Welcome to *ClevaLab* - if you like the video, please give it a 👍and subscribe for more videos. Also, if you have any questions, feel free to ask in the comments. 🤓

Thank you so much, you can't imagine how much you have helped me. I have been confused for more an year and ths cleared everything

Loved it.. Perfect description of old methods to the new ones. Videos like this are much needed to understand these concepts visually.

@ClevaLab

4 ай бұрын

I'm glad you liked it and found it helpful. 🤓 Thanks for taking the time to comment.

The animations used in this video are very helpful and well done. Excellent overview of Sanger sequencing.

@ClevaLab

Жыл бұрын

Thanks for your comment. 🤓 I'm so glad you liked it.

Compared to so many materials, this one really made me understand it . Thanks a lot.

@ClevaLab

4 ай бұрын

I'm glad you liked it. 🤓 Thanks for your comment.

❤this is so good, composed & clear... Thanks

@ClevaLab

10 ай бұрын

Thanks for taking the time to comment. 🤓 I'm glad you enjoyed the video.

Very deatiled and informative video with excellent visuals, thank you!!!

@ClevaLab

11 ай бұрын

Thanks so much! I'm glad you liked it. 🤓

Your Videos are so helpful 😊.Thanks a lot ❤

@ClevaLab

11 ай бұрын

Thanks for taking the time to comment. 🤓 I'm glad you're enjoying the videos.

thabk you so much! This is so clear!

@ClevaLab

11 ай бұрын

Thank you for watching. 🤓 I'm glad you liked it. 👍

This is brilliant. Thank you so much

@ClevaLab

11 ай бұрын

Thanks for taking the time to comment. 🤓 I'm glad you're enjoying the videos.

Wow! You explained so well. It's easy to understand. You should be a good teacher. Thank you

@ClevaLab

5 ай бұрын

I'm glad you found it useful. 🤓 Thanks for taking the time to comment.

Amazing video :D thank you very much 💛

@ClevaLab

8 ай бұрын

I'm glad you liked it. Thanks for taking the time to comment. 🤓

Hey.. your videos are great. would love to see more of your videos. I am not finding many here. Please upload more videos. May be on fourth-generation technologies ;)

@ClevaLab

8 ай бұрын

Thanks for your comment. 🤓 Ah yes, I'm working on more! Yes, I've got PacBio and Nanopore on my list. 👍

This is one of the best videos I have seen in biotech so far.

@ClevaLab

11 ай бұрын

Thanks so much! That's great to hear. I'm glad you enjoyed the video. 🤓

wow, just a fantastic video thank you.🥰

@ClevaLab

9 ай бұрын

Thanks for your comment. 🤓 I'm so glad it helped you.

Great video!

@ClevaLab

7 ай бұрын

Thanks for your comment. 🤓 I'm glad it helped.

Can't thank you enough!!!❤

@ClevaLab

28 күн бұрын

You're welcome. 🤓

I studied Microbiology at university from '93-'98 and this was all a black box. I appreciate the depth of this exploitation. Thank you!

@ClevaLab

5 ай бұрын

Thanks for your comment. 🤓 I'm glad you liked the video.

Best and detailed video

@ClevaLab

Ай бұрын

Thanks for your comment. 🤓 I'm glad you liked it.

Amazing video, I got to know about many things yhrough this. I am currently trying to get to know about how to write research paper and for that I chose a topic of next generation sequencing can you provide me a pdf for that purpose?

Excelent video. I just had a doubt about why exactly the radiolabel was added to the dATP. In other materials it says that the radioactive or fluorescent label is added to the ddNTPs, which makes more sense to me. But, since the dATP could be added at a random position in the chain, why did Dr. Sanger do it?

Hello, thanks for the explanation it was very very clear. Thank you also for a previous response explaining why we can be certain to get every nucleotide sequenced during the first iteration. But i just had one question about the comparison between Sanger sequencing and NGS, when you talked about sensitivity to detect a base within a background of other DNA, i didn't understand if it was a bad thing or not. Only* 15-20% implied that greater should be better or am i incorrect ?

plz upload more molecular biology videos.thanks

@ClevaLab

7 ай бұрын

Thanks for your comment. 🤓 I hope to do so soon!

you make great videos, you should do one on western immunoblotting

@ClevaLab

10 ай бұрын

Thanks for your comment. 🤓 I'm glad you're enjoying the videos. Good idea, I'll put that one on my list. 👍

Very impressive animation. @3:16 dNTPs are not radiolabeled instead ddNTPs are radiolabeled.

@ClevaLab

8 ай бұрын

Thanks for your comment. 🤓 I'm glad you liked the animation. In the original article by Sanger _et al._ the *dATPs* were radiolabeled, see the article here: www.pnas.org/doi/abs/10.1073/pnas.74.12.5463

thank you for this amazing video and for providing a pdf file with it. just I have a question, as the incorporation of ddNTPs occur RANDOMLY, how can we make sure , that each position of Adenine nucleotide for example along the template will be attached to a ddTTP once??? I mean, what if this nucleotide always with each cycle attached to a normal dTTP , this means that this nucleotide won't be read , right?

@ClevaLab

10 ай бұрын

Thanks for your comment. 🤓 In the video, I did oversimplify things. For simplicity, only one strand of DNA was illustrated. However, the usual input amount for Sanger sequencing for human genomic DNA is around 200 ng. In 200 ng of human genomic DNA, there are almost 60,000 copies of the genome. This means that multiple labelled fragments are generated for each base in the sequence. The higher the input of DNA (and hence copies), the higher the final signal will be for that base in the sequencing result. There will be differences in signal intensity on the sequencing trace (chromatogram) due to the slightly different amounts of fragments at each base position. That’s why the heights of the peaks are somewhat different. I hope this makes sense. Please let me know if you have any further questions.

@almasrialoo9924

9 ай бұрын

@@ClevaLab yes I get it . thank you so much for this amazing explanation.😊

might be the first time i hear it referred to as the oxygen, instead of the hydroxide

i don't understand why there is only one primer in Sanger sequencing TT

Thank you so much, you can't imagine how much you have helped me. I have been confused for more an year and ths cleared everything

@ClevaLab

3 ай бұрын

Thanks for your comment. 🤓 I'm so glad it was helpful.