multiplex PCR for early cancer detection??
@animatedbiologywitharpan
Жыл бұрын
Yes theoretically it can be used
Awesome 3 minutes . Thanks a lot. each of my samples contains one bacteria and one virus. Can I use multiplex pcr to quantify them in each sample and compare those ?
@animatedbiologywitharpan
7 күн бұрын
@@dr.urmibhattacharjee6919 yes you can, if the pcr product size is different lets say 400bp for bacteria specific gene and 600bp for virus specific gene then its possible .
@animatedbiologywitharpan
7 күн бұрын
@@dr.urmibhattacharjee6919 Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Please make a video on dna fingerprinting
@animatedbiologywitharpan
2 жыл бұрын
Thanks for your suggestion will make one. But i do have vodeo on southern bloting and RFLP analysis
@animatedbiologywitharpan
2 жыл бұрын
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@hamidakhatun2139
2 жыл бұрын
@@animatedbiologywitharpan I am following you on both
Thank you ❤️
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3 жыл бұрын
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@taharyasmine5944
3 жыл бұрын
@@animatedbiologywitharpan donne