Amazing mam ... Best explaination ever for genetics...👍

Ma'am will you please explain us "red-white selection" of YAC.

Very very important and informative video. Thank you

thanx didi..whenever there are doubts , i come to ur channel

The way you teach & describe the molecular events is really fascinating & so easy to visualise. Keep up the great work mam. 😇😇😇👍👍👍👌👌👌💜💜💜

@BaaYo

4 жыл бұрын

Thanks a lot Subhamay 😊

This was by far the best video on bluewhite screening. I was really confused and nervous about this topic but you made me understood sooo well. Best teacher ever.

You had the best explanation Thank-you ma'am

best video to understand this topic❤

Thank you so much 💕 ma'am. You give me the concept that i never understood before 💯

Best teacher on KZread ❤️

Thanks a lot mam, your explanation is great, & finally give a clear concept.

Great teaching

Thnks mam 🥰 🙏 .stay home stay safe . N we are hpppy u 2 say also safe .....

Thanks ma'am, you helped a lot. Your video gave me a proper concept.

Well explained ✌️nice🌹

Well explained! 😍

thankyou mam the thing you have done in 10 min my teacher had taken a week to explain same thankyou soo much mam🙏🙏

always best that are easy to understand science.

@BaaYo

4 жыл бұрын

Thank you Ashif 😊

Umeeer bar jeo

😍😍😍well done

Best explanation

thanks a lot mam

Mam blue white screening and x-gal dye for selection of Recombinant population..is same ???

Where can we get the possible notes for this particular topic?

Mam gene of interest hmesa lacz ke bich me hi insert hota h kya ? Ampicillin ya tetracycline ke bich ni hota h..

Those cells which are non transformants will also give white colonies bcz they dont have lac z So how do we distinguish non transformants from transformants who have undergone recombination

Thanku mam ❤️❤️

Thanks ma'am

happy teachers day mam

Thanks

Thankuuuuu sooo much mam,❤️❤️

Thank you ma'am

Can u make video about corona virus or may be how any virus propagates ,cycles and virulity factors

Thank you Didi

Mam apne lacz gene ka explain kiya and then blue white screening ko differetriate kiya tu kya hum ampicillin or tetracycline gene ke base per blue white screening ko differetriate kar sakte h kya?

mam pls make video on DNA sequencing 🙏🙏

Thanks mam

blue colour colony includes transformed ( self ligated in which gene of interest not inserted, i.e; non recombinants) and non transformed ,how we will distinguish transformed from non transformed in blue colony

@gururajkalshetti198

3 жыл бұрын

No... Blue colour colony only include non transformed i.e self ligated bacterias.

@Quolon

3 ай бұрын

​@@gururajkalshetti198 Transformation is the uptake of DNA by bacteria. If you're saying its non transformed, that means bacteria didn't uptake that plasmid vector, then from where did the blue colour came??

@araw2404

3 ай бұрын

Transformed and non transformed selected on the basis of selectable marker ( vector)and antibiotics ( culture media) only transformed colony are grown on culture media

Thanku mam

Thanks a lot Dear Madamji

@BaaYo

4 жыл бұрын

Thanks Jaydip 😊

did not get link in desceiption box

Mam apka board & marker method best tha with compair to it

Mam aapke telegram gp ka link open hi nhi ho rha plz help

Mam non transformed wali b to white honi chiye????

Mam tele gram link kha diyagya h?

Ma'am a transformed host (recombinant) and a non transformed cell both dont produce blue colour means white colour is retained as usual Now how can we select the recombinant from non transformant Plz reply ma'am

madam bahat achha laga.madam ap kon state me rahate haee??

How to join your telegram group. Link is not given

But mam Non Transformed or transformed with no gene of interest same color denge???then dono ko kse separate kre plzzz bata dee

Telegram ki link open nai ho rahi hai ma'm

Hello mam, here how we will differentiate between recombinant and non transformed cells. Self ligated cells will appear blue in colour but what about non transformed cells how it will be differentiated from recombinant cell. Please clearify

@Quolon

3 ай бұрын

White colour colonies because Lac z gene contains Cloning sites where our Gene of interest gets inserted resulting into disruption of Lac z gene. So now if lac z gene is disrupted, there will be no beta galactosidase production, and thus no conversion of X gal, and thus white colonies.

mam can u please teach genetic code from molecular biology

Can anyone tell Can distinguish non transformed from recombinant transformed cells. As both will give white colony?

@Quolon

3 ай бұрын

This video is confusing. Transformation is necessary in every aspect. If there's no transformation we won't even get Blue colonies. Then comes Recombination, if your Plasmid Vector contains your gene of interest in the Lac z sequence, beta galactosidase won't be produced as the Lac z gene is disrupted by your Gene of interest thus you'll get white colonies. If your vector doesn't contain your gene of interest, that means the Lac z gene is intact which will produce beta galactosidase giving blue colour colonies. Recombinant- White colour colonies. Non recombinant- Blue colour colonies. And non transformants means bacteria didn't uptake your plasmid vector.

The bacrerial cells without any kind of uptake of vector(totaly non transformed) will also produce white colonies, so how to discriminate??

@ranajitpal34

4 жыл бұрын

Exactly, this point is doubt to me.

@PARDEEPKUMAR-pn6eu

4 жыл бұрын

Same doubt

@nidhisingh744

3 жыл бұрын

Watch her replicating plating method. Your doubt will be cleared.

@jaykishansongara7955

3 жыл бұрын

@@nidhisingh744 Ok But I alao found some answers, as I am working in the same area, You should have a double selection marker i.e Antibiotics resistance gene as well as LacZ gene

@nidhisingh744

3 жыл бұрын

@@jaykishansongara7955 you are right. That's only been told in that replica plating video.

Good afternoon mam

Thank you mam. Can you explain the role of polylinkers present in plasmid in gene cloning and also my request you to make a video on the cloning and expression vectors and their features with proper description.

@krutikakatke1270

3 жыл бұрын

What is principal

by blue white screening method

blue product=Bac. Cell - transformation but non recombinant

esy way

Teaching is good ...but so many spelling mistakes ...

Maidam 16 ko genetic engeneering ka exam hai

@BaaYo

Жыл бұрын

Best Wishes

@HBN69

Жыл бұрын

@@BaaYo Thankyou Mam Blotting techniques to aapse hi pdha Marte dum tk nie bhuluga ab

Low standard type teaching

Thanks mam

@BaaYo

4 жыл бұрын

Thanks Arjun 😊