mam thank you so much. I have been watching your lectures since past month and it cleared my concepts. Lots of love from Pakistan. Again Thank You So Much

Nice video with awesome explanation. It's very clear and easy to understand.

I am honestly unable to understand this topic. After trying so much to understand, I came upto utube, nd surprisingly, just in a single time, i am able to understand this topic. Thankyou so much. Please do make videos for class 12 biotechnology subject

Thank you so much mam, you give me a clear concept about replica plating method.

Very nice lectures mam.I am a high school teacher.I leave csir net exam for five years.But Your nice lecture again give me a hope..........I watch your vedeo and again start my preparations......

No one can teach like u mam thanks

Thank you so much mam.. i didn't get this topuc from my zoology teacher .. i was having doubt about the recombinant and transformed cells .. you cleared it so well .. may you live long Thank you once again .. niw i am relaxed since i got the concept ..

@nilanjanbiswas9017

2 жыл бұрын

Thank you mam

Superb explanation...👌👌thank u mam..😍😍

Mam u are tabahii ..ap bhut jyada accha samjhte ho

Your literally amazing.........

Thankyou soo mich mam for crystal clear explain 😊❤❤

thank you mam it helped me a lot.I am a NEET aspirant

Ossmmmmm mam really well explained

Very great explanation of every vedio mam. You are best teacher

@BaaYo

4 жыл бұрын

Thanks a lot

Thankyou so much for the vedio can't thank u enough 🙏🙏

Excellent explanation mam👌👌

Great explanation

Mam please make a video on melting point apparatus ND ph meter

Wonderful mam

So well thaught

Mam ur explaining so nicely

@BaaYo

4 жыл бұрын

Thanks a lot

U r d best guider...mam

@BaaYo

4 жыл бұрын

Thank you Debaswapna...Debaswapna please share this video with your friends and classmates..

@debaswapnaray3295

4 жыл бұрын

Sure mam....☺

Thank you thnku thnkuuuuuu🙏🙏🙏🙏🙏

Grt video again

@BaaYo

4 жыл бұрын

Thank you..

Thank you mam 👍

Thank you Mam

Any technic to use in place of replica plateing to determine the recombinant gene...???

Thank u ma'am

Please make a detailed video on type pf DNA sequencing and Molecular markers....

@BaaYo

4 жыл бұрын

really soon..may be tomorrow...next topic is Molecular markers

perfect but got one confusion. How do we get the ampR colonies back that contain our gene of interest after plating them on amp medium. I suppose they dies off

@shilvanmishra5021

3 жыл бұрын

No they don't die we have to preserve them because we already know that they contain both self ligate and rDNA and the amp plate is called master plate and this process is also known as negative selection because genes which are not of our use are selected and then compared with the master plate to get the host containing rDNA

@durga9776

2 жыл бұрын

see when we are transferring the cells from master plate to the velvet, only some of the cells are getting transfered from each spot. So the master cell still have many cells on each spot. (since the cell size is small each spot contains lot of cells, which is not possible to show pictorially so you may get confuse).

Ma'am please make video on Colony and plaque hybridisation screening methods.

Thanks mam

Mam plz make a video on Gene delivery methods.

Mam very clearly explained But I have one doubt At the last after finding r-dna from non r-dna but r-dna will die due to the absence of antibiotic resistance gene Then how can we get our r-dna back?how these method is useful then?

thank you mam

thanks madam excellent

@BaaYo

4 жыл бұрын

Thank you Mahpara

Ma'am can u plzzz give chapterwise advice of chapters of csir net n how we can make best use of this quarantine

@BaaYo

4 жыл бұрын

Sunanda jo bhi aapko lag rha hai ki important topic hai aur abhi tak cover nhi hua..is quartine time me us topic ko cover kr lo

Isme se which one is positive selection and which one is negative selection? please bataye mam

maam please provide video on colony hybridization and plaque hybridization and Immunological screening

Can we see these colonies with naked eye or use microscope??? Please help me.

Nice

@BaaYo

4 жыл бұрын

Thanks

Mam if the recombinant don't survive in tetra cycline plate, they how could we get our gene of interest

@wwayimran6911

4 жыл бұрын

In master plate, there are few bacteria reamains.Because we slightly press block on master plate. Then we compare both plates and find which colony took rcDNA.Finally collect those few bacteria remains in master plate.

Mam can you take online live lecture on Technics plz

@BaaYo

4 жыл бұрын

sure Kaveri..

Mum please explain the diagram for visually impaired students

What should I do after bsc biotechnology

@BaaYo

4 жыл бұрын

depends on your interest

@saivsk1827

4 жыл бұрын

Can you suggest me which is the best way

Mam is technique main Tet (r) colony ko jab amphiceline replica per le jate hain ye colony to dead ho chuki hain... iska fayda kya hua????

@yrbigfan

3 жыл бұрын

but we have already plated simulatneoulsy plated them on thw woden block so by doing that the colnies will be in parallel and after the tetracycline is added to peterdush and all recominants die we can compare the petre dish and wodden block and pick out the colonies hope it helped (i also am a student so there might be a misktake)

@FarmanKhan-mi2ji

3 жыл бұрын

@@yrbigfan We see these colonies with naked eye or use miroscope?

@yrbigfan

3 жыл бұрын

@@FarmanKhan-mi2ji most probably under microscope causr their is no cromogenic substrate present

bahat achha madam.ap kon state me rahate haee?😊🙏

@BaaYo

4 жыл бұрын

Karnataka

@treeytbot

4 жыл бұрын

@@BaaYo ok madam answer deneke lia

@treeytbot

4 жыл бұрын

@@BaaYo madam ap kon subject me honours and msc kia haee? mae west bengal state se,PURBA MEDINIPUR district se bolta huu. mae VIDYASAGAR UNIVERSITY k under me BAJKUL COLLEGE me BOTANY honours me study karta huu. 3rd year me,semester system mee.lockdown k karan college off madam apka video maa bahat follow karta huu.mere syllabus me isbar PLANT BIOTECHNOLOGY haee,to mera concept me thora problem tha.apka video dakh kar maee bahat khuch sikha. ak problem hae hamare pas ki,iha thikrup se GUIDENESS nahil milta. in future maee BIOTECHNOLOGY me msc karna chahata huu.madam kuch good ebtrance book suggest kar dijia na please🙏🙏🙏.

@treeytbot

4 жыл бұрын

apka answer ke lia maee wait karta huu. madam ap achhe rahia.😊😊😊😊

@BaaYo

4 жыл бұрын

I am masters in biotechnology

Sabka jawab dia hai apne

Next video kab upload hogi

very difficult to understand

Mam aap ka no. Chahiye kaise milega aap se kuch jaankari leni hai

Plz reply my comments

Indians Indians Indians , when are you guys going to stop posting videos with an English title while the context is explained in what ever the language you speak????????????????????????????????????????

Thank you mam

@BaaYo

4 жыл бұрын

Thanks Kaveri