RT - PCR (Real Time PCR) | Quantitative - PCR | qPCR | Basic Principle and Data Analysis

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Real-time PCR (#RT-PCR), or quantitative PCR (#qPCR), is a highly sensitive molecular biology technique used to amplify and simultaneously quantify DNA or RNA in real-time as the amplification occurs. Unlike traditional PCR, which provides an endpoint measurement of amplified DNA after the reaction is completed, real-time PCR allows for continuous monitoring of the amplification process. This real-time monitoring is achieved through the use of fluorescent dyes or probes that emit fluorescence as DNA or RNA is replicated.
Primer Designing - 👉 • Primer Designing PCR |...
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Пікірлер: 106

  • @BaaYo
    @BaaYo19 күн бұрын

    Join our CSIR Life Science December Course with affordable monthly payments: bit.ly/4cDkFTP

  • @jabbarchoudhary8923
    @jabbarchoudhary892311 ай бұрын

    Teaching is an Art. As student of zoology I only say thank you for such simple way of teaching

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank you

  • @tanushri4236
    @tanushri423611 ай бұрын

    Such a quality education!!! Cheers for it and advance HAPPY TEACHERS DAY to u ❤❤

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Thanks a ton

  • @syedakhadeeeja
    @syedakhadeeeja23 күн бұрын

    Aap nae janti but aap bht easy krdeti han har chz 😂🎀 Keep on covering my BS syllabus please🌝

  • @hafeezulhaq9920
    @hafeezulhaq99208 ай бұрын

    Thank you so much mam. You explained this difficult topic in a very simple way..😢❤ Huge respect and love from Pakistan.👏🙌

  • @zaidansari882
    @zaidansari88210 ай бұрын

    I am first year mbbs student and couldn't understand this topic not in my college and neither on any other platform. But you really made this easy for me. Thanks.

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Thank you Zaid

  • @kritikarana5323
    @kritikarana53236 ай бұрын

    You explained the topic so well. Thanks a lot

  • @BaaYo
    @BaaYo11 ай бұрын

    To access notes and practice questions, please join our Telegram channel t.me/baayo_official

  • @Ashmita_31
    @Ashmita_313 ай бұрын

    So helpful, many thanks❤

  • @harsheetagupta163
    @harsheetagupta1633 ай бұрын

    Thank you It was easily understandable for exam point of view

  • @kavimishra7496
    @kavimishra74965 ай бұрын

    Nice 👍 thankyou mam for besic revision

  • @amnasaeed4429
    @amnasaeed44297 ай бұрын

    All concepts cleared

  • @choureyharshi2268
    @choureyharshi226810 ай бұрын

    Very helpful..tq so much

  • @muhammadhasnainofficial2263
    @muhammadhasnainofficial22636 ай бұрын

    Thank u mam! Really helpful Love u mam.....

  • @meghachaudhari2466
    @meghachaudhari24667 ай бұрын

    very well explained Ma'am...

  • @punitkumar6770
    @punitkumar67707 ай бұрын

    Mam aap bhott acha smjaati hu kaash hmaare college k time pr aapke lecture dekhe hote toh ache number score kr pata

  • @Vision-313
    @Vision-313Күн бұрын

    Thanku

  • @arddhendusahoo6590
    @arddhendusahoo65905 ай бұрын

    Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).

  • @diksha_vlogs_N_shorts
    @diksha_vlogs_N_shorts11 ай бұрын

    Going for a interview tomorrow and spent the whole day here

  • @BaaYo

    @BaaYo

    11 ай бұрын

    All the very best for tomorrow:)

  • @mithingaboro8303
    @mithingaboro830310 ай бұрын

    Thank you ma'am for explaining very clearly.

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Thanks for liking

  • @bsbk6065
    @bsbk6065Ай бұрын

    Thank you mam❤

  • @navinasimoes
    @navinasimoes10 ай бұрын

    Thank you so much ❤ Your videos help me to strengthen my concepts

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Please share with your friends ❤️

  • @user-io9le4de5j
    @user-io9le4de5j2 ай бұрын

    Lots of love from united states

  • @manish7836
    @manish783611 ай бұрын

    A lot of thanks ma'am

  • @hamnatariq3653
    @hamnatariq36532 ай бұрын

    You're my favorite mam ❤️🥰

  • @arundhathisg4356
    @arundhathisg43564 ай бұрын

    thanku dii❤

  • @dsk10
    @dsk108 ай бұрын

    Nice ❤

  • @masratrasheed1852
    @masratrasheed18526 ай бұрын

    Thank u so much ma'am

  • @tahsindawar2874
    @tahsindawar28748 ай бұрын

    Medam so nice lecture love from ❤ Pakistan

  • @Lifescience_aspirant
    @Lifescience_aspirant Жыл бұрын

    Well explained mam 👌🏻👌🏻 Thank you ma'am 🥰

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank u dear

  • @tanukumawat797
    @tanukumawat7973 ай бұрын

    Thnx mam ❤❤

  • @aartiverma5608
    @aartiverma5608 Жыл бұрын

    Its really very helpful mam, thank you so much dear mam 😊

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @govind-ow8wf
    @govind-ow8wf Жыл бұрын

    Ma,am Aap 4 year,s mai bilkull b change nhi hui... Pehley b Gorgeous thi or ab b...illlaa MashaAllah...😊❤

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thanks 😊

  • @arunkumarr1279
    @arunkumarr1279 Жыл бұрын

    Superb ma'am

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank You :)

  • @HammadKhan2k
    @HammadKhan2k9 ай бұрын

    👌👌👌

  • @ranjitkumarpanda128
    @ranjitkumarpanda128 Жыл бұрын

    Really Helpful mam

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @kaminisahoo1803
    @kaminisahoo180311 ай бұрын

    Crystal clear ho gayi 😊

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank you dear ❤️

  • @Kaur_2343
    @Kaur_234311 ай бұрын

    Nyc explanation mam❤❤

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Thanks a lot 😊

  • @dr_mbbt
    @dr_mbbt11 ай бұрын

    Thank you Ma'am.

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank you:)

  • @mangaleshwarprasad7936
    @mangaleshwarprasad7936 Жыл бұрын

    Maam aap bahut hi achi tarah se explain karti hai

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @SOURAVMONDAL-fb1cm
    @SOURAVMONDAL-fb1cm Жыл бұрын

    Is chapter ko parne ki liye abhi book open kiya hai or apka video ka notification bhi agaya net on kiya to... Nice coincidence 🔥🔥🔥😅😅

  • @BaaYo

    @BaaYo

    Жыл бұрын

    I hope aapko samajh aaya hoga topic..

  • @reet1825
    @reet1825 Жыл бұрын

  • @uu1238
    @uu12386 ай бұрын

    Mam please make more videos on instrumentation.

  • @muneshkumar7537
    @muneshkumar75372 ай бұрын

    👍

  • @kajalagrawal02
    @kajalagrawal02 Жыл бұрын

    Thank you maam😍

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks Kajal 😊

  • @aliabbass3720
    @aliabbass3720 Жыл бұрын

    It is really very helpful madam.thank you so much....please create also video simple PCR and sequence seqstudio thermofisher

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Sure.. thanks

  • @Shabbir2749
    @Shabbir2749 Жыл бұрын

    Thanks mam

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Keep watching

  • @rekhagautam2905
    @rekhagautam29059 ай бұрын

    Thanks mam ,,,, I want your special class for siqwancing , Please help

  • @ammarahmehak9986
    @ammarahmehak998611 ай бұрын

    Mam plzzzz RFLP and ARMS pcr ke primer designing aur practically how can we performed ARMS and RFLP pcr.

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Ok.. noted

  • @ammarahmehak9986

    @ammarahmehak9986

    10 ай бұрын

    Thanx mam for your reply in ok.

  • @timothy8048
    @timothy804810 ай бұрын

    Kal internship ke report ka viva hein, Thankyou mam 🙂

  • @BaaYo

    @BaaYo

    10 ай бұрын

    how was it? :)

  • @timothy8048

    @timothy8048

    10 ай бұрын

    @@BaaYo just done with viva, i answered all of the questions correctly, let's see how many marks she has given Actually I am preparing for CAT, just mistakenly took admission in biotechnology, but ur videos literally save me everytime a day before exam or viva Even with 0 interest i still score 8.4 sgpa in every sem 🙂 all thanks to u Once again Thankyou from a guy who doesn't like biotech at all 😂

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Hahaha best wishes

  • @TiNTiN-nj7jg
    @TiNTiN-nj7jg2 ай бұрын

    why mam is so beautiful❤❤

  • @ameermarri7385
    @ameermarri73859 ай бұрын

    RT pcr ma hum RNA single strand use nh karte ?

  • @apnagyan7001
    @apnagyan70013 ай бұрын

    Ekdum Bakwas padhaya ae topic apne ...kitna confuse kr rahai ho PCR and rtPCR me

  • @pratitidas
    @pratitidas Жыл бұрын

    Mam reverse transcriptase pcr, inverse, nested etc inke upar v ho sake video baniyega

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Sure

  • @kaminisahoo1803
    @kaminisahoo180311 ай бұрын

    Thank you very much ma'am for your support 🙏

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank you

  • @masoomarazi212
    @masoomarazi21211 ай бұрын

    Plz make video on immunoflouresence assay

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Sure..

  • @mangaleshwarprasad7936
    @mangaleshwarprasad7936 Жыл бұрын

    Maam wurtz fitting reaction free radical substitution halogenations kya hota hai maam pura video banaiye n es pr

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Noted

  • @ikrampanhwar
    @ikrampanhwar11 ай бұрын

    Maam kindly explain primers .... Becaise we have confusion If we have unknown DNA temple how primer will join..

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Ya next video will b about primers..

  • @ikrampanhwar

    @ikrampanhwar

    11 ай бұрын

    Thanku ma'am ❤

  • @shihabsakib9906
    @shihabsakib990611 ай бұрын

    Mam what is the detection process of Rt-PCR..?

  • @BaaYo

    @BaaYo

    10 ай бұрын

    See the amplification curve.. if it is there then case is positive

  • @mangaleshwarprasad7936
    @mangaleshwarprasad7936 Жыл бұрын

    Maam aap keval chemistry hi padhati hai ya aapke pass math ke bhi teachers hai

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Lifescience.. biotechnology no maths

  • @ps-wj5ib
    @ps-wj5ib Жыл бұрын

    Mam 7:4 min pe aap ne jo bataya wo nhi samjha. 5 terminal aur 3 terminal nhi samjh raha kya hota hai. Aur quancher kya hai.

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Terminal ke bare me mai dobara explain krugi.. for this you need to understand DNA structure

  • @ps-wj5ib

    @ps-wj5ib

    Жыл бұрын

    @@BaaYo ok mam. Humko lab me SOP file nhi dete padhne k liye. Main quantstudi pe rtpcr lagata hoo. Thank you.

  • @LIVEBIOS_by_RAK
    @LIVEBIOS_by_RAK Жыл бұрын

    Heloo gd evg mam.... Mam please help me i want to qualify my jrf but i Failed from my three attempt..... Please help me mam ki mi kiasey kru or galti kya ho rhi h mhuj se.... Mam miney basic per cammand kiye h but advance tak kisey le jayu....plz guide me mam

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Msg me on telegram

  • @tusarbehera6359
    @tusarbehera635910 ай бұрын

    😢kya ct

  • @rb11ism
    @rb11ism11 ай бұрын

    Not so much helpful.....need more explanation about CT value

  • @BaaYo

    @BaaYo

    11 ай бұрын

    What u want to know tell me.. it is just an introductory video..

  • @geneticsnandukahate3303
    @geneticsnandukahate3303 Жыл бұрын

    Superb mam

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks for watching my videos 👍🏼

  • @COMICALCREATION
    @COMICALCREATION Жыл бұрын

    Thank you so much ma'am

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @DishantPatkar
    @DishantPatkar Жыл бұрын

    Thank you mam ❤

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you Dishant