Gram staining for differentiating bacterial species
Gram staining (or Gram's method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor, Hans Christian Gram.
Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting peptidoglycan, which is present in a thick layer in Gram positive bacteria.[1] A Gram positive results in a purple/blue color while a Gram negative results in a pink/red color.
The Gram stain is almost always the first step in the identification of a bacterial organism. While Gram staining is a valuable diagnostic tool in both clinical and research settings, not all bacteria can be definitively classified by this technique. This gives rise to Gram-variable and Gram-indeterminate groups as well.
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That's nice 😊...we can learn this by a simple sentence Come In And Stain.. C- Crystal violet I - iodine A - alcohol (ethanol) S- safranin
@sanfordngandu6375
4 жыл бұрын
you are the best bro
@nessaeks3853
4 жыл бұрын
Medaase bebree😊
@nocmemer4686
4 жыл бұрын
Yaha bhi aap😂
@poojapareek1386
4 жыл бұрын
@@nocmemer4686 😂😂 koi Bach na Jaye sikhne se
@nocmemer4686
4 жыл бұрын
😂😂😂
Its been nine years of this video and I found today it very helpful. Keep it up bro.
The platinum loop after red hot should be kept nearer to the flame for few seconds so that it doesn't kills the bacteria which should be taken next for observation/differentiation.Nice informative video.
@GenomicsLab
5 жыл бұрын
thanks we will correct it
Thank you so much for clarifying the concept of gram staining it's really very helpful 🙏
This video gives information about differentiating the gram positive and gram negative bacteria... thanks for the information...
Thanks a lot bro, you've just uncomplicated things for me. Today I won't be lost during practical time !!!
Thanks bro. Now I won't look stupid in my lab, I alway do :(
@selvamselva1178
5 жыл бұрын
Good
@FranciscoFlores-kl4lw
5 жыл бұрын
huele a obo
@thaboedgar5113
5 жыл бұрын
Your not alone
@h.e.r9368
5 жыл бұрын
Me too ugh
@freebird1555
Жыл бұрын
Hii
That's gonna help a lot with my exam tomorrow :D
@cherieannearevalo9670
7 жыл бұрын
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@justanoobartist4665
6 жыл бұрын
ExWeeaboo on the 2nd thought no!!
@JakirHussain-bc3ze
5 жыл бұрын
same pinch i too have xam tommorrow
@sreeluanayadi9994
5 жыл бұрын
Thanku sir this video help for my lab examm
@basirJabar
5 жыл бұрын
ExWeeaboo subscribe me please
Super sir am studying BSC nursing this is very useful in my exams
@GenomicsLab
5 жыл бұрын
thanks for your kind words and support for this video.pl share to the needy persons
@basirJabar
5 жыл бұрын
Nagalaxmi Jelli subscribe me please
The techniques are good. But I think this staff should mark the position of bacteria by drawing a circle on the slide before starting stain them.
@user-mf9wy6rs6t
4 жыл бұрын
not necessarily
You do not need to let it air dry. You do the heat fix to not only dry it but it kills the bacteria and makes it stick to the slide.
@parisindha7779
6 жыл бұрын
Really??
@apurvashyam620
5 жыл бұрын
Yes she is right
@lawalabdullahibela8997
5 жыл бұрын
Haley Green you're right
@aliahmadmughal6455
5 жыл бұрын
Right
@basirJabar
5 жыл бұрын
Haley Green Argonaut subscribe me please
Gram positive p= purple Gram negative,✖️= red.
@drkishalinishalini
5 жыл бұрын
thank u
@craerae
5 жыл бұрын
Pink
@altafhussain-qr4sn
5 жыл бұрын
Gram positive
@rajushankars8603
4 жыл бұрын
Gram positive bacteria are blue or purple colour Ans gram negative bacteria are red or pink
This is the very nice video presentation of gram staining for differentiating bacterial species, in this video it's very easy to understand the procedure because all things are in sequential manner
Thank you sir for giving better differences between gram positive and negative bacteria
Superb. .....v. v. Informative. And. Beautifully. Explained.......keep. it. Up☺
Thanks for sharing video . I understood gram staining
Thank you very much this was helpful I'm going to do this practical today in my microbiology like now at 8
@jenninatorx3
4 жыл бұрын
Sibusiso Sibiya random but did you use the same times as the guy in the video did and succeed? (30 seconds methlyne blue, 1 min iodine, rinse till clear with ethanol, then 1 minute saffranin?) My teacher told us (1 minutes MB, 2-5 minutes of iodine, 6 seconds of ethanol and then 2 minutes of saffranin) and I spent the whole lab yet can only achieve all purple :( so Im now wondering if i put the stains on too long and then the ethanol not enough. 🤷🏻♀️
@nathanot1076
4 жыл бұрын
jennifer pan it usually means you didn’t run the enough ethanol so next time run it till you don’t see any blue color at the bottom and immediately rinse
Than q sir im be happy because gram staining is completed
Thanks bro . You explained simply as understand . Good work
wao sir ,awsm vdo...
I've already done biotechnology lab but I want to listen again . that helps me to remember in exam
This is so helpful.Only God can reward you
Thxxx lecture daan kya ltyaa broooi❤❤❤
mujhe apka vedio bahut acha laga please esi tarah culture and isolation of bacteria or cocci bacilli ke bhi vedio dal di jiye or ho sake to hindi me dalo to bahut acha hoga
@justinpaul6998
7 жыл бұрын
don't u know english?
nice and informative video. thanks for sharing.
nice presentation as it gives the actual pictorial knowledge of gram staining, thnks for video..
This was very well presented and easy to understand. Well done! Thank you.
Yea, I have a practical in 2 hour's and thank you ♥️😂
its gud to got more information about staining of bacteria within short time wel thnx for such creative video n sharng with us
what a super.appreciating you bro.. keep it up for more videos
such an useful viedo thanks 4 uploading it...
@krishnadeokumar370
6 жыл бұрын
Christina Susairaj
Last pic of bacteria are real ??
Please do practicals of class 11 CBSE....... I understood a lot from this video....hats off to you!!!
@altafhussain-qr4sn
5 жыл бұрын
goood wark
Very good
@GenomicsLab
5 жыл бұрын
keep encouraging
@swarajganiga3127
5 жыл бұрын
@@GenomicsLab make videos on microbiology instrumentations
useful and helpful vedio
khatanak exlpaination sir good
Supper explanation easily I understand
Very very useful..Please upload many vid like this
Useful video
SO fantastic video
Very nice vidio and nice explanation
Khup Chan dada
Excellent 👌thanks for this video
Very nice 👌👌
Good and great video..thx sir
very nice
Thanks a lot...! 😭😭😭 Now I really understand... 😂 Now this is my 11th hour preparation ... I'm confident!
Very good explanation and proper video
Thanks for this video... Actually I wasn't getting thst what is gram positive and negative and for whst purpose it is used for. But now I get to now after watching this video:)
very nice video
Nice explained sir
very nice Sir
Thank you soo much sir is video ne meri confusion door kr di thanx again 😊
@GenomicsLab
5 жыл бұрын
thanks for watching
@altafhussain-qr4sn
5 жыл бұрын
yu
Very easy procedure for gram staining understanding
Vary Nice sir
Exellant jazak allah khiraa
Very informative and interesting video.thanks a lot.
THNKXXXXX.THIS VIDEO MADE ME UNDERSTAND WITH GOD'S HELP.....
Thank you soo much..... This is a very helpful vedio....!!
nycely done ....tq so much 4 this beautiful work
very good work...... i m really amazed..
Nice explanation
Thankyou for the clear demonstration. Appreciate your video.
Very good bro
Mashalla Nice
I personally love videos they lecture me ,thanks
जी नमस्कार क्या हम किसी वैक्टीरिया को मल्टीपलाई करने के लिए दो किलो गुड़ लेते अगर हम उन्हीं वैक्टीरिया को मल्टीपलाई होने के लिए आधा किलो गुड़ लेते हैं तो क्या वैक्टीरिया की संख्या कम नहीं होगी। plz reply
Thanks sir ,i am a food technology student ,it will help me
Gram negative pink and red color species individual different say plz
Thank you soo much:) That's gonna help me tomorrow:)
Thank you so much sir very nice video no any doubts
Very useful for me...thank u soo much sir for sharing this vdo..
@oluwafunmitoolaleye3338
Жыл бұрын
Thanks .
Its very useful.Thank you🥰
It will help to student to know about the gram staining for what purpose it is used
Very nice explanation sir thank you sir
Iam really appreciate your effort for improving our knowledge ,thanks u all
After looping the culture in slide, after dryness of the slide, have to show in fire lamp on opposite side are culture side ???
waow great
nice video
I am like your English language
Wonderful.....!
Awsm explanation 👌👌
Thanks a lot
Anyways for heat fixing you shouldnt heat fix too long as you might crack the glass slide instead. Instead just move in and out the flame 4-5 times can already.
Sir make on more sample on pus blood ,other swab like conjunctival swab etc culture sensitivity and types of bacteria which show on the microscopy thanku keep it up
Aren't we have to at least wait for 10 second for the loop to cool down before extracting the bacterial culture?
@MaveHo
5 жыл бұрын
Not really 10 seconds, as long as you test it on the agar, it doesnt burn the agar it should be cool enough to extract a bacterial culture
@basirJabar
5 жыл бұрын
Mervyn Anip Argonaut subscribe me please
@venkatatejaswi6539
4 жыл бұрын
Yes we have to wait orelse the inoculation loop which was incinerated,it will be hot so when you keep that loop in the bacterial culture there may be chance in which bacteria may die.
@Tom_says_hi_
Жыл бұрын
English 😮
Chal bahi hogai kal ki exam ki tyari now i can sleep mazy sy :D
Great explanation sir
Good job
very nice and useful video. Thanks
Thank you so much Bro ....Tiz gonna help me me tomorrow 😊
Thank very much for this its much helpful to all lab tech student
Good 👍
Clear explanation
very nyc so good
Nice👍👍👍👍
Thank you sir
Thanks a lot bro ,use the plate (colonies)for isolation in order to get pure colony for further biochemical test like indole test.but what sample(specimen) is that?
Thanks for making this video sir I could understand easily
Thank u sir Ur video is really helpful ... keep uploading such video
@altafhussain-qr4sn
5 жыл бұрын
good