Alpha Complementation - CSIR NET Life sciences | GATE exam
Alpha-complementation is the most common form of insertional inactivation. In alpha-complementation, the vector molecule contains the regulatory and coding regions for the first 146 amino acids of the ß-galactosidase (lacZ) gene.
E. coli strain in which only the region of the lacZ gene coding for the carboxy-terminal portion of ß-galactosidase is present. In cells that contain a vector without an insert, the amino-terminal ß-galactosidase subunit will interact with the carboxy-terminal ß-galactosidase subunit to form an active ß-galactosidase enzyme (the inducer IPTG is often necessary for transcription). ß-galactosidase catalyzes the transformation of the clear substrate X-GAL into a blue precipitate, giving colonies with an active ß-galactosidase protein a distinctive blue color. In contrast, clones containing a polycloning site into which a piece of exogenous DNA has been inserted (i.e., recombinant clones) will not produce a functional amino-terminal subunit, and will consequently appear white in color. It is the recombinant clones that are of interest, and hence they are preferentially selected from agar plates for further analysis.
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Thank you ma'am, very helpful during quick revision before exams. 🙇🏻♂️🙇🏻♂️
Finally its clear so smoothly get rid of confusion thanks
Today, I have completed knowledge about it after watching this video.......thanks
Awsm mainu v aj mili knowledge Raab tuhadi lbhi umar kre te hmesha khush rakhe tuhanu mam 🙂
Now I know what alpha complementation is.. thanks
Awesome mam,, plz make videos on fundamental processes
Very well explained video thank you Ma'am
Thanks a lot, Madame.
Ma'am jaha vector nahi gaya hai that means alpha part nahi banega... sirf omega banega. Enzyme not working to yaha par x gal conversion nahi hoga giving white colonies right? Apne bola jisme vector nahi gaya wo b blue denge. Pls thoda doubt clear kijiye. I think blue white screening se pehle hi hum non transformed ko (jisme vector nahi gaya) antibiotics ( selection marker) se mar dalte hai. Ye maine apke lecture se hi padha hai purane wale. 😊
Awesome lecture.
Hats off mam...mam please make video on DNA and RNA extraction pleasssssssssee
Thank you so so so much
Thanks a lot ma'am
Vedio was nice but can I ask one question if we have a plasmid with lac z gene that produce tetramer a wild type lac z gene and a bacterial cell which have deleted lac z region that mean it do not produce beta galactosidase at all will it work
Thank you ma'am 😊
Thank you ma'am
Thanks a lot mam
Intermolecular forces par video banaye please
Thanks for such an amazing explain ma'am. Can u pls tell me how to acess ur telegram channel?
Madam, if the plasmid ( non-transformed) one are inserted in bacteria (which don't have lac z Gene), then it can produce a blue product, if it can then it should produce both alpha and Omega
@sehrishkhalid126
2 жыл бұрын
We use strain of bacteria for these experiments, not the actual bacteria.. So it will not convert lactose into glucose or galactose
Nice explain mam
well explained
Nicce
Thanku mam.. 😘😘😘😘😘
Mam why self ligated plasmid don’t form omega subunit… if it is altered plasmid then how it is beneficial… ?? Anyone
Nice
Thanks mam
Gabaj mem .... Tqqq
Ma'am why in ncert it's given as alpha insertional inactivation??wts diff bet them??pls ans tommo is my board🥺🥺🥺🥺🥺🙏🙏🙏🙏🙏don't ignore maam
Mam please inorganic metal clusters ke bareme explain ek video banayiye please mam this qurantine days me bahut use hogi I am from Hyderabad
I need the notes but telegram link is not opening
Thank you mam