Four Quadrant Streak procedure - How to properly streak a Petri plate for isolated colonies

Ғылым және технология

In this video you will learn how to use proper lab technique to streak a Petri plate for isolated colonies using the four quadrant streak method. To learn more about Hardy Diagnostics visit our new website. 👉 go.hardydiagnostics.com/yt-ha...
📲 General Laboratory Supplies: hardydiagnostics.com/lab-supp...
🖥 View the tutorial on our blog
hardydiagnostics.com/blog/pet...
We are 100% employee owned and have been serving microbiologists for over 40 years.
To learn more about Hardy Diagnostics and the products we offer, visit us at hardydiagnostics.com/
Hardy Diagnostics is your complete Microbiology supplier.
🖱 Check out our full line up of inoculating loops by clicking the link below.
hardydiagnostics.com/lab-supp...
You can also see our complete list of catalogs in the link below.
go.hardydiagnostics.com/yt-ha...
Contact us with any questions:
hardydiagnostics.com/contact-us
📍Follow us:
LinkedIn - / hardydiagnostics
Facebook - / hardydiagnostics
Instagram - / hardydiagnostics
X - X.com/HardyDiagnostic
00:00 Intro to streaking an agar plate
00:21 What to know before beginning
01:01 Preparation
01:50 Four quadrant streak diagram
02:29 Types of loops
03:28 Collecting a sample
03:43 How to do a four Quadrant Streak
04:36 Using a swab
05:28 Incubating the plate
05:43 Using a plastic loop
06:24 Close and ordering info
Do you prefer reading when learning? Here is our blog:
hardydiagnostics.com/blog
Filmed and Edited by Brian Tom
#Streakplate #Bacteria #Microbiology #HardyDiagnostics #4quadrant
Tags:
quadrant streak plate, streaking agar plate, agar plates, streaking agar plates, quadrant streaking, how to streak on agar, what is a four quadrant streak, four quad streak, how to streak a petri plate, petri plate streaking, streak plate method english, quadrant streak, spread plate technique, streek agar plate, what is a four quadrant streak, what is a four quad streak, help doing a four quadrant streak, study four quadrant streak, exam four quadrant streak

Пікірлер: 111

  • @Ms1101997
    @Ms1101997 Жыл бұрын

    I’m a new microbiology student and I’ll do this for my tomorrow’s lab 😅 I was confused but thanks god I found this video

  • @HardyDx

    @HardyDx

    Жыл бұрын

    We love helping new micro enthusiasts! Feel free to browse our other tutorials for your class too!

  • @winispecial

    @winispecial

    Жыл бұрын

    "Thank God you found this video "

  • @CrankyRayy

    @CrankyRayy

    Жыл бұрын

    @@winispecial " " Thank God you found this video " "

  • @mdraihan1582

    @mdraihan1582

    4 ай бұрын

    What's your country name?

  • @Ms1101997

    @Ms1101997

    4 ай бұрын

    @@mdraihan1582 I’m from the US, California

  • @notjeb_9409
    @notjeb_9409 Жыл бұрын

    its so wild how a lot of ppl commenting here r college students or professionals n here i am watching this for our lab activity in my high school biology class. cool video, helps a lot

  • @lorettarodman9117
    @lorettarodman91177 сағат бұрын

    I was taught a very different way, always remember that there is more than one way to streak plates everyone! Find what works best for you. Also if you have a bacteria that really sucks at growing on plates from freezer stock I’ve found that not flaming between streaks helps with that

  • @Dananq98
    @Dananq98 Жыл бұрын

    So useful description, thanks a lot 👏🏻

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Glad it was helpful!

  • @daliadivicpuskarski7453
    @daliadivicpuskarski7453 Жыл бұрын

    Yesteday I streaked a Petri dish for the first time in my life. I've watched this video before doing it and it really helped me 🧫 Thank you 😊

  • @HardyDx

    @HardyDx

    Жыл бұрын

    That's wonderful. Glad it helped!

  • @Faceless_Chat
    @Faceless_Chat2 ай бұрын

    Very helpful. I have a "unknown bacterium" assignment that starts today. This really helped bc I already forgot you have to sterilize the loop between streaking...

  • @Cyan-J-e5u
    @Cyan-J-e5u5 ай бұрын

    It's self explanatory and the visuals, helps for easy remembrance. Thanks.

  • @HardyDx

    @HardyDx

    5 ай бұрын

    You are very welcome. I am glad our video was useful for you.

  • @ry_n007
    @ry_n0072 ай бұрын

    thank you for saving my ee broski 🙏🙏

  • @sloane.2747
    @sloane.2747 Жыл бұрын

    This was so much fun when I did it, microbiology was my literal favorite class my first semester of college

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Glad to hear it! Keep on learning!

  • @vennelagovindugari5420
    @vennelagovindugari54202 жыл бұрын

    Perfect!!

  • @ruchika_899
    @ruchika_899 Жыл бұрын

    Gorgeous explanation 💥❤️

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Thank you, glad you liked it!

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Жыл бұрын

    I really appreciate you sir.

  • @ayesha-it5yw
    @ayesha-it5yw Жыл бұрын

    I learnt this method today in my microbiology lab.

  • @HardyDx

    @HardyDx

    Жыл бұрын

    That's great Ayesha! Keep up the proper four quadrant streak method, you will be a pro in no time!

  • @leannajulien4175
    @leannajulien4175 Жыл бұрын

    I didn't know I have place it in the heat everything I learned something new. Thanks

  • @HardyDx

    @HardyDx

    Жыл бұрын

    We're always here to help you learn! We have a great Technical Services team should you ever have questions!

  • @nina-ge6mn
    @nina-ge6mn Жыл бұрын

    I love microbiology and honestly its just so satisfying to watch people streak plate I'm doing a microbiology project that involves e. Coli K-12 plating and im so excited

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Nina, we love projects for the tiny humans! Please feel free to reach out to your local sales rep for product, we'd love to help out the next generation of micro-microbiologists!

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Жыл бұрын

    It's absolutely Amazing sir.

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Thanks, it's our pleasure.

  • @clairem290
    @clairem290 Жыл бұрын

    I'm a college instructor and I'm sending this video to my students before they do their first-ever cultures!

  • @HardyDx

    @HardyDx

    Жыл бұрын

    We love our instructors and our students! Feel free to reach out to us should you need materials for your students as well!

  • @Flowers12660

    @Flowers12660

    4 ай бұрын

    Well as an instructor why don't you show your students this yourself? Isn't this supposed to be your job to teach? It would be alot better. I really dislike and hate how college so called instructors and lecturers are relying so much on youtube videos instead of doing the work themselves.

  • @clairem290

    @clairem290

    4 ай бұрын

    I did streak some plates myself, which came in handy later. However, a nice KZread video they could watch the night before was also really helpful. To make up for your previous instructors who didn't explicitly show you enough things, I will now show you how to spell the phrases "so-called" and "a lot."

  • @AL_ayyubi1137
    @AL_ayyubi1137 Жыл бұрын

    I'm medical laboratory technician(MLT) and 11 lecture is included for simple technique in tomorrow thank you for this video I appreciate it 🤩

  • @HardyDx

    @HardyDx

    Жыл бұрын

    You are welcome, I am glad the video was helpful. Keep up the great work as a MLT. You are important.

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Жыл бұрын

    It's well explained.wow

  • @ruchika_899
    @ruchika_899 Жыл бұрын

    Excellent explanation ❤

  • @HardyDx

    @HardyDx

    Жыл бұрын

    I am glad our video was useful for to you.

  • @johnson1262
    @johnson1262 Жыл бұрын

    My universities professor , didn’t tech to put the petri disk , up side down when going into incubator. Thank you for sharing.

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Oh no! When media is incubated "upside down" like that, with the media facing upwards, condensation can accumulate on the face of the agar and inhibit proper growth and developing colonies. If you invert your plates, you'll definitely have better-looking plates than the rest of your class!

  • @tayyabirshad3029
    @tayyabirshad3029 Жыл бұрын

    Today may microbiology practical exam it's help alot!!🥰🥰🥰🥰

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Glad to hear the video was useful. You are very welcome. Good luck with your exam. You will do great!

  • @DanTheisen
    @DanTheisen2 жыл бұрын

    This is an excellent video. Thanks, Hardy for showing us how this is properly done. Much more to it that a squiggle of bugs on a petri plate!

  • @HardyDx

    @HardyDx

    2 жыл бұрын

    Glad it helped!

  • @DanTheisen

    @DanTheisen

    2 жыл бұрын

    As required by law, I’m disclosing that I was employed by Hardy Diagnostics at the time of this comment.

  • @Sean-ur4cb

    @Sean-ur4cb

    Жыл бұрын

    @@DanTheisen what did you sign that told you to disclose this info?

  • @DanTheisen

    @DanTheisen

    Жыл бұрын

    I don’t recall where I saw it but this is, as far as I know, meant to prevent large companies from having their employees promote their products as if they are customers. I have not signed anything from the company compelling either a comment or a disclaimer about the comment. The law as I read it made sense to me from a consumer protection point of view, which is why I happily added it.

  • @Sean-ur4cb

    @Sean-ur4cb

    Жыл бұрын

    @@DanTheisen ahh I see, thanks for the reply. Have a good day!

  • @jessical4866
    @jessical4866 Жыл бұрын

    I’m not a teacher or a student… why am I so fascinated with this? 😂

  • @HardyDx

    @HardyDx

    Жыл бұрын

    I mean we can't blame you, we do this for a living. Our whole lives are this, welcome to the obsession!

  • @GaneshKunayak

    @GaneshKunayak

    Жыл бұрын

    Who are you 🤣😂

  • @affiliatemarketinginnigeri7617

    @affiliatemarketinginnigeri7617

    Жыл бұрын

    I'm a passer-by as well and I am entranced ❤️

  • @jennisagala1263
    @jennisagala1263 Жыл бұрын

    thanks a lot, i hope, my thesis will be finisih

  • @nithinmuluka7987
    @nithinmuluka7987 Жыл бұрын

    Thank you sir very nice video... Pls upload more videos

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Yes, stay tuned. More video are on the way!

  • @Manthab
    @Manthab Жыл бұрын

    Don’t know how I got here at 1am but it was fascinating and informative if I ever need to know this I’m covered😁

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Welcome down the rabbit hole of microbiology, Mantha! We've got a whole channel of videos if you ever need more obscure information to get you even more hooked 😃!

  • @1alex92
    @1alex92 Жыл бұрын

    Wish I seen this 2 weeks ago when I had a test! ...nevertheless.. I just subscribed ☺️

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Welcome to the online Hardy family! Stay tuned for more great content soon to come!

  • @apokalypthoapokalypsys9573
    @apokalypthoapokalypsys9573 Жыл бұрын

    I'm in an IT MSc but this is perfect ASMR

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Love it! That's a whole new industry we should break into!

  • @Szaisthatyou
    @Szaisthatyou Жыл бұрын

    I love my major ❤

  • @FarzadSalim
    @FarzadSalim Жыл бұрын

    thank you

  • @HardyDx

    @HardyDx

    Жыл бұрын

    You're welcome.

  • @anycamenzind
    @anycamenzind7 ай бұрын

    Will there be a video available that talks about the microscope and Khoeler illumination? Unfortunately, part of the practical microbiology and laboratory sessions were taken in times of pandemic. Likewise, I would greatly appreciate if you could explain how the UFC colony counter works. Your explanations are great! Thank you 🙌🙌🙌

  • @RaiSaysS
    @RaiSaysS2 жыл бұрын

    Thank you

  • @HardyDx

    @HardyDx

    2 жыл бұрын

    You're welcome.

  • @ceciliaovansa2738
    @ceciliaovansa27385 ай бұрын

    Nice one

  • @HardyDx

    @HardyDx

    5 ай бұрын

    Thanks for watching

  • @todddillerson4053
    @todddillerson4053 Жыл бұрын

    I do this every day for the hospital I work at :)

  • @HardyDx

    @HardyDx

    Жыл бұрын

    That's great! Heroes wear lab coats not capes. Which hospital do you work at?

  • @Hellamoody

    @Hellamoody

    Жыл бұрын

    @@HardyDx boundaries Dwight!

  • @AliyuNanaaishat
    @AliyuNanaaishat3 ай бұрын

    Please can I see a video that will teach me more on how to carry out indoor air quality using SDA and PDA

  • @user-ot7km4px5n
    @user-ot7km4px5n7 ай бұрын

    How do you report colony growth on a 4 way streaking? along with its size, shape, transparency, consistency, and color.. Thanks

  • @nangsanbhalangblah333
    @nangsanbhalangblah333 Жыл бұрын

    Great vid, I've a question though. Why is the media red in color? Is there a different type of agar used?

  • @918mounika7

    @918mounika7

    Жыл бұрын

    It's probably blood agar medium, blood added to regular nutrient agar medium

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Nangsanbhalang Blah, Great question about the coloration of the agar! This was streaked on our Blood Agar, an all-purpose growth agar with hemolytic properties. Some organisms will hemolyze the blood in the agar, leaving clear zones around the colonies, something we won't see on traditional TSA. Hope that helps!

  • @M313_1
    @M313_15 ай бұрын

    *ثانكيو* 🦦

  • @SOPHIA-yf7ws
    @SOPHIA-yf7ws10 ай бұрын

    A++++

  • @pastmonster446
    @pastmonster446 Жыл бұрын

    Watching this after I took my lab practical exam, and I definitely fucked it up

  • @joannadevung4358
    @joannadevung43583 ай бұрын

    If i accidentally tore the surface of the agar using the loop during streaking, will the bacteria colonies still able to grow?

  • @HardyDx

    @HardyDx

    2 ай бұрын

    Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

  • @sunny4483
    @sunny4483 Жыл бұрын

    Can we not streak circular on plate instead of streaking zig zag? I'm curious

  • @MrDubbindan

    @MrDubbindan

    8 ай бұрын

    There is an instrument that does streak with a plastic "comb" after pipetting a volume on a plate and it does make circles ... The Previ Isola

  • @kasisfile
    @kasisfile Жыл бұрын

    😮

  • @user-bw1uz7oy1w
    @user-bw1uz7oy1w7 ай бұрын

    Quand utiliser la methode en stries?

  • @mohanzm12
    @mohanzm12 Жыл бұрын

    What do we call the thing she used to flame the loop ? 😅

  • @DadaClaire

    @DadaClaire

    Жыл бұрын

    I'm curious too. I'm working with unicellular fungi and bacteria since time, streaking them to isolate but it's the first time ever I'm seeing that instrument.

  • @vanessadang4674

    @vanessadang4674

    Жыл бұрын

    It is called a bacticinerator :)

  • @DadaClaire

    @DadaClaire

    Жыл бұрын

    @@vanessadang4674 Thank you!

  • @HardyDx

    @HardyDx

    Жыл бұрын

    @Mohan The tube the loop was inserted into is a special type of sterilizer. Placing non-sterile metal loops into this tube for a few seconds will sterilize these loops so you can re-use these again and again. Hope that answers your questions!

  • @mohanzm12

    @mohanzm12

    Жыл бұрын

    @@vanessadang4674 wow .thank you

  • @GaneshKunayak
    @GaneshKunayak Жыл бұрын

    Bdw i think this must be carried out inside a laminar air flow

  • @chrisk6469
    @chrisk6469 Жыл бұрын

    Watching them tear up the agar like that was a bit painful ngl

  • @hebahariri9936
    @hebahariri9936 Жыл бұрын

    Hello, I'm a microbiologist and a content creator on Instagram educating microbiologists in Arabic, can I translate your video and post it on my account? with sharing your video link of course. thank you.

  • @HardyDx

    @HardyDx

    Жыл бұрын

    Yes, that would be fine as long you tag and credit Hardy Diagnostics in your video description and link it back to the original video. Keep up the great work in microbiology.

  • @mtado4933
    @mtado4933Ай бұрын

    yeah i fucked it up so badly

  • @lilianmumbo880
    @lilianmumbo8802 жыл бұрын

    5 method of preparing specimen

  • @akritikuikel8506
    @akritikuikel85069 ай бұрын

    how to get a job or internship of microbiology in europe. I have done bachelor in microbiology and working in a restaurant since 4 years. Did not even get any inernship. Already forgot all the techniques and skills in these 4 years . sad sad

  • @BlueFox61

    @BlueFox61

    3 ай бұрын

    Build a portfolio of your interests and maybe your thesis paper too. It's hard to get an internship now

  • @user-me1hh5po6h
    @user-me1hh5po6h Жыл бұрын

    عربي وين

  • @Behejrbrbrjrj

    @Behejrbrbrjrj

    Жыл бұрын

    هنا موجودين 🙂🙂

  • @alvinxyz7419
    @alvinxyz7419 Жыл бұрын

    I thought the title said steak

  • @sriningsih4808
    @sriningsih4808 Жыл бұрын

    kangen haha

  • @John-fr6wo
    @John-fr6wo Жыл бұрын

    For God so loved the world that He gave His one & only Son, that whoever believes in him shall not perish but have eternal life. For God did not send His Son into the world to condemn the world, But to save the world through Him. (John 3:16-17)✝️

Келесі