Gram Staining Procedure Animation Microbiology - Principle, Procedure, Interpretation
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Gram staining procedure animation - Principle, Procedure, Interpretation
Principle:
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Staining is an auxiliary technique used in microscopic techniques used to enhance the clarity of the microscopic image. Stains and dyes are widely used in the scientific field to highlight the structure of biological specimens, cells, tissues etc.
The most widely used staining procedure in microbiology is the Gram stain, discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Gram-negative bacteria are decolorized by the alcohol, losing the color of the primary stain, purple. Gram-positive bacteria are not decolorized by alcohol and will remain as purple. After decolorization step, a counterstain is used to impart a pink color to the decolorized gram-negative organisms.
Importance of a Gram Stain:
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The Gram stain is a very important preliminary step in the initial characterization and classification of bacteria. It is also a key procedure in the identification of bacteria based on staining characteristics, enabling the bacteria to be examined using a light microscope. The bacteria present in an unstained smear are invisible when viewed using a light microscope. Once stained, the morphology and arrangement of the bacteria may be observed as well. Furthermore, it is also an important step in the screening of infectious agents in clinical specimens such as direct smears from a patient.
The Gram stain procedure enables bacteria to retain color of the stains, based on the differences in the chemical and physical properties of the cell wall.
1. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall. Example: Staphylococcus aureus
2. Gram negative bacteria: Stain red or pink due to retaining the counter staining dye called Safranin.
Example: Escherichia coli
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📌 𝐅𝐨𝐥𝐥𝐨𝐰 𝐨𝐧 𝐈𝐧𝐬𝐭𝐚𝐠𝐫𝐚𝐦:- instagram.com/drgbhanuprakash 📌𝗝𝗼𝗶𝗻 𝗢𝘂𝗿 𝗧𝗲𝗹𝗲𝗴𝗿𝗮𝗺 𝗖𝗵𝗮𝗻𝗻𝗲𝗹 𝗛𝗲𝗿𝗲:- t.me/bhanuprakashdr 📌𝗦𝘂𝗯𝘀𝗰𝗿𝗶𝗯𝗲 𝗧𝗼 𝗠𝘆 𝗠𝗮𝗶𝗹𝗶𝗻𝗴 𝗟𝗶𝘀𝘁:- linktr.ee/DrGBhanuprakash
This just explained it to me in 4 mins what my teacher couldn’t do in an hour! Thanks a lot 😇
@doctorbhanuprakash
3 жыл бұрын
You're very welcome!
@rose_-nb7nk
Жыл бұрын
😂😂😂😂😂
@Juliee1__
Жыл бұрын
True'😶😂
@educationlobby985
Жыл бұрын
Mine teachers couldn't understand it in a year 😂
@ajcreations2486
Жыл бұрын
True 🤣
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@lokeshaalone6550
4 жыл бұрын
Some video not show why
@ePlantHealth
3 жыл бұрын
Please, could you suggest me the software you are using to creat such animation videos.
@aftabahmedsoomro9141
3 жыл бұрын
.
I have seen many videos but this one is the best
@doctorbhanuprakash
4 жыл бұрын
thank you
Legend watching in 2024😂😂😂😂😂
Sir Your explaining topic vry clearly & interesting way through animation, I'm B.pharma graduate, still i have doubt about staining, i understand in 4minutes. Thank u Dr. Bhanu prakash sir.
The animation is great, thanks for sharing it, it will help a lot to the courses and classes that I teach at the university (UCV). I will always take it into account and generate the reference quotes for your recognition. really thank you for such valuable information.
@doctorbhanuprakash
3 жыл бұрын
Glad it was helpful!
People watching in 2024 ? For Neet ?
@yogesh_mali_77
27 күн бұрын
For biotechnology
@sonnybrown8913
24 күн бұрын
For lab😅
Day before exam❤
@freshprince3015
2 ай бұрын
About to start bsc microbiology. How was the exams?
So much faster than reading my Micro textbook... thanks a LOT, Sir 😁👍
@doctorbhanuprakash
4 ай бұрын
Glad to help!
Excellent presentation, this will allow my students a clear understanding of how the mordant works in Gram staining. Thank you.
Very use full
Thank you for the presentation, it will be very heplful to my students ( with reference quote for you)
@doctorbhanuprakash
3 жыл бұрын
Ur most welcome
thank you sir for this video I understand this concept in just 4-5 minutes that i am trying to understand from half an hour.
دكتوره خديجه رفعت مشاهداتكم
Thankyou so much for this video! Now I feel ready to score full in my Biotechnology Practical tomorrow! The greatest thing is not just the animation but also those little concepts in every step ! Thankyou so much once again!!
@doctorbhanuprakash
11 ай бұрын
Glad it was helpful!
@ananyasharma6193
11 ай бұрын
It indeed was !
@martinaotoo6167
8 ай бұрын
@@doctorbhanuprakashit was really helpful thanks a lot
Good luck on my practical exam
Thank you it is help to us
Thanks❤
Crystal clear
@doctorbhanuprakash
4 жыл бұрын
Thank u
Thanks
During the Gram staining procedure, typically, about 2-3 drops of alcohol (ethanol or acetone) are used for the decolorization step. This is done to ensure the decolorizer spreads evenly across the slide to differentiate between Gram-positive and Gram-negative bacteria effectively. It's important to apply the drops slowly and observe the slide, as over-decolorizing can lead to false results.
Am happy to see this because am a microbiology student passed out student
Having lab test today thank you ,stressed out right now
@doctorbhanuprakash
8 ай бұрын
You got this!
Clearly understood thank you
@Lucifer-yi2bh
3 жыл бұрын
Same 2 u maryam, do you read in 11th class?
Thank you for your presentation it is very helpful for my students to understand gram stain
@doctorbhanuprakash
3 жыл бұрын
It's my pleasure
I am Bangladeshi...I am a student of microbilogy..This is very helpful video for me
Thank You Mam Very fruitful❤
Thanks 👍
Bundle of thanx for excellent presentation
Very precise video❤
@doctorbhanuprakash
4 ай бұрын
Glad you liked it
Excellent explanation Thank you so much!!
@doctorbhanuprakash
3 жыл бұрын
Ur most welcome
Microbiologist👍
Like your way of explanation...and voice
Thank you 👍
thanks for your short and précised procedure
@doctorbhanuprakash
3 жыл бұрын
You are welcome!
Best video ever
thank you this is very good video
@doctorbhanuprakash
2 ай бұрын
Glad you liked it!
Nice vedio Everything is clear Thank you so much
@doctorbhanuprakash
4 жыл бұрын
Most welcome 😊
Great video with great detail. Thank you.
It is so helpful!
best
Great video👏👏,I'm sending this to my students as they had doubts on this topic
@doctorbhanuprakash
2 ай бұрын
Glad it was helpful!
Super lecture..tq so much Pls do upload all bacteria
@doctorbhanuprakash
3 жыл бұрын
Suree
Very nice 👌😊👌😊👌😊👍
Thanks 😊
@doctorbhanuprakash
11 ай бұрын
Welcome 😊
Thanks 🙏 that helps a lot.
@doctorbhanuprakash
4 жыл бұрын
🤝🤝🤝
On point. Asante
Thank you
Thank u for this video....👍🔥
very nice slide thanks
Animation are to good, Thank you👍
@doctorbhanuprakash
3 жыл бұрын
Glad you like them!
Nicely presented,, thanks for sharing this video😊
@doctorbhanuprakash
8 ай бұрын
My pleasure 😊
Thanks you mam
@doctorbhanuprakash
Жыл бұрын
Most welcome 😊
I understood thank you
Nice Video Explaned in Easy way Thank You
@doctorbhanuprakash
10 ай бұрын
You are most welcome
Best animations
@doctorbhanuprakash
3 жыл бұрын
Tqu
Great Explanation. Thanks...
@doctorbhanuprakash
Жыл бұрын
Ur most welcome
Good discusing
thank u so much..
@doctorbhanuprakash
3 жыл бұрын
Welcome 😊
Thanku sir ❤️
This explanation was just amazing. Thank you for the video
Thank you so much ❤️❤️
Any video abt acid fast staining
What happens if you use acid alcohol as the decolorizing agent in spore staining?
Thank you. It’s really helpful
thats fascinating
@doctorbhanuprakash
Жыл бұрын
Tysm
it helped a lot. thank you.
Thanks.👍 from sri lanka
You are amazing God save you 🇮🇶
A short video and useful to be learned on it Good job 👍
@doctorbhanuprakash
Жыл бұрын
Glad to hear that
Thanks a lot. This has made made understand it very well
Ty sir
It's really very helpful
@doctorbhanuprakash
4 жыл бұрын
Tysm
Very well Explanation ❤
@doctorbhanuprakash
10 ай бұрын
Glad you liked it
Great video! Thank you
@doctorbhanuprakash
3 жыл бұрын
Most welcome
Thanks this video help us a lot to understand 🤩
@doctorbhanuprakash
4 жыл бұрын
Glad it was helpful!
Teşekkürler hanımefendi çok iyi anlatmışsınız
nice
Thank you so much for this video
@doctorbhanuprakash
3 жыл бұрын
Most welcome 😊
Good explanation 😊❤
@doctorbhanuprakash
Жыл бұрын
Thank you 🙂
@muhammadpervez6357
Жыл бұрын
@@doctorbhanuprakash u deserve this explanation is just amazing
Amazing
Amazing video. Thank you
@doctorbhanuprakash
3 жыл бұрын
Ur welcome
Excellent video
@doctorbhanuprakash
3 жыл бұрын
Tysm
In gram staining technique what is a physical mordent? And what is a chemical mordent? Can any body answer??????😊
This is super helpful thank you very much it really helped
@doctorbhanuprakash
3 жыл бұрын
Glad to hear it!
Very precise and understandable. Thanks
Thank you 😊
@doctorbhanuprakash
3 жыл бұрын
You're welcome 😊
En mi experiencia realmente no es necesario cronometrar exactamente el tiempo con cristal violeta, iodo lugol y safranina debe de dejarse mínimo un minuto, pero realmente máximo tiempo no hay, y la decoloración no debe de ser hasta que deje de escurrir cristal violeta porque muchas veces y aunque se usen cepas jóvenes, se corre el riesgo de que se decolore a la fuerza, lo mejor es dejar derramar el chorrito de decolorante desde el inicio de la laminilla y permitir que llegue hasta el final de la misma, pasando por eso frotis ( que debe ser de buen tamaño y grosor) y de inmediato enjuagar. Crees ese es el "secreto" para una buena tinción.
Superb
Thank you sir
@doctorbhanuprakash
3 жыл бұрын
Welcome
Thanks it helped me
Amaaaaaaaaazing ❤❤❤❤❤❤❤Thank you so much for this helpful video ❤
@doctorbhanuprakash
Жыл бұрын
Glad it was helpful!
Thanks ☺️
@doctorbhanuprakash
3 жыл бұрын
ur most welcome
@meghna6223
3 жыл бұрын
🤗
Woow👍👍
Very helpful
Thank you❤️👍
@doctorbhanuprakash
4 жыл бұрын
You’re welcome 😊
Good
@doctorbhanuprakash
3 жыл бұрын
Thanks
Please tell me to prepare smear and microscopic examination of staphylococcus lactobacillus and e coli and gram staining teqniq both are same please tell me tomorrow is my practical 😭😭