Y2H is so much easier to comprehend now. Thank you so much for covering this topic!

Thank you so much for your clear and unambiguous explanations.

This is the best Y2H video I've come across that actually makes sense!

Easy to understand explanation. Thanks Henrik's lab!

Great intro into Y2H, clear and concise! Thanks!

Clear, concise, helpful! Thanks!!

Brief but clear enough to understand!

This is the best Y2H video on youtube - so easy now to understand, thanks a lot!

This is one of the best videos on Y2H. Thank you for making this.

So clearly explained! Thank you!!

You are a life saver. Thank you

Thank you very much Henrik! You are doing a wonderful job with your videos.

@henrikslab

2 жыл бұрын

Thanks!

This was so helpful as I am doing a project right now. Thank you!!

Helped in my understanding thank you

This video is a national treasure.

Awesome, thank you for the clear explanation

Best explanation ever!

Very clear explaination! Big thanks

Thank you. Wonderful explanation.

Very well explained!!!thank you

thank you so much, so clear and concise!

Very nicely explained.

clear very much

danke! endlich mal ein verständliches video :D

Very helpful, friend. Thank you muchly!

Thank you so much.

It’s very helpful, thank you so much.

Thank you it's really clear !

Thanks so much for this helpful video!

This is a great explanation, thank you so much!

Thank you so much, ths video helps me a lot!

very good explanation, thanks

Thank you so much, it helped me so much!

Just amazing👍👍👍👍

Vielen Dank, das war richtig gut erklärt!!

Great video thanks!

Excellent explanation ❤

Thank you

Thanks alot, it was very useful

Thank you so much!

amazing

Very clear thank you so much

thank you very much bro

Well explained thanks ❤❤❤

Easy to understand good job

Thank you!!!

Richtig gutes Video, vielen Dank!! 🙏🏼

@henrikslab

3 жыл бұрын

Freut mich wenns geholfen hat!

clear explanation

Good explanation

Thanks a lot

Nice presentation, thank you, make more videos on rdt

finally i got it . thank uuuu . Allah bless you ❤

Hey thanks a lot for this brilliant video but I have a doubt here.... we take 2 components of the transcription factor AD and DBD and bind the 2 proteins one to each.......how can we say the function of the 2 components does not get altered when one of the proteins is attached to it? Pl help me understand

RNA Polymerase binds to the promoter and transcription factors bind upstream of the promoter.

GJ!

U r great

Does the bacterial adenylate cyclase two-hybrid (BACTH) system also utilise this concept?

Thnx a lot sir...it helped me a lot in undrstanding this concpt💖💖💖

@henrikslab

2 жыл бұрын

Perfect!

Nicely explained. Thanks a lot! Is there a particular transcription factor used for this? Or a criterion for choosing the transcription factor?

@henrikslab

4 жыл бұрын

Good question! So the main criteria is that you have to use a strong transcrption factor which initiates transcription of your reporter gene. In that case here they used Gal4p transcription factor: link.springer.com/protocol/10.1385/1-59259-762-9:247

wow that's a very elegant system. Does it mean then that the proteins need to be taken up into the nucleus in order to be tested, is there limitations in terms of size and charge?

love u

Every other source i found doesn't mention anything about Gal4AD recruiting the polymerase, they just mention that transcription happens. Do you happen to have any sources that show that GAL4AD does indeed recruit the polymerase?

Hi nice video! Hab eine Frage Also dient dieses System lediglich für die Erkenntnis ob Protein a und Protein b interagieren?

@henrikslab

4 жыл бұрын

Ist im Normalfall wirklich ein Verfahren um Interaktionen von zwei Proteinen zu checken, ja!

danke Bruder ich küss dein Auge fürs video

OK so i have 2 questions i hope im not to late 1. how can you bind a protein to DBD or AD 2. what are the usses of this also i very much like your channel

@henrikslab

4 жыл бұрын

Matevz Kosutnik thanks for your question 1. you synthesize a DNA fragment where both sequences are connected and encode a protein with is ´chimericˋ meaning a novel protein out of two individual proteins which are linked via peptide bonds. 2. you can test whether protein A is interacting with protein B (if so, you generate a reporter gene expression)

i love you

@henrikslab

Жыл бұрын

You can discuss this with my girlfriend :D

Ooooooossaaaammm

Thank you so much! I liked your video and it was at 666 likes, so sorry if i ruined it!

@henrikslab

2 жыл бұрын

You did not! The goal is definitely a 1000 likes :D

Thank you

Thank you