In this video, we will explain the procedure for reading fluorescence in target organism colonies growing on HardyCHROM™ Candida + auris as an example. We will also name the common pitfalls in reading fluorescence.
To order any of the products mentioned in this video follow the links below:
HardyCHROM™ Candida + auris, Chromogenic Medium Cat. no. G343
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UV Lamp, E-Series, 1x 365nm 6 Watt BLB tube Cat. no. EA160
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UV Lamp, Handheld, 365nm, Rechargeable Battery + Charger Included Cat. no. LSS3
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Fluorescence Analysis Cabinet, Black-Out Viewing Box, Cat. no. CM10A
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Buffered Charcoal Yeast Extract (BCYE) Cat. no. G07
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Buffered Charcoal Yeast Extract (BCYE) Selective Agar with CAV
(anisomycin, colistin, and vancomycin) Antibiotics Cat. no. G08
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HardyCHROM™ Vibrio, Chromogenic Medium Cat. no. G319
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Nutrient Agar with MUG Cat. no. G114
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MUG Disks Cat. no. Z17
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EC Broth with MUG, 10ml Cat. no. K64
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CRITERION™ MacConkey Agar with MUG,
Dehydrated Culture Media, 2kg Bucket Cat. no. C6112
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00:00 Intro
00:43 To begin
00:55 Inoculate
01:23 Incubate
01:40 Observe
02:13 Check for fluorescence
02:36 Dark viewing box
02:52 UV flashlight
03:31 UV lamp
04:18 Caution
04:50 HardyCHROM™ Vibrio
05:26 BCYE
06:05 MUG products
6:45 Conclusion
07:21 Close and support