This has helped me a lot throughout my training. Thank you so much ❤ Keep uploading more stuff.

@MicroChemsExperiments

2 жыл бұрын

Glad it was helpful!

Very good ..well paced..accurate...professionally done..thx.

@MicroChemsExperiments

2 жыл бұрын

Thank you

Very informative content. Thank you ❤

Thanks for all your videos Your videos are always helpful Thanks 🙏🏻🙏🏻

@MicroChemsExperiments

3 жыл бұрын

LyRical WoRLD Sachin Yadav, you are most welcome. Thanks for being with us.

You make a very good explanation and love the details! I hope you can keep up this good channel!

@MicroChemsExperiments

2 жыл бұрын

Thank you Ma'am. Keep supporting me. From which country you are??

@hanazhafirahhanifah8175

2 жыл бұрын

@@MicroChemsExperiments I wish I could have known your channel when I was in MTLA Program 😁 I come from Indonesia but been 2 years in Germany. How about you? I was wondering if you can also make a video about "Plaque reduction neutralization test" and more bacteriology test including the biochemical test. It would be really helpful for us who cant bring the notes to another country 😊

@MicroChemsExperiments

2 жыл бұрын

@@hanazhafirahhanifah8175 Please knock on our Facebook page. Link is given in the description section.

@hanazhafirahhanifah8175

2 жыл бұрын

@@MicroChemsExperiments Will sure check it later at night! Thank you in advance!

@MicroChemsExperiments

2 жыл бұрын

@@hanazhafirahhanifah8175 You are welcome. Stay with us

This has helped me a lots during my PT

@MicroChemsExperiments

Жыл бұрын

Glad to know that

Thank you for this Explanation will help a lot on tomorrow's moke test and also for life time😌👌🏻

Superb art of work. Keep it up

@MicroChemsExperiments

2 жыл бұрын

Thank you very much. Stay with us.

Thanks for the vdo..the audio and vdo quality are really good

@MicroChemsExperiments

3 жыл бұрын

It's our pleasure

Thanks you for this information. I'm happy 😊 with you because I need this lesson

@MicroChemsExperiments

Жыл бұрын

Thank you

The video quality & background sound was awesome 💖

@MicroChemsExperiments

3 жыл бұрын

@Meftahul Jannat, Happy to hear that! Thank you so much. Be with us.

Thanks for sharing!!!

good job..... Thank you from Libya

@MicroChemsExperiments

2 жыл бұрын

Thanks for your support

Awesome.. very helpful. 🙏

@MicroChemsExperiments

2 жыл бұрын

Thank you.

Nice better way of teaching 👍

@MicroChemsExperiments

3 жыл бұрын

It's our pleasure. Thank you so much.

Oh yeah perfect thanks for this.

@MicroChemsExperiments

Жыл бұрын

Thanks

Great job 👌

@MicroChemsExperiments

2 жыл бұрын

Thanks

Very well explain 👌

@MicroChemsExperiments

Жыл бұрын

Thank you

I understand all things Thank you 👍👍👍👍👍👍👍👍👍👍👍🔥🔥🔥

@MicroChemsExperiments

Жыл бұрын

You are welcome!

Thank you a lot!

@MicroChemsExperiments

Жыл бұрын

You're welcome!

Good explanation

@MicroChemsExperiments

3 жыл бұрын

Thank you

The way of expression was SMART in details we will wait you....

@MicroChemsExperiments

3 жыл бұрын

Thank you so much for your support.

Very nice

@MicroChemsExperiments

2 жыл бұрын

Thank you

Hlo mem your explanation is very well

@MicroChemsExperiments

Жыл бұрын

Thank you. Stay with us

Wow nice

@MicroChemsExperiments

Жыл бұрын

Thanks

Thank you

@MicroChemsExperiments

3 жыл бұрын

Most welcome

Is this a Most Probable Number (MPN) method or Membrane Filtration Method (MFM)? Can it work for turbid water?

Vry vry use full tq so much ma'am

@MicroChemsExperiments

2 жыл бұрын

Thank you

@SriRam-cw8px

2 жыл бұрын

@@MicroChemsExperiments mam sds page podunga plzz Tamil la sollunga mam plzz

@MicroChemsExperiments

2 жыл бұрын

@@SriRam-cw8px Please write in English

@SriRam-cw8px

2 жыл бұрын

@@MicroChemsExperiments mam English avlova varathu Tamil sonna purenjipa so atha keta Tamil la podunganu

This is too much noise for me, background music

@MicroChemsExperiments

Жыл бұрын

Ok we will make it lower in the future

@mahabouyousoly

11 күн бұрын

Same remark

Please, what sample was used?

if we have a swab sample, how to prepare a sample?

after we strake , it fills the petri dish, not in scratches, is that contamination? Or too much bacteria?

I have a one question from you... I collected sample from the sewage water,and followed with 3 times serial dilution with bglb broth and incubated for the identification of coliform bacteria.after the production of gas I isolate them into(0.1ml) macconkey agar and did indole test for them for the confirmation.in plates I've got 60 colonies,so the CFU /ml was calculated...6x10^5 CFU /ml ...so,I need to dilute them into three samples.like 10 CFU/ml,100 CFU/ml and 1000 CFU/ml...so can u give me an idea how to do that.... And my second question after diluted them,I need to those cultures to grow inside the nutrient broth for the DNA extraction,so,how can I inoculate them,I mean after getting dilution,we should do the culture again which I mention in 1st question like culture into the agar plates,if I take the one colony from the agar plate define 1 ml?I mean let say we culture the agar plate from the 100cfu/ml sample if I take one colony from them means that colony contain 100cfu?

@MicroChemsExperiments

3 жыл бұрын

Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying.... Situation-1: You got 60 CFU from 0.1 BGLB broth culture ****Ans to the first question: 1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline. 2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline. 3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2. ****Ans to the second queation: A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate. Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate. If you dont get the expected result by following my guideline then try the bellow procedure: First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed. Thank you.

In this, you had prepared peptone salt solution, did you not use it in this entire procedure? Can you tell why was it prepared in this procedure?🤔

Hello dear. I have a question in cca agar plate how we can differentiate ecoli and coliform because cca is suitable for both e coli and coliform

sorry can you give the specifications of EMB AGAR MEDIA you used?

Hi, thank you for the video. May I ask for the peptone salt solution, can it be replaced with Buffered peptone water (BPW)? Also, I would like to ask is there a difference if I use 0.1% BPW, instead of BPW?

@MicroChemsExperiments

3 жыл бұрын

You can use BPW as diluent. No problem with it

@Wt520lyn

3 жыл бұрын

@@MicroChemsExperiments thank you

Thank you for the video. Is there a method for E coli enumeration?

@MicroChemsExperiments

3 жыл бұрын

Rbar H, You are most welcome. We will prepare video for the enumeration of E. coli soon. You can enumerate E. coli by following steps: 1. Dilute sample to appropriate dilution. 2. Spread 0.1ml diluted sample on CCA plate. 3. Consider purple colonies as presumptive E. coli. 4. Confirm E. coli colonies by growing on EMB and sMac plates. 5. Count confirmed colonies as CFU. To get the video, please wait. We will work on it soon.

@MicroChemsExperiments

3 жыл бұрын

Rbar H, please find the E. coli enumeration video. Video Link: kzread.info/dash/bejne/k3Z50bCektKneNY.html

❤️

@MicroChemsExperiments

2 жыл бұрын

Thanks

Could you tell what color colonies to check for salmonella, staphylococcus, pseudomonas with their respective agar medium. Also rest of the procedure would remain same ?

@MicroChemsExperiments

6 ай бұрын

We will make video soon

I have a doubt , for inoculation of E. coli on agar , we should use only one loopful of ecoli broth or for each quadrant we use individual loopful of ecoli

@MicroChemsExperiments

Жыл бұрын

You should use one loop full of E. Coli and draw the first quadrant. That's enough. No need to take again for other quadrant

Dear sir I want all microbiological tests videos for drinking water

@MicroChemsExperiments

2 жыл бұрын

Okay. Stay with us

In the procedure how to calculate the e coli?

I am a research student so can you please share the articles because I follow this video for the detection of E.coli. Please also explain what kind of the feed meal you use as a sample.

@MicroChemsExperiments

3 жыл бұрын

We have followed ISO 9308 & ISO 16649 to make this video. Please collect the handbook from ISO as we do not provide any pdf. In this video, we have tested a poultry feed meal sample.

@entertainmentpoint4242

3 жыл бұрын

@@MicroChemsExperiments Thank you so much.

@MicroChemsExperiments

3 жыл бұрын

@@entertainmentpoint4242 It's pleasure

Considering EMB and sMAC is used after 48 hours, is there need to prepare it on first day?

@MicroChemsExperiments

Ай бұрын

Not necessarily

For CCA, it don't need to be autoclaved before it is pour into the petri dish?

@MicroChemsExperiments

3 жыл бұрын

Siti Salihah Mohd Saidi, No. Because CCA is highly selective for E. coli and Coliform and high temperature (121 degree) may destroy the selective ingredients of the medium. That's why, no need to autoclave the medium during the media preparation. Just boil to dissolve the content and pour into petri dish.

@sitisalihahmohdsaidi2583

3 жыл бұрын

@@MicroChemsExperiments aaaa I see, thank you for the explanation

@MicroChemsExperiments

3 жыл бұрын

@@sitisalihahmohdsaidi2583 You are most welcome. Thanks for being with us.

Hello. I have a question. If we use this microbiological test for E.coli detection, do we also need to perform biochemical test?

@MicroChemsExperiments

2 жыл бұрын

Only perform Oxidase Test along with gram staining if you have facilities. Other biochemical test is not mandatory according to ISO reference method which is mentioned in this video.

@islaserena1599

2 жыл бұрын

@@MicroChemsExperiments Noted. Thank you so much !

@jaimahakal579

3 ай бұрын

Indole test can be performed?

if sorbitol agar we dont have so we use mackonkey agar ?

@MicroChemsExperiments

2 жыл бұрын

Yes. Use MacConkey Agar

Can I ask one question is this possible that your place is not cleab but still your coliform count is absent .

@MicroChemsExperiments

2 жыл бұрын

Yes. This is possible.

@foodiekamini299

2 жыл бұрын

@@MicroChemsExperiments how it is possible I am microbiologist I have done testing in certain sample where I have found colliform but the same sample when tested outside they didn't detect any coliform...can you plz sort out my quiery

Can we use this method for E.coli detection from water sample..

@MicroChemsExperiments

2 жыл бұрын

Yes. You can

Can we use this method for E.coli detection from hand?

@MicroChemsExperiments

2 жыл бұрын

You can use

What about double strength mac conky agar

@MicroChemsExperiments

2 жыл бұрын

We will make video later

thanks can i get pdf for this test,?

@MicroChemsExperiments

3 жыл бұрын

Bokul Hossain, we combined two ISO methods with few modifications for E.coli test to simplify and strongly confirm the E.coli cell. You can't match full method to any of the refetence methods.

E coli O157 Enrichment Medium Can buffered Peptone Wtaer with Pyruvate (bPWp) and ACV (Acriflavin-Cefsoludin-Vancomycin) be used ?????

@MicroChemsExperiments

2 жыл бұрын

Just use Selective media for E coli O157.

@ajitpatil2153

2 жыл бұрын

My Qus. pri-enrichment medium name ?? and Enrichment medium name?? which pri Enrichment and Enrichment medium use for detection of E coli o 157 H:7 test .??

@MicroChemsExperiments

2 жыл бұрын

@@ajitpatil2153 Enrichment Broth: mTSB with Novobiocin. Pre-enrichment is not necessary

@ajitpatil2153

2 жыл бұрын

Thanks

@MicroChemsExperiments

2 жыл бұрын

@@ajitpatil2153You are welcome

Please give calculation part

Biochemical tests

@MicroChemsExperiments

Жыл бұрын

No need as per ISO SOP.

Hi

@MicroChemsExperiments

2 жыл бұрын

Hello

Wich sample you give for the this test...plz tell me

@MicroChemsExperiments

3 жыл бұрын

Prajwal Nimbulkar, we tested a feed meal sample in this video

@prajwalnimbulkar267

3 жыл бұрын

@@MicroChemsExperiments thanks for replying Thankyou so much

@MicroChemsExperiments

3 жыл бұрын

@@prajwalnimbulkar267 Its pleasure. Stay with us.

How much tissue need for the purpose of study?😢

please what is the full meaning of CCA

@MicroChemsExperiments

22 күн бұрын

Chromocult Coliform Agar

Name of this type of method ????

@MicroChemsExperiments

3 жыл бұрын

The reference of the method: ISO 9308-1

How to detect second strain e coli O157 H:7 ????? plzz reply

@MicroChemsExperiments

2 жыл бұрын

Firstly, analyze you sample according to this video. If you get the E.coli, streak several bacterial colony on "HiCrome EC O157:H7 Selective Agar Base" selective agar plate. Finally if you find dark purple to magenta coloured colony then you can interpret your test result of e coli O157 H:7 as detected.

@ajitpatil2153

2 жыл бұрын

@@MicroChemsExperiments e coli o157 h:7 selective media name plzz ??

@MicroChemsExperiments

2 жыл бұрын

@@ajitpatil2153 Already mentioned in the first reply. The name is: HiCrome EC O157:H7 Selective Agar Base

@dinithiapsara7069

Жыл бұрын

Did u do this practical for o157 Ecoli

If you have maual for thia i want

@MicroChemsExperiments

2 жыл бұрын

Sorry. We don't provide any document

Please make video without music.

@MicroChemsExperiments

2 жыл бұрын

Okay

Where are u from mam ??

@MicroChemsExperiments

2 жыл бұрын

We should not discuss it. Please ask questions related to the video

Can we do internship here

@MicroChemsExperiments

2 жыл бұрын

No sir.

How to prepare the result and report to the given sample

@MicroChemsExperiments

3 жыл бұрын

Aanchal Gahalot, the result is E. coli detected. The the result should be reported as 'Detected/25g'

Is this biochemical test ?

@MicroChemsExperiments

2 жыл бұрын

No sir. This is Microbiological test

@hassaan7990

2 жыл бұрын

@@MicroChemsExperiments you have any video or PPT on identification of E.coli by biochemical testing . Because I am University student And I need this PPT for presentation

@MicroChemsExperiments

2 жыл бұрын

@@hassaan7990 No PPT is available. Search on Google

@hassaan7990

2 жыл бұрын

@@MicroChemsExperiments actually I searched it . But I could not find it. Can you please help me 🥺 for collecting the data .

@MicroChemsExperiments

2 жыл бұрын

@@hassaan7990 No sir. we can't help at this moment. Sorry

thankful for all video it is helpful am very interesting for more help

@MicroChemsExperiments

2 жыл бұрын

Thank you

Please give me the following information detection of E- coli o157 H:7 Pri- enrichment medium name -?? Enrichment medium name -??

@MicroChemsExperiments

2 жыл бұрын

Already mentioned