Thin Layer Chromatography (TLC)

We know how to perform extraction, which can separate compounds on the basis of differing solubilities. But what if they have the same solubility? Well there is another technique that utilizes differences in polarity, and it's called chromatography. There are many varieties of chromatography, but the best way to introduce the concept will be to look at thin layer chromatography, or TLC for short. Check it out!
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Пікірлер: 140

  • @skxjxhxh
    @skxjxhxh4 жыл бұрын

    You just summarized our 3-hour lesson into 9.5 minutes

  • @mariadelcarmenarteagasanch8822

    @mariadelcarmenarteagasanch8822

    4 жыл бұрын

    exactly, He is the best

  • @emteemte3985

    @emteemte3985

    Жыл бұрын

    He is my hero chemist. I love orga.che. lab because of him

  • @masskiller9206
    @masskiller92063 жыл бұрын

    7:52 "It’s usually difficult to predict what will work best, so just try something and see what happens. " professor dave motivates

  • @drewkrahn
    @drewkrahn2 жыл бұрын

    So much easier to understand than our lab manuals. Thank you!

  • @ramil8029
    @ramil80295 жыл бұрын

    Getting ready for the MCAT exam. Your videos are THE BEST on youtube for all of ochem and gen chem material! Thank you so much!

  • @kevinnguyen1133

    @kevinnguyen1133

    4 жыл бұрын

    Same here!

  • @minaabdulaal5549

    @minaabdulaal5549

    2 жыл бұрын

    Hope you got into med school :)

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    @mr.umbrella3979

    8 ай бұрын

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  • @Alex-gb4vn
    @Alex-gb4vn3 жыл бұрын

    DAVE. Keep up the good work. Thank you so much for your contributions. You really explain things in a manner that is so straightforward and easy to follow. I appreciate you so much.

  • @nikkaagbayani8905
    @nikkaagbayani89053 жыл бұрын

    Mahal na po kita Professor Dave. Ikaw po ang pinakadakilang guro na nakakapagpaintindi saakin ng mga bagay na mahirap intindihin.

  • @meganevans8006
    @meganevans80063 жыл бұрын

    I don't know why o-chem lab manuals have to be mystery novels. Thank you for your videos!

  • @andym.s.5231

    @andym.s.5231

    2 жыл бұрын

    Mystery novels 😭😂😭

  • @patiencechepkorir5596

    @patiencechepkorir5596

    2 жыл бұрын

    I Know right 😂😂

  • @iuliiamairansaeva9169
    @iuliiamairansaeva91692 жыл бұрын

    I'm happy you've decided to do youtube... Some of us watching you right now will become great chemists in the future, and you'll be one of the main reasons we'll manage

  • @lalaland774
    @lalaland7744 жыл бұрын

    Since we’re on quarantine my Organic Chem lab professor is using your videos and basing the work he gives around your videos

  • @danielmeaders4005

    @danielmeaders4005

    4 жыл бұрын

    This honestly seems like an issue of legality and compensation. The situation is the same at my university (however not for this video), I don't think your school is writing checks to professor Dave... but they are certainly getting their monies.

  • @XTheSpartanX7

    @XTheSpartanX7

    4 жыл бұрын

    i wish mine did that

  • @jameshall1300

    @jameshall1300

    Жыл бұрын

    @@danielmeaders4005 it's probably kind of new legal ground to be honest. Before quarantine happened and e-learning became almost the de facto standard in most places, using internet resources so heavily in class was probably very limited. I'm not sure ad revenue really would be fair compensation for that type of usage.

  • @sarahholland5980
    @sarahholland59803 жыл бұрын

    thank you! prepping for lab quizzes using your videos bc they are so much easier to understand than our manuals

  • @senku7996
    @senku79964 ай бұрын

    I'm an msc student and you helped me pass my enterance exam and now helping with my final exams in grad school, you're a life saver! :')

  • @ginamarie7603
    @ginamarie76033 жыл бұрын

    great for visual learners, finally a video that makes it easy to understand. thanks.

  • @R_samurai779
    @R_samurai7793 жыл бұрын

    Thanks a lot man! Our schools told nothing about how this is typically used as an analytic technique

  • @BIAKANOORWorld
    @BIAKANOORWorld5 жыл бұрын

    always satisfied with your explanation prof., a huge fan of you from India

  • @gabyyoga8385
    @gabyyoga83853 жыл бұрын

    Incredibly easy to understand thank you so much!

  • @PG-qn8od
    @PG-qn8od5 жыл бұрын

    Jesus, man, your oc videos are helping me sooo much right now, thank you a lot! ❤️

  • @elisophiaaa
    @elisophiaaa3 жыл бұрын

    well and a simple-explained tutorial. tnx, prof.!

  • @bhawanagahlot9540
    @bhawanagahlot95404 жыл бұрын

    Explaination which u gave is amazing.. u have professional teaching skill...

  • @pallobbarai4152
    @pallobbarai41523 ай бұрын

    Thank you, your lecture is amazing, easy to understand, animation is excellent.

  • @ayasamer7731
    @ayasamer77313 жыл бұрын

    At the morning of my exam .. soo helpful a huge thanks to u ❤

  • @sarahmitchell8344
    @sarahmitchell83443 жыл бұрын

    This has changed my life

  • @ValentinaLopez-bv6ro
    @ValentinaLopez-bv6ro3 жыл бұрын

    Perfect explanation. Thank you

  • @chickenlover4ever
    @chickenlover4ever2 жыл бұрын

    Amazing video, thank you so much!

  • @emteemte3985
    @emteemte3985 Жыл бұрын

    U r unique .i take lab abt tlc anc CC and you make it easy and clear thank you. I wish you good health and long life

  • @mr_mars_vr
    @mr_mars_vr2 жыл бұрын

    Thank you for this.

  • @lyricmelody4650
    @lyricmelody46503 жыл бұрын

    This is so helpful! Thank you :)

  • @veronicaaguiniga8496
    @veronicaaguiniga84962 ай бұрын

    Being a visual learner instead of trying to understand material that needs to be read, I feel that this video on TLC should be shared with students that would like a better insight/understanding on this topic. From watching this video I actually learned that the TLC plate itself has hydroxyl groups that is dipole-dipole, allowing it to interact with other polar substances.

  • @vintilovwhitefox5365
    @vintilovwhitefox536511 ай бұрын

    Thank you dude, it helped me a lot

  • @cesca3994
    @cesca39944 жыл бұрын

    well explained! Thank uuuuuuu.

  • @vvideo7162
    @vvideo7162 Жыл бұрын

    Wow sir it's an amazing lecture I have ever seen thanku so much sir ,for clear my doubts 🥰🥰🥰🥰🥰

  • @mahmoudothman7910
    @mahmoudothman79105 жыл бұрын

    Nice new hair-cut Dave 😊

  • @siddheshdeshpande3034

    @siddheshdeshpande3034

    3 жыл бұрын

    Avadli ka tula

  • @Jdoug305

    @Jdoug305

    2 жыл бұрын

    @@siddheshdeshpande3034 yes I like

  • @siddheshdeshpande3034

    @siddheshdeshpande3034

    2 жыл бұрын

    @@Jdoug305 mr cheeks from Maharashtra

  • @Jdoug305

    @Jdoug305

    2 жыл бұрын

    @@siddheshdeshpande3034 aklakam brother 😁

  • @mawadaabdalaal9916
    @mawadaabdalaal9916 Жыл бұрын

    شكراً جداً بسيط و مختصر ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

  • @luftatmer
    @luftatmer4 жыл бұрын

    man that was a great video!

  • @moonlillac
    @moonlillac Жыл бұрын

    thankyou, it help me so much.

  • @mohinikulkarni4129
    @mohinikulkarni41292 жыл бұрын

    I loved watching this TLC 👍

  • @chemistryforsociety3448
    @chemistryforsociety34483 ай бұрын

    Excellent...... thanks a lot

  • @YouMockMe
    @YouMockMe4 жыл бұрын

    I came back to this a few times haha... great vid

  • @anounymous7988
    @anounymous79882 жыл бұрын

    You're amazing bro god bless you

  • @sweetoldsoul4454
    @sweetoldsoul44542 жыл бұрын

    Thank you 🙏

  • @mariamgad284
    @mariamgad2845 жыл бұрын

    very excellent 💙💙

  • @sohampowdwal8180
    @sohampowdwal8180 Жыл бұрын

    in professor dave we trust

  • @mustakim2144
    @mustakim21443 жыл бұрын

    Thank you!

  • @carolynenglish3068
    @carolynenglish30683 жыл бұрын

    Brilliant!

  • @ihechisomakagha2568
    @ihechisomakagha25688 ай бұрын

    Just Thank you ❤

  • @ParedonesAmarillosNO
    @ParedonesAmarillosNO4 жыл бұрын

    What solvent would you use to prepare the samples for cannabidiols. Thank you for your class! Love learning.

  • @asmaaaboalmagd792
    @asmaaaboalmagd7922 ай бұрын

    Thanks professor

  • @aang7505
    @aang75054 жыл бұрын

    thank you so much! I just wanted to ask, do you benefit more from patreon or from KZread channel memberships? I really appreciate your help, this explanation was so clear

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    4 жыл бұрын

    Patreon takes a smaller cut so that is much better, thanks so much for your support!

  • @chemistry7554
    @chemistry75544 жыл бұрын

    Good personality with hair cut,by the way vedio is help full............

  • @user-kr4ns9vk7j
    @user-kr4ns9vk7j2 жыл бұрын

    Hello Doctor I am now working on an experiment, which is to prepare Schiff bases and run by escalation with the condenser for no less than 6 hours, and I want to follow the end of the interaction with the technique TLC (The solvent used for the reaction is ethanol The question is what polar and nonpolar solvents can be used in chromatography and what ratio shows the endpoint of the reaction )

  • @pintukumargupta8415
    @pintukumargupta841511 ай бұрын

    Nice easy to understand thaks

  • @LOADEDUPTV
    @LOADEDUPTV5 жыл бұрын

    Hello Dave, please talk about Keto Enol Tautomerism

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    5 жыл бұрын

    check my tutorial on michael addition

  • @Ambient_Scenes
    @Ambient_Scenes3 жыл бұрын

    Thank you for the video! One question: If you have no clue what your sample is made of, how do you know whether to use UV light, or a stain to visualize the dots? Also how do you choose the right dye? Also, what do you do if some components of the mixture only get stained by on stain, while other components only get stained by another stain? Is there a huuuuge amount of different stains the organic chemist has to experiment with or are there just a few that will cover pretty much most chemicals?

  • @DapaChrons
    @DapaChrons5 жыл бұрын

    New haircut! looking good either way

  • @tarahclue6737
    @tarahclue67372 жыл бұрын

    Thank you

  • @specialparadise
    @specialparadise5 жыл бұрын

    Wow what a haircut Professor! You look good either way lucky you.

  • @pharmacist9193
    @pharmacist91933 ай бұрын

    Thank u❤❤❤

  • @yasigerman731
    @yasigerman731 Жыл бұрын

    thank you thank you thank you

  • @kikimiki177
    @kikimiki177 Жыл бұрын

    is the polarity of the samples the only thing that impacts how far each of them will travel, or does their relative mass also have an impact?

  • @mmhh3553
    @mmhh355319 күн бұрын

    May Allah bless you and guid you to the all right thing here and and hereafter,,, all the best

  • @ARBB1
    @ARBB15 жыл бұрын

    *Who are you, and what did you do with Jesus?*

  • @-Noushin

    @-Noushin

    3 жыл бұрын

    🙄👉😐👉😑👉😀👉😁👉😂👉🤣

  • @alphagonist4748

    @alphagonist4748

    3 жыл бұрын

    👍🤣😂🤣👍🤣👍🤣💪👎🍌

  • @muhammadumarsarfraz4705

    @muhammadumarsarfraz4705

    7 ай бұрын

    What???

  • @c.u.v2190

    @c.u.v2190

    5 ай бұрын

    Pinned him

  • @jjmeducationcorner1358

    @jjmeducationcorner1358

    3 ай бұрын

    Jesus is a real savior of the world

  • @HeyImLucious
    @HeyImLucious5 жыл бұрын

    If only this video came out a few years ago... I wouldn't have looked like such a fool in my research lab haha

  • @vibscreationvideos4884
    @vibscreationvideos48843 жыл бұрын

    1 ch number bhava ty very much😇🤗

  • @roseycheekies51
    @roseycheekies512 жыл бұрын

    i love you dave

  • @nirmanivimarshana9723
    @nirmanivimarshana97234 жыл бұрын

    nice

  • @sagagayathri9570
    @sagagayathri95705 жыл бұрын

    I had a doubt.... how to know what component has been seperated...and also what is the use of rf value

  • @Thaumius
    @Thaumius5 жыл бұрын

    Will you be doing HPLC/GC tutorials?

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    5 жыл бұрын

    i hadn't planned on it, but i'll put it on the list! it is a big one.

  • @natehcsingh8634
    @natehcsingh86343 жыл бұрын

    Came in jee mains 2021 february attempt . Thanks Jesus↗↗

  • @FullNanochem
    @FullNanochem Жыл бұрын

    Спасибо!

  • @137bob3d
    @137bob3d4 жыл бұрын

    superb presentation. and good of you to cite how TLC is a stepping stone to column chroma' ... and to work out the solvent(s) x:y ratios w/ TLC for upper half separation. eager to see the col. chr. vid's on any time-correlation in top to bottom flows vs TLC. and how you go about collecting the eluent with the separated product and nothing else. also will be listening for info on the details of how one chooses a silica mesh size. and possible re-usability of silica for those of us who know how costly that stuff is. and lastly how to choose column size vs number of grams of material to be separated.

  • @superhessu3
    @superhessu32 жыл бұрын

    4:56 just marking for my upcoming university course

  • @rassimsimou1594
    @rassimsimou1594 Жыл бұрын

    Good

  • @Leen-ih8ql
    @Leen-ih8ql3 жыл бұрын

    what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

  • @chorgulio
    @chorgulio4 жыл бұрын

    So what is the typical solvent used for TLC? would that depend of the sample/compound to be analyzed?

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    4 жыл бұрын

    in the lab i would typically use a mixture of hexanes and ethyl acetate. it's kind of personal preference to an extent.

  • @karenopt
    @karenopt4 жыл бұрын

    If the solvent is polar, then the polar molecules will have a smaller Rf and non polar molecules would have a larger Rf? Or is it the other way around?

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    4 жыл бұрын

    yes you have it right!

  • @esmeralda804
    @esmeralda8049 ай бұрын

    ME ENCANTAAAAA❣

  • @benzjoshwade3462
    @benzjoshwade34623 жыл бұрын

    Hello. This might be a dumb question but why does Silica (SiO2) have hydroxy groups?

  • @elizaahmed2066
    @elizaahmed20663 ай бұрын

    Sir my compounds are non polar compounds. so which solvent do I use for dissove the compound and what solvents do I use in the mobile phase? sir can you tell me a little bit? I am facing a lot of problems in tlc checking

  • @tanzimnishad3892
    @tanzimnishad3892 Жыл бұрын

    Sir How can I separate a mixture with 37 compounds in it using column chromatography

  • @saiteja6742
    @saiteja67424 жыл бұрын

    Sir why do we use organic solvent(toulene) as mobile phase in thin layer chromatography ?? Pls reply for this sir

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    4 жыл бұрын

    what else would we use?

  • @prateekkulkarni2639
    @prateekkulkarni26392 ай бұрын

    A day before jee mains 2024

  • @marniejones331

    @marniejones331

    26 күн бұрын

    Kya mtlb Indian log smart rahte hai

  • @kennyyong7694
    @kennyyong76943 жыл бұрын

    Excuse me, do you know how to prepare silica gel to absorb moisture or silica gel to act as the stationary phase in thin layer chromatography. I need to make my own TLC plate

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    3 жыл бұрын

    Hmm no I've never made plates before. But check the tutorial that follows this one on column chromatography, it may help a little.

  • @ayaalhashimi3100
    @ayaalhashimi31002 жыл бұрын

    Please what mean tlc its same column ?

  • @akimhaq1616
    @akimhaq16164 жыл бұрын

    👍🏻

  • @Ambient_Scenes
    @Ambient_Scenes3 жыл бұрын

    Why do you need a capillary tube to place the solution mixture on the plate? Can't you just use a pipette or dropper or something?

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    3 жыл бұрын

    the capillary tube is much better for applying the solution because you press it against the plate and it is adsorbed, a dropper would be terribly imprecise

  • @Ambient_Scenes

    @Ambient_Scenes

    3 жыл бұрын

    @@ProfessorDaveExplains Thanks for clarifying.

  • @fatimasaid648
    @fatimasaid6483 жыл бұрын

    I’m confused to why the dots are not being places with marker but are placed with a capillary tube ??

  • @ProfessorDaveExplains

    @ProfessorDaveExplains

    3 жыл бұрын

    the capillary tube loads the sample onto the plate

  • @ammarahmed3970
    @ammarahmed39703 жыл бұрын

    you are so handsome and ur method is also smart and interesting.

  • @user-mh2zj6sj6q
    @user-mh2zj6sj6q3 ай бұрын

    where's Dave's hair?

  • @Sandeepnishad90
    @Sandeepnishad905 жыл бұрын

    Looking handsome

  • @Textbook000
    @Textbook0005 жыл бұрын

    Like like like this

  • @markmessi335
    @markmessi3352 жыл бұрын

    what is the point of the mobile fase? we have to optimize on the mobile fase in a project, but what does it do exactly?

  • @joslynsantana8236
    @joslynsantana82364 жыл бұрын

    🙌🏽💞

  • @miyamotomushashi409
    @miyamotomushashi4094 ай бұрын

    gggreat

  • @MarianMatejdes
    @MarianMatejdes Жыл бұрын

    Lets get some THC :D

  • @hardhard8943
    @hardhard89432 жыл бұрын

    Thank you Arabic

  • @mrmust2780
    @mrmust27803 жыл бұрын

    are you really professor Dave? where is your hair?

  • @S_H428
    @S_H4285 жыл бұрын

    Wow nooo get ur hair BACK PLEASSEEE

  • @Bludgeoned2DEATH2
    @Bludgeoned2DEATH25 жыл бұрын

    TLC is so boring 😩 HPLC is way better 👍🏽