We have to take pre-incubated plates pf XLD or BSA?

Very on point and interesting video.

@MicroChemsExperiments

10 ай бұрын

Much appreciated

Thank you so much, this video was very helpful

@MicroChemsExperiments

3 жыл бұрын

Thank you. Stay with us

Thank you for uploading this video.. and it's really helpful and understandable too.. please do video for detection of staphylococcus spp and vibrio spp.

@MicroChemsExperiments

2 жыл бұрын

Thank you. We will make video later.

Thank you so much. I try to find part 2 but cannot find. Please..

Please,give us the link for part 2 and do the video for bacillus cereus

Thank u so much mam ...its very informative

@MicroChemsExperiments

2 жыл бұрын

You are welcome

Excellent explanation thanks 💐👍

@MicroChemsExperiments

2 жыл бұрын

Thank you

Good job, I've got a cultured plate with distinct colonies and I'll need your help in identifying the bacterium and medium used. Thank you

@MicroChemsExperiments

3 жыл бұрын

Its a long task. Its difficult to explain here. Neverthless you can subculture your colonies on different selective & differential culture media like MSA, Cetrimide Agar, XLD, SS agar, Chromogenic Coliform Agar, MRS, MYP agar, etc. After that do biochemical tests of the selected colonies for identification.

Tomorrow i wll face auditor in micro lab its very helpful to me thnkyo so muchh

@MicroChemsExperiments

Жыл бұрын

All the bestttt

@khushichaturvedi3817

Жыл бұрын

@@MicroChemsExperiments i will try to my best

Very info .thanks

@MicroChemsExperiments

3 жыл бұрын

It's pleasure.

Good job

@MicroChemsExperiments

2 жыл бұрын

Thanks

Hi! I just wanna ask if it is necessary to use both RVS and MKTTn for selective enrichment? as well as XLD and BSA for Isolation? thank you so much

@MicroChemsExperiments

Жыл бұрын

Yes. Both needed for selective broth. And you can use only XLD for isolation

Thanks a lot make more videos for E. Coli, and zeldahl test protein testing and show serial dilution also

@MicroChemsExperiments

3 жыл бұрын

Okay. We will make it. Stay with us.

Thanks for this video ! I couldn't find Part 2, are you going to upload it ? Thanks !

@MicroChemsExperiments

3 жыл бұрын

Already uploaded. Link: kzread.info/dash/bejne/k5qktaZ9psetfcY.html

This vedio help me alot

@MicroChemsExperiments

3 жыл бұрын

Thank you. Stay with us.

Very useful

@MicroChemsExperiments

Жыл бұрын

Thank you

lot of thanks

@MicroChemsExperiments

3 жыл бұрын

It's our pleasure...

hi your video is very resourceful but can we use selenite broth instead of RVS and MKTTn broth? and also another question, what is the name of this method used? thank you

@MicroChemsExperiments

11 ай бұрын

Yes you can. Method reference is mentioned in the video title

Thank you....

@MicroChemsExperiments

3 жыл бұрын

You're welcome!

Very informative. Can I use distilled instead of peptone water?

@MicroChemsExperiments

2 жыл бұрын

No. Peptone water is used for Non-selective enrichment. Non-selective enrichment can not be achieved by distilled water. So you must use Peptone Water.

thank a lot 😍

@MicroChemsExperiments

3 жыл бұрын

Most welome

Waiting for part 2.

@MicroChemsExperiments

3 жыл бұрын

We will upload soon

Mam what sample do you use?

Please make a video on Bacillus cereus as per ISO 7932:2004 and Staphylococci aureus as per ISO 6888-2:1999 If possible.

@MicroChemsExperiments

3 жыл бұрын

Ok we will work on it soon.

Thank u so much❤

@MicroChemsExperiments

11 ай бұрын

You're welcome 😊

What are the advantages and disadvantages of this method as compared to others detection methods?

@MicroChemsExperiments

2 жыл бұрын

1. Advantage: This method involves double enrichment steps which increases detection possibility and facilitates to detect Salmonella more selectively, thus this is the more accurate method compared with other methods. 2. Disadvantage: MKTTN media, frequently, becomes not available in local market and there is no replacement media of it.

Thanks! Is part 1 already uploaded?

@MicroChemsExperiments

2 жыл бұрын

Both parts are already uploaded.

Thank youuuu

@MicroChemsExperiments

2 жыл бұрын

My pleasure

Well done my teacher. Please can I use this method for pharmaceutical samples?

@MicroChemsExperiments

Жыл бұрын

Yes. You can

With the exception of RVS/MKTT, any proposed salmonella enrichment broth? Thank you

@MicroChemsExperiments

2 жыл бұрын

Not at all

@abhijitmukherjee441

Жыл бұрын

Salanite broth

Do you have video on testing of pseudomonas Aureginosal. Please share

@MicroChemsExperiments

2 ай бұрын

We will make it soon

Do you have a video for preparing xld agar plate? Because xld agar can not autoclave

@MicroChemsExperiments

2 жыл бұрын

We will recheck

@SnoooooowMan

Жыл бұрын

Do you have a xld agar plate preparing? I don't know why my xld agar always in a liquid

@fariyahnaseem5184

2 ай бұрын

Don't cover the lid of plate until medium is solidified N make sure the agar is completely dissolved in Duran bottle while heating in microwave This can be achieved when u see the medium is boiling clearly i.e bubbles formation.

Wauu. This is really a good video. Can anyone share with me a full document where I can find medium types used to enumerate bacteria founds in food. Please help if possible. Have a nice weekend

@MicroChemsExperiments

2 жыл бұрын

We can't provide any document. We are sorry. Thanks for your appreciation

@fariyahnaseem5184

2 ай бұрын

Plz read bam manual

Can you name simpler tests for detection of Salmonella with close accuracy?

@MicroChemsExperiments

2 жыл бұрын

No other simpler method to be known with close accuracy.

Excuse me, may I ask? What type of another sample that is easy to detect salmonella ?

@MicroChemsExperiments

Жыл бұрын

Test poultry feces, that has the high possibility to detect salmonella

Is it compulsory to use all the reagents mentioned?

@MicroChemsExperiments

Жыл бұрын

Yes

Ma'am plz make a video on yeast and mould determination

@MicroChemsExperiments

2 жыл бұрын

Noted.

Why does solid foods need 24hrs for incubation after homogenized with buffered peptone water???..Not only for salmonella detection but also for enumeration of TPC??

@MicroChemsExperiments

Жыл бұрын

24 hours incubation in buffered peptone water is needed for the enrichment of salmonella. But this enrichment is not applicable for TABC or TPC

@istiakmostofa7167

Жыл бұрын

@@MicroChemsExperiments then what to do for TPC after homogenized with buffered peptone water???

@MicroChemsExperiments

Жыл бұрын

@@istiakmostofa7167 kzread.info/dash/bejne/e6CI0K5-l8zcpNY.html

Do we need to make 225ml of BPW in the non-selective isolation stage? Why not less than that?

Pls why did u use 1ml of peptone in mkTTn broth and 0.1ml in RVS broth

@MicroChemsExperiments

Жыл бұрын

Method said so to do that for the selective enrichment of Salmonella spp.

Mam, what kind of sample you r using?

@MicroChemsExperiments

2 ай бұрын

Animal feed sample

Can we substitute XLD with SS agar

@MicroChemsExperiments

2 жыл бұрын

Yes. You can.

What sample did you use??

@MicroChemsExperiments

Жыл бұрын

Food Sample

Can I prepare buffer peptone water from peptone

@MicroChemsExperiments

2 жыл бұрын

You can prepare according to the composition of Buffered Peptone Water.

Can please confirm 6:16 mints its 0.1 ml or 100ml its confusing

@MicroChemsExperiments

Жыл бұрын

0.1ml (100 microliter)

What ois the name of the sample taken for the experiment?

@MicroChemsExperiments

10 ай бұрын

Poultry Feed Sample

how much broth we have to take in the test tube?

@MicroChemsExperiments

3 ай бұрын

10 ml

@user-bu1ki1sm5b

3 ай бұрын

i dont have a stomachercan i use blender?@@MicroChemsExperiments

What was the sample used?

@MicroChemsExperiments

3 жыл бұрын

Animal feed

2:42 what sample is that??

@MicroChemsExperiments

Жыл бұрын

food

Part 2?

@MicroChemsExperiments

Жыл бұрын

kzread.info/dash/bejne/k5qktaZ9psetfcY.html

Refrence?

@MicroChemsExperiments

2 ай бұрын

Iso 6579

E-coli o157 plzz selective Agar name

@MicroChemsExperiments

2 жыл бұрын

HiCrome EC O157:H7 Selective Agar Base, Himedia

@ajitpatil2153

2 жыл бұрын

@@MicroChemsExperiments thank you dear

@MicroChemsExperiments

2 жыл бұрын

@@ajitpatil2153 You are most welcome

@ajitpatil2153

2 жыл бұрын

@@MicroChemsExperiments hicrome coliform Agar ???????

Good video but the method is time consuming.

@MicroChemsExperiments

11 ай бұрын

Yes, you are right

name of sample ,?

@MicroChemsExperiments

3 жыл бұрын

Animal Feed

Everything is okay, except the narration. The speech is not clear. Please work upon that. Rest, I love your channel.

@MicroChemsExperiments

2 жыл бұрын

Thank you for your observations. We will try to improve.