Unbelievable i have seen my 4y ago comment here when i was in my Bachelor and now im watching it again for my exam 😅 and guess what im in my phd now 😂..

@animatedbiologywitharpan

8 ай бұрын

😀😃😃😃😃

One of the best videos on elisa in youtube, thanks

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

It's good video for better understanding ELISA

You've explained the whole thing in a good manner. Thank you.

@animatedbiologywitharpan

6 күн бұрын

@@md.rifatkhandaker5876 Here is the link for competative elisa kzread.info/dash/bejne/aop90qWwhJysiNo.htmlsi=6mKNoqmduXEnIn6C

@animatedbiologywitharpan

6 күн бұрын

@@md.rifatkhandaker5876 here is sandwich elisa kzread.info/dash/bejne/pWmW1deJmai-lM4.htmlsi=Ney0qpwJGwHzxafH

Superb buddy....

That's what I was looking for💜...thnku sir

@animatedbiologywitharpan

Жыл бұрын

Please share with your friends

Best explanation ever ❤

@animatedbiologywitharpan

Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

Thank you bro Atleast I understand Elisa abit without doing the practical

Thank you sir . Nicely explained 🙏

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Appreciate you really you made it very easy to understand

@animatedbiologywitharpan

2 жыл бұрын

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on Instagram as @animatedbiologywitharpan. Install the app to follow my photos and videos. instagram.com/invites/contact/?i=1p41h314q3fv8&

Hi, Thank you for this great lesson. Can you give me help? I'm doing my presentation about HIV-1, and I don't know to detect p24 antigens, we would use Sandwich indirect or direct Elisa?

@animatedbiologywitharpan

2 жыл бұрын

Sandwitch Elisa would be most appropriate, othet wise direct Elisa may be used

@nhatanhnguyen2084

2 жыл бұрын

@@animatedbiologywitharpan Very helpful, thank you sir.

Thank you, you made it sooo simple

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Really good work and helped me a lot..

@animatedbiologywitharpan

3 жыл бұрын

kzread.info/dron/4IpyopsGWSjaPACNTZLuqg.html please subscribe to my other channel

Well explained sir, thank u.

@animatedbiologywitharpan

Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like Rs50/100 means a lot for us.

Thank you, such a nice explanation 👍👏

@animatedbiologywitharpan

Жыл бұрын

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

superb buddy

@animatedbiologywitharpan

28 күн бұрын

Share with friends

thank you sir, i got a microbiology test and i totally forgot to study the elisa part, i'm really lucky to find this video XD

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

Appreciated

@animatedbiologywitharpan

29 күн бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

Love your videos

@animatedbiologywitharpan

Жыл бұрын

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

thanku so much buddy ♥️♥️♥️🙏🙏🙏🙏

@animatedbiologywitharpan

2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience .

Awesome explanation sir 🥰🥰🥰

@animatedbiologywitharpan

Жыл бұрын

kzread.info/dash/bejne/aop90qWwhJysiNo.html don't forget to checkout competeive ELISA

@animatedbiologywitharpan

Жыл бұрын

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very informative! thanks

@animatedbiologywitharpan

Жыл бұрын

Please share my channel link with your friends and help me to reach big audiance

Well explained 👍🙏

@animatedbiologywitharpan

3 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@monikasharma6426

3 жыл бұрын

@@animatedbiologywitharpan sure

@animatedbiologywitharpan

3 жыл бұрын

Stay tuned and don’t forget to check out my other playlists….

well explained❤️

@animatedbiologywitharpan

Жыл бұрын

You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

Can plasma be also used as sample for the indirect ELISA

Thank u so much this video helps me alot.

@animatedbiologywitharpan

5 жыл бұрын

Great.... now share among your friends and help them as well

@areebayousaf2389

5 жыл бұрын

@@animatedbiologywitharpan haha we friends never share such a type of info.. because of competition uknow...

@animatedbiologywitharpan

5 жыл бұрын

This is not the way you compete it’s very wrong.... help other and be stronger

@areebayousaf2389

5 жыл бұрын

@@animatedbiologywitharpan yes u r alright...

@areebayousaf2389

5 жыл бұрын

@@animatedbiologywitharpan i m just kidding😄

Thank you

@animatedbiologywitharpan

3 жыл бұрын

Please share among friends and help me to reach big audience

Sir well plates is used in so many experiment are reusable or it requires new plate for ech experiment?

@animatedbiologywitharpan

Жыл бұрын

The ELISA 96-well microplates allow performing the common laboratory testing procedure that can be carried out on multiple samples at once.

Thank you l have lab to me this helps me very much

@animatedbiologywitharpan

2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience .

@animatedbiologywitharpan

2 жыл бұрын

Really glad to hear that my contents helped you

Thank you so much!

@animatedbiologywitharpan

Ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@AjoyPaulAdar599

Ай бұрын

@@animatedbiologywitharpan Sure.

Tôi thích sản phẩm này

@animatedbiologywitharpan

Жыл бұрын

I couldn't understand your point

Nice 👍

@animatedbiologywitharpan

Жыл бұрын

Please share my channel link with friends

hi thank you for lesson but I just want to say, I listened 2 times but I found this lecture a little complicated to understand antigen -antikor meanings.. 😔

@animatedbiologywitharpan

2 жыл бұрын

Thanks for your honest opinion....in future videos I would try to simply the material even further

@esrakayikci934

2 жыл бұрын

@@animatedbiologywitharpan thank you for answering me.

Please make a video on PRADA technique

@animatedbiologywitharpan

2 жыл бұрын

Thanks for your suggestion, I will try to make a video on that

Thak you🌷 but what about direct ELISA ? It's used to detect AB or Ag?

@baepahmugo7475

3 ай бұрын

Antigen

Plzzzzzzz send the more videos

I like it 👌👍👍👍

@animatedbiologywitharpan

2 жыл бұрын

Really glad to know it was useful. Please follow my instagrap page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

To detect the presence of hormone we use sandwich elisa but can we use competitive elisa for that?

@animatedbiologywitharpan

Жыл бұрын

Not really recommended

@hudanoor6009

Жыл бұрын

@@animatedbiologywitharpan yes sir but my examiner ask me why?

Hello what is the substrate used after secondary enzyme linked monoclonal body.?

@animatedbiologywitharpan

2 ай бұрын

Some commonly used substrates used in ELISA applications are hydrogen peroxide, luminol, and P-Nitrophenyl-phosphate (pNPP substrate

Savior✨ ✨✨ exam h kal😂😂

@animatedbiologywitharpan

Жыл бұрын

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

Which test is best for hiv diagnosis?? Among all hiv tests ...

@animatedbiologywitharpan

2 жыл бұрын

ElISA is the best way

@Create552

2 жыл бұрын

@@animatedbiologywitharpan elisa test processing time

its ok i loved it

@animatedbiologywitharpan

11 күн бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

Sir ELISA test or EIA test boths are same test

Hi sir I am form karnataka,25 years old i have one doubt. While performing the hiv test with test kit i toughed the area where sample is collected with pricked finger and give the blood. At the time i toughed sampale collection area accidently. I don't use pippet(dropper) I heared live antigen of hiv in the test kit. Now I worried about this. Can Hiv is spread by test kit in such method? I am at risk of getting hiv. Please sir answer this🙏.

@Smileeeyyyy

11 ай бұрын

HIV can only occur if there is a transfusion of a positive blood with negative one. If that happened in your case then get it checked out.

I have one doubt.. In elisa test y we will prefer only serum.. If plasma will possible for test.. in case plasma will we prefer what reaction will happen.. Can you explain please

@animatedbiologywitharpan

2 жыл бұрын

In order to prevent blood clotting during the preparation of plasma anticoagulants commonly are used (like EDTA or heparin). In most cases, anticoagulants affect the interaction of antibodies with antigens, so most test systems designed to work with serum, but not with plasm

thank u g

@animatedbiologywitharpan

2 жыл бұрын

Please share my channel link with your friends and help me to reach big audience

@HarmandeepKaur-

2 жыл бұрын

@@animatedbiologywitharpan okg

whats the difference between direct elisa

@animatedbiologywitharpan

6 ай бұрын

Direct ELISA directly uses labeled antibodies for antigen detection, while indirect ELISA employs unlabeled primary antibodies and labeled secondary antibodies, amplifying the signal for increased sensitivity. Direct ELISA is quicker but less sensitive, while indirect ELISA offers enhanced sensitivity at the cost of additional steps.

Thanku

@animatedbiologywitharpan

10 ай бұрын

Please share my channel link with your friends and help me to reach big audiance

Sir my test valu is 0.08, why this value not be zero, is there any virus exist in my body , pls

What is the substrate in this case of ELISA? Is it the patient's serum?

@animatedbiologywitharpan

10 ай бұрын

ELISA is done on the patient's sample which has the antigen or antibody. If you are asking about the substrate for color reaction then its different

@juniorlucky6195

10 ай бұрын

Yes that's what I mean. What is the substrate for color reaction?

Sir, thank you but competitive Elisa is not explained well

@animatedbiologywitharpan

2 жыл бұрын

Ok I will make a better video on competitive elisa

@toobaaaapi

11 ай бұрын

I agree, I was so excited to have found the perfect video until we reached the competitive ELISA part, still thanks alot sir, it was very well organized and broken down into easy bits of information.

Good

@animatedbiologywitharpan

Ай бұрын

please share my channel link with your friends

I would like to copy it And make a video inn Shah Allah So please must watch

@animatedbiologywitharpan

Жыл бұрын

Whaaaaat ? Why ?

Sir , can any of these types of eliza detect hiv

@animatedbiologywitharpan

3 ай бұрын

Yes

If you use wrong buffer or simple water than also kit will show accurate results

@animatedbiologywitharpan

Жыл бұрын

I am unsure about this

the washing process isn't needed?

@animatedbiologywitharpan

2 жыл бұрын

I agree to your point. Acute this video was an old video , the wash step and the necessity of it should have been highlighted.

Sir, cmia test also elisa

@ashokmalimali4518

Жыл бұрын

Reply sir

Where is animation of Direct Elisa

What is is well?

@animatedbiologywitharpan

17 күн бұрын

Its done in 96 well microtitter plate.

Can you add translation Arabic please ❤️

Well done 👍 thanks

@animatedbiologywitharpan

8 ай бұрын

Please share my channel link with your friends and help me to reach big audiance

I don’t like how HIV is confused with AIDS … or refered to as aids in this video

@beatsbybrando8826

6 ай бұрын

It’s [HIV] not at all being confused with AIDS, HIV is essentially a pre-cursor to AIDS, AIDS is the final stages when the HIV infection becomes most prominent; there are associated decreases to the CD4+ cells, greatly reducing one’s immunity, allowing them to be susceptible to opportunistic infections that the normal person wouldn’t be. Thats how it was discovered; young people having cancers that weren’t typically seen in their age demographic, I.e more of those in their 80s… LONG STORY SHORT, AIDS is HIV, HIV is not CATEGORIZED as AIDS until there is final progression characterized by low CD4+ (T-cell) counts, typically around 200/uL (vs ~1000/uL) , so it’s again the final stage of the infection from the virus, therefore the same thing. It’s a 5-minute summary video, in which he did a great job. 🎉😊

@valliramanan1329

2 ай бұрын

Illiterate 🥱

Why you can't explain direct and Mac Elisa

you should practice it first so the explanation is fluent.

@animatedbiologywitharpan

Жыл бұрын

Yes my friend, thanks for your suggestion. I will incorporate

Why only serum is used

@animatedbiologywitharpan

9 ай бұрын

Serum, the clear liquid portion of blood after clotting, is typically used for diagnostic purposes instead of whole blood because it lacks clotting factors, which could interfere with certain tests. Serum provides a clearer sample for accurate analysis of various biomarkers, such as proteins, enzymes, and antibodies, aiding in diagnostics.

@animatedbiologywitharpan

9 ай бұрын

Serum, the clear liquid portion of blood after clotting, is typically used for diagnostic purposes instead of whole blood because it lacks clotting factors, which could interfere with certain tests. Serum provides a clearer sample for accurate analysis of various biomarkers, such as proteins, enzymes, and antibodies, aiding in diagnostics.

Good, but don't explain the direct method

@animatedbiologywitharpan

Жыл бұрын

Thanks for your detailed feedback

Itna hakla q rha hai bhai

@RockBison-qb3of

2 жыл бұрын

Ek kam karo tum na kudh ka channel khol ke aur khud ke video upload kardo Ek toh is bande ne video me itne efforts Dale hai banane ke liye upar se tumko har cheez perfect chahiye free me Ajkal logo me humanity and understanding kam hoti ja rahi hai

Not okay

@animatedbiologywitharpan

Жыл бұрын

Thanks for your honest feedback

@pharma_b

Жыл бұрын

@@animatedbiologywitharpan You need to give more content on this and you need to explain !! Time doesn't matter,just give a brief Explaination and it will be good 👍

Sir Hindi me bol lo, q English me tuttr puttr kar rahay ho

@animatedbiologywitharpan

11 ай бұрын

My hindi is bad.

Pls improve your narration there are many mistakes

@animatedbiologywitharpan

28 күн бұрын

it was an old video, we will have new videos soon

Sir Please Hindi

@animatedbiologywitharpan

Жыл бұрын

youtube.com/@letscrackbioexams94

Class is good but try to script the content .just blabbering and pausing.

@animatedbiologywitharpan

7 ай бұрын

It was a very old video

@animatedbiologywitharpan

7 ай бұрын

Watch the new one kzread.info/dash/bejne/aop90qWwhJysiNo.htmlsi=QdFH3cvQ6fQqwUb2

dude u stammer a lot

@animatedbiologywitharpan

Жыл бұрын

sorry about that, I am not a native English speaker. This video is very old as well.

@Smileeeyyyy

11 ай бұрын

@@animatedbiologywitharpan it's okay. The stammering wasn't a problem at all. Understood everything👌

worse explanation than my book