Hydrophobic interaction chromatography ( HIC) | Application of HIC
Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.
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Do you know how long should my HIC column be to isolate GFP from 1g e.Coli?
The visuals are great! They were so helpful for my understanding. Thank you.
@animatedbiologywitharpan
4 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
So basically the hydrophobic analytes take longer to be detected as they interact more with the hydrophobic column?
@animatedbiologywitharpan
4 ай бұрын
Yes the elution time for hydrophobic molecules are more
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
@blueyolker4961
2 жыл бұрын
i forgot how it worked but i think it had to do with ammonium (NH4) being a keypart of proteins
Clear and fluid explanation, thank you . greets
@animatedbiologywitharpan
3 жыл бұрын
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How can water molecules mask the hydrophobic surfaces???
Great explanation, thank you very much!
@animatedbiologywitharpan
8 ай бұрын
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@animatedbiologywitharpan
8 ай бұрын
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Thank you sir it was much needed.
@animatedbiologywitharpan
4 жыл бұрын
Please share among friends and help me to reach big audience
Thank u so much sir I had problem in understanding hw they will use HIC for virus purification...now it's clear......😊😊😊😊
@animatedbiologywitharpan
3 жыл бұрын
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@suvismaya5980
3 жыл бұрын
@@animatedbiologywitharpan sure😊
All the videos on this channel are very very useful. Thank you.
@animatedbiologywitharpan
3 жыл бұрын
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You are amazing! Thank you so much
@animatedbiologywitharpan
2 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Excellent.. presentation .
@animatedbiologywitharpan
3 жыл бұрын
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@maninderdhillon8351
3 жыл бұрын
Sure.. I will do it.. but it will inevitably reach out to millions one day... Keep the good work going..
@animatedbiologywitharpan
3 жыл бұрын
support from audience is my motivation....hope to bring nice content soon
Sir if i purchase your course then will i be able to get paid videos of other teachers of iit jam
@animatedbiologywitharpan
4 жыл бұрын
Yup my friend you would be able to attain all the lectures from all the educators... do subscribe and use my code AP10
@animatedbiologywitharpan
4 жыл бұрын
Please subscribe to my other channel kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html
Thank you ❤
@animatedbiologywitharpan
2 ай бұрын
could you please help me by sharing my contents with your friends
so good.
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends
Super helpful.
@animatedbiologywitharpan
Жыл бұрын
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Very well explained ❤
@animatedbiologywitharpan
4 ай бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Little bit clarity is needed while speaking rest the video was really nice and explanation through diagrams is good.... Keep it up 👍
@animatedbiologywitharpan
3 жыл бұрын
Thanks for the feedback....these constructive criticisms would help to improve the quality in future
@animatedbiologywitharpan
3 жыл бұрын
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great
@animatedbiologywitharpan
6 ай бұрын
Please share my channel link with your friends and help me to reach big audiance
Thannnk you
@animatedbiologywitharpan
2 жыл бұрын
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Moj krdi sir mst explanation
@animatedbiologywitharpan
2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@animatedbiologywitharpan
2 жыл бұрын
Don't forget to check out all my playlist
Need to increase volume a bit . Thanks for the vdo
@animatedbiologywitharpan
4 жыл бұрын
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@animatedbiologywitharpan
4 жыл бұрын
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You are so smart :)
@animatedbiologywitharpan
20 күн бұрын
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
I used to have a problem understanding HIC but watching this video has made me realize that I also have a problem understanding English.
Explanation could have been more better with written texts.
Another nice effort!
@animatedbiologywitharpan
2 жыл бұрын
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please does better in which don't mine time limitation..
@animatedbiologywitharpan
4 жыл бұрын
Thanks for the suggestion
@animatedbiologywitharpan
4 жыл бұрын
Please subscribe to my other channel it's in hindi kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html
Nice but plzz make videos on animal and plant biotechnology important topics
i think u have problem in your voice... kindly focus on it. and content is good but only voice.
@animatedbiologywitharpan
4 жыл бұрын
Ya my mic was not working properly
@hammadak8
4 жыл бұрын
@@animatedbiologywitharpan its ok bro...keep it up
Please speak a little bit loud😢
@animatedbiologywitharpan
9 ай бұрын
Yes , in this video the audio is not good. May be I should make a new video
Such a low pitch sound really irritating and such things makes the learning distracted. Audio quality always matters when we try to maintain concentration.
@animatedbiologywitharpan
3 жыл бұрын
I took your comment positively....the sound quality would be improved in future
If possible plz tell hindi 🤒
@animatedbiologywitharpan
2 жыл бұрын
thanks for your suggestion.. I will make a video in hindi as well