CRISPR | CRISPR - CAS9 | Genome Editing Tool

What is CRISPR-Cas9?
CRISPR-Cas9 is a revolutionary gene-editing technology that has garnered significant attention in the field of genetics and molecular biology. It allows scientists to precisely modify the DNA of living organisms, offering a powerful tool to manipulate genes and potentially treat genetic diseases.
CRISPR-Cas9 Mechanism:
The CRISPR-Cas9 system works in several steps:
1) Adaptation: Bacteria encounter foreign DNA (e.g., from viruses) and incorporate fragments of this DNA into their CRISPR region.
2) CRISPR RNA Production: The CRISPR region is transcribed into CRISPR RNA (crRNA), which forms a complex with trans-activating CRISPR RNA (tracrRNA) to guide the Cas9 enzyme.
3) Targeting: The CRISPR-Cas9 complex scans the cell's DNA to find a sequence that matches the guide RNA (crRNA-tracrRNA complex).
4) Editing: Once the target DNA sequence is found, Cas9 cuts the DNA strand. This break can then be repaired through the cell's own repair mechanisms, which may introduce small mutations or allow researchers to insert or remove specific genetic material at the site of the cut.
Applications of CRISPR-Cas9:
CRISPR-Cas9 has numerous applications, including:
1) Basic research to study gene functions and understand biological processes.
Biomedical research for potential treatments of genetic diseases by correcting or modifying faulty genes.
2) Agricultural applications to develop crops with improved characteristics and resistance to diseases.
3) Biotechnology for engineering microorganisms to produce useful compounds or materials.
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#crispr #biotechnology #biology

Пікірлер: 111

  • @BaaYo
    @BaaYo11 күн бұрын

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  • @sagarmeena0210
    @sagarmeena02105 ай бұрын

    thank you for explaining so clearly

  • @BaaYo
    @BaaYo11 ай бұрын

    To access notes and practice questions, please join our Telegram channel t.me/baayo_official

  • @sayedsajid2529
    @sayedsajid25292 ай бұрын

    It was a healthy one with alot of information in such shot duration. Thankfull❤

  • @hafeezulhaq9920
    @hafeezulhaq99207 ай бұрын

    Mam, huge respect for you.. You made our tomorrow easy. I was totally confused about this technique but now 100% clear. Thanks

  • @kajalagrawal02
    @kajalagrawal02 Жыл бұрын

    Apki jitni tarif kare, utni kaam h ma'am, 😍 thank you so much ❤️

  • @BaaYo

    @BaaYo

    Жыл бұрын

  • @Gdsthccg4577
    @Gdsthccg45774 ай бұрын

    I was about to die without this class 😢... Thank you so much 👍

  • @BaaYo

    @BaaYo

    4 ай бұрын

    I hope all well now 😃

  • @smritilekha2935
    @smritilekha2935 Жыл бұрын

    Thank you so much ma'am! It's just to the point explanation! ❤️❤️❤️❤️

  • @BaaYo

    @BaaYo

    Жыл бұрын

    ❤❤

  • @organicanalyticalchemistry1676
    @organicanalyticalchemistry1676 Жыл бұрын

    Thank you 👍

  • @shubhanshisisodiya7622
    @shubhanshisisodiya762211 ай бұрын

    Teacher app jese hoto padhne or smjhne m b mja ata h maam ❤ #Nice teacher 🏆

  • @nilaratnadas5246
    @nilaratnadas52469 ай бұрын

    very nicely explained. thank you mam

  • @ShivamSingh-fk8sg
    @ShivamSingh-fk8sg Жыл бұрын

    Thank you ma'am for such an easy explanation 🙏🙏...

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you Shivam

  • @twinkle1548
    @twinkle154811 ай бұрын

    Thank you sooo much ma'am every video of yours are soo informative and useful thank you soo soo much💐😊👍🙏

  • @BaaYo

    @BaaYo

    11 ай бұрын

    My pleasure 😊

  • @Khushivrm1
    @Khushivrm16 ай бұрын

    Thank uh for this concept vd

  • @chhavirawat5500
    @chhavirawat550011 ай бұрын

    Very easy explanation now I understand each an every point.... thankyou so much ma'am......😊

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thanks dear... please share with your batchmates also...

  • @vandanarana354
    @vandanarana354 Жыл бұрын

    Very well explained 👏

  • @BaaYo

    @BaaYo

    Жыл бұрын

  • @shivamraja9978
    @shivamraja9978Ай бұрын

    Sukriya mam🌿

  • @pinkeshkumar5274
    @pinkeshkumar5274 Жыл бұрын

    Excellent detail explanation

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks

  • @noorulain1640
    @noorulain1640 Жыл бұрын

    First chanel found best for biotechnology

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank u.. please share with ur batchmates also

  • @sundaslaraib5699
    @sundaslaraib56993 ай бұрын

    Finally got it🎉....Thanks mam❤

  • @Simran-th5fq
    @Simran-th5fq4 ай бұрын

    best youtube channel i have ever seen .. thankyou mam

  • @BaaYo

    @BaaYo

    4 ай бұрын

    Thanks a lot 😊

  • @vinaypundir4519
    @vinaypundir4519 Жыл бұрын

    Good 🎉 nice going...keep it up.

  • @wisdomaticconcepts798
    @wisdomaticconcepts7986 ай бұрын

    Excellent

  • @pramodsingh7569
    @pramodsingh75693 ай бұрын

    Thanks

  • @AkhileshSingh-go1rw
    @AkhileshSingh-go1rw5 ай бұрын

    Thank you very much madam for nice delineation

  • @dipalitripathilab
    @dipalitripathilab11 ай бұрын

    Thank you ma'am ❤ 😊

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Keep watching

  • @DrMukaddamSadi
    @DrMukaddamSadi7 ай бұрын

    Awesome

  • @qaiserali4378
    @qaiserali43786 ай бұрын

    thank you mam

  • @dsk10
    @dsk108 ай бұрын

    Nice ❤

  • @tejpratapsingh4684
    @tejpratapsingh4684 Жыл бұрын

    Achha padati hai aap!!

  • @manish7836
    @manish783611 ай бұрын

    Very useful session a lot of thanks ma'am

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thanks for liking

  • @ayushvishwakarma3568
    @ayushvishwakarma3568 Жыл бұрын

    the video we were waiting for a nice explanation mam thank you......

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @ayushvishwakarma3568

    @ayushvishwakarma3568

    Жыл бұрын

    @@BaaYo mam please make video on bioinformatics

  • @hajrabibi8773
    @hajrabibi87739 ай бұрын

    Thank you very much. As you have already said ki disruption krni hai toh DNA ki originality loose ho jayegi magar hum toh chahte ho hain ki Dna mein certain modification laayein toh originality toh wese bhi loose ho hi jati na Please explain it mam Btw your lectures are helping tools & mostly before examinations

  • @Gian_Hein_Ap
    @Gian_Hein_Ap Жыл бұрын

    Everything is temporary but mam's same fashion sense is permanent ❤

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Haha

  • @maheshswami5226
    @maheshswami5226 Жыл бұрын

    First viewer, very very nice explanation, no words left for praise ❤, thank you so much ma'am 💞

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks a lot

  • @nadeemiqbal4742
    @nadeemiqbal4742 Жыл бұрын

    Nice lecture.

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank you

  • @AliAshif8719
    @AliAshif8719 Жыл бұрын

    Well define mam☺️🌹

  • @ujjwalrana3871
    @ujjwalrana3871 Жыл бұрын

    Great explanation mam😊

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thank u

  • @kanchandas3807
    @kanchandas380711 ай бұрын

    Ma'am or friend.where can i find previous years question paper without answer in it from internet of life sciences

  • @user-hu5sp4fv7o
    @user-hu5sp4fv7o4 ай бұрын

    Mdm yi crisper cas9 jb hm gene editing ki li use krti hy to wo kis trh ja ki ak gene ki sat ja ki bind kri ga q k gene ka complentery sequence to already gene mi hota hy .to crisper case ki zary editing krni si hm ligase use kri gy k wo ja k os complementary sequence ko ak dosry si seprat ki bad mi crisper ja ki wah pi bind kri ya koi aur mechnism hy

  • @aswalaswal6321
    @aswalaswal6321 Жыл бұрын

    Very good mam

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks a lot

  • @kartikeyverma6651
    @kartikeyverma665111 ай бұрын

    During cloning, how does the phage DNA integrate with the host cell genome and replicate if the bacterial genome contains a CRISPR locus?

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Cas protein does this part.. integration

  • @ambujpandey7610
    @ambujpandey761011 ай бұрын

    Welcome back ma'am

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Thank you

  • @ambujpandey7610

    @ambujpandey7610

    11 ай бұрын

    @@BaaYo it's my pleasure

  • @RakeshKumar-br4tl
    @RakeshKumar-br4tl Жыл бұрын

    Nice ma'am

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Thanks

  • @rashmisingh717
    @rashmisingh7177 ай бұрын

    Mam please tell about crspr cas type 4

  • @rupeshverma1962
    @rupeshverma196210 ай бұрын

    Very nice Mam.., and please make a video on Diagnostic

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Sure I will

  • @Supriya2627
    @Supriya26279 ай бұрын

    5.08 ke bad suddenly kaha shift hogyi ma'am i mean series wise nhi h lecture

  • @harshitareddyharshitareddy9607
    @harshitareddyharshitareddy9607 Жыл бұрын

    Your teaching is good sister..... Please make more and more vedios on biotechnology for msc students

  • @BaaYo

    @BaaYo

    Жыл бұрын

    thanks

  • @harshitareddyharshitareddy9607

    @harshitareddyharshitareddy9607

    Жыл бұрын

    How are sister

  • @harshitareddyharshitareddy9607

    @harshitareddyharshitareddy9607

    Жыл бұрын

    @@BaaYowhat is your education status sis

  • @Activelearner-rm7by
    @Activelearner-rm7by9 ай бұрын

    Please tell me about more information.

  • @shreyatiwari3054
    @shreyatiwari3054 Жыл бұрын

    Mam itna time bad video upload kiya ...please continue kriye ise please...regular post kriye mam

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Yes

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Sure 👍🏼, Thanks for watching!

  • @Sanakhan-tf5xi
    @Sanakhan-tf5xi Жыл бұрын

    Mam paramesium k morphology and reproduction p video daliye plz..

  • @archanasinghal2192
    @archanasinghal2192 Жыл бұрын

    mam what is your source of information as i am working on topic "crispr in infection control" . It would be helpful if you can share some information related to this. Thankyou

  • @samyakmoon7235

    @samyakmoon7235

    Жыл бұрын

    will you share with me if any articles or paper you have got, cause our topic is similar

  • @ecri
    @ecri8 ай бұрын

    how is gene editing? Can we have white akın, blonde hair, blue eyes without Being reborn? İs this true? İs such a thing really possible? If so, when will it be operational worldwide? İs it okay to use this for health or cosmetic purposes?

  • @sagarnagnekar28
    @sagarnagnekar2811 ай бұрын

    Yekab launch hoga aur cost kitna hoga

  • @sakshitripathi.8003
    @sakshitripathi.800311 ай бұрын

    Hi mam bsc 5th sem ka zoology kab padhayegi🙏

  • @mannanmalik6810
    @mannanmalik681011 ай бұрын

    hello mam i need recommendation to develop research project for phd related to genome editing. can you guide me in such.

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Sure. I will plan a live session

  • @likshitjaisinghani8818
    @likshitjaisinghani88188 ай бұрын

    what is the reason for degradation of the PAM site in the bacteriophage genome

  • @pavankalyan7899

    @pavankalyan7899

    Ай бұрын

    Bro did u find the answer coz even I have the same doubt

  • @Itz_Kanika0001
    @Itz_Kanika000111 ай бұрын

    Ma'am I'm not a mbbs student but I have a query that till how much time this technology would come to cure sickle cell anameia

  • @BaaYo

    @BaaYo

    11 ай бұрын

    Already some patients have been cured... search on Google.. I have seen a patient interview

  • @jitubhatt69
    @jitubhatt69 Жыл бұрын

    Mam ftnir pe video banaye pl.

  • @BaaYo

    @BaaYo

    Жыл бұрын

    Noted

  • @noorpk
    @noorpk Жыл бұрын

    If people are interested to see how to design experiments based on CRISPR-Cas9, can watch my tutorials.

  • @minato2363
    @minato23634 ай бұрын

    at 8:26 you asked question as ye bacteriophage ka genome kyun degrade nahi kr raha hai pls correct it mam bacteriophage ka genome hi to degrade kra hai abhi, uske baad sawal ye hona chahiye ki bacteria ka jo dna hai wo kyun degrade nahi hota is complex se jiska jawab hoga 1. lack of NGG sequence or motif called as Protspacer Adjacent Motif and lack of complementarity to bacteriohage's protoscapcer (spacer).

  • @BaaYo

    @BaaYo

    4 ай бұрын

    Yes i meant that only

  • @minato2363

    @minato2363

    4 ай бұрын

    @@BaaYo yaa that i got it but many can get confused hence i highlighted the same, and mam thanks for the video it was soo easy to understand in last moment thanks once again....

  • @shanubaba9321
    @shanubaba93216 ай бұрын

    Ise Hair loss ke genes ko bhi delete Kiya Jaa sakta hai kya

  • @BaaYo

    @BaaYo

    6 ай бұрын

    Mostly Hair loss gene ki vajah se nhi hota. But in case esa hota bhi hai to yes we can modify that gene

  • @shanubaba9321

    @shanubaba9321

    6 ай бұрын

    @@BaaYo In today's time, the problem of hair fall is due to genetic problems ..

  • @tayyabariaz2450
    @tayyabariaz245010 ай бұрын

    Ma'am ap bol rii hn r m write kr k notes bna ri Sath Sath ❤❤❤

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Good

  • @ultracraft1997
    @ultracraft19979 ай бұрын

    Mam please make a video on genetic vector.yac, bacteriophage, shuttle vector, replacement vector please.

  • @Vikas-hs9cl
    @Vikas-hs9cl7 ай бұрын

    My subject is applied physics but i am watching this video LoL (from pc microscopy to this )

  • @BaaYo

    @BaaYo

    7 ай бұрын

    I hope you are learning some new concepts 😀

  • @ph100k3
    @ph100k32 ай бұрын

    Mem aapko salo se dekh Raha hu kuch jyada hi vajan badh gayaa he aapka esa lag raha he 😢 you need diating

  • @kareem7974
    @kareem797410 ай бұрын

    😱 "Promo sm"

  • @footballshorts4294
    @footballshorts42948 ай бұрын

    Today is my presentation and its too complex why this topic is assigned for me band bajj gyi

  • @footballshorts4294

    @footballshorts4294

    8 ай бұрын

    Smjh to a gyi

  • @BaaYo

    @BaaYo

    8 ай бұрын

    @@footballshorts4294 bus jo samajh aaya vaise ka vaisa hi bol dena.. nervous ho isliye esa lag rha hai

  • @BaaYo

    @BaaYo

    8 ай бұрын

    All d best