Now this was the best explanation I have had . After watching multiple videos still I reached here to get more detailed information Thanks for this !!

@animatedbiologywitharpan

4 жыл бұрын

Hassa Iftikhar please share among your friends and don’t forget to check out all my playlists

The video is great! Thank you very much! I assume it is in fact an in vivo method not in vitro. Otherwise, you wouldn't have needed all this yeast cell machinery to perform the assay.

Informative...thanku

Thank you for make this topic easy. 😊 waiting for many more like this.

@animatedbiologywitharpan

Жыл бұрын

Follow me on instagram and facebook My social media handle is @animatedbiologywitharpan

Great video. Could you try making a video on translation and transcriptional inhibitors. A summary of mechanism with a few examples of toxins and antibiotics

@animatedbiologywitharpan

5 жыл бұрын

ya ya it is requested by many people ,so I would be doing that soon

your explanation is good, though I have got a doubt. Y2H is a in-vivo method or in-vitro method? you mentioned this technique as in-vitro. please clarify which is correct

@animatedbiologywitharpan

Жыл бұрын

What is invivo vs in vitro is highly contextual. In convenientional sense it should be in vitro

@biochemistryiseasy

Жыл бұрын

​@@animatedbiologywitharpan as it occurs in yeast hence invivo

very nicely done. explained it in the easiest way possible

@animatedbiologywitharpan

9 ай бұрын

Please share my channel link with your friends

Yeast two-hybrid (Y2H) screening is an in vivo method not vitro

Very good video ! Thank you !

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Great video, thank you so much!

@animatedbiologywitharpan

5 жыл бұрын

Share among your friends and help them as well

@MrsHarryPotterFan97

5 жыл бұрын

@@animatedbiologywitharpan I'll definitely do it! keep up the good work

good video, I do have a question though. Why do GAL AD and GAL BD bind, when two protein interact? Coulnt the active sites interacting with eath other from the bait and the fish protein be on the opposite sides of those of the GAL proteins, which would mean that the protein interact but the GAL proteins are to far from each other to interact and activate the reporter gene? Or on the other hand isnt it possible as well to have two proteins which dont interact but the the GAL proteins still are able to bind and produce the reporter genes? Shoundt there be a large number of false postive and false negatives with this method?

@animatedbiologywitharpan

4 жыл бұрын

It’s a very interesting question.... honestly I don’t know a definite answer... I would check the structural basis of interaction and get back to you

You are really awesome I really admire you😊..thnks for making each topic so easy to understand. 🎉

@animatedbiologywitharpan

Жыл бұрын

Please help me by sharing my contents with your friends

@animatedbiologywitharpan

Жыл бұрын

By the way revise your concepts using our mcq prep course only at 350 rs one time pay with life time subscription. animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef

Thnku so much sir fr explaining this cncpt in easy way🥰🙏🙏

@animatedbiologywitharpan

2 жыл бұрын

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@Kaur_2343

2 жыл бұрын

@@animatedbiologywitharpan sure sir😊

Thank u somuch for the video👏😍.. I have a doubt regarding transcription factor binding to protein , I think bait protein binds to DNA binding domain of transcription factor and fish/prey protein with that of activation domain..can u plz give a clarification?

@animatedbiologywitharpan

3 жыл бұрын

Anek dhonyobad Bandhu….Bangladeshi Khaoar amar khub valo lage….Poddar ilish er kono tulona nai….

Great explanation! Many thanks!

@animatedbiologywitharpan

Жыл бұрын

Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

@animatedbiologywitharpan

Жыл бұрын

Please share my channel link with your friends and help me to reach big audiance

You are my life saver 😊😊

@animatedbiologywitharpan

8 ай бұрын

please share my channel link with your friends

I saw the video well. But I have a question. 1:41 Isn't it correct to combine AD with Fish protein and DBD with Bait protein? Please understand what you say with a translator because you don't know English.

Very good one. Sir how to check the interactions through multiple dropout media say histidine, uracil trp and so

@animatedbiologywitharpan

2 жыл бұрын

Thanks susmita for your suggestion, I will try to make a video on that. It just that I get very less time to make videos

Very well explained.👏👌

@animatedbiologywitharpan

10 ай бұрын

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GREAT VIDEO! YOU SIMPLIFIED IT. PLEASE IS THIS RELATED TO YEAST TRANSFORMATION . PLEASE EXPLAIN

@animatedbiologywitharpan

4 жыл бұрын

Transformation means delivering gene into a cell .....it is possible to do it on bacteria ,yeast or mammalian cell culture. So generally for yeast electroporation is the mode of gene delevery

@animatedbiologywitharpan

4 жыл бұрын

Any way if you like my video please share my channel link with your friends and help me to reach big audience

veryyy clearrr thank youu

@animatedbiologywitharpan

2 жыл бұрын

Please please share my channel link with your friends and help me to reach big audience

Excellent 😍😍😍😍

@animatedbiologywitharpan

3 жыл бұрын

Please share among friends and help me to reach big audience

I really don't like long videos, and you just explained in 5 mins thank you sir... My exam is a day after tomorrow 😂 and I'm here try to understand ...

@animatedbiologywitharpan

3 жыл бұрын

Please check out other playlist for more such videos....share with your friends as well

Is there a promoter expressing gal4ad? What initiates the gal4ad to bind to the dna?

@animatedbiologywitharpan

Жыл бұрын

Yes yes a minimal promoter is there.

I am new in the molecular field

Nice explanation using animation

@animatedbiologywitharpan

7 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

seriously, all the reactions are happening inside the cells under physiological condition. how is this method in vitro instead of in vivo? doesn't make any sense.

Succinct and well-explained. Thank you for your service. ~a masters student

@animatedbiologywitharpan

Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

@animatedbiologywitharpan

Жыл бұрын

Please dont forget to share with friends

Sir can you please make a video on Nanobodies as a tool for protein structure and function studies Waiting for this 🙏

@animatedbiologywitharpan

Жыл бұрын

Honestly i don't have expertise on that

@Edu-Tech952

Жыл бұрын

@@animatedbiologywitharpan ok sir thanks for your rply❤️

Chrome Says It's An in Vivo Technique

Sr kya aap kisi onlyn platform pe teach krte ho?? Like unaccdmy ???

@animatedbiologywitharpan

2 жыл бұрын

Yes I have courses in unacademy. For more info email me at arpanparichha1994@gmail.com

@Kaur_2343

2 жыл бұрын

@@animatedbiologywitharpan ok sr

Isn’t it an in vivo technique?

@animatedbiologywitharpan

Жыл бұрын

Depends what you call in vivo. Some people belive in vivo is tissue level stuff. For Some it is cell level stuff.

I have heard that this is an in vivo method as this happens within the yeast. Isn't it so?

@animatedbiologywitharpan

4 жыл бұрын

Just because it is happening in an yeast it's not in vivo...the terms are used in pretty loose manner . ( for example cell culture is also considered as in vitro models )

@anaghamenon7857

4 жыл бұрын

@@animatedbiologywitharpan Thank you for the clarification!

@animatedbiologywitharpan

4 жыл бұрын

Please subscribe to my other channel kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html

@jiaqimao8620

3 жыл бұрын

​@@anaghamenon7857 Literally, what he has explained to you is not correct. Cell culture is considered to be in vitro because such method is only used to produce biological compounds such as antibodies, instead of to detect a reaction or interaction inside the cells. The majority of the experiment is performed outside of the cells. Yeast 2 hybrid system is an in vivo technique because not only all the proteins are expressed and matured inside the cells, but also the interaction. So, what you have heard before is correct!

use pointer to point "this", otherwise very easy to understand :)

@animatedbiologywitharpan

2 жыл бұрын

Tha js for the suggestion.....I will incorporate it in my subsequent videos

Great video! Could I use it for a university web project? I cannot see any details about copyrights, that's why I'm asking :)

@animatedbiologywitharpan

3 жыл бұрын

Use it with acknowledgement

@dvsiia

3 жыл бұрын

@@animatedbiologywitharpan Thank you! :)

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Some say its done in vivo

@animatedbiologywitharpan

Жыл бұрын

Ya depends on what you consider as in vivo

@dishaganguly8106

Жыл бұрын

Library based screening large scale of binary interaction shows in vitro which is phage display and in vivo is Y2H

I think it's in vivo not in vitro

@animatedbiologywitharpan

2 жыл бұрын

Its a controversy ! One school of thought suggests its in vivo, because the interaction is tested inside an organism . But current view conflicts that. Any way for undergrad textbook level, lets consider it in vivo.

This is wrong, it is an in vivo method of studying PPIs.

Video is good but your voice is speed. I am not able to follow. Please speak slow.

@animatedbiologywitharpan

4 жыл бұрын

Thanks for the feedback... but you can always change the playback speed to 1.5 x for making it slower.... may be you can try it

@animatedbiologywitharpan

4 жыл бұрын

Please share among your friends and help me to reach big audience

But this is an in-vivo technique. Also, I guess you explained the Bait & Fish concept incorrectly. Here is the reference: - www.ncbi.nlm.nih.gov/pmc/articles/PMC2705515/