PI3k/AKT/mTOR Pathway
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The Akt signaling pathway or PI3K-Akt signaling pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are PI3K (phosphatidylinositol 3-kinase) and Akt (protein kinase B).
Initial stimulation by one of the growth factors causes activation of a cell surface receptor and phosphorylation of PI3K. Activated PI3K then phosphorylates lipids on the plasma membrane, forming second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP3). Akt, a serine/threonine kinase, is recruited to the membrane by interaction with these phosphoinositide docking sites, so that it can be fully activated.Activated Akt mediates downstream responses, including cell survival, growth, proliferation, cell migration and angiogenesis, by phosphorylating a range of intracellular proteins. The pathway is present in all cells of higher eukaryotes and is highly conserved.
There are multiple types of phosphoinositide 3-kinase but only class I are responsible for lipid phosphorylation in response to growth stimuli. Class 1 PI3Ks are heterodimers composed of a regulatory subunit p85 and a catalytic subunit p110, named by their molecular weights.
The pathway can be activated by a range of signals, including hormones, growth factors and components of the extracellular matrix (ECM).[5] It is stimulated by binding of an extracellular ligand to a receptor tyrosine kinase (RTK) in the plasma membrane, causing receptor dimerization and cross-phosphorylation of tyrosine residues in the intracellular domains. The regulatory subunit p85 binds to phosphorylated tyrosine residues on the activated receptor via its Src homology 2 (SH2) domain. It then recruits the catalytic subunit p110 to form the fully active PI3K enzyme. Alternatively, adaptor molecule Grb2 binds to phospho-YXN motifs of the RTK and recruits p85 via Grb2-associated binding (GAB) scaffold protein.
Пікірлер: 45
Thanks author for the video. One thing to add - PDK1 phosphorylates Akt protein at Threonine (T308) residue not Tyrosine (Y).
Excellent video, please note than PI3K can also be activated directly by receptor tyrosine kinase without involving Ras-GTP.
I was confused in this topic and here you are with the video thank you so much👏🌟
@hussainbiology
3 жыл бұрын
thanks for appreciation..Glad to know that it helps ✌️
Very good video. You Indian guys are the best when explaining molecular stuff, almost all the stuff I understood via KZread was explained by Indian youtubers. People explaining the stuff in American English somehow don't have the skill to explain stuff properly, don't know why.
@hussainbiology
3 жыл бұрын
thanks for appreciation..Glad u like my stuff
Insulin receptors exist as diners and when ligands binds two diners bind to each other to form tetramer
thanks for the video, one thing i would change is that I would call tyrosine 308 on PKB Y306, not T308
@sudharmabanerjee6699
2 жыл бұрын
It should be Threonine (Thr or T), not Tyrosine. He was confused while explaining.
Goood.....waiting
@hussainbiology
3 жыл бұрын
✌️✌️
वेटिंग फॉर this वीडियो
Your videos are simply awesome.....Please try to make more Dev. Bio . videos as CSIR NET exam is on the verge. thank you.
@hussainbiology
3 жыл бұрын
thanks Ashish for appreciation. Glad you like it...
We cant write this for pi3 pathway
Sir, please tell me that can i do MSc in genetic engineering after my Bsc in microbiology ? Or what else is required to pursue MSc in genetic engineering?
@krishnendukundu6446
3 жыл бұрын
For msc biotech....u will get broad range..in industry as well as research
أريد شرح بالعربي بالنسبه لموضوع PI3K-AKT
1:29 does insulin bind RTK or insulin growth factor?
@guptashivam6399
3 жыл бұрын
Insulin as a ligand bind to rtk receptor
Sir can you please tell me about the best book for such pathways????
@hussainbiology
2 жыл бұрын
Lodish Cell Biology , Alberts Cell Biology
Good work. Need to correct few info.
@hussainbiology
2 жыл бұрын
thanks... yes please
RTk form dimers upon engagement with there ligand but the only exception lignad is insulin ?? did iam right ?
@ikrambenali9452
3 жыл бұрын
Yes
Thank you so much. #bussymed
@hussainbiology
3 жыл бұрын
You are welcome
Sir ,r u also available on telegram
@hussainbiology
3 жыл бұрын
No dear...
@hussainbiology
3 жыл бұрын
Available now
@evamadaan4933
3 жыл бұрын
@@hussainbiology @hussainbiology is this the channel
@hussainbiology
3 жыл бұрын
@@evamadaan4933 i am new to Telegram.. What i know is my id only.. and yes as Hussainbiology 1253420376
@hussainbiology
3 жыл бұрын
@@evamadaan4933 Yes Eva
Sorry i'm late. I am pretty certain that PDK2 does not phosphorylate S473, and that is instead mTORC2. Furthermore, you never care to explain how PDK1 and 2 are activated by PIP3
@kennethdaniella-ab7963
Ай бұрын
yep he did not explain it but you can read it at ncbi website, there is a ton of research paper there related to this
Aree kehna kya chahte ho bhaiii 🙄 pura ghuma diya hindi me hi bol le
@hussainbiology
3 жыл бұрын
Hindi mai samajna hai to ye dekho kzread.info/dash/bejne/gn-pqcivmZWdlJc.html