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#streak #plate #isolation #pure #alphagenomics
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The streak plate technique is used for the isolation into a pure culture of the organisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. Some individual bacterial cells are separated and well-spaced from each other.
As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually, by the third or fourth quadrant, only a few organisms are transferred which will give discrete colony forming units (CFUs).
Principle of Streaking
The sample/inoculum is diluted by streaking it across the surface of the agar plate. While streaking in successive areas of the plate, the inoculum is diluted to the point where there is only one bacterial cell deposited every few millimeters on the surface of the agar plate. When these lone bacterial cells divide and give rise to thousands and thousands of new bacterial cells, an isolated colony is formed. Pure cultures can be obtained by picking well-isolated colonies and re-streaking these on fresh agar plates.
Materials required:
A source of bacteria (stock culture, previously streaked agar plate, or any other inoculum)
Inoculation loop
A striker/lighter
Bunsen burner
Lysol (10%v/v)
Agar plate (nutrient agar or any other agar medium)
Paper towels
Related Tag:
#Streak
#PureCulture
#Microbiology