The amount of professionalism shown in this video is awesome.

@theeargasmchannel

4 жыл бұрын

can't relate lol

@najwaasri4073

3 жыл бұрын

) poppa

@eli_farar

2 жыл бұрын

you are correct

idk what youtube algorithm is doing but its already far beyond human comprehension. I watched the entire thing even though i dont even own or use this stuff.

@bashkillszombies

4 ай бұрын

Why don't you? You should get them! You're missing out! Think of all the fluids you could pipette into vials! And then pipette them out of those vials into other vials! Tbh I reckon this should be an olympic sport. >_> I'm kidding, I'm kidding. But it can be useful for routine testing of things. Are you a gardener? Fancy hybridizing or cloning a specific plant to improve it or make it resilient to something? NPK measurements of hydrponic solution benefit from good lab technique like this, note it all down, and then perfect your mix!

@froschreiniger2639

4 ай бұрын

@@bashkillszombiesHaha olympic gold medal for pipetting sounds kind of nice. But yes i agree, its useful to know some day. I have actually grown some oyster mushrooms once and that was quite hard since everything had to be sterile. I have grown some plants too. Who knows maybe some day i can use this.

Damn, she handle those pipettes perfectly with no tremors

Thank you. Will be using this as a reference video for my trainees! Very clear and valuable resource. Love my eppendorf tools too!

Loved this video, extremely helpful and filled with a lot of information no one else provides in a very short video. Thank you

Thanks for this video, I need to learn about biosafety for my job. I had no idea what pipetting was. lol

thanks for the explanation on pre-wetting

this showed me what to do correctly so I don't mess up the tool or experiment

I really love you. You are an amazing teacher. No one has never explain it so accurately than you. Thank you so much.

@venkatm8020

3 жыл бұрын

Abba chaa

Thank you for this tutorial.

Best pipetting video. Ever.

@andersmathiasrnneberg3547

6 ай бұрын

awesome

The way she squeezes that pipette with professionalism is incredible especially that slow motion.

@sebastien8415

Жыл бұрын

True. Its almost erotic the handling of that Pipette.

@aura3n

Жыл бұрын

@@sebastien8415 this is about a pipette you horndog

@sebastien8415

Жыл бұрын

@@aura3n you don't get my humor, right?

@andersmathiasrnneberg3547

6 ай бұрын

@@sebastien8415 :(

Thank you for your video.

Thx, Im sure now, I have done it correctly the last two years

This is work out me thanks 😊👍👍

thk's so much

Very nice explanation

her voice is so relaxing...

Thank you

This video showed a good up-close demonstration 🙂

My father also works in eppendorf

Why is this on my recommendation? Well, additional knowledge always wellcome

So why is this in my recommendations

@GodOfPizza

4 жыл бұрын

Same, dafuq

@mike5214

4 жыл бұрын

its interesting

@deshengriview

4 жыл бұрын

if u studying or working in biomedial field it's necessary to know this

@user-nh6em4qq3q

3 жыл бұрын

Yeah why

@angelusvastator1297

3 жыл бұрын

If you're majoring in something related to medicine, chemistry, biology, you'll understand why.

Fabulous ☺️☺️

Super madam.. 👌👌

1st of all why was this recommended to me? 2nd of all I am on vacation right now, I didn't really want to have anything to do with work related things. 3rd and last point. I use the pipette frickin everyday in the lab, for years by now, I should know how to use it. So tell me what has been going on in youtubes mind?????

Fantastic

Nice video

I use a pipette every day at work, but KZread recommended and here I am

Informative

Would you suggest completely ejecting during aspiration with an electronic eppendorf pipette as well?

This is so amazing

the ultamate, "thats what she said," video doesnt ex.....

@eppendorf Is there any written guide for this? If there is, can you please send me the link?

Thanks akka

Good very good

How you stay relaxed at the second stop tho,?

So much for that! I though I was going to learn how to better load weed into a bong!

Please use this thread to add your own pipetting tips too. Thank you :)

Wow!

are those tips universal for this model and other mfg'rs models too ?

@nicorome7154

4 жыл бұрын

Eppendorf tips are mostly universal except for certain manufacturers like Rainin but matching manufacturer tips with each pipette will produce the greatest accuracy and precision.

Tq

Does the depth at which a tip is sent into the "sample" effect the accuracy as small portion is stuck outside esoevialky for yge 20 ul pipettes?

This German chick speaks better English than I do.

Bruh im always shaking when i put the caps on

Is there a way to accurately pipet cell suspensions? There are always residuals on the tips. Cheers!

@DynamiteProd

Жыл бұрын

You have to pipette in the exact same way. If there is any residual it’s probably because it’s not liquid only but some of the cells or their debris might attach to the tip.

Great 👍 mam...👏thanks for this video, can you tell me what's the difference between ml and meu l

@histephenson007

Жыл бұрын

ml = 10 ^ - 3 (milli litre) Meu L = 10 ^ - 6 (micro liter) Therefore, 1000 Meu L = 1ml

How about reverse pipetting?

It doesn't show how to adjust the amount

is accurate delivery affected by altitude ? here at mile high colorado water boils less than 100 C.

@christineteng980

3 жыл бұрын

I would think so, as air pressure affects accuracy

Pipe up!!

0:45 "there's no need for force..." your mileage may vary...

Nice

@anisheshdas362

2 жыл бұрын

ok. I think the same too. Do you have this instrument in your college lab?

@rashmiranjanmohanta5693

2 жыл бұрын

@@anisheshdas362 I think the equipment in our college is little small

@anisheshdas362

2 жыл бұрын

@@rashmiranjanmohanta5693 hehe

What is the good immersing depth for good calibration?

@DynamiteProd

Жыл бұрын

As long as the tip is in the liquid it’s fine.

Always wear eye protection 😎

@mike5214

4 жыл бұрын

Alexander Cook PPE 😎

When dispensing the liquid does the pipette tip need to be against the wall of the test tube? And must the pipette be vertical position when dispensing the liquid? Thank you!

@rachelmonk3453

2 жыл бұрын

I think when dispensing the liquid, the pipette needs to be at an angle of between 20-40 degrees so not completely vertical

Focus on the processing of pipette tips by international brands, high-end customization, quality assurance, welcome to consult

Is anyone else watching this because practicals have been banned due to corona?

I wished she would've shown both ends of the pipette when measuring. It gives room for misinterpretation if you don't show both actions at the same time.

what does sasperated mean

@gabeorgabriel

3 жыл бұрын

aspirate: basically suck up thru a tube

What about wiping down the sides of the pipette with a tissue?

@Cytogreg

5 жыл бұрын

If the pipette tip is clean and attached from the original container and not immersed into the sample beyond the minimum necessary depth, there is no need to wipe the tip. Wiping only increases the opportunity of absorbing the sample from the tip and rendering the sample volume inaccurate.

@zeynepipek2902

3 жыл бұрын

You do not need to wipe the tip. Also with the small volumes you pipette with these at times it could greatly affect your results. But since these tips are not wiped you should pay attention to immerse your tip just enough for it to take up the liquid Pushing to the second pressure point allows all the liquid within the tip to come out. Normally wiping is only done with large glass pipettes and even then the tissue shouldn't touch the tip as to not suck out liquid accidentally

Hi molecular bio 2 period people

1. What if the angle of immersion & ejection is not followed properly? 2. What causes bubbles while pipetting?

@BoluwajiPieters

2 жыл бұрын

If the angle of immersion is not followed properly, there'll be inaccuracies in the amount of fluid withdrawn as well as drawing up of air bubbles especially if the fluid withdrawn is of low volume (for example, withdrawing 0.2ml from a 0.35ml sample.) If the angle of ejection is not 20-45° and in contact with the deposit vessel walls, there could also be erroneous delivery of such sample. Air bubbles are caused by different factors including poor immersion depth (the commonest), the type of fluid, using the wrong pipette tips, releasing the plunger too fast and some other factors that depends on the skills of the person using the micropipette.

@p.m.satyanarayana8505

2 жыл бұрын

@@BoluwajiPieters Thank you! How to tighten the tip ejector? Any suggestions please.

@BoluwajiPieters

2 жыл бұрын

@@p.m.satyanarayana8505 Choose the appropriate tip. When putting on the tip, apply a little pressure on the tip to make sure it fits perfectly to prevent any spaces around the borders of the tip and the micropipette. The pressure should be enough to make the tip stay on the pipette and also enough to make the tip fall off when it's time to eject the tip using the ejector slide on the side of the pipette.

@p.m.satyanarayana8505

2 жыл бұрын

@@BoluwajiPieters The metal part that lines the micropippette gets loose sometimes. So properly using the tip can fix the problem?

@BoluwajiPieters

2 жыл бұрын

@@p.m.satyanarayana8505 Then that's probably a mechanical problem. If I understand you well. You can use newer pipettes or send the said pipette for repair if the tip isn't gripping the pipette because the metal part is loose..

I'm sorry, but why exatly do you have to repeat the initial aspirations two to three times?

@alexklein8333

2 жыл бұрын

There's air between the liquid and the piston, so aspirating a few times lets surface molecules evaporate off the surface and saturate that air. This way you don't lose anything potentially significant to evaporation into that space. That's the explanation on ThermoFisher's site. Maybe it's most significant for very small volumes?

At the end she forgets to mention pre wetting of the pipette tip...

What about the precision rating? Whats the difference between electronic & manual pipette?

@VT-mw2zb

4 жыл бұрын

That's why you calibrate them.

@zeynepipek2902

3 жыл бұрын

It actually also differs from manufacturer to manufacturer. And depends on the range of the pipette. Reference precision scales can be found in the relevant protocols on the companies website (look up your pipette model and then the calibration protocols). You'd still need to check your pipettes in regular intervals and send them in if your result is outside the referenced precision scale

Didn't know what this was and was curious - saw the beautiful lady with the sensational voice and decided to say......after 3:09mins I didn't know know what this was all about. Bye for now Dr. Henke.

I just realised i stopped at ball pippetes, while scientists use fucking computer controlled pipettes.

🌸🌸🌸

Why no safety goggles?

Oh man I've been doing it wrong. Vertical aspirate it first Don't over dip the pitpete tip into the liquid

I need PDF file can. U send me

I don’t know why I watched this entire thing

Hadir 050

where can I buy this woman?

Don't know why this was recommended, but I'd pipe that lol

The sexual tension is real

@animepabu5526

3 жыл бұрын

Lmfao

@angelusvastator1297

3 жыл бұрын

She's very professional and neat.

@jefferypinley4336

2 жыл бұрын

I'd probably let her handle my pipette any day

Intelligent women are hot!

wait am i the only one that actually looked for this?

This was great…the sexual tension in this video could rival the surface tension on that liquid. Entertaining and informative…excellent job.

212K people wanna learn how to pipe that’s sad

@israelarellano8335

4 жыл бұрын

Not enough people take the time to learn this important skill.

@rinamizelia7298

3 жыл бұрын

It's not enough people until not every director, actor and writer of a sci-fi movie or show have seen it, because they show pipetting very wrong…

She's hot

el0n mus k sax tape

Thank you