If you are using magnetic beads, you don't need to do centrifugation. it would be separated by a magnet only. In the case of agarose beads, you would need centrifugation.

6 in-depth lecture slides couldn't help with what you did in 4 minutes! Thank you so much.

@animatedbiologywitharpan

4 жыл бұрын

Nova Roemer please support me by sharing my channel link with your friends and juniors

@animatedbiologywitharpan

4 жыл бұрын

Nova Roemer do watch my other playlists , as I have variety of contents which may be useful

BEST EXPLANATION I'VE GOTTEN! THANK YOU!

@animatedbiologywitharpan

6 жыл бұрын

glad to know it helped you.....share with friends and help them as well....happy learning

@sadafbahadori1453

4 жыл бұрын

That was great

You save me! Really thank you Your video is precious!!

@animatedbiologywitharpan

4 жыл бұрын

if you share my channel link with your friends or college group ....many people can get help and it would help me to reach big audiance

Lovely diagrams and nice clear explanation!

Can you please explain how beta actin will be present in the lysates when we only pulled our protein of interest with the beads, all other proteins will be in the supernatant which we will discard or not use for this particular gel. We will run only those proteins which are in pellet and i think beta actin wont be presnt in pellet?

Great video! Super clear and easy to follow... I also liked your diagrams. Thanks for sharing

@animatedbiologywitharpan

5 жыл бұрын

Thanks.... good to know that it helped...now share among friends to help them as well

hello! thank you very much for posting these explanations on youtube. just watched the long term potentiation (LTP) and this one about CO-IP and it helped me a lot! just subscribed :)

@animatedbiologywitharpan

4 жыл бұрын

Please share among your friends and help me to reach big audiance

Very good explanation, thank you so much, and the diagrams are so neatly-drawn too! Was so confused about co-IP and IP before this haha. Thank you!

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Thank you so much for the clear explanation! You saved my course!!!!

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

This was PERFECT! Thank you! Also you have very nice handwriting!

@animatedbiologywitharpan

4 жыл бұрын

please share among friends and help me to reach big audiance

@allienikole18

4 жыл бұрын

@@animatedbiologywitharpan absolutely!

thanks a lot! from Argentina!!

@animatedbiologywitharpan

5 жыл бұрын

Glad to hear that my video helped..... share among friends as well

The best explanation l have found. Thank you

@animatedbiologywitharpan

4 жыл бұрын

Fatn McHollandl please share and subscribe to my channel

Thank you so much for this clear explanation!

@animatedbiologywitharpan

5 жыл бұрын

Glad to hear that you found it useful

thank you brother!

Thanks a lot. your videos are really helpful for young researchers.

@animatedbiologywitharpan

3 жыл бұрын

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a very straightforward video ! thanks a lot !

@animatedbiologywitharpan

2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Explained very clearly thank you!

@animatedbiologywitharpan

3 жыл бұрын

Please share with your friends and help me to reach big audiance

Love your hand drawing!

@animatedbiologywitharpan

4 жыл бұрын

Please share among your friends and help me to reach big audience

If we dont know which protein is interacted with Protein A then how we can use the antibody against the unknown protein. In this case we can only go for sequencing the SDS PAGE bands interacted with Protein A

@Echodonut

5 жыл бұрын

You would have to go with mass spectrometry though. Sequencing is used on genetic material, not on proteins.

good job brah...keep uploading

Lourd la vidéo, ça m'a mis bien 👍

YOU SIR ARE GENIUS !

@animatedbiologywitharpan

3 жыл бұрын

Thanks a lot for the complement. Unfortunately I am not able to reach a big audience somehow...please help me by sharing my channel link with your friends.

@animatedbiologywitharpan

3 жыл бұрын

Check out all my playlist...you would definitely find topics of your interest

thanks this is short, concise and logical, made it easy to understand

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Im a medical student,Thank you so much for this video❤️❤️❤️❤️it helps a lott!

@animatedbiologywitharpan

3 жыл бұрын

Please share among friends and help me to reach big audience

@animatedbiologywitharpan

3 жыл бұрын

Do checkout my other playlists

Co-immunoprecipitation (Co-IP) is a popular technique to identify physiologically relevant protein-protein interactions by using target protein-specific antibodies to indirectly capture proteins that are bound to a specific target protein.

OH MY GOD THIS IS SO GOOD!!!!!!!!!!!!!!!!!!!! THANK YOU!!!!!!!!!!!!!

@animatedbiologywitharpan

2 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@fatcammal

2 ай бұрын

@@animatedbiologywitharpan anytime i search up biology content, this girl "bumbling biochemist" pops up. her stupidly long videos monopolize biology content, I swear, every single topic i search up i see her face.

very helpful video, the only video explains co-IP with western blot!

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Thank you man, it will facilitate my presentation. You are the best :))

@animatedbiologywitharpan

4 жыл бұрын

Please support me by sharing my channel link with your friends and help me to reach big audience

Very nice presentation

Very useful and excellent explanation. Thanks a lot 👌

@animatedbiologywitharpan

Жыл бұрын

Please share my channel link with your friends and help me to reach big audiance. Don't forget to subscribe

Thanks

I don't understand why we need a second antibody for protein B! Would it not show anyway on the SDS Page? Or did I miss something?!

The difference between Pull-down and CoIp is that pull-down using solution from eluted beads, and CoIp uses the precipitate after centrifuge. Then both of them go to SDS-PAGE, is that right?

Thanks for explanation! It was really helpful

@animatedbiologywitharpan

Жыл бұрын

Pleaae share my channel link with your friends and help me to reach big audiance

how do you justify the pH of the buffer to make sure that protein A and B are not dissociated?

It's fantastic thanks!

@animatedbiologywitharpan

2 жыл бұрын

Most welcome! Please share with your friends to help my channel grow...😇

Thank you for this video.

@animatedbiologywitharpan

2 жыл бұрын

Most welcome! Please share with your friends to help my channel grow...😇

Super useful, thank you !!

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

thank you!

@animatedbiologywitharpan

3 жыл бұрын

Please share among friends and help me to reach big audience

So helpful, thanks!

@animatedbiologywitharpan

4 жыл бұрын

Don’t forget to checkout my other playlists

Do you pull down beta actin too with the beads? How do you get beta actin in an IP blot?

Nicely explained explained. Could have been better if the importance of running input in the same gel was also given.

@animatedbiologywitharpan

3 жыл бұрын

I agree with your suggestion.

thank you

@animatedbiologywitharpan

4 жыл бұрын

Please share among your friends

Thank you, sir :)

@animatedbiologywitharpan

3 жыл бұрын

Please share among friends and help me to reach big audience

4:53 how will you get any B-actin band from an immunoprecipitated sample? if b-actin is present this simply means that b-actin is also interacting with the protein A, which is WRONG!

@tsamchoe1638

4 жыл бұрын

very true.

@CancerSleuth

4 жыл бұрын

Use IgG heavy chain as a loading control or simply immunoblot [WB] the precipitating protein (here A) to observe that protein A is present in equal amount in all the lanes.

actin is used just to make sure the proteins you are testing presence for were loaded correctly on the blot?

@animatedbiologywitharpan

3 жыл бұрын

Actin is a housekeeping protein so it’s not expected to change

Thanks for the very nice video. But I have a question. How can we detect unknown protein bound to the know protein in co immunoprecipitation ?

@animatedbiologywitharpan

3 жыл бұрын

Atleast you need to have one known protein which you would pull down and look for interactors via mass spec

Helpful 👌

@animatedbiologywitharpan

4 жыл бұрын

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THANKS! NOW I CAN UNDERSTAND A PAPER THAT I HAVE TO READ xD

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Where is protein A on the blot? How is A not blotted?

@jacobi_official8590

7 жыл бұрын

the target was B. thats why the antibody spotted just B in the western blot. A is there but the antibody wasn't directed against it. a positive band for B (considering that A was pulled down initially) shows there is interaction between A and B

r u from Presidency? very good keep up the good work bro..👍

@animatedbiologywitharpan

7 жыл бұрын

I am from TIFR

Do you have this information from a source? If so, which one? Can you send me the link please

@thomasprovoost7119

3 жыл бұрын

You liked my reaction, but you don't answer?

How we select antibody for a protein, How we can sure that our antibody interact with our protein??

@animatedbiologywitharpan

Жыл бұрын

kzread.info/dash/bejne/h2tqrs5sf9ioo7g.html

tell me are u from India? what is that accent??? i need to know

@animatedbiologywitharpan

2 жыл бұрын

Yes I am Indian, sorry if my accent has bothered you.

you save me!

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

does this only work for covalent interactions?

@animatedbiologywitharpan

2 жыл бұрын

No no generally protein protein interactions are non covalent.

great explanation

@animatedbiologywitharpan

4 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@animatedbiologywitharpan

4 жыл бұрын

Do explore my other playlists and you might find something very useful

Sir it is in vitro technique????

@animatedbiologywitharpan

4 жыл бұрын

Could be used for in vitro or in vivo

What is the purpose of using beta actin in this assay sir?

@animatedbiologywitharpan

4 жыл бұрын

It's a house keeping control......you can imagine it to be standard scale

Amazing explanation, however, isn't this an in-vitro technique? Literally everything is happening outside of the body, in a test tube etc. Thanks, nonetheless.

@animatedbiologywitharpan

4 жыл бұрын

Nope ...you got it wrong..... this method can be used to detect both in vitro and in vivo protein protein interaction....

@animatedbiologywitharpan

4 жыл бұрын

When you are doing from a cell lysate or tissue homogenate then it’s trying to detect the interactions in vivo, while you are doing with purified protein in test tube it’s in vitro....

@animatedbiologywitharpan

4 жыл бұрын

The interaction between proteins could be either in vivo or in vitro but a technique is never in vitro / invivo.....I hope this answers your doubt.....

@usernamenishta-4411

4 жыл бұрын

@@animatedbiologywitharpan ohhh okay. Yes that clears the confusion. Thankyouu 😊😊

@usernamenishta-4411

4 жыл бұрын

@@animatedbiologywitharpan ive been taught something so different 😂😂😂 and ive a paper in about an hour.

so by this way you describe yo identify the complex AB not their interaction as say !!!

good presetation, except some accent, but it's good. thanks.

Who teaches better? A. Your professor majored in biotechnology with 10+ years teaching experience Or B. A random indian boi on youtube

@animatedbiologywitharpan

3 жыл бұрын

It depends on the audiance

QuickQuestion You are studying 3 proteins (A, B and C). You hypothesize that A binds to B (forming a complex AB) but not C. From the scientific literature you know that B does not interact with C. 1) You are able to obtain 100% pure preparations of proteins A, B and C. Which experimental technique would you use to prove/disprove your hypothesis? Describe the experiment and the expected results

@animatedbiologywitharpan

2 жыл бұрын

Email me at arpanparichha1994@gmail.com. we can interact further

what is this accent ?

@animatedbiologywitharpan

6 жыл бұрын

fakeha khan this is Bengali accent....sorry for that