CRISPR | CRISPR - CAS9 | Genome Editing Tool
What is CRISPR-Cas9?
CRISPR-Cas9 is a revolutionary gene-editing technology that has garnered significant attention in the field of genetics and molecular biology. It allows scientists to precisely modify the DNA of living organisms, offering a powerful tool to manipulate genes and potentially treat genetic diseases.
CRISPR-Cas9 Mechanism:
The CRISPR-Cas9 system works in several steps:
1) Adaptation: Bacteria encounter foreign DNA (e.g., from viruses) and incorporate fragments of this DNA into their CRISPR region.
2) CRISPR RNA Production: The CRISPR region is transcribed into CRISPR RNA (crRNA), which forms a complex with trans-activating CRISPR RNA (tracrRNA) to guide the Cas9 enzyme.
3) Targeting: The CRISPR-Cas9 complex scans the cell's DNA to find a sequence that matches the guide RNA (crRNA-tracrRNA complex).
4) Editing: Once the target DNA sequence is found, Cas9 cuts the DNA strand. This break can then be repaired through the cell's own repair mechanisms, which may introduce small mutations or allow researchers to insert or remove specific genetic material at the site of the cut.
Applications of CRISPR-Cas9:
CRISPR-Cas9 has numerous applications, including:
1) Basic research to study gene functions and understand biological processes.
Biomedical research for potential treatments of genetic diseases by correcting or modifying faulty genes.
2) Agricultural applications to develop crops with improved characteristics and resistance to diseases.
3) Biotechnology for engineering microorganisms to produce useful compounds or materials.
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#crispr #biotechnology #biology
Пікірлер: 111
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thank you for explaining so clearly
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It was a healthy one with alot of information in such shot duration. Thankfull❤
Mam, huge respect for you.. You made our tomorrow easy. I was totally confused about this technique but now 100% clear. Thanks
Apki jitni tarif kare, utni kaam h ma'am, 😍 thank you so much ❤️
@BaaYo
Жыл бұрын
❤
I was about to die without this class 😢... Thank you so much 👍
@BaaYo
4 ай бұрын
I hope all well now 😃
Thank you so much ma'am! It's just to the point explanation! ❤️❤️❤️❤️
@BaaYo
Жыл бұрын
❤❤
Thank you 👍
Teacher app jese hoto padhne or smjhne m b mja ata h maam ❤ #Nice teacher 🏆
very nicely explained. thank you mam
Thank you ma'am for such an easy explanation 🙏🙏...
@BaaYo
Жыл бұрын
Thank you Shivam
Thank you sooo much ma'am every video of yours are soo informative and useful thank you soo soo much💐😊👍🙏
@BaaYo
11 ай бұрын
My pleasure 😊
Thank uh for this concept vd
Very easy explanation now I understand each an every point.... thankyou so much ma'am......😊
@BaaYo
11 ай бұрын
Thanks dear... please share with your batchmates also...
Very well explained 👏
@BaaYo
Жыл бұрын
❤
Sukriya mam🌿
Excellent detail explanation
@BaaYo
Жыл бұрын
Thanks
First chanel found best for biotechnology
@BaaYo
Жыл бұрын
Thank u.. please share with ur batchmates also
Finally got it🎉....Thanks mam❤
best youtube channel i have ever seen .. thankyou mam
@BaaYo
4 ай бұрын
Thanks a lot 😊
Good 🎉 nice going...keep it up.
Excellent
Thanks
Thank you very much madam for nice delineation
Thank you ma'am ❤ 😊
@BaaYo
10 ай бұрын
Keep watching
Awesome
thank you mam
Nice ❤
Achha padati hai aap!!
Very useful session a lot of thanks ma'am
@BaaYo
11 ай бұрын
Thanks for liking
the video we were waiting for a nice explanation mam thank you......
@BaaYo
Жыл бұрын
Thank you
@ayushvishwakarma3568
Жыл бұрын
@@BaaYo mam please make video on bioinformatics
Thank you very much. As you have already said ki disruption krni hai toh DNA ki originality loose ho jayegi magar hum toh chahte ho hain ki Dna mein certain modification laayein toh originality toh wese bhi loose ho hi jati na Please explain it mam Btw your lectures are helping tools & mostly before examinations
Everything is temporary but mam's same fashion sense is permanent ❤
@BaaYo
Жыл бұрын
Haha
First viewer, very very nice explanation, no words left for praise ❤, thank you so much ma'am 💞
@BaaYo
Жыл бұрын
Thanks a lot
Nice lecture.
@BaaYo
Жыл бұрын
Thank you
Well define mam☺️🌹
Great explanation mam😊
@BaaYo
Жыл бұрын
Thank u
Ma'am or friend.where can i find previous years question paper without answer in it from internet of life sciences
Mdm yi crisper cas9 jb hm gene editing ki li use krti hy to wo kis trh ja ki ak gene ki sat ja ki bind kri ga q k gene ka complentery sequence to already gene mi hota hy .to crisper case ki zary editing krni si hm ligase use kri gy k wo ja k os complementary sequence ko ak dosry si seprat ki bad mi crisper ja ki wah pi bind kri ya koi aur mechnism hy
Very good mam
@BaaYo
Жыл бұрын
Thanks a lot
During cloning, how does the phage DNA integrate with the host cell genome and replicate if the bacterial genome contains a CRISPR locus?
@BaaYo
10 ай бұрын
Cas protein does this part.. integration
Welcome back ma'am
@BaaYo
11 ай бұрын
Thank you
@ambujpandey7610
11 ай бұрын
@@BaaYo it's my pleasure
Nice ma'am
@BaaYo
Жыл бұрын
Thanks
Mam please tell about crspr cas type 4
Very nice Mam.., and please make a video on Diagnostic
@BaaYo
10 ай бұрын
Sure I will
5.08 ke bad suddenly kaha shift hogyi ma'am i mean series wise nhi h lecture
Your teaching is good sister..... Please make more and more vedios on biotechnology for msc students
@BaaYo
Жыл бұрын
thanks
@harshitareddyharshitareddy9607
Жыл бұрын
How are sister
@harshitareddyharshitareddy9607
Жыл бұрын
@@BaaYowhat is your education status sis
Please tell me about more information.
Mam itna time bad video upload kiya ...please continue kriye ise please...regular post kriye mam
@BaaYo
Жыл бұрын
Yes
@BaaYo
Жыл бұрын
Sure 👍🏼, Thanks for watching!
Mam paramesium k morphology and reproduction p video daliye plz..
mam what is your source of information as i am working on topic "crispr in infection control" . It would be helpful if you can share some information related to this. Thankyou
@samyakmoon7235
Жыл бұрын
will you share with me if any articles or paper you have got, cause our topic is similar
how is gene editing? Can we have white akın, blonde hair, blue eyes without Being reborn? İs this true? İs such a thing really possible? If so, when will it be operational worldwide? İs it okay to use this for health or cosmetic purposes?
Yekab launch hoga aur cost kitna hoga
Hi mam bsc 5th sem ka zoology kab padhayegi🙏
hello mam i need recommendation to develop research project for phd related to genome editing. can you guide me in such.
@BaaYo
11 ай бұрын
Sure. I will plan a live session
what is the reason for degradation of the PAM site in the bacteriophage genome
@pavankalyan7899
Ай бұрын
Bro did u find the answer coz even I have the same doubt
Ma'am I'm not a mbbs student but I have a query that till how much time this technology would come to cure sickle cell anameia
@BaaYo
11 ай бұрын
Already some patients have been cured... search on Google.. I have seen a patient interview
Mam ftnir pe video banaye pl.
@BaaYo
Жыл бұрын
Noted
If people are interested to see how to design experiments based on CRISPR-Cas9, can watch my tutorials.
at 8:26 you asked question as ye bacteriophage ka genome kyun degrade nahi kr raha hai pls correct it mam bacteriophage ka genome hi to degrade kra hai abhi, uske baad sawal ye hona chahiye ki bacteria ka jo dna hai wo kyun degrade nahi hota is complex se jiska jawab hoga 1. lack of NGG sequence or motif called as Protspacer Adjacent Motif and lack of complementarity to bacteriohage's protoscapcer (spacer).
@BaaYo
4 ай бұрын
Yes i meant that only
@minato2363
4 ай бұрын
@@BaaYo yaa that i got it but many can get confused hence i highlighted the same, and mam thanks for the video it was soo easy to understand in last moment thanks once again....
Ise Hair loss ke genes ko bhi delete Kiya Jaa sakta hai kya
@BaaYo
6 ай бұрын
Mostly Hair loss gene ki vajah se nhi hota. But in case esa hota bhi hai to yes we can modify that gene
@shanubaba9321
6 ай бұрын
@@BaaYo In today's time, the problem of hair fall is due to genetic problems ..
Ma'am ap bol rii hn r m write kr k notes bna ri Sath Sath ❤❤❤
@BaaYo
10 ай бұрын
Good
Mam please make a video on genetic vector.yac, bacteriophage, shuttle vector, replacement vector please.
My subject is applied physics but i am watching this video LoL (from pc microscopy to this )
@BaaYo
7 ай бұрын
I hope you are learning some new concepts 😀
Mem aapko salo se dekh Raha hu kuch jyada hi vajan badh gayaa he aapka esa lag raha he 😢 you need diating
😱 "Promo sm"
Today is my presentation and its too complex why this topic is assigned for me band bajj gyi
@footballshorts4294
8 ай бұрын
Smjh to a gyi
@BaaYo
8 ай бұрын
@@footballshorts4294 bus jo samajh aaya vaise ka vaisa hi bol dena.. nervous ho isliye esa lag rha hai
@BaaYo
8 ай бұрын
All d best