I haven't received notifications for your posts in a while. I missed you, your team, and your videos! Thank you! Welcome back!

@MicroChemsExperiments

2 жыл бұрын

So glad to hear this. Actually we were so busy in our professional life. Now, we are back. Stay with us.

Well explained thanks

Good video

Thanks for procedure

this I waited for long, thanks

@MicroChemsExperiments

2 жыл бұрын

I made it for you

merci pour la video

Sehr gut 👍

Excellet video

@MicroChemsExperiments

Жыл бұрын

Thank you very much!

Nice teaching mam

@MicroChemsExperiments

Жыл бұрын

It's my pleasure

Thanks for the excellent video. I have few questions 1. What can we use instead of stomacher bags ? 2. How to prepare sample if we have swab samples or environmental samples ? 3. Formula if we have n3 or n4 also ?

@MicroChemsExperiments

Жыл бұрын

1. Large duran bottle 2. Dissolve the swab stick in 10ml buffer solution. After shaking and vortex, dilute if necessary. Then analyze. 3. First calculate average from 2 plates. In this way, make 2 values from 4 plates.

thanks

@MicroChemsExperiments

Жыл бұрын

You are welcome

Can u please tell ........What is the formula for calculation if colony count in one successive dilution is less than 150?

👍

@MicroChemsExperiments

2 жыл бұрын

Thanks

Hi, is okay to use potato dextrose agar? with same procedure?

@kelvindanntobias5642

5 ай бұрын

Yes.

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

Kindly upload the Enumeration technique of Bacillus Coagulans

@MicroChemsExperiments

2 жыл бұрын

Noted

Please do the video of residue free chlorine

@MicroChemsExperiments

2 жыл бұрын

Noted

Please upload video for Staphylococcus aureus and Enterobacteriaceae count

@MicroChemsExperiments

Жыл бұрын

Noted

Hello. Does pour plating method can be used for yeast and mold culture?

@MicroChemsExperiments

Жыл бұрын

In that case you have to use SDA media for pour plate

Why we use diluent instead of water

Please can I use thesame technique and culture Media for pharmaceutical samples?

@MicroChemsExperiments

Жыл бұрын

Yes. You can

What is meant by BAM in the title?

@MicroChemsExperiments

2 жыл бұрын

Bacteriological Analytical Manual

Hi! Great video. Just want to ask did the sample weight change from 25g to 50g?

@MicroChemsExperiments

Жыл бұрын

The question is not clear. We took 25g sample for testing

@Ninxh678

Жыл бұрын

@@MicroChemsExperiments because according to the FDA-BAM Chp 1 the sample weight stated to be 50g. So I just want to ask did the result for 25g different from 50g?

@MicroChemsExperiments

Жыл бұрын

@@Ninxh678 Result will not differ.

@Ninxh678

Жыл бұрын

@@MicroChemsExperiments Thank you!

Can we use potato Dextrose Agar instead of DRBG Agar

@MicroChemsExperiments

Жыл бұрын

Yes you can but you need to add chloramphenicol as a supplement to make it more selective for Yeasts & Molds.

@ruqiyakhamroon4434

8 ай бұрын

R can we use potato Dextrose Agar w/chloramphenicol

Can you use buffered peptone water instead of 0.1% peptone water?

@MicroChemsExperiments

Жыл бұрын

You can

Is there any chemicals to prevent yeast and mould in packeged drinking water

@MicroChemsExperiments

Жыл бұрын

Yes. benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate are used to prevent the growth of yeast and mould

Hi, I am wondering what is the purpose of labeling 2 trial plats? eg: DRBC10-2 / trail -1 and DRBC10-2 /trail -2. Thanks in advance.

@MicroChemsExperiments

Жыл бұрын

Contamination can occur in every steps of any microbiological test. So double trial can increase your confidence level of the test.

Incubate plates in upright or invert position

@MicroChemsExperiments

5 ай бұрын

Invert

@ayakhaled2474

5 ай бұрын

But in iso method 21527-2 is written: 9.2.3 Incubate the prepared plates aerobically, lids uppermost, in an upright position in the incubator at 25 °C ± 1 °C for 5 d to 7 d. If necessary, leave the agar plates to stand in diffuse daylight for 1 d to 2 d.

Can we use blending jar instead of stomacher machine

@MicroChemsExperiments

Жыл бұрын

Contamination can occur in blender jar. So you can shake by your hand....or you can use vortex mixer

@gousiagulzar7002

Жыл бұрын

@@MicroChemsExperiments thank you

@MicroChemsExperiments

Жыл бұрын

@@gousiagulzar7002 welcome

Can we use Butterfield phospate buffer instead of peptone water

@MicroChemsExperiments

Жыл бұрын

Yes you can

@neethuaneethua941

Жыл бұрын

​@@MicroChemsExperiments thank you ❤

is there any video for bacterial count?

@MicroChemsExperiments

Жыл бұрын

Yes. Link: kzread.info/dash/bejne/e6CI0K5-l8zcpNY.html

Can we use this method for determination of yeast and mold?

@MicroChemsExperiments

Жыл бұрын

Yes

@sambathsocheata1389

Жыл бұрын

For enumeration need 6 falcon tube then how about determination do we still use 6 falcon tube or just use 2 tube only ?

@MicroChemsExperiments

Жыл бұрын

@@sambathsocheata1389 you can use only 2 tubes. It depends on the expected concentration of yeast and mold in your sample

@sambathsocheata1389

Жыл бұрын

Thank you ❤

Do you still need to add antibiotic solution in the media?

@MicroChemsExperiments

Жыл бұрын

No. No need to add antibiotics in DRBC agar media

@arlhenpinera1884

Жыл бұрын

@@MicroChemsExperiments thank youu

How to control yeast and mould in packeged drinking water

@MicroChemsExperiments

Жыл бұрын

Add one of these chemicals benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate.

@nainamohamed6727

Жыл бұрын

@@MicroChemsExperiments thank you for your kind reply one more doubt sir how much qty add per litre sir

Excuse me, can I get the reference of calculating formula?

@MicroChemsExperiments

Жыл бұрын

Reference is given in the video title

@sorngsinadane8447

Жыл бұрын

@@MicroChemsExperiments Many thanks to you. Keep this good work always!☺️

@MicroChemsExperiments

Жыл бұрын

@@sorngsinadane8447 Thank you so much.

what is the different about bacteria and yeast in Macroscopic ? I am still confused. Thank you

@MicroChemsExperiments

2 жыл бұрын

Study more in Google resources

one more issue media are not solidified

I haven't received any reply

@MicroChemsExperiments

Жыл бұрын

Sorry for late reply

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

@dennarkoe5928

Ай бұрын

26 degree for mould yeast with slowly incubation 5 day

Which temperature to set incubator 22-25° or 37° plzzz reply 😢

@Mmababy24

2 ай бұрын

37