Total Plate Count (Total Aerobic Bacterial Count)_A Complete Procedure (BAM, Ch-3)
Total Plate Count (Total Aerobic Bacterial Count) is a very important Microbiological testing parameter for Food, Feed, Water, Environmental and other samples analysis. This video represents a detailed procedure for the enumeration of total bacteria in a sample.
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Пікірлер: 146
Very good explanation thanks a lot
Wow very useful video Thank you for your all the videos. Love you ❤️
@MicroChemsExperiments
2 жыл бұрын
Thanks
Wow, thank you very much for sharing this.
@MicroChemsExperiments
Жыл бұрын
You are welcome
Your videos are saviour for people like me who are not from pure microbiology field 🥹
@MicroChemsExperiments
9 ай бұрын
Thank you so much.
this is amazing thank you
Excellent, very clear and to the point presentation.
@MicroChemsExperiments
Жыл бұрын
Glad you liked. Thanks
Am blessed to have watch this❤
@MicroChemsExperiments
4 ай бұрын
Thank you
Thank you so much, well explained.
@MicroChemsExperiments
Жыл бұрын
Thank you
best video 😍
Your video's always awesome 😘😘😘
@MicroChemsExperiments
2 жыл бұрын
Thank you
It's really helpful thank you
@MicroChemsExperiments
2 жыл бұрын
Thank you
very nice presentation continue with clear
Very nice procedure thank you
@MicroChemsExperiments
Жыл бұрын
Thank you
Wow it's Amazing and helpful❤
@MicroChemsExperiments
Ай бұрын
Thank you
very well explained
@MicroChemsExperiments
2 жыл бұрын
Thank you
Love you 😀🙃🙂😁😃 its so usefull
@MicroChemsExperiments
Жыл бұрын
Thanks
Very useful vdo
@MicroChemsExperiments
3 ай бұрын
Thank you
I loved it
@MicroChemsExperiments
Жыл бұрын
Thanks
nice peresentation
@MicroChemsExperiments
11 ай бұрын
Thanks
Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder
@MicroChemsExperiments
2 жыл бұрын
Noted
Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?
@MicroChemsExperiments
2 жыл бұрын
Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate
can you help me with what TPC count should be considered in acceptable range for frozen fruits and vegetables?
Nice
@MicroChemsExperiments
6 ай бұрын
Thank you
Same methods can we use for Drinking water or juice analysis? One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water. Thank you very much.
@MicroChemsExperiments
2 жыл бұрын
Same method is for Drinking water or juice analysis. We will make video for yeast and Molds and Magnesium analysis
Can you tell me what is the standard method you use to do this procedure? Thank you 🙏
@habibakhan3555
8 күн бұрын
Fda bam chapter 3 for Total aerobic count
Sir, do you turn on the blower when working in the laminar?
Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?
@MicroChemsExperiments
Жыл бұрын
To avoid condensation of water vapour on the lid of the dish because the media is still hot
@microbegist7918
Жыл бұрын
I find this content helpful as a student doing her project.
Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.
@MicroChemsExperiments
Жыл бұрын
To avoid condensation of water vapour on the lid of the dish because the media is still hot
Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?
Why using phosphate buffer? Can one use distilled water instead?
Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?
@MicroChemsExperiments
Жыл бұрын
There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.
Thank you for your excellent explanation
sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?
@MicroChemsExperiments
2 жыл бұрын
Will make leter
Please share the determination of ammonium acetate assay method
@MicroChemsExperiments
2 жыл бұрын
Noted
I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3 Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml The accepted count is
@MicroChemsExperiments
2 жыл бұрын
There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit
Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?
@MicroChemsExperiments
2 жыл бұрын
Yes
would you please make a video on the hanna ph meter
@MicroChemsExperiments
2 жыл бұрын
Already made. Link: kzread.info/dash/bejne/goasrMmmqsebmqg.html
how many sample is done in the same time ?
Can you please make video on MPN method for detection of Esheresia coli.
@MicroChemsExperiments
Жыл бұрын
Noted
Very easy planas
@MicroChemsExperiments
Жыл бұрын
Thanks
Best 👌
Adrenergic receptors activators
@juharAmeumare
3 күн бұрын
Adrenergic receptors activators
Could you please upload the quantification video of beta carotenoid from carrot sample by UHPLC?
@MicroChemsExperiments
2 жыл бұрын
Noted.
Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths
@MicroChemsExperiments
2 жыл бұрын
Noted
What if my acceptable counts is in the first dilution, will the formula be C1/1xd?
@MicroChemsExperiments
Жыл бұрын
Yes. Please share your final results here
Can you guide me how to test tri basic lead sulphate testing method
@MicroChemsExperiments
2 жыл бұрын
Will upload soon
Mam can I use this technique for khoaya,Chenna or any milk based sweets?
@MicroChemsExperiments
11 ай бұрын
Yes you can
Can I use plate count agar and peptone water? Is that acceptable?
@MicroChemsExperiments
Жыл бұрын
Yes. You can use.
How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?
@MicroChemsExperiments
11 ай бұрын
Make report as
Can we use peptone instead of buffer you mentioned
@MicroChemsExperiments
9 ай бұрын
You can use peptone salt solution
Which medium should be used total viable count of milk
@MicroChemsExperiments
Жыл бұрын
Plate Count Agar
Can i use Nutrient agar and peptone water in cookies? Is it acceptable?
@MicroChemsExperiments
Жыл бұрын
You can use and will get results
In the formula, how did you get 1 and 0.1 please. Thank you
@MicroChemsExperiments
2 жыл бұрын
The formula is given by BAM, CHAPTER 3
hi. may i know the reference for this procedure?
@MicroChemsExperiments
5 ай бұрын
BAM, Chapter 3
Can you pls tell the limit for total bacterial count in the case of biscuits
@MicroChemsExperiments
Жыл бұрын
100CFU/g
If there is a video that calculates the histamine level in a fish sample using the Elisa method, can you please provide the link?
@MicroChemsExperiments
2 жыл бұрын
No video was made on this test yet.
Is this method used for testing shrimp?
@MicroChemsExperiments
Жыл бұрын
Yes, applicable
Does the same method used in industries?
@MicroChemsExperiments
Жыл бұрын
Yes. You can use
I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.
@MicroChemsExperiments
Жыл бұрын
Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks
@neethuaneethua941
Жыл бұрын
@@MicroChemsExperimentsthank you
@neethuaneethua941
Жыл бұрын
Can you please upload a video about enrerobacteriacea (IS 17112 part ll
@MicroChemsExperiments
Жыл бұрын
@@neethuaneethua941 noted
@neethuaneethua941
Жыл бұрын
I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.
Can I replaced by nutrient agar media?????
@MicroChemsExperiments
Жыл бұрын
You can but without any reference
Why we make two/more petri plates of same dilution ?
@MicroChemsExperiments
Жыл бұрын
There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates
Very clear explanation, really helped me alot thankssss 🥹💖
Of which sample you had calculated this cfu
@MicroChemsExperiments
Жыл бұрын
Soyabean meal
Ma'am how to do antimicrobial activity of polymer films?
@MicroChemsExperiments
Жыл бұрын
Same as shown in the video
@vikashganvit3548
Жыл бұрын
@@MicroChemsExperiments polymeric film is solid
@MicroChemsExperiments
Жыл бұрын
@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole
Can this procedure use to butter sample?
@MicroChemsExperiments
2 ай бұрын
No
@sashikalawanasinghe1021
Ай бұрын
What is the procedure used to butter sample ? 😢
please whose protocol is it?
@MicroChemsExperiments
Жыл бұрын
Reference Method is already mentioned. Please check again
Which method use? Pls send the method name
@MicroChemsExperiments
2 жыл бұрын
Reference is given in this video
could you please share the reference for this work, thank you
@MicroChemsExperiments
2 жыл бұрын
BAM chapter 3
@degontoislam925
Жыл бұрын
@@MicroChemsExperiments thank you
3.21 cfu/g is my reading .: Then how many colonies are there
@MicroChemsExperiments
2 жыл бұрын
There are about 3 colonies in 1g of your sample.
How to convert cfu/g to cfu/ml
@MicroChemsExperiments
2 жыл бұрын
These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample
Ky ap ye video hindi me nhi bna skte hain aur 🙏❤️
How to perform B.et test
@MicroChemsExperiments
Жыл бұрын
Please write the full name of the test
Use natural accent
@MicroChemsExperiments
Жыл бұрын
Ok. Will try
thanks but you should avoid fake accent
3.21 cfu/g is my reading .: Then how many colonies are there
@MicroChemsExperiments
2 жыл бұрын
There are about 3 colonies in 1g of your sample.