Hydrophobic interaction chromatography ( HIC) | Application of HIC

Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.

Пікірлер: 69

  • @qamaral-ubiedi5404
    @qamaral-ubiedi54043 жыл бұрын

    Do you know how long should my HIC column be to isolate GFP from 1g e.Coli?

  • @qetsiyah1766
    @qetsiyah17664 ай бұрын

    The visuals are great! They were so helpful for my understanding. Thank you.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @JJH2004
    @JJH20044 ай бұрын

    So basically the hydrophobic analytes take longer to be detected as they interact more with the hydrophobic column?

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 ай бұрын

    Yes the elution time for hydrophobic molecules are more

  • @sh7arwt3teer99
    @sh7arwt3teer992 жыл бұрын

    please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

  • @blueyolker4961

    @blueyolker4961

    2 жыл бұрын

    i forgot how it worked but i think it had to do with ammonium (NH4) being a keypart of proteins

  • @valentinascatizzi6406
    @valentinascatizzi64063 жыл бұрын

    Clear and fluid explanation, thank you . greets

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

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  • @rukhsarfatima2283
    @rukhsarfatima22837 ай бұрын

    How can water molecules mask the hydrophobic surfaces???

  • @vg2358
    @vg23588 ай бұрын

    Great explanation, thank you very much!

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    8 ай бұрын

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    8 ай бұрын

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  • @SS-lr6uk
    @SS-lr6uk4 жыл бұрын

    Thank you sir it was much needed.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Please share among friends and help me to reach big audience

  • @suvismaya5980
    @suvismaya59803 жыл бұрын

    Thank u so much sir I had problem in understanding hw they will use HIC for virus purification...now it's clear......😊😊😊😊

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    @animatedbiologywitharpan

    3 жыл бұрын

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  • @suvismaya5980

    @suvismaya5980

    3 жыл бұрын

    @@animatedbiologywitharpan sure😊

  • @shivaniathani8082
    @shivaniathani80823 жыл бұрын

    All the videos on this channel are very very useful. Thank you.

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    3 жыл бұрын

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  • @Nick.5.9..175
    @Nick.5.9..1752 ай бұрын

    You are amazing! Thank you so much

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @maninderdhillon8351
    @maninderdhillon83513 жыл бұрын

    Excellent.. presentation .

  • @animatedbiologywitharpan

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    3 жыл бұрын

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  • @maninderdhillon8351

    @maninderdhillon8351

    3 жыл бұрын

    Sure.. I will do it.. but it will inevitably reach out to millions one day... Keep the good work going..

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    support from audience is my motivation....hope to bring nice content soon

  • @himankjain5473
    @himankjain54734 жыл бұрын

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    @animatedbiologywitharpan

    4 жыл бұрын

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    @animatedbiologywitharpan

    4 жыл бұрын

    Please subscribe to my other channel kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html

  • @nareshbarik5384
    @nareshbarik53842 ай бұрын

    Thank you ❤

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 ай бұрын

    could you please help me by sharing my contents with your friends

  • @bioline8441
    @bioline84413 жыл бұрын

    so good.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Please share my channel link with your friends

  • @davidrosen5137
    @davidrosen5137 Жыл бұрын

    Super helpful.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    Жыл бұрын

    In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @srijandey87
    @srijandey874 ай бұрын

    Very well explained ❤

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 ай бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @shrutikachaudhary672
    @shrutikachaudhary6723 жыл бұрын

    Little bit clarity is needed while speaking rest the video was really nice and explanation through diagrams is good.... Keep it up 👍

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    Thanks for the feedback....these constructive criticisms would help to improve the quality in future

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    @animatedbiologywitharpan

    3 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @user-rw6fe3vm5b
    @user-rw6fe3vm5b6 ай бұрын

    great

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    6 ай бұрын

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  • @songohan393
    @songohan3932 жыл бұрын

    Thannnk you

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @tamanaaswal5316
    @tamanaaswal53162 жыл бұрын

    Moj krdi sir mst explanation

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

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  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Don't forget to check out all my playlist

  • @aparnaaa273
    @aparnaaa2734 жыл бұрын

    Need to increase volume a bit . Thanks for the vdo

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Please share among your friends and help me to reach big audience

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    @animatedbiologywitharpan

    4 жыл бұрын

    Please subscribe to my other channel kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html

  • @KentJustinBanguilan
    @KentJustinBanguilan20 күн бұрын

    You are so smart :)

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    20 күн бұрын

    Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @sasukeuhiha1131
    @sasukeuhiha11312 жыл бұрын

    I used to have a problem understanding HIC but watching this video has made me realize that I also have a problem understanding English.

  • @rashmiranjansahoo3975
    @rashmiranjansahoo39754 жыл бұрын

    Explanation could have been more better with written texts.

  • @HectixShorts
    @HectixShorts2 жыл бұрын

    Another nice effort!

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    Please share my channel link with your friends and help me to reach big audience

  • @nanhelalgupta476
    @nanhelalgupta4764 жыл бұрын

    please does better in which don't mine time limitation..

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Thanks for the suggestion

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Please subscribe to my other channel it's in hindi kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html

  • @richajoshi2578
    @richajoshi25784 жыл бұрын

    Nice but plzz make videos on animal and plant biotechnology important topics

  • @hammadak8
    @hammadak84 жыл бұрын

    i think u have problem in your voice... kindly focus on it. and content is good but only voice.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    4 жыл бұрын

    Ya my mic was not working properly

  • @hammadak8

    @hammadak8

    4 жыл бұрын

    @@animatedbiologywitharpan its ok bro...keep it up

  • @_CatalystGuy
    @_CatalystGuy9 ай бұрын

    Please speak a little bit loud😢

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    9 ай бұрын

    Yes , in this video the audio is not good. May be I should make a new video

  • @kuldeepbiotech4014
    @kuldeepbiotech40143 жыл бұрын

    Such a low pitch sound really irritating and such things makes the learning distracted. Audio quality always matters when we try to maintain concentration.

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    3 жыл бұрын

    I took your comment positively....the sound quality would be improved in future

  • @worldshootout3298
    @worldshootout32982 жыл бұрын

    If possible plz tell hindi 🤒

  • @animatedbiologywitharpan

    @animatedbiologywitharpan

    2 жыл бұрын

    thanks for your suggestion.. I will make a video in hindi as well