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Gram Staining Procedure Animation Microbiology - Principle, Procedure, Interpretation

📌 𝐅𝐨𝐥𝐥𝐨𝐰 𝐨𝐧 𝐈𝐧𝐬𝐭𝐚𝐠𝐫𝐚𝐦:- / drgbhanuprakash
📌𝗝𝗼𝗶𝗻 𝗢𝘂𝗿 𝗧𝗲𝗹𝗲𝗴𝗿𝗮𝗺 𝗖𝗵𝗮𝗻𝗻𝗲𝗹 𝗛𝗲𝗿𝗲:- t.me/bhanuprak...
📌𝗦𝘂𝗯𝘀𝗰𝗿𝗶𝗯𝗲 𝗧𝗼 𝗠𝘆 𝗠𝗮𝗶𝗹𝗶𝗻𝗴 𝗟𝗶𝘀𝘁:- linktr.ee/DrGB...
Gram staining procedure animation - Principle, Procedure, Interpretation
Principle:
--------------
Staining is an auxiliary technique used in microscopic techniques used to enhance the clarity of the microscopic image. Stains and dyes are widely used in the scientific field to highlight the structure of biological specimens, cells, tissues etc.
The most widely used staining procedure in microbiology is the Gram stain, discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Gram-negative bacteria are decolorized by the alcohol, losing the color of the primary stain, purple. Gram-positive bacteria are not decolorized by alcohol and will remain as purple. After decolorization step, a counterstain is used to impart a pink color to the decolorized gram-negative organisms.
Importance of a Gram Stain:
---------------------------------------------
The Gram stain is a very important preliminary step in the initial characterization and classification of bacteria. It is also a key procedure in the identification of bacteria based on staining characteristics, enabling the bacteria to be examined using a light microscope. The bacteria present in an unstained smear are invisible when viewed using a light microscope. Once stained, the morphology and arrangement of the bacteria may be observed as well. Furthermore, it is also an important step in the screening of infectious agents in clinical specimens such as direct smears from a patient.
The Gram stain procedure enables bacteria to retain color of the stains, based on the differences in the chemical and physical properties of the cell wall.
1. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall. Example: Staphylococcus aureus
2. Gram negative bacteria: Stain red or pink due to retaining the counter staining dye called Safranin.
Example: Escherichia coli
#gramstainingprocedure #microbiology #usmlevideos #usmle #usmlestep1videos #neetpg #fmge #mbbs #microbiologyvideos #microlectures #animatedmedicalvideos #animatedmicrobiologyvideos #drgbhanuprakash

Пікірлер: 192

  • @doctorbhanuprakash
    @doctorbhanuprakash Жыл бұрын

    📌 𝐅𝐨𝐥𝐥𝐨𝐰 𝐨𝐧 𝐈𝐧𝐬𝐭𝐚𝐠𝐫𝐚𝐦:- instagram.com/drgbhanuprakash 📌𝗝𝗼𝗶𝗻 𝗢𝘂𝗿 𝗧𝗲𝗹𝗲𝗴𝗿𝗮𝗺 𝗖𝗵𝗮𝗻𝗻𝗲𝗹 𝗛𝗲𝗿𝗲:- t.me/bhanuprakashdr 📌𝗦𝘂𝗯𝘀𝗰𝗿𝗶𝗯𝗲 𝗧𝗼 𝗠𝘆 𝗠𝗮𝗶𝗹𝗶𝗻𝗴 𝗟𝗶𝘀𝘁:- linktr.ee/DrGBhanuprakash

  • @AaryaGhuge
    @AaryaGhuge3 жыл бұрын

    This just explained it to me in 4 mins what my teacher couldn’t do in an hour! Thanks a lot 😇

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    You're very welcome!

  • @rose_-nb7nk

    @rose_-nb7nk

    Жыл бұрын

    😂😂😂😂😂

  • @Juliee1__

    @Juliee1__

    Жыл бұрын

    True'😶😂

  • @educationlobby985

    @educationlobby985

    Жыл бұрын

    Mine teachers couldn't understand it in a year 😂

  • @ajcreations2486

    @ajcreations2486

    Жыл бұрын

    True 🤣

  • @doctorbhanuprakash
    @doctorbhanuprakash Жыл бұрын

    Hello Everyone, JOIN MY TELEGRAM GROUP HERE:- t.me/bhanuprakashdr Subscribe To My Mailing List:- linktr.ee/DrGBhanuprakash

  • @doctorbhanuprakash
    @doctorbhanuprakash4 жыл бұрын

    Medvizz Animated medical video lectures : 1200+ complete animated medical Video lectures includes all high-yield topics enough to cover all contents of mbbs and usmle step 1 . Subjects covered are - Anatomy , Physiology , Biochemistry , Pathology , Pharmacology , Genetics , Immunology , Microbiology , Histology , Embryology Clinical cases with detailed explanations for relevant topics High-yield notes for all above subjects Question bank wich covers all aspects of NEETPG , USMLE and PLAB software of Question bank mimic actual exam experience of respective licensing exams www.medvizz.com +91 9885588972 ( whatsapp )

  • @lokeshaalone6550

    @lokeshaalone6550

    4 жыл бұрын

    Some video not show why

  • @ePlantHealth

    @ePlantHealth

    3 жыл бұрын

    Please, could you suggest me the software you are using to creat such animation videos.

  • @aftabahmedsoomro9141

    @aftabahmedsoomro9141

    3 жыл бұрын

    .

  • @shreyadutta9896
    @shreyadutta98964 жыл бұрын

    I have seen many videos but this one is the best

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    thank you

  • @TanmayBhoyar-zb8sl
    @TanmayBhoyar-zb8sl2 ай бұрын

    Legend watching in 2024😂😂😂😂😂

  • @suryaprasantnaram5141
    @suryaprasantnaram5141 Жыл бұрын

    Sir Your explaining topic vry clearly & interesting way through animation, I'm B.pharma graduate, still i have doubt about staining, i understand in 4minutes. Thank u Dr. Bhanu prakash sir.

  • @indiraperezvalduciel7448
    @indiraperezvalduciel74483 жыл бұрын

    The animation is great, thanks for sharing it, it will help a lot to the courses and classes that I teach at the university (UCV). I will always take it into account and generate the reference quotes for your recognition. really thank you for such valuable information.

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Glad it was helpful!

  • @Bhartiya_housewife
    @Bhartiya_housewife4 ай бұрын

    People watching in 2024 ? For Neet ?

  • @yogesh_mali_77

    @yogesh_mali_77

    27 күн бұрын

    For biotechnology

  • @sonnybrown8913

    @sonnybrown8913

    24 күн бұрын

    For lab😅

  • @Ar-em1wl
    @Ar-em1wl3 ай бұрын

    Day before exam❤

  • @freshprince3015

    @freshprince3015

    2 ай бұрын

    About to start bsc microbiology. How was the exams?

  • @tejaspalagummi3237
    @tejaspalagummi32374 ай бұрын

    So much faster than reading my Micro textbook... thanks a LOT, Sir 😁👍

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 ай бұрын

    Glad to help!

  • @alicetabbara-safid4559
    @alicetabbara-safid45594 жыл бұрын

    Excellent presentation, this will allow my students a clear understanding of how the mordant works in Gram staining. Thank you.

  • @joel_maliyekal7464
    @joel_maliyekal74643 жыл бұрын

    Very use full

  • @evavassalou2126
    @evavassalou21263 жыл бұрын

    Thank you for the presentation, it will be very heplful to my students ( with reference quote for you)

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Ur most welcome

  • @kalpitthalor4474
    @kalpitthalor44748 ай бұрын

    thank you sir for this video I understand this concept in just 4-5 minutes that i am trying to understand from half an hour.

  • @hello-ll5ub
    @hello-ll5ub9 ай бұрын

    دكتوره خديجه رفعت مشاهداتكم

  • @ananyasharma6193
    @ananyasharma619311 ай бұрын

    Thankyou so much for this video! Now I feel ready to score full in my Biotechnology Practical tomorrow! The greatest thing is not just the animation but also those little concepts in every step ! Thankyou so much once again!!

  • @doctorbhanuprakash

    @doctorbhanuprakash

    11 ай бұрын

    Glad it was helpful!

  • @ananyasharma6193

    @ananyasharma6193

    11 ай бұрын

    It indeed was !

  • @martinaotoo6167

    @martinaotoo6167

    8 ай бұрын

    ​@@doctorbhanuprakashit was really helpful thanks a lot

  • @aaronninolanante7069
    @aaronninolanante70697 ай бұрын

    Good luck on my practical exam

  • @Abinesh13
    @Abinesh13 Жыл бұрын

    Thank you it is help to us

  • @ragini2008
    @ragini2008 Жыл бұрын

    Thanks❤

  • @medzoklife
    @medzoklife4 жыл бұрын

    Crystal clear

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    Thank u

  • @kashiftanha6631
    @kashiftanha663126 күн бұрын

    Thanks

  • @THANESHVARIAPMOGANARAJAMoe
    @THANESHVARIAPMOGANARAJAMoeАй бұрын

    During the Gram staining procedure, typically, about 2-3 drops of alcohol (ethanol or acetone) are used for the decolorization step. This is done to ensure the decolorizer spreads evenly across the slide to differentiate between Gram-positive and Gram-negative bacteria effectively. It's important to apply the drops slowly and observe the slide, as over-decolorizing can lead to false results.

  • @anuradhaanu4792
    @anuradhaanu47923 жыл бұрын

    Am happy to see this because am a microbiology student passed out student

  • @Shivsierra6
    @Shivsierra68 ай бұрын

    Having lab test today thank you ,stressed out right now

  • @doctorbhanuprakash

    @doctorbhanuprakash

    8 ай бұрын

    You got this!

  • @maryamnashwanlaso7567
    @maryamnashwanlaso75674 жыл бұрын

    Clearly understood thank you

  • @Lucifer-yi2bh

    @Lucifer-yi2bh

    3 жыл бұрын

    Same 2 u maryam, do you read in 11th class?

  • @bhavikapatel6835
    @bhavikapatel68353 жыл бұрын

    Thank you for your presentation it is very helpful for my students to understand gram stain

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    It's my pleasure

  • @songsofallages1528
    @songsofallages1528 Жыл бұрын

    I am Bangladeshi...I am a student of microbilogy..This is very helpful video for me

  • @BioBloom10M
    @BioBloom10M8 ай бұрын

    Thank You Mam Very fruitful❤

  • @mazbaljam891
    @mazbaljam8913 жыл бұрын

    Thanks 👍

  • @KhanKhan-gj3jp
    @KhanKhan-gj3jp2 жыл бұрын

    Bundle of thanx for excellent presentation

  • @kenechipreciousigwe9267
    @kenechipreciousigwe92674 ай бұрын

    Very precise video❤

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 ай бұрын

    Glad you liked it

  • @dipalirane96
    @dipalirane963 жыл бұрын

    Excellent explanation Thank you so much!!

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Ur most welcome

  • @kohilaramadas6525
    @kohilaramadas65253 жыл бұрын

    Microbiologist👍

  • @BlackZero880
    @BlackZero8803 жыл бұрын

    Like your way of explanation...and voice

  • @vijender7179
    @vijender7179 Жыл бұрын

    Thank you 👍

  • @degefehabte6775
    @degefehabte67753 жыл бұрын

    thanks for your short and précised procedure

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    You are welcome!

  • @yashtaylor4875
    @yashtaylor48753 жыл бұрын

    Best video ever

  • @comedyfunny145
    @comedyfunny1452 ай бұрын

    thank you this is very good video

  • @doctorbhanuprakash

    @doctorbhanuprakash

    2 ай бұрын

    Glad you liked it!

  • @daivikakagathara8409
    @daivikakagathara84094 жыл бұрын

    Nice vedio Everything is clear Thank you so much

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    Most welcome 😊

  • @ePlantHealth
    @ePlantHealth3 жыл бұрын

    Great video with great detail. Thank you.

  • @uroojaijazkhowaja
    @uroojaijazkhowaja3 жыл бұрын

    It is so helpful!

  • @Shortscience_I
    @Shortscience_I5 ай бұрын

    best

  • @Jaguar-lq3tp
    @Jaguar-lq3tp2 ай бұрын

    Great video👏👏,I'm sending this to my students as they had doubts on this topic

  • @doctorbhanuprakash

    @doctorbhanuprakash

    2 ай бұрын

    Glad it was helpful!

  • @supreethayadav5585
    @supreethayadav55853 жыл бұрын

    Super lecture..tq so much Pls do upload all bacteria

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Suree

  • @malignanttomar
    @malignanttomar4 жыл бұрын

    Very nice 👌😊👌😊👌😊👍

  • @hassanprince5785
    @hassanprince578511 ай бұрын

    Thanks 😊

  • @doctorbhanuprakash

    @doctorbhanuprakash

    11 ай бұрын

    Welcome 😊

  • @bilileb.5446
    @bilileb.54464 жыл бұрын

    Thanks 🙏 that helps a lot.

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    🤝🤝🤝

  • @humphreygaliwango566
    @humphreygaliwango5663 жыл бұрын

    On point. Asante

  • @dhakshathar8192
    @dhakshathar81923 жыл бұрын

    Thank you

  • @asnarafeek1370
    @asnarafeek13703 жыл бұрын

    Thank u for this video....👍🔥

  • @samasdinesh
    @samasdinesh3 жыл бұрын

    very nice slide thanks

  • @saurabhsingh5408
    @saurabhsingh54083 жыл бұрын

    Animation are to good, Thank you👍

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Glad you like them!

  • @DipaSaha-wd8qo
    @DipaSaha-wd8qo8 ай бұрын

    Nicely presented,, thanks for sharing this video😊

  • @doctorbhanuprakash

    @doctorbhanuprakash

    8 ай бұрын

    My pleasure 😊

  • @prajwalsinge9899
    @prajwalsinge9899 Жыл бұрын

    Thanks you mam

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Most welcome 😊

  • @jirdefanax4269
    @jirdefanax42693 жыл бұрын

    I understood thank you

  • @polipallivijaya1432
    @polipallivijaya143210 ай бұрын

    Nice Video Explaned in Easy way Thank You

  • @doctorbhanuprakash

    @doctorbhanuprakash

    10 ай бұрын

    You are most welcome

  • @mssmicrobiology12
    @mssmicrobiology123 жыл бұрын

    Best animations

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Tqu

  • @sethdjanboakye
    @sethdjanboakye Жыл бұрын

    Great Explanation. Thanks...

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Ur most welcome

  • @AhmadIlyas-fe6ll
    @AhmadIlyas-fe6llАй бұрын

    Good discusing

  • @Shakeel-
    @Shakeel-3 жыл бұрын

    thank u so much..

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Welcome 😊

  • @daljeetkaur6838
    @daljeetkaur68383 жыл бұрын

    Thanku sir ❤️

  • @komaljadhav3980
    @komaljadhav398010 ай бұрын

    This explanation was just amazing. Thank you for the video

  • @samahahmed1673
    @samahahmed16733 жыл бұрын

    Thank you so much ❤️❤️

  • @ranwarpasha3282
    @ranwarpasha32824 жыл бұрын

    Any video abt acid fast staining

  • @salmahmouz8092
    @salmahmouz8092 Жыл бұрын

    What happens if you use acid alcohol as the decolorizing agent in spore staining?

  • @reem8288
    @reem82883 жыл бұрын

    Thank you. It’s really helpful

  • @MohamedAli-kc1yy
    @MohamedAli-kc1yy Жыл бұрын

    thats fascinating

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Tysm

  • @jia3871
    @jia38713 жыл бұрын

    it helped a lot. thank you.

  • @biofriendz7958
    @biofriendz79582 жыл бұрын

    Thanks.👍 from sri lanka

  • @isawimedic
    @isawimedic2 жыл бұрын

    You are amazing God save you 🇮🇶

  • @AL_ayyubi1137
    @AL_ayyubi1137 Жыл бұрын

    A short video and useful to be learned on it Good job 👍

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Glad to hear that

  • @bernicelartey4693
    @bernicelartey4693 Жыл бұрын

    Thanks a lot. This has made made understand it very well

  • @Pharmakonacademy1
    @Pharmakonacademy14 жыл бұрын

    Ty sir

  • @akshayadiaries5144
    @akshayadiaries51444 жыл бұрын

    It's really very helpful

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    Tysm

  • @incarnatedguy7818
    @incarnatedguy781810 ай бұрын

    Very well Explanation ❤

  • @doctorbhanuprakash

    @doctorbhanuprakash

    10 ай бұрын

    Glad you liked it

  • @annthole8107
    @annthole81073 жыл бұрын

    Great video! Thank you

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Most welcome

  • @MariaAsim-wy5zy
    @MariaAsim-wy5zy4 жыл бұрын

    Thanks this video help us a lot to understand 🤩

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    Glad it was helpful!

  • @elbertek5836
    @elbertek58363 жыл бұрын

    Teşekkürler hanımefendi çok iyi anlatmışsınız

  • @Hayancreations
    @Hayancreations4 жыл бұрын

    nice

  • @thotapragnya278
    @thotapragnya2783 жыл бұрын

    Thank you so much for this video

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Most welcome 😊

  • @muhammadpervez6357
    @muhammadpervez6357 Жыл бұрын

    Good explanation 😊❤

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Thank you 🙂

  • @muhammadpervez6357

    @muhammadpervez6357

    Жыл бұрын

    @@doctorbhanuprakash u deserve this explanation is just amazing

  • @mfaizan8228
    @mfaizan82284 жыл бұрын

    Amazing

  • @uboyraz
    @uboyraz3 жыл бұрын

    Amazing video. Thank you

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Ur welcome

  • @yellankirevathi8099
    @yellankirevathi80993 жыл бұрын

    Excellent video

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Tysm

  • @nida124
    @nida1243 жыл бұрын

    In gram staining technique what is a physical mordent? And what is a chemical mordent? Can any body answer??????😊

  • @yasminhosam5951
    @yasminhosam59513 жыл бұрын

    This is super helpful thank you very much it really helped

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Glad to hear it!

  • @bernicelartey4693
    @bernicelartey4693 Жыл бұрын

    Very precise and understandable. Thanks

  • @luisaodi779
    @luisaodi7793 жыл бұрын

    Thank you 😊

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    You're welcome 😊

  • @carlosmarioocanagonzalez2314
    @carlosmarioocanagonzalez23142 жыл бұрын

    En mi experiencia realmente no es necesario cronometrar exactamente el tiempo con cristal violeta, iodo lugol y safranina debe de dejarse mínimo un minuto, pero realmente máximo tiempo no hay, y la decoloración no debe de ser hasta que deje de escurrir cristal violeta porque muchas veces y aunque se usen cepas jóvenes, se corre el riesgo de que se decolore a la fuerza, lo mejor es dejar derramar el chorrito de decolorante desde el inicio de la laminilla y permitir que llegue hasta el final de la misma, pasando por eso frotis ( que debe ser de buen tamaño y grosor) y de inmediato enjuagar. Crees ese es el "secreto" para una buena tinción.

  • @KhanKhan-gj3jp
    @KhanKhan-gj3jp2 жыл бұрын

    Superb

  • @joyce6801
    @joyce68013 жыл бұрын

    Thank you sir

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Welcome

  • @shehrozkhan107
    @shehrozkhan1073 жыл бұрын

    Thanks it helped me

  • @user-ec1uy8jl3i
    @user-ec1uy8jl3i Жыл бұрын

    Amaaaaaaaaazing ❤❤❤❤❤❤❤Thank you so much for this helpful video ❤

  • @doctorbhanuprakash

    @doctorbhanuprakash

    Жыл бұрын

    Glad it was helpful!

  • @meghna6223
    @meghna62233 жыл бұрын

    Thanks ☺️

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    ur most welcome

  • @meghna6223

    @meghna6223

    3 жыл бұрын

    🤗

  • @sree7834
    @sree78343 жыл бұрын

    Woow👍👍

  • @ZhiZhemini
    @ZhiZhemini3 жыл бұрын

    Very helpful

  • @englishwithravi4590
    @englishwithravi45904 жыл бұрын

    Thank you❤️👍

  • @doctorbhanuprakash

    @doctorbhanuprakash

    4 жыл бұрын

    You’re welcome 😊

  • @rajivdas8185
    @rajivdas81853 жыл бұрын

    Good

  • @doctorbhanuprakash

    @doctorbhanuprakash

    3 жыл бұрын

    Thanks

  • @ruchika_899
    @ruchika_899 Жыл бұрын

    Please tell me to prepare smear and microscopic examination of staphylococcus lactobacillus and e coli and gram staining teqniq both are same please tell me tomorrow is my practical 😭😭

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