Total Plate Count (Total Aerobic Bacterial Count)_A Complete Procedure (BAM, Ch-3)

Total Plate Count (Total Aerobic Bacterial Count) is a very important Microbiological testing parameter for Food, Feed, Water, Environmental and other samples analysis. This video represents a detailed procedure for the enumeration of total bacteria in a sample.
__________________________________________________________________________________________
Follow us on Social Networks to Stay Connected:
KZread: / @microchemsexperiments
Facebook Page: pg/MicroChem...
Be with us, be a Scientist. ______________________________________________________________________________________________
Music Credit:
Sound Cloud: / special-days-jayjen-au...
KZread Audio Library: • Roadtrip - Scandinavia... __________________________________________________________________________________________
Video Links:
Pour Plate Method: • Pour Plate Technique f...
Best Hygiene Practice: • Best Hygiene Practices...
__________________________________________________________________________________________
Related Tag:
#totalplatecount
#totalaerobicbacterialcount
#totalaerobicbacteria
#totalviablecount

Пікірлер: 146

  • @dasarisailaja-uv3lq
    @dasarisailaja-uv3lq7 ай бұрын

    Very good explanation thanks a lot

  • @SachinYadav-sk3fh
    @SachinYadav-sk3fh2 жыл бұрын

    Wow very useful video Thank you for your all the videos. Love you ❤️

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Thanks

  • @biannekaulf9221
    @biannekaulf9221 Жыл бұрын

    Wow, thank you very much for sharing this.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    You are welcome

  • @gousiagulzar7002
    @gousiagulzar700210 ай бұрын

    Your videos are saviour for people like me who are not from pure microbiology field 🥹

  • @MicroChemsExperiments

    @MicroChemsExperiments

    9 ай бұрын

    Thank you so much.

  • @efeodjegba4215
    @efeodjegba42152 ай бұрын

    this is amazing thank you

  • @officiallighthouse
    @officiallighthouse Жыл бұрын

    Excellent, very clear and to the point presentation.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Glad you liked. Thanks

  • @afolabipeter7073
    @afolabipeter70734 ай бұрын

    Am blessed to have watch this❤

  • @MicroChemsExperiments

    @MicroChemsExperiments

    4 ай бұрын

    Thank you

  • @deedee4527
    @deedee4527 Жыл бұрын

    Thank you so much, well explained.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Thank you

  • @mahnoorsohail8479
    @mahnoorsohail84799 ай бұрын

    best video 😍

  • @rakibhassan2451
    @rakibhassan24512 жыл бұрын

    Your video's always awesome 😘😘😘

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Thank you

  • @chauhanasmita4651
    @chauhanasmita46512 жыл бұрын

    It's really helpful thank you

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Thank you

  • @asmeretkidane4269
    @asmeretkidane42697 ай бұрын

    very nice presentation continue with clear

  • @ashapatil992
    @ashapatil992 Жыл бұрын

    Very nice procedure thank you

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Thank you

  • @KhalidSaleem-un8hd
    @KhalidSaleem-un8hdАй бұрын

    Wow it's Amazing and helpful❤

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Ай бұрын

    Thank you

  • @fathimasidhiky4412
    @fathimasidhiky44122 жыл бұрын

    very well explained

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Thank you

  • @hamidnazari2694
    @hamidnazari2694 Жыл бұрын

    Love you 😀🙃🙂😁😃 its so usefull

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Thanks

  • @balurajnadeesh2788
    @balurajnadeesh27883 ай бұрын

    Very useful vdo

  • @MicroChemsExperiments

    @MicroChemsExperiments

    3 ай бұрын

    Thank you

  • @abdallahadam9734
    @abdallahadam9734 Жыл бұрын

    I loved it

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Thanks

  • @user-xs2gk3yj9t
    @user-xs2gk3yj9t11 ай бұрын

    nice peresentation

  • @MicroChemsExperiments

    @MicroChemsExperiments

    11 ай бұрын

    Thanks

  • @paleshwongshrestha2798
    @paleshwongshrestha27982 жыл бұрын

    Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Noted

  • @purushotamgorasiya7109
    @purushotamgorasiya71092 жыл бұрын

    Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate

  • @user-bu1ki1sm5b
    @user-bu1ki1sm5b5 ай бұрын

    can you help me with what TPC count should be considered in acceptable range for frozen fruits and vegetables?

  • @HK-Media1
    @HK-Media16 ай бұрын

    Nice

  • @MicroChemsExperiments

    @MicroChemsExperiments

    6 ай бұрын

    Thank you

  • @mdsajibhossain747
    @mdsajibhossain7472 жыл бұрын

    Same methods can we use for Drinking water or juice analysis? One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water. Thank you very much.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Same method is for Drinking water or juice analysis. We will make video for yeast and Molds and Magnesium analysis

  • @zevanoc.sibarani8295
    @zevanoc.sibarani8295Ай бұрын

    Can you tell me what is the standard method you use to do this procedure? Thank you 🙏

  • @habibakhan3555

    @habibakhan3555

    8 күн бұрын

    Fda bam chapter 3 for Total aerobic count

  • @adzansaban3731
    @adzansaban37314 күн бұрын

    Sir, do you turn on the blower when working in the laminar?

  • @ervingopo5734
    @ervingopo5734 Жыл бұрын

    Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    To avoid condensation of water vapour on the lid of the dish because the media is still hot

  • @microbegist7918

    @microbegist7918

    Жыл бұрын

    I find this content helpful as a student doing her project.

  • @ervingopo5734
    @ervingopo5734 Жыл бұрын

    Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    To avoid condensation of water vapour on the lid of the dish because the media is still hot

  • @GMCLABORATORY
    @GMCLABORATORY6 ай бұрын

    Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?

  • @nankwopmarkus4884
    @nankwopmarkus48848 ай бұрын

    Why using phosphate buffer? Can one use distilled water instead?

  • @setiawanalwisius-tu6vx
    @setiawanalwisius-tu6vx Жыл бұрын

    Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.

  • @elsabeteyohannes6213
    @elsabeteyohannes621310 күн бұрын

    Thank you for your excellent explanation

  • @yosam9393
    @yosam93932 жыл бұрын

    sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Will make leter

  • @mani-ls7kj
    @mani-ls7kj2 жыл бұрын

    Please share the determination of ammonium acetate assay method

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Noted

  • @999_finesse
    @999_finesse2 жыл бұрын

    I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3 Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml The accepted count is

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit

  • @dessienatalia1193
    @dessienatalia11932 жыл бұрын

    Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Yes

  • @venkybunny1554
    @venkybunny15542 жыл бұрын

    would you please make a video on the hanna ph meter

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Already made. Link: kzread.info/dash/bejne/goasrMmmqsebmqg.html

  • @asmeretkidane4269
    @asmeretkidane42697 ай бұрын

    how many sample is done in the same time ?

  • @manjiripatwardhan336
    @manjiripatwardhan336 Жыл бұрын

    Can you please make video on MPN method for detection of Esheresia coli.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Noted

  • @meenageeths9730
    @meenageeths9730 Жыл бұрын

    Very easy planas

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Thanks

  • @akramasgari
    @akramasgari2 ай бұрын

    Best 👌

  • @juharAmeumare
    @juharAmeumare3 күн бұрын

    Adrenergic receptors activators

  • @juharAmeumare

    @juharAmeumare

    3 күн бұрын

    Adrenergic receptors activators

  • @rajuahmmed1257
    @rajuahmmed12572 жыл бұрын

    Could you please upload the quantification video of beta carotenoid from carrot sample by UHPLC?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Noted.

  • @zarqaarshad411
    @zarqaarshad4112 жыл бұрын

    Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Noted

  • @yen4310
    @yen4310 Жыл бұрын

    What if my acceptable counts is in the first dilution, will the formula be C1/1xd?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes. Please share your final results here

  • @kotaspecial9047
    @kotaspecial90472 жыл бұрын

    Can you guide me how to test tri basic lead sulphate testing method

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Will upload soon

  • @rjedey658
    @rjedey65811 ай бұрын

    Mam can I use this technique for khoaya,Chenna or any milk based sweets?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    11 ай бұрын

    Yes you can

  • @callistaemeali5052
    @callistaemeali5052 Жыл бұрын

    Can I use plate count agar and peptone water? Is that acceptable?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes. You can use.

  • @karthiyayanikrishnan5675
    @karthiyayanikrishnan5675 Жыл бұрын

    How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    11 ай бұрын

    Make report as

  • @gousiagulzar7002
    @gousiagulzar700210 ай бұрын

    Can we use peptone instead of buffer you mentioned

  • @MicroChemsExperiments

    @MicroChemsExperiments

    9 ай бұрын

    You can use peptone salt solution

  • @rutujajadhav9446
    @rutujajadhav9446 Жыл бұрын

    Which medium should be used total viable count of milk

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Plate Count Agar

  • @ynen_405
    @ynen_405 Жыл бұрын

    Can i use Nutrient agar and peptone water in cookies? Is it acceptable?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    You can use and will get results

  • @suzaava3691
    @suzaava36912 жыл бұрын

    In the formula, how did you get 1 and 0.1 please. Thank you

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    The formula is given by BAM, CHAPTER 3

  • @MrLongKR
    @MrLongKR5 ай бұрын

    hi. may i know the reference for this procedure?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    5 ай бұрын

    BAM, Chapter 3

  • @safeedhasaidalavi9786
    @safeedhasaidalavi9786 Жыл бұрын

    Can you pls tell the limit for total bacterial count in the case of biscuits

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    100CFU/g

  • @Madhuu151
    @Madhuu1512 жыл бұрын

    If there is a video that calculates the histamine level in a fish sample using the Elisa method, can you please provide the link?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    No video was made on this test yet.

  • @gargireghunath2114
    @gargireghunath2114 Жыл бұрын

    Is this method used for testing shrimp?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes, applicable

  • @khushiyadav6723
    @khushiyadav6723 Жыл бұрын

    Does the same method used in industries?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Yes. You can use

  • @neethuaneethua941
    @neethuaneethua941 Жыл бұрын

    I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks

  • @neethuaneethua941

    @neethuaneethua941

    Жыл бұрын

    ​@@MicroChemsExperimentsthank you

  • @neethuaneethua941

    @neethuaneethua941

    Жыл бұрын

    Can you please upload a video about enrerobacteriacea (IS 17112 part ll

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    @@neethuaneethua941 noted

  • @neethuaneethua941

    @neethuaneethua941

    Жыл бұрын

    I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.

  • @karntokaali9778
    @karntokaali9778 Жыл бұрын

    Can I replaced by nutrient agar media?????

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    You can but without any reference

  • @DESIVOCALTV
    @DESIVOCALTV Жыл бұрын

    Why we make two/more petri plates of same dilution ?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates

  • @IzzyRoxy
    @IzzyRoxy2 ай бұрын

    Very clear explanation, really helped me alot thankssss 🥹💖

  • @saurabhdeshmukh2605
    @saurabhdeshmukh2605 Жыл бұрын

    Of which sample you had calculated this cfu

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Soyabean meal

  • @vikashganvit3548
    @vikashganvit3548 Жыл бұрын

    Ma'am how to do antimicrobial activity of polymer films?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Same as shown in the video

  • @vikashganvit3548

    @vikashganvit3548

    Жыл бұрын

    @@MicroChemsExperiments polymeric film is solid

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    @@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole

  • @sashikalawanasinghe1021
    @sashikalawanasinghe10212 ай бұрын

    Can this procedure use to butter sample?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 ай бұрын

    No

  • @sashikalawanasinghe1021

    @sashikalawanasinghe1021

    Ай бұрын

    What is the procedure used to butter sample ? 😢

  • @bulakochebo7636
    @bulakochebo7636 Жыл бұрын

    please whose protocol is it?

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Reference Method is already mentioned. Please check again

  • @sonal3124
    @sonal31242 жыл бұрын

    Which method use? Pls send the method name

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    Reference is given in this video

  • @degontoislam925
    @degontoislam9252 жыл бұрын

    could you please share the reference for this work, thank you

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    BAM chapter 3

  • @degontoislam925

    @degontoislam925

    Жыл бұрын

    @@MicroChemsExperiments thank you

  • @anbarulhaque4720
    @anbarulhaque47202 жыл бұрын

    3.21 cfu/g is my reading .: Then how many colonies are there

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    There are about 3 colonies in 1g of your sample.

  • @anbarulhaque4720
    @anbarulhaque47202 жыл бұрын

    How to convert cfu/g to cfu/ml

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample

  • @ankitsaxena8738
    @ankitsaxena87387 ай бұрын

    Ky ap ye video hindi me nhi bna skte hain aur 🙏❤️

  • @megharaghuwanshi4102
    @megharaghuwanshi4102 Жыл бұрын

    How to perform B.et test

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Please write the full name of the test

  • @somnathchanda1250
    @somnathchanda1250 Жыл бұрын

    Use natural accent

  • @MicroChemsExperiments

    @MicroChemsExperiments

    Жыл бұрын

    Ok. Will try

  • @dr.shashankbaghel
    @dr.shashankbaghel3 ай бұрын

    thanks but you should avoid fake accent

  • @anbarulhaque4720
    @anbarulhaque47202 жыл бұрын

    3.21 cfu/g is my reading .: Then how many colonies are there

  • @MicroChemsExperiments

    @MicroChemsExperiments

    2 жыл бұрын

    There are about 3 colonies in 1g of your sample.

Келесі