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Pour Plate Technique for Colony Counting_A Complete Procedure (Microbiology)
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. So it is a very important method for a microbiologist.
This video presents a detail and complete procedure for colony counting of bacteria and fungi by Pour Plate Technique.
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Пікірлер: 39
Mam is VRBA media in this vidio is Autoclave? because VRBA is not autoclave
@MicroChemsExperiments
Жыл бұрын
Watch the video again. I just talked about general procedure media preparation. I said, Either you have to boil or you need to autoclave.
@Muhammadahsan-cr8zh
Жыл бұрын
@@MicroChemsExperiments Now its clear
Why do you turn the plate upside down?
@paruljoshi2659
2 жыл бұрын
to collect water of condensation on the lid.
@MicroChemsExperiments
2 жыл бұрын
Exactly
Can I use this method with a complex sample (like soil or bee pollen) to first culture and count the colonies with serial dilutions and then use the cultures from the serial dilutions to isolate the different resulting colonies to create pure cultures?
@MicroChemsExperiments
3 жыл бұрын
Yes. You can
@alejandropm6017
3 жыл бұрын
@@MicroChemsExperiments thanks, colonies will grow inside the agar or in the surface?
@MicroChemsExperiments
3 жыл бұрын
Inside the agar media
@utechinternational
25 күн бұрын
@@alejandropm6017 you will get the colonies on the surface and sub-surface of the agar media.
Thank you ma'am 😊
@MicroChemsExperiments
2 жыл бұрын
You are welcome
Please suggest me which media is suitable for fecal coliform test by pour plate method. And how we can prepare this media?
@MicroChemsExperiments
2 жыл бұрын
M-FC agar.
Excellent 👍
@MicroChemsExperiments
Жыл бұрын
Thank you
Maam , can we use this method for Nutrient agar ?
@MicroChemsExperiments
Жыл бұрын
Yes. You can
Could we use this method to test the viability of probiotics in ice cream?
What formula will we use to calculate CFU/g by this method? What will be the dilution factor?
what kind of agar base you're using in this tutorial?
@thazinphyu1325
2 жыл бұрын
For milk and Milk product and some product, we can use Violet Red Bile Agar
@MicroChemsExperiments
2 жыл бұрын
VRBL Agar
everything is good but y fake accent
what about colony forming units please explain
@MicroChemsExperiments
2 жыл бұрын
Please take help from Google database
TQ
@MicroChemsExperiments
Жыл бұрын
You are welcome
I want to ask. Stomacher bag are use or not in this experiment ?
@MicroChemsExperiments
3 жыл бұрын
We didn't use stomacher bag. Because no need of use in this technique.
@nurulizzah6248
3 жыл бұрын
@@MicroChemsExperiments seriously . Because our lab manual there is written has a stomach bag
@MicroChemsExperiments
3 жыл бұрын
@@nurulizzah6248 Generally, tomacher bag is used for sample preparation. But in this video, I didn't show any sample preparation. I just explained the procedure of pour plate method. That's why I didn't use stomacher bag in this video.
@MicroChemsExperiments
3 жыл бұрын
@Kylen Cannon Please don't discuss about any irrelevant topic here. Thanks for your coperation.
@MicroChemsExperiments
3 жыл бұрын
@Devin Jamari Please don't discuss about any irrelevant topic here. Thanks for your coperation.
very bad background music
@MicroChemsExperiments
Жыл бұрын
Sorry for that.
Your from mam
@MicroChemsExperiments
2 жыл бұрын
Thanks