RT - PCR (Real Time PCR) | Quantitative - PCR | qPCR | Basic Principle and Data Analysis
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Real-time PCR (#RT-PCR), or quantitative PCR (#qPCR), is a highly sensitive molecular biology technique used to amplify and simultaneously quantify DNA or RNA in real-time as the amplification occurs. Unlike traditional PCR, which provides an endpoint measurement of amplified DNA after the reaction is completed, real-time PCR allows for continuous monitoring of the amplification process. This real-time monitoring is achieved through the use of fluorescent dyes or probes that emit fluorescence as DNA or RNA is replicated.
Primer Designing - 👉 • Primer Designing PCR |...
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#rtpcr #rtpcrtest #biotechnology #pharmacy #biotech #pcr #science
#BaaYo
Пікірлер: 106
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Teaching is an Art. As student of zoology I only say thank you for such simple way of teaching
@BaaYo
11 ай бұрын
Thank you
Such a quality education!!! Cheers for it and advance HAPPY TEACHERS DAY to u ❤❤
@BaaYo
10 ай бұрын
Thanks a ton
Aap nae janti but aap bht easy krdeti han har chz 😂🎀 Keep on covering my BS syllabus please🌝
Thank you so much mam. You explained this difficult topic in a very simple way..😢❤ Huge respect and love from Pakistan.👏🙌
I am first year mbbs student and couldn't understand this topic not in my college and neither on any other platform. But you really made this easy for me. Thanks.
@BaaYo
10 ай бұрын
Thank you Zaid
You explained the topic so well. Thanks a lot
To access notes and practice questions, please join our Telegram channel t.me/baayo_official
So helpful, many thanks❤
Thank you It was easily understandable for exam point of view
Nice 👍 thankyou mam for besic revision
All concepts cleared
Very helpful..tq so much
Thank u mam! Really helpful Love u mam.....
very well explained Ma'am...
Mam aap bhott acha smjaati hu kaash hmaare college k time pr aapke lecture dekhe hote toh ache number score kr pata
Thanku
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).
Going for a interview tomorrow and spent the whole day here
@BaaYo
11 ай бұрын
All the very best for tomorrow:)
Thank you ma'am for explaining very clearly.
@BaaYo
10 ай бұрын
Thanks for liking
Thank you mam❤
Thank you so much ❤ Your videos help me to strengthen my concepts
@BaaYo
10 ай бұрын
Please share with your friends ❤️
Lots of love from united states
A lot of thanks ma'am
You're my favorite mam ❤️🥰
thanku dii❤
Nice ❤
Thank u so much ma'am
Medam so nice lecture love from ❤ Pakistan
Well explained mam 👌🏻👌🏻 Thank you ma'am 🥰
@BaaYo
11 ай бұрын
Thank u dear
Thnx mam ❤❤
Its really very helpful mam, thank you so much dear mam 😊
@BaaYo
Жыл бұрын
Thank you
Ma,am Aap 4 year,s mai bilkull b change nhi hui... Pehley b Gorgeous thi or ab b...illlaa MashaAllah...😊❤
@BaaYo
11 ай бұрын
Thanks 😊
Superb ma'am
@BaaYo
Жыл бұрын
Thank You :)
👌👌👌
Really Helpful mam
@BaaYo
Жыл бұрын
Thank you
Crystal clear ho gayi 😊
@BaaYo
11 ай бұрын
Thank you dear ❤️
Nyc explanation mam❤❤
@BaaYo
10 ай бұрын
Thanks a lot 😊
Thank you Ma'am.
@BaaYo
11 ай бұрын
Thank you:)
Maam aap bahut hi achi tarah se explain karti hai
@BaaYo
Жыл бұрын
Thank you
Is chapter ko parne ki liye abhi book open kiya hai or apka video ka notification bhi agaya net on kiya to... Nice coincidence 🔥🔥🔥😅😅
@BaaYo
Жыл бұрын
I hope aapko samajh aaya hoga topic..
❤
Mam please make more videos on instrumentation.
👍
Thank you maam😍
@BaaYo
Жыл бұрын
Thanks Kajal 😊
It is really very helpful madam.thank you so much....please create also video simple PCR and sequence seqstudio thermofisher
@BaaYo
11 ай бұрын
Sure.. thanks
Thanks mam
@BaaYo
Жыл бұрын
Keep watching
Thanks mam ,,,, I want your special class for siqwancing , Please help
Mam plzzzz RFLP and ARMS pcr ke primer designing aur practically how can we performed ARMS and RFLP pcr.
@BaaYo
10 ай бұрын
Ok.. noted
@ammarahmehak9986
10 ай бұрын
Thanx mam for your reply in ok.
Kal internship ke report ka viva hein, Thankyou mam 🙂
@BaaYo
10 ай бұрын
how was it? :)
@timothy8048
10 ай бұрын
@@BaaYo just done with viva, i answered all of the questions correctly, let's see how many marks she has given Actually I am preparing for CAT, just mistakenly took admission in biotechnology, but ur videos literally save me everytime a day before exam or viva Even with 0 interest i still score 8.4 sgpa in every sem 🙂 all thanks to u Once again Thankyou from a guy who doesn't like biotech at all 😂
@BaaYo
10 ай бұрын
Hahaha best wishes
why mam is so beautiful❤❤
RT pcr ma hum RNA single strand use nh karte ?
Ekdum Bakwas padhaya ae topic apne ...kitna confuse kr rahai ho PCR and rtPCR me
Mam reverse transcriptase pcr, inverse, nested etc inke upar v ho sake video baniyega
@BaaYo
Жыл бұрын
Sure
Thank you very much ma'am for your support 🙏
@BaaYo
11 ай бұрын
Thank you
Plz make video on immunoflouresence assay
@BaaYo
11 ай бұрын
Sure..
Maam wurtz fitting reaction free radical substitution halogenations kya hota hai maam pura video banaiye n es pr
@BaaYo
Жыл бұрын
Noted
Maam kindly explain primers .... Becaise we have confusion If we have unknown DNA temple how primer will join..
@BaaYo
11 ай бұрын
Ya next video will b about primers..
@ikrampanhwar
11 ай бұрын
Thanku ma'am ❤
Mam what is the detection process of Rt-PCR..?
@BaaYo
10 ай бұрын
See the amplification curve.. if it is there then case is positive
Maam aap keval chemistry hi padhati hai ya aapke pass math ke bhi teachers hai
@BaaYo
11 ай бұрын
Lifescience.. biotechnology no maths
Mam 7:4 min pe aap ne jo bataya wo nhi samjha. 5 terminal aur 3 terminal nhi samjh raha kya hota hai. Aur quancher kya hai.
@BaaYo
Жыл бұрын
Terminal ke bare me mai dobara explain krugi.. for this you need to understand DNA structure
@ps-wj5ib
Жыл бұрын
@@BaaYo ok mam. Humko lab me SOP file nhi dete padhne k liye. Main quantstudi pe rtpcr lagata hoo. Thank you.
Heloo gd evg mam.... Mam please help me i want to qualify my jrf but i Failed from my three attempt..... Please help me mam ki mi kiasey kru or galti kya ho rhi h mhuj se.... Mam miney basic per cammand kiye h but advance tak kisey le jayu....plz guide me mam
@BaaYo
11 ай бұрын
Msg me on telegram
😢kya ct
Not so much helpful.....need more explanation about CT value
@BaaYo
11 ай бұрын
What u want to know tell me.. it is just an introductory video..
Superb mam
@BaaYo
Жыл бұрын
Thanks for watching my videos 👍🏼
Thank you so much ma'am
@BaaYo
Жыл бұрын
Thank you
Thank you mam ❤
@BaaYo
Жыл бұрын
Thank you Dishant