Next Generation Sequencing 1: Overview - Eric Chow (UCSF)

Ғылым және технология

www.ibiology.org/techniques/n...
Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples.
In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and cost $3 billion. Current next generation sequencing methods allow a human genome to be sequenced for $1000, in 48 hours! In this talk, Eric Chow explains the chemistry behind next generation sequencing, and describes how the next gen sequencers detect and display results. The most commonly used Illumina sequencers are image based and detect the addition of fluorescently labelled nucleotides. Chow also describes two different next generation sequencing technologies which provide benefits such as much longer reads but with downsides such as higher error rates. Chow finishes the talk with some insights into medical applications of next gen sequencing such as much less invasive prenatal testing or cancer detection.
In two short how-to videos, Chow gives advice on purifying DNA samples using magnetic beads and on determining the quality of your nucleic acid sample using an Agilent Bioanalyzer.
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.

Пікірлер: 209

  • @hansvanlennep6376
    @hansvanlennep63764 жыл бұрын

    Finally! Someone that clearly explains the basics of NGS in a understandable way. Thank you very much!

  • @rehabalsaleh166

    @rehabalsaleh166

    3 жыл бұрын

    Exactly!

  • @hamidkiangaikani

    @hamidkiangaikani

    2 жыл бұрын

    Yeah, really!

  • @kitony

    @kitony

    2 жыл бұрын

    Nice one for sure

  • @anonviewerciv
    @anonviewerciv3 жыл бұрын

    7:47 Illumina. 21:00 Nanopore. 23:23 Pacific Bioscience.

  • @yibincao7924
    @yibincao79245 жыл бұрын

    Dear Eric, fantastic NGS sequencing intro! Thank you!

  • @wenningqin4210
    @wenningqin42104 жыл бұрын

    Thank you for making NGS so easy to understand! Really appreciate your taking time to make this video and help!

  • @Lenz979
    @Lenz9795 жыл бұрын

    Eric Chow! Great video. Never seen anyone explaining NGS so well as you do. Impressive. Thank you for your efforts! Please keep on making these videos!

  • @donthompson1517
    @donthompson15173 жыл бұрын

    Thank you Dr Chow! Your explanations are very clear.

  • @robelbelay4065
    @robelbelay40655 жыл бұрын

    Absolutely fantastic presentation! Very clear and concise. Thank you

  • @SnappyScience
    @SnappyScience Жыл бұрын

    This was exactly what I needed. A clear explanation of NGS, how it works and why it's important. Thank you!

  • @flaviorump607
    @flaviorump6073 жыл бұрын

    Thank you, Eric and iBiology team for a very well-produced and thorough video. My exam prep just became more fun :)

  • @carlosp.e.bedson4011
    @carlosp.e.bedson40113 жыл бұрын

    An amazingly professional and clear presentation. Thank you!

  • @jixingliu9501
    @jixingliu95017 ай бұрын

    Great video! I have messed up details of NGS for many years until seeing this video.

  • @danielzarate64
    @danielzarate64 Жыл бұрын

    Thank you!!! That was by far the best breakdown of how NGS works!

  • @journeymansix
    @journeymansix2 жыл бұрын

    This is the most lucid explanation of next gen sequencing I've seen. Thanks, Eric!

  • @marchielumsy1673
    @marchielumsy16732 жыл бұрын

    This is such a clear explanation of how these sequencing technologies work. I was focused the whole time. Great work!

  • @antonioaugustolimateixeirajuni
    @antonioaugustolimateixeirajuni7 ай бұрын

    I loved this video and how you clearly explained all the basic concepts about NGS

  • @tm67245
    @tm672453 жыл бұрын

    Clear and To the point explanation! thank you Dr. Chow

  • @ThePoptartjunkie
    @ThePoptartjunkie4 жыл бұрын

    Fantastic presentation. Its always great to see someone keeping it simple, but simplicity goes a long way. You have educated so many people!

  • @skippyleif
    @skippyleif Жыл бұрын

    Excellent video! I love how concise and easy to understand this is. I am starting to analyze NGS data and this just made things much easier.

  • @bronaghmcdonnell8644
    @bronaghmcdonnell8644 Жыл бұрын

    Absolutely brilliant 🎉 I'll be watching all of prof Chows videos. The reference to suppliers and technologies was excellent, the historical context of Sanger Sequencing was really valuable to then appreciate newer methods, and the videos of the pieces of equipment used was so so so helpful to visualise how this happens in a lab, or on the moon. You covered alot with perfect explanation and delivery. Thank you. I've just started working in a genomics lab and this has helped me alot.

  • @francescalittle
    @francescalittle4 жыл бұрын

    Thanks to you, I FINALLY understand this topic! You explained it so well.

  • @rahulboh
    @rahulboh4 жыл бұрын

    So comprehensive and simple to understand, what a great resource. Thank you and Kudos Dr. Chow!

  • @Anetpersa
    @Anetpersa4 жыл бұрын

    I fell in love with molecular biology and chemistry again 😍 Thank you for this amazing video.

  • @PelleSenior
    @PelleSenior2 жыл бұрын

    Nice job. This was very understandable, yet thorough enough to get a sense for the caveats of different methods.

  • @SZM396
    @SZM39611 ай бұрын

    Highly recommended to watch. very clear and detailed. thank you for your efforts.

  • @audairwashdeh9740
    @audairwashdeh97402 жыл бұрын

    Thank you so much for simplifying the mystery of NGS! A very helpful video.

  • @user-gr4fh9el3y
    @user-gr4fh9el3y2 жыл бұрын

    Your presentation is MARVELOUS! Thank you so much for explaining it simple enough to allow me to walk through it with my high school knowledge!

  • @mayureshvishwanathan6458
    @mayureshvishwanathan6458 Жыл бұрын

    I was hunting for a start to understand basic Next gen sequencing in a easy way and finally got to watch this video. Thank you very much

  • @sagek7949
    @sagek79492 жыл бұрын

    This was so very helpful. Thank you so much! This is by far the best explanation on NGS!

  • @zhenli2293
    @zhenli22934 жыл бұрын

    This is great! Professional explanation and smooth and logical flow, which makes it very easy to understand.

  • @izj-19
    @izj-192 жыл бұрын

    This was so clearly explained. Review of basics was really helpful. Thank you!

  • @liamnicholas97
    @liamnicholas978 ай бұрын

    What an excellent lecture -Thank you Professor.

  • @sdsa007
    @sdsa007 Жыл бұрын

    Thanks ... I know I watched this before... but its so clear and basic that I watched it again! Thanks for the refresh!

  • @wanderingmanz
    @wanderingmanz2 жыл бұрын

    Very well explained. Truely professional and takes along the students of all background knowledge.

  • @Science.Medicine209
    @Science.Medicine2095 жыл бұрын

    Excellent and comprehensive presentation ..Thank you Doctor...Kindly Do more video like that specially on its Analysis

  • @fdboucher
    @fdboucher4 жыл бұрын

    Brilliant presentation! THANK YOU Eric!

  • @AsfandyarAfridi
    @AsfandyarAfridi Жыл бұрын

    GOAT video on sequencing

  • @mitalipandey3799
    @mitalipandey37993 жыл бұрын

    This is a very well thought through and presented video. All the concepts have been researched and presented in a very palatable manner with no frills (background music).The speaker also has very clear diction and doesn't make any unnecessary movements. This allows the viewer to actually see what is being referred to instead of the speaker covering it up. Please keep making and posting these videos - they are very well made and informative. All the best.

  • @harshitashrivastava8877
    @harshitashrivastava88772 жыл бұрын

    Best video on Sequencing till date. Thank you 💯

  • @inshaishrat4371
    @inshaishrat43716 ай бұрын

    The best video on NGS ever!

  • @melix196
    @melix1963 жыл бұрын

    No textbook nor lecturer explained this better. Thank you so much for this high quality video and the recordings from the lab made this video extra interesting ☺️

  • @lbgrindel
    @lbgrindel Жыл бұрын

    Great video. I liked the explanation of alternatives to Illumina. Never knew they existed.

  • @natalyabeschastnova4049
    @natalyabeschastnova40494 жыл бұрын

    amazing video! very detailed and clearly explained! thank you for making this video and posting it!

  • @user-ko9rj8yg4z
    @user-ko9rj8yg4z3 жыл бұрын

    Such a neat clear and crisp explanation

  • @wynnewijaya158
    @wynnewijaya1583 ай бұрын

    Thank you so much for this, Eric! Very easy to understand.

  • @jishasureshkumar2466
    @jishasureshkumar2466 Жыл бұрын

    Loved this presentation! Clearly explained.

  • @snowballandbubbles9379
    @snowballandbubbles93793 жыл бұрын

    The Best lecture on NGS! Excellent!

  • @razanalrawahi2291
    @razanalrawahi22913 жыл бұрын

    Omgg you just saved my grade!! thank you so much for this amazing work!

  • @alexandrahiller8223
    @alexandrahiller82233 жыл бұрын

    Great presentation. Clear and nicely explained. Thank you!

  • @exciton007
    @exciton0072 ай бұрын

    Thanks for this great presentation!

  • @kareemjeiroudi1964
    @kareemjeiroudi19644 жыл бұрын

    Thanks for this briefly well-put summary on NGS

  • @pek972
    @pek9722 жыл бұрын

    Excellent! To make so clear something that complex! Thank you very much.

  • @yeeguan99
    @yeeguan99 Жыл бұрын

    Thank you for the clear and consice lecture on NGS!

  • @abutaleb3622
    @abutaleb36222 жыл бұрын

    Just precious way of teaching

  • @franciscoagus
    @franciscoagus3 жыл бұрын

    Congrats mate. You have some serious teaching skills. Quite complicated stuff explained very easy and simple.

  • @juancarlostantalean6496
    @juancarlostantalean64963 жыл бұрын

    Thank you very much Eric !!. I am professor and it is so good for be used in class of Molecular Biology about NGS sequencing. Best regards from Ica-Perú, the place of Nazca Lines !!

  • @laworder5158
    @laworder51583 жыл бұрын

    Exciting future for NGS. Thanks for this great video.

  • @rzipper1716
    @rzipper17163 жыл бұрын

    This IS the best review of NGS ! Thanks !!

  • @MrViermeRau
    @MrViermeRau5 жыл бұрын

    Really good video, it made a lot of things clearer, thanks

  • @delprice3007
    @delprice30074 жыл бұрын

    Outstanding presentation, many thanks.

  • @Kat-lx7qq
    @Kat-lx7qq Жыл бұрын

    wow! this was fantastic thank you so much Eric

  • @dr.janmejaykpandey4802
    @dr.janmejaykpandey48024 жыл бұрын

    Awesome (Very Comprehensible). Your Video helped me a great deal in my class on DNA sequencing.

  • @ScottRobertsPage
    @ScottRobertsPage5 жыл бұрын

    Very informative video. This helped me understand how sequencing works much better than the videos from Illumina.

  • @dangthatscool1
    @dangthatscool12 жыл бұрын

    Great overview! Thank you.

  • @mly2883
    @mly28832 жыл бұрын

    Thank you Eric, well explained.

  • @nikolayanachkov4553
    @nikolayanachkov45532 жыл бұрын

    This was such a great video ! Thank you !

  • @matiwosaraya514
    @matiwosaraya5144 жыл бұрын

    Great video, Thanks for your time.

  • @Rodrigo-yx3sn
    @Rodrigo-yx3sn3 жыл бұрын

    Really good explanation and comparison between technologies.

  • @Iqertwyegcje
    @Iqertwyegcje2 жыл бұрын

    Thank you, it helps me a lot to understand sequencing process

  • @katelinklyn8335
    @katelinklyn83352 жыл бұрын

    Very interesting and easy to understand. Thank you!

  • @nguyetnhiau1
    @nguyetnhiau13 жыл бұрын

    Great presentation! Thank you so much!

  • @angelapotemkina_rozenfeld7137
    @angelapotemkina_rozenfeld71374 жыл бұрын

    Thank you very much! This is very clear and understandable explanation of NGS

  • @patrickpetersen9887
    @patrickpetersen98873 жыл бұрын

    Very clear and informative...thank you!

  • @hesnayigit8840
    @hesnayigit88402 жыл бұрын

    Thank you very much, this is a great presentation of the systems.

  • @sangeethasaji1
    @sangeethasaji14 жыл бұрын

    Its a fantastic explanation. Great work

  • @hangpham7588
    @hangpham75883 жыл бұрын

    Thank you so much! This's all I need

  • @andrewrobinson5581
    @andrewrobinson55813 жыл бұрын

    Thank you so much this presentation was perfect!

  • @justg.5874
    @justg.58743 жыл бұрын

    Thank you. Used this to prepare for a job interview💛.

  • @songthanh896
    @songthanh896 Жыл бұрын

    Thank you very much for your clear explanation!

  • @sohamparikh3085
    @sohamparikh30853 жыл бұрын

    Very easy to understand, thank you!

  • @shih-yihsiung239
    @shih-yihsiung2392 жыл бұрын

    Thanks for uploading this nice video.

  • @rinm2564
    @rinm25642 жыл бұрын

    Thank you very much. It is the best explanation! Good luck in your work.

  • @mayeninim6281
    @mayeninim62812 жыл бұрын

    This is great! Thank you!

  • @madhusudanm5835
    @madhusudanm58353 жыл бұрын

    great and very informative video presentation. amazing explanation.

  • @karlalima6497
    @karlalima6497 Жыл бұрын

    Thanks you so much for this!

  • @eliabd7726
    @eliabd77262 жыл бұрын

    I appreciate for all of your videos. Please make more videos.

  • @CodeJeffo
    @CodeJeffo2 жыл бұрын

    Excellent explanations. Thank you.

  • @mervatel-deftar8412
    @mervatel-deftar8412Ай бұрын

    Amazing overview Excellent

  • @psculpta
    @psculpta4 жыл бұрын

    Excellent job! I wish I could take a class from this professor.

  • @kertamo6721
    @kertamo67212 жыл бұрын

    Truly a great presentation. Thank you :)

  • @juantrinidad853
    @juantrinidad8533 жыл бұрын

    Great video! Very clear. Love it

  • @FufuFang
    @FufuFang4 жыл бұрын

    Thank you so much!!! This is really informative!

  • @manoappapillai
    @manoappapillai3 жыл бұрын

    Super . . very clear presentation. Please do more. Thank you.

  • @oliverhurni9204
    @oliverhurni92043 жыл бұрын

    Thank you for this video!

  • @aqsasaeed5237
    @aqsasaeed52374 жыл бұрын

    Excellent Explanation of the topic

  • @qioqio5662
    @qioqio56624 жыл бұрын

    Thank you, very clear and understandable!

  • @ekimkara9260
    @ekimkara92603 жыл бұрын

    Hi, thank you for this review!

  • @alimughal715
    @alimughal7154 жыл бұрын

    Realy very nice and understandable presentation.

  • @nicolaeionescu-kosa132
    @nicolaeionescu-kosa1322 жыл бұрын

    Excellent presentation, could understand more things. Thank you

  • @sawses
    @sawses4 жыл бұрын

    Thanks for this! I'm a recent bio grad and wanted to figure out how NGS differs from Sanger. Well done.

  • @Stephanbitterwolf
    @Stephanbitterwolf4 жыл бұрын

    Thank you for this great lecture

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