Drosophila Media Preparation
Ғылым және технология
Nancy Levensailor and Danielle Hindes from the Ferguson lab at SUNY Fredonia demonstrate how to make Drosophila Media.
- Required Equipment
- 20 quart Groen Steam Kettle (TDH-20 is shown but electric models are also available. Look on E-bay for used kettles.)
- Drosofiller (59-168 Genesee Scientific)
- Narrow vial trays for "Reload System" (59-207GRE Genesee Scientific)
- Power drill. Any model will do. This is an inexpensive alternative to "lab mixers".
- Drywall mixer. (Home Depot)
- 1 L Pyrex measuring cup for corn syrup. (Grocery store)
- Microwave
- Autoclave
- 2 and 4 L plastic beakers. (1201-2000 & 1201-4000 from Fisher Scientific)
- Silicone spatula (Grocery store)
- 5 gallon buckets with lids for storing dry ingredients (Home Depot). This prevents bugs from contaminating the ingredients.
- Cotton bandanas (Walmart)
- Ingredients
- Drosophila Agar (66-103 Genesee Scientific)
- Corn Meal (Campus food service)
- Soy Flour (Campus food service)
- Deactivated dry yeast (Campus food service)
- Corn Syrup (Campus food service)
- Propionic Acid (AC14930-0025 Fisher Scientific)
- Consumables
- Narrow polystyrene vials for "Reload System" (32-109RL Genesee Scientific)
- Large cotton balls for vials (22-286-247 Fisher Scientific)
- 8 oz. polypropylene bottle (AS-117 Fisher Scientific)
- Bottle flugs (AS-277 Genesee Scientific)
Protocol scaled from the standard Bloomington Stock Center recipe at flystocks.bio.indiana.edu/Fly_...
Пікірлер: 7
I find your lack of PPE disturbing.
Hey...i had a doubt...the yeast you add ....does it get completely degraded due to heat?...or else it might cause some infection
wow, thanks for posting this. is the propionic acid being used as a preservative here? also, how are you controlling the PH? I would have thought there would be some variation between each batch of ingredients that would need to be corrected for?
How long does this food keep when stored?
can we use yeast extract instead of yeast?
@fredoniaferguson
7 жыл бұрын
Yes. We use yeast extract.
The fact that they have no PPE ..... they even wear open toed sandles ...... is an awful example to others. I would pull the video and remake with proper safety equipment.