Scott Ferguson

Scott Ferguson

Recording #4

Recording #4

Ferg MBoD Presentation

Ferg MBoD Presentation

Fredonia Leica SP8 Confocal

Fredonia Leica SP8 Confocal

Drosophila Egg Chamber

Drosophila Egg Chamber

Mitochondria depth coded

Mitochondria depth coded

Drosophila Ovary Dissection

Drosophila Ovary Dissection

Drosophila Media Preparation

Drosophila Media Preparation

Пікірлер

  • @rahulkrishna8602
    @rahulkrishna86026 ай бұрын

    Well well, there is some propaganda behind this experiment that is placed intentionally to fail students, Hail VRD

  • @zaki.102
    @zaki.1026 ай бұрын

    For real, never once got it and the lecturer gets mad at us for no reason like it's not my fault I don't disect larva on a daily 😢

  • @_Armycarat_
    @_Armycarat_6 ай бұрын

    Exactly.....There is only 10 percent out of 100 to get polytene successfully....😢hail VRD😉

  • @techiearj7909
    @techiearj79096 ай бұрын

    Apple

  • @akemikang9681
    @akemikang96819 ай бұрын

    I swear to god i cried so hard while doing this, my prof didn't explain anything properly and screamed at all of us for not getting polytene chromosome :( thanks your video helped me a lot, now i will try it tomorrow

  • @user-tu8vh3gp6c
    @user-tu8vh3gp6c10 ай бұрын

    murder !

  • @dr.atiyakousaryunus7542
    @dr.atiyakousaryunus7542 Жыл бұрын

    1:23 👏👏👏👏👏👏💐

  • @dr.atiyakousaryunus7542
    @dr.atiyakousaryunus7542 Жыл бұрын

    Good attempt bro

  • @athishmarutharaj6001
    @athishmarutharaj6001 Жыл бұрын

    Delicious

  • @michaeladdis3323
    @michaeladdis33232 жыл бұрын

    Injection pressure?

  • @geethanjallistudio9457
    @geethanjallistudio94572 жыл бұрын

    Wow it's a great job

  • @lolasdm6959
    @lolasdm69592 жыл бұрын

    ouch

  • @fadildo
    @fadildo8 ай бұрын

    yeeeeeouch

  • @abhrajyotinandi8546
    @abhrajyotinandi85463 жыл бұрын

    Hey...i had a doubt...the yeast you add ....does it get completely degraded due to heat?...or else it might cause some infection

  • @wenliu9703
    @wenliu97033 жыл бұрын

    amazing!

  • @Fordance100
    @Fordance1004 жыл бұрын

    Nice and quick

  • @user-dl4rt8mh5k
    @user-dl4rt8mh5k4 жыл бұрын

    I'm in an online class because of COVID19, and I need a Drosophila salivary gland dissection in my experiment. Can I use this video? It will only be used for experimental classes.

  • @laurapurkey2326
    @laurapurkey23264 жыл бұрын

    you make this look a lot easier than it is lol nice video !

  • @strongsoul9028
    @strongsoul90284 жыл бұрын

    Wooooow....Thank YoU..!...

  • @Bokchoy836
    @Bokchoy8364 жыл бұрын

    God the drosophila was still alive...

  • @dennisdrobeck8772
    @dennisdrobeck87724 жыл бұрын

    The fact that they have no PPE ..... they even wear open toed sandles ...... is an awful example to others. I would pull the video and remake with proper safety equipment.

  • @lindsaybachman720
    @lindsaybachman7204 жыл бұрын

    What is the harm in anesthetizing them first? I do not appreciate your approach.

  • @oliverojeda4234
    @oliverojeda4234 Жыл бұрын

    you let her know lindsay

  • @frogiafrog2132
    @frogiafrog21324 жыл бұрын

    What am I wattching

  • @ashrafpangaden7027
    @ashrafpangaden70275 жыл бұрын

    how you fabricated glass capillary

  • @harshitharajshekar2234
    @harshitharajshekar22345 жыл бұрын

    In masters we are unable to get this , nice video

  • @MechaKoops
    @MechaKoops5 жыл бұрын

    Shoutout to Dr. Ferguson

  • @sebandersenwood
    @sebandersenwood6 жыл бұрын

    wow, thanks for posting this. is the propionic acid being used as a preservative here? also, how are you controlling the PH? I would have thought there would be some variation between each batch of ingredients that would need to be corrected for?

  • @breannanowak8538
    @breannanowak85387 жыл бұрын

    How long does this food keep when stored?

  • @Genenat
    @Genenat7 жыл бұрын

    Why don't you behead it so it won't suffer so long.

  • @sorhsee
    @sorhsee5 жыл бұрын

    bruh....it's been dead for a while.

  • @Szabarmawi
    @Szabarmawi7 жыл бұрын

    Oh my God.. this is much easier than holding 2 needles and pull em apart.. thank you

  • @tovichish8075
    @tovichish80755 жыл бұрын

    Yea true..😂

  • @kritikasood3463
    @kritikasood34638 жыл бұрын

    can we use yeast extract instead of yeast?

  • @fredoniaferguson
    @fredoniaferguson8 жыл бұрын

    Yes. We use yeast extract.

  • @HanniieRawrr
    @HanniieRawrr8 жыл бұрын

    I find your lack of PPE disturbing.

  • @garethzhuo8006
    @garethzhuo80068 жыл бұрын

    brutal \m/

  • @kartikyevarshney1754
    @kartikyevarshney17548 жыл бұрын

    Thanks a lot for posting this. Tried this for the first time today..killed almost 7 without satisfactory result. Now I understand it better.

  • @zainabqureshi9178
    @zainabqureshi91788 ай бұрын

    Heyh

  • @HaileyMorganful
    @HaileyMorganful9 жыл бұрын

    That seems so cruel to me to stretch a living animal until it breaks in half. Couldn't you have killed it first? I'm not sure if a fruit fly larva can feel pain but that did NOT look fun no matter how small he was

  • @Gangularis
    @Gangularis8 жыл бұрын

    Many animals in nature wish for such a fast death. This was a fucking maggot!!!

  • @lajoswinkler
    @lajoswinkler7 жыл бұрын

    It does not feel pain and it has no ability to process that pain into suffering. It's a larva, not a bunny or a cow.

  • @Jessiecoonjames
    @Jessiecoonjames6 жыл бұрын

    fuck you

  • @interstellish
    @interstellish3 жыл бұрын

    I'd love for you to see the alternate universe of what people would be doing to humans these days for therapy in medicine if we did not have the insight that we've gained via working on things like fruit fly larvae.

  • @free_range_fruit
    @free_range_fruit3 жыл бұрын

    @@lajoswinkler I see all life as valuable, from a maggot to a human. If I can avoid their suffering in any way, I'm going to do it.

  • @murdemo
    @murdemo11 жыл бұрын

    Dude you helped me alot! thank you very much!!

  • @murdemo
    @murdemo11 жыл бұрын

    HI! How can i make a calibration curve log pb vs rf with this software??

  • @dusterbia
    @dusterbia11 жыл бұрын

    What kind of tweezers are those?? I mean they're really small

  • @interstellish
    @interstellish3 жыл бұрын

    look like number 5 forceps to me

  • @mikkelobi8761
    @mikkelobi876111 жыл бұрын

    This is interesting Dr. Ferguson! I will also be dissecting the ovaries of Pseudacteon phorid flies, smaller than Drosophila, in the next few days to study their fecundity, have you dissected any of such small flies?

  • @Mark-co1qw
    @Mark-co1qw2 жыл бұрын

    lmao

  • @scytheslash
    @scytheslash11 жыл бұрын

    Hi, that was very infomative. Do you have any suggestions, as to how i can shake out mature eggs from a clumped mass of ovarioles? Say by using a special media formulation and agitation by pipetting? I need to assay for number of eggs in fly ovaries and takes a long time to manually sit and tease all the eggs apart. Thanks!

  • @dieutran9066
    @dieutran906611 жыл бұрын

    Good guy. Just only 1 hit, u get the glands. Thank you. It's very helpful for me

  • @fantasia030
    @fantasia03011 жыл бұрын

    This was extremely helpful. thank you! I did that for the first time today but my larva were much more aggressive! How did you get it to stay in a straight line once you get a hold of it from one side. mine ATTACKED the forceps holding it.

  • @kanid.290
    @kanid.2904 жыл бұрын

    Hahahahha XD

  • @bhu2011
    @bhu201112 жыл бұрын

    ossmm gr8 job....ek bar me hi u took out the salivary gand...gr8 yar

  • @pointguard12
    @pointguard1212 жыл бұрын

    Very well done. Took me many tries to become proficient at this in my lab.

  • @fredoniaferguson
    @fredoniaferguson13 жыл бұрын

    We just do the dissection in PBS.

  • @emkesslerkc
    @emkesslerkc13 жыл бұрын

    Thanks for posting this. It increased my students level of success, and I'll be introducing students to it again this coming week.