How do you use a Micropipette? A step-by-step guide!

Ғылым және технология

The Micropipette is the most important tool in the molecular biology lab - but how do you use one?
0:00 Introduction
0:19 What is a micropipette?
0:52 Step 1: Choose the right micropipette
1:46 Step 2: Set the volume
2:50 Step 3: Use a fresh tip
4:09 Step 4: Draw up and dispense the liquid
5:35 Step 5: Eject the tip
This practical guide introduces you to the basics of how to use a Micropipette with advice for things to avoid and Pro-Tips so you can hit the ground running.
Cartoon Illustrations by Michelle Lotker
This video is copyright Jayme Dyer, published under the Creative Commons CC-BY SA 4.0 license.
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Пікірлер: 97

  • @angiem2192
    @angiem21922 жыл бұрын

    You’re amazing. 10 years in biology. And not one professor has ever said THE SECOND STOP IS FOR DISPENSE ONLY. this fixed every experiment I have for micro this semester thank you

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Yay I'm glad my video was helpful!

  • @bnrakesh9156

    @bnrakesh9156

    Жыл бұрын

    ​​​@@YouTooBioYes ma'am your teaching is super🙏🙏🙏 You are enhancing both the theoretical n practical knowledge about the topic. Thank you so much ma'am

  • @prophetlocs7040
    @prophetlocs70403 жыл бұрын

    You’re video has been the best in explaining everything in detail yet very easy to understand, thank you.

  • @mubrra4748
    @mubrra4748Ай бұрын

    The attention to detail is fantastic here .....every possible point is covered with the utmost clarity 😮

  • @OffGridInvestor
    @OffGridInvestor2 ай бұрын

    FAR better than the first video I watched. It couldn't explain the 2 stops properly. Another one of those videos where the person explaining it is like he's explaining it to someone who already knows.

  • @jamiehollanders3259
    @jamiehollanders32593 жыл бұрын

    Clear, concise and fun video! Perfect for students and beginners like myself. Thank you!

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Glad it was helpful!

  • @dannachavez275
    @dannachavez2753 жыл бұрын

    this has been so helpful. thank you so much. I won't be walking in clueless into my intro bio lab now.

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Glad it was helpful!

  • @fishfish20
    @fishfish204 ай бұрын

    I'm liking your channel. I'll be working on a lab soon, so I'm familiarizing myself before the training. 😂

  • @queenesther.chukwuka
    @queenesther.chukwuka5 ай бұрын

    You’re an amazing Teacher ma’am. I love the demonstrations ❤

  • @crochetbyhafsaaa
    @crochetbyhafsaaa Жыл бұрын

    this video legit saved me before my OSPE exams, thankyou🥰

  • @bluesteno64
    @bluesteno642 жыл бұрын

    Just had the pleasure of watching this in my AP bio class. I laughed quite a few times! Thank you for putting this video together

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Glad you liked it!

  • @SaraGreen-xr3tl
    @SaraGreen-xr3tl Жыл бұрын

    What a fantastic and concise explanation. Thank you!

  • @anime991
    @anime9912 жыл бұрын

    This is very helpful. I have to teach some new undergraduate students how to use micropipets but I was having a hard time figuring our how to accurately convey what I know in a way that makes sense to people with no lab experience

  • @osmirod1
    @osmirod12 жыл бұрын

    I love teachers like you. Thank you 😊

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Thank you! 😃

  • @DrFodz
    @DrFodz3 жыл бұрын

    That was a very helpful, informative, fun, and memorable video! Clearly a lot of thought and work has been put into it and it SHOWS Love your style!

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Thanks so much - glad it was helpful!

  • @1313babinski
    @1313babinski5 ай бұрын

    The best I’ve seen you covered pretty much all possible question great great video!!!!!

  • @imperpekto12ify
    @imperpekto12ify3 жыл бұрын

    I like how you made this video so friendly and funny at the same time. Thank you for making the video.

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Yay glad you found it to be approachable!

  • @naomia9848
    @naomia9848 Жыл бұрын

    thank you so much! I have a micropipette practicum today, this was super helpful!

  • @himilyiasriprabagaran2691
    @himilyiasriprabagaran26919 ай бұрын

    Loved the way you explained it in detail way simpler. Thank you!!

  • @Ceecee_00000
    @Ceecee_000003 жыл бұрын

    Very Informative! Thank you

  • @bulbulshaikat4417
    @bulbulshaikat4417 Жыл бұрын

    Very well said. Thanks from Bangladesh 🇧🇩

  • @umba9610
    @umba9610 Жыл бұрын

    Thank you for this video. It is so helpful. I have to learn this immediately

  • @jamesdeancafino1240
    @jamesdeancafino12403 жыл бұрын

    I really liked the way you explained micropipettes. Thank you.

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Glad you enjoyed it!

  • @BChew-ku9he
    @BChew-ku9he3 жыл бұрын

    Thank you for your video!!! You helped me with my lab homework!! ❤️❤️

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Hooray! Glad it was helpful

  • @christinephilip3849
    @christinephilip38492 жыл бұрын

    Really helped me in doing my experiments properly 👍🏻👍🏻

  • @Sp-fe1eo
    @Sp-fe1eo2 жыл бұрын

    thanks for the video...best explaining...easy to understand and helped me a lot...love this channel ❤

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Thanks!

  • @madi6880
    @madi68802 жыл бұрын

    Tomorrow is my first in-person second-year lab, so I was looking to refresh my memory on how to use micropipettes, and this video was perfect! It was so easy to understand and all of the visuals and animations helped so so much. I was worried about not remembering how to use the micropipettes, as I never got to physically use them in my first-year labs due to everything being online because of COVID, but now I can say I'm fairly confident, and even excited to use them, all because of this video :)

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Hooray! Glad the video was helpful. Good luck in lab!

  • @madi6880

    @madi6880

    2 жыл бұрын

    @@YouTooBio Thank you!

  • @alitahir7471
    @alitahir7471 Жыл бұрын

    I watched too many videos but this one is perfect like the best thank u ma'am ❤️

  • @primalshasandunigamage5682
    @primalshasandunigamage56822 жыл бұрын

    Wow..ur explanation is clear and amazing 😘😘

  • @sydneykate
    @sydneykate2 жыл бұрын

    Thank you for this video!

  • @o.m9514
    @o.m95142 жыл бұрын

    Cool! Thank you, your video makes learning about an equipment, easy, breasy and fun!

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Thanks!

  • @DarkSoul-du1ck
    @DarkSoul-du1ck3 жыл бұрын

    The video is very useful and understandable. Thank you very much !

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    Thanks! Glad it's helpful.

  • @snarlymacsnarls488
    @snarlymacsnarls4882 жыл бұрын

    This is such an amazing tool, thank you for such a thorough and entertaining video 💪🏻😄

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Thanks! ❤️

  • @faheenan385
    @faheenan3853 жыл бұрын

    Thank you so much, u made my day 😊😊

  • @vyankateshzambare1440
    @vyankateshzambare1440 Жыл бұрын

    I liked how you added those dramatic Eppendorf tubes reactions in the video🤣

  • @debjitbanerjee8512
    @debjitbanerjee85122 жыл бұрын

    Please make more such amazing videos...

  • @Sabridemir1981
    @Sabridemir1981 Жыл бұрын

    great video!!!

  • @khairatallyamour5117
    @khairatallyamour5117 Жыл бұрын

    Understand...enjoyable

  • @nalin_gunathilaka4067
    @nalin_gunathilaka40672 жыл бұрын

    excellent explanation

  • @chinememila6998
    @chinememila6998 Жыл бұрын

    You are so awesome...you made this fun and I understood everything thank you 😊

  • @YouTooBio

    @YouTooBio

    Жыл бұрын

    Yay! Glad it was fun

  • @cookwithme1698
    @cookwithme16982 жыл бұрын

    Very helpful video thanks

  • @lbe1932
    @lbe1932 Жыл бұрын

    Thank you very appreciated :)

  • @lorazam_
    @lorazam_3 жыл бұрын

    The best video I have ever seen

  • @mohammedidreesshafi4244
    @mohammedidreesshafi42443 жыл бұрын

    Thank u

  • @user-nu4gn3nx8m
    @user-nu4gn3nx8m8 ай бұрын

    thank you so much...

  • @arianmahi1915
    @arianmahi19152 жыл бұрын

    So helpful for me☺ really cool

  • @nourhanelsherif2787
    @nourhanelsherif27872 жыл бұрын

    Thank you 🌷🌷

  • @aslhanv6531
    @aslhanv65312 жыл бұрын

    such an amazing video that really teach me cool tips

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Glad you liked it!!

  • @Alchemist_171
    @Alchemist_1713 жыл бұрын

    Thank you so much!!

  • @YouTooBio

    @YouTooBio

    3 жыл бұрын

    You're welcome!

  • @fizarafique8150
    @fizarafique81502 жыл бұрын

    So nice of you mam amazing lecture 😊

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    Thanks a lot 😊

  • @habibullah1090_
    @habibullah1090_ Жыл бұрын

    Awesome

  • @shrishtinarang8650
    @shrishtinarang86504 ай бұрын

    Very nice video You are soo cool. Wish could take you to my lab . Need this kind of cool

  • @Felix-dt1tu
    @Felix-dt1tu4 ай бұрын

    Amazing video, really liked everything, especially the tips and the warning being so clear, BUT please mention somewhere that at 2:18 you made a mistake on the biggest pipette saying "0 6 5" equals 650qm, please make a note in the top comment. For the rest; amazing Thanks a lot

  • @gheedalfawaz370
    @gheedalfawaz3702 жыл бұрын

    You are an angel

  • @beyuorichard968
    @beyuorichard968 Жыл бұрын

    Good

  • @kiarajelo87
    @kiarajelo872 жыл бұрын

    love this! do u have a videoo saying the right way (angle) to pipette?

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    I don't! What is your vision for that video?

  • @keyspark
    @keyspark4 ай бұрын

    after watching this video, I feel like a mircopipetting pro

  • @tchistermorrelebissa8628
    @tchistermorrelebissa86288 ай бұрын

    Good demonstration, Madam, but could we know which graduation is considered for a given micropipette? I mean, for example in one pipette there are three (03) numbers appear and which number to consider?

  • @jocker_boys
    @jocker_boys Жыл бұрын

    You are very nice 🙂🙂🙂🙂❤️❤️❤️❤️❤️❤️

  • @michaeldurairajs6696
    @michaeldurairajs66963 ай бұрын

    Does the depth at which a tip is sent into the "sample" effect the accuracy as small portion is stuck outside especially for the 20 ul pipettes?

  • @Ahmed-zu9yv

    @Ahmed-zu9yv

    3 ай бұрын

    Yes.the Depth is very important. Its proportional directly to the volume you have to draw.

  • @pandjnixon
    @pandjnixon Жыл бұрын

    Great teaching video ! - You no doubt can recycle the used tips by autoclaving ?

  • @YouTooBio

    @YouTooBio

    Жыл бұрын

    In theory, yes but most people don't. There's no automated way to clean and sort them back into tip boxes, so it's practically very expensive (labor) to clean, autoclave and sort.

  • @pandjnixon

    @pandjnixon

    Жыл бұрын

    @@YouTooBio OK - Thanks Jayme.

  • @Mark-zo4zh
    @Mark-zo4zh2 жыл бұрын

    I have a bunch of these how do I sell them

  • @osmirod1
    @osmirod12 жыл бұрын

    I love you.

  • @AleForFun
    @AleForFun Жыл бұрын

    How without a stand for both micropipette and tips?

  • @polo8601
    @polo860111 ай бұрын

    Ma'am let's say we need to pipette out 1500µl...and we have a P1000 micropipette.....then first we have to set 1 0 0 to pipette out 1000µl and then AGAIN we have set the volume display as 0 5 0 to pipette out 500µl more....total 1000+500=1500µl....is that the correct procedure mam ?

  • @YouTooBio

    @YouTooBio

    11 ай бұрын

    You can do it that way, but it would be faster to do 750ul twice.

  • @IdiotWithEducation
    @IdiotWithEducation2 жыл бұрын

    If I were to use this to extract rotifers from a slide, what sized tip would I need?

  • @YouTooBio

    @YouTooBio

    2 жыл бұрын

    I would try a p1000 tip. If the opening of the tip is too small, cut a little piece of the tip off with a razor blade to make the opening a little bigger.

  • @IdiotWithEducation

    @IdiotWithEducation

    2 жыл бұрын

    @@YouTooBio thanks so much!

  • @tasneemabdulsattar7417
    @tasneemabdulsattar74173 ай бұрын

    Isn't 065 65ul and not 650 ul?

  • @jcdyer3
    @jcdyer33 жыл бұрын

    Two viewers were looking for macropipettes.

  • @juliusmaime5959
    @juliusmaime59593 жыл бұрын

    careful pi petting ,check tutorial for fixed pi petting .

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