We can learn this with a sentence Come In And Stain C- Crystal violet I - iodine A- alcohol ( ethanol) S- safranin

@hollywoodisasataniccult4022

4 жыл бұрын

Pooja Pareek yo thank you!! Have my micro practical tomorrow. Im a Pharm d student in Pakistan 😊😊

@adnanahmed7463

4 жыл бұрын

Thank you for helping me remember this:)

@nocmemer4686

4 жыл бұрын

Pooja di thx,I have exams tomorrow it will help me

@poojapareek1386

4 жыл бұрын

@@nocmemer4686 all the best for your exam dear😊...and no need of thanks dear I'll be great full if I can help you 😊😊

@nocmemer4686

4 жыл бұрын

So sweet of you😇

Interesting! Never had to do this before, kinda wish I did. Only disappointment was at the end because I was kind of expecting to see what that slide looked like under the microscope.

@antonyshigin4977

2 жыл бұрын

Yeah...Me too disappointed......

@ProfRoofs

Жыл бұрын

Here you go ---> kzread.info/dash/bejne/l5ukvJJ7lK23dZs.html

@nadaahmed6236

Жыл бұрын

@@ProfRoofs THANK YOU SO MUCHHH

this is one of the best representations of a gram staining procedure I have every seen. Simple and straight forward with all the precautions built it. Well done.

thank you for this. i hope i'll do well on my practicals tomorrow 😨

@gargimahajan2273

7 жыл бұрын

ᄏᄏ아라 So, how did it go?

@firephoenix8192

6 жыл бұрын

I have praticals tomorrow as well

@iqrailyas2097

6 жыл бұрын

i have practical tomorrow too

@hollistercal888

5 жыл бұрын

same lol

@rvsingh4538

5 жыл бұрын

Mine too

Staining is to improve the contrast within a cell to show the organelles in greater clarity and detail. The cytosol is transparent there is little if any contrast. Using positively charged dye it attracts to the cytosol within the cell and you can distinguish between the different organelles. Using a negatively charged dye it repels the outside of the cell and stays outside the cell and distinguishes the cell from the background. The positive dye could be Methylene blue and the negative dye could be Congo red. Differential staining is used to differentiate between two different organelles or organisms. Gram stain technique is used to show gam positive bacteria and gram negative bacteria whilst the acid fast technique is used to show mycobacterium form other bacteria present.

Thank you for making this video! My lab partner hogs all of the lab activities and doesn't care about other people, so I could not learn it well sadly. There is a lab exam on Gram staining, so I am glad that you made this video so I can learn how to do it!

I have microbiology exam next week. This video is so helpful for students of medical schools like me. Thank you for making it👏

Nice video! I wish I had seen this *before* doing Gram Stain for the first time as a high school student. Instead, I had to rely on an ambiguously worded worksheet for instructions. I ended up washing my slide completely clean of all cells... That was spectacular, I suppose. ah ha ha :)

@stookiegrace1253

6 жыл бұрын

Joules Kin same bit somehow my e. Coli cane out gram-positive which was correct!

@sajanvincent1111

3 жыл бұрын

Thank u for the information

I have a gram stain lab tomorrow and this was really helpful!!

I have my practical exams tomorrow And today I am watching 😂😂 So thankful to you for explaining each and every step perfectly Hope I will do well tomorrow 😂😘❤️😂

@A9poj

2 жыл бұрын

armyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyy

@_Dr.P_

2 жыл бұрын

@@A9poj ARMY 💜💜💜💜

@himanshurao8668

10 ай бұрын

@@A9pojstupid armyyy

nooo i wanna see the results :'(

@srija_7890

2 ай бұрын

😭

@KevinRaizada

Ай бұрын

Me too

@arian138

Ай бұрын

Thank you for saving my time, not to see the video. You lost but you saved many.

quick and straight to the point explanation

Awesome. Passionate to perform this on lab.

So gram staining is to differentiate between gram positive and gram negative bacteria and acid fast technique is to differentiate mycobacterium from other bacteria

@JessM20_

7 жыл бұрын

Kathryn Russell yup!

@firozahmad1653

4 жыл бұрын

Acid fast different iate Mycobacterium tuberculosis. And m. leprae

Thank you for this! you saved me from the embarrassment of asking my lab partner who likes to gatekeep information to himself

Thanks I've understood the technique properly thanks to this video.

My histology exam after one day! This vdu was so informative thanks ✨

Thank you helped me for my bacteriology subj

Ugh I hate lab practicals. These KZread vids are a godsend!

Thank you so much, I learnt a lot from this video!

@Cccvgd13

2 жыл бұрын

Thank you so much ,I learnt alot from this video!

Oh! This is the greater Graim Strainig procedure I've ever seen. It's so interesting.

Thank you for making this video!

This is amazing, so interesting, I like it!

I did it yesterday in my internship. I'm so happy.

thanks that was SO helpful 💖👏👩‍🔬

thankyou, its all cleared. having exam of Gram staining tomorrow 🙌🏼

Very clear explanation keep it up sir

Interesting and helpful episodes.

That was so interesting I am not a science student but it was like I can understand everything by watching this video

Can you post all your lab experiments...lol I learned so much through this THANKS!!1

@Kidsent567

5 жыл бұрын

If it is time i will post it dr

simple and easy, thank you.😊😊😊

Its wonderful understand the Subject after this video saved me Thanks very much

Where is the observation?

nic and straight technique

Very clear and informative tutorial.

@sulimankhan621

5 жыл бұрын

good

tank you for this good presentations.i got it good experiance.

Thank you so much for this video. ❤

Amazing video

Wow so amazing your explain 😍

Thanks 😊 that was so helpful ☺

I have a gram stain skill exam in a few days…can’t wait lol

Rinsing in a beaker seems a lot more controlled than using spray bottles as they seem to do in the lab I'm taking classes in.

Thanks that was helpful ☺

very useful video.tommorw my practical xam.

Post more helpful videos on equipments and related solutions which are used and how can they be used

Great video 🫶🏻

Thanks a lot, would really be helpful in my defense

Thank you for this useful video

@BioRadLaboratoriesCorp

3 жыл бұрын

You're welcome! If there are basic biology and life-science laboratory techniques you'd like to learn more about, email the Bio-Rad Explorer education program at explorer@bio-rad.com

Good job 👍

Thanks that was helpful

Thank you . Is very interesting

Good job

this is interesting to watch goodjob 🎉

thank you for this..i hope i'll write the same in my exam👍👍

Tnks for this usedful video.....

Thank you very much

we wash the slide with a bit of detergent before use to make it grease free which helps alot in spreading the water droplet and giving a smear of bigger surface area.........idk if it's right but it's a mandatory step in our university and it has become a part of staining procedure

Helpful...

loved it except for the smear. it was a little too cloudy in my opinion.

@erikhernandez6450

7 жыл бұрын

i think it may have been agar residue

wish me luk on my practical :)

Useful one

Nice video

Interesting your videos good amazing

Thank you for this uploading vedio

So awesome thnku so much

Thank u soooo much ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

Thankuu so much it really help me thankss.

Tqs good information sir

How come when I apply the alcohol most of my specimens come out red, even something like gram positive bacillus subtilis comes out half red half blue and sometimes completely red. Professor said that could happen if I'm applying too much alcohol but I do it as instructed, until it runs clear. Could it be that my school has such old specimens that all the peptidoglycan is long gone and alcohol just washes everything away?

@VanillaSnake21

5 жыл бұрын

I can answer this now that I've finished the semester, by the end I was able to do perfect Gram stains. The reason it could sometimes comes out mixed color or the blue color washes off completely is mostly due to uneven alcohol coverage. You have to place the drop of alcohol all the way at the top of the slide and let it slide down on it's own, also don't follow the directions "until it runs clear" once you see that it's light blue it's fine to stop. It's just a matter of getting the amount and the coverage right. By the way, got an A+ in this class, due in part to videos such as these. Many thanks.

The video was nice, but I have a question, u marked a circle with wax pencil , and made a smear on the same side???? As I'm a new student, my proff taught me to mark the slide , invert it n prepare a smear on inverted slide.... Why y did so?????😶

i understand this video is 10 years old, but i just want to be sure. is the gram decolouriser Ethanol solution? because i'm sure that's what we used for our gram staining methods to rinse the stain, but maybe i am just mixing up the wrong reagents

Better than my professor explanation

thank you

i'm just a random person who thought that gram-negative means something about the weight thank you for education!

If the stain of crystal violet came inti my hand how do i take it off

Great video although you could've shown what the samples looked like after all of those steps.

What are the other counter stains other than safranin can be used ?

IT'S A VERY GOOD VEDIO

Ooo very difficult but i sure , i will do in this time 😊

Perfect

haha I was waiting for what it looked like under the microscope

I'm here because one of my character is a scientist and I wanted to describe part of the procedure for Gram straining!

Tq sm sirr 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏

I interasting for your information

👌👌👌 ghaint

Thanks

Thanks good

Jeez man, did you flame the loop enough?

Interesting

Nice

how long does it take to perform and analyze a gram stain ?

@heyitsrayrui

2 жыл бұрын

Like 15-20 mins?

at very fast if you add drop of water to bacterial colony, the cells may swell and lyse due to osmotic gradient. better use a drop of isotonic normal saline

what if u accidentally swipe the bacteria from the wax pencil circle?

Good

thank u

wowwww it's so amazing yeah

Full prepared before 1 hours exam❤😁

Excellent video. Just one addition, Safranine being a weak stain should be kept for 2 mins

@AngelStickman

2 жыл бұрын

From my current work experience, one minute is sufficient.

@khgnew763

2 жыл бұрын

over staining may turn gram positive to negative

@swapnilpradhan507

2 жыл бұрын

@@khgnew763 No, its not that way, Gram positives will not take up counter stain

Shouldn’t the alcohol be continuously added, drop by drop on the smear for 30-45 seconds?

I have praticals tomorrow omg