Thank you, sir, you showed them really easy and simple. I have a blood bank class and tomorrow is our lab test on these things. Thank you

Best one I've seen so far. Thanks for showing the tube labeling and grading of agglutinations. We were all confused the first time. So this really helps. I can't thank you enough. ;)

@robertbounds8112

8 жыл бұрын

+Grace Smith you are very welcome! Thanks for watching! Let me know if there is any other videos you'd like to see.

Thank you. This video explained a concept I was having a hard time with.

Thank you.. New follower From Saudi Arabia..😇

That was the suspension preparation of a man who has done this far too many times to count.

@ovidyuad6687

15 күн бұрын

T

Great, Sir can u please make a video regarding making of 5% red cell suspension

Nice channel, thank you

If i want do antibody screening control gel card? Can u tell me how?

How many drops of RBCs do u pipet into the 3% tube

How long should you spin in the centrifuge ? Thank you..

What is the purpose of “Reagent red cells A1 and B”?

Hi Robert! Thank you so much for this useful video. I have a (perhaps silly) question. Why can't the reverse reaction be done with whole blood, not just serum? Is it that that the antibodies aren't concentrated enough in whole blood and the reaction wouldn't be very visible?

@robertbounds8112

2 жыл бұрын

I suppose it would still produce an agglutination but it would be mixed field due to the presence of the patients cells which could be problematic. A forward type alone would be okay with whole blood (if you lacked the ability to centrifuge). However, if a cold agglutinin was present your forward type could be erroneous. It’s important to perform a negative control (e.g. saline and patient cells) when the entire forward type is positive (I.e. AB positive patient) to avoid such a mistype.

@ljiljanadukanovic6848

2 жыл бұрын

@@robertbounds8112 thanks a lot!

I love this lab, really fun. It was too bad we didn't use our own blood to do the lab ...

Thanks alot my lecture. Could you make video about blood glucose estimation

good job thank you

@robertbounds8112

3 жыл бұрын

Thank you and you’re welcome. 😀

So what is the result Sir?

Thank you! :)

@robertbounds8112

8 жыл бұрын

You're welcome!

I would love to see you remake this video by putting all the clear stuff in first, including the patient's plasma in reverse tubes, then add the patient and reagent red cells so that way the students won't forget to add it. Hard to tell once covered by red cells, trying to avoid ABO discrepancy

@robertbounds8112

2 жыл бұрын

I suppose I do it that as a matter of habit. I usually set up a tube ABORh and gel card with all reagents added (I do this whilst the pink top is spinning). Then I add patient plasma to the gel card and reverse type followed by making a 3% cell suspension for the forward type. I don’t separate the plasma before hand so it flows best this way.

I have 15 years blood banking experience would love to come work in the USA.❤❤❤

In forward typing the serum of recipient is used? How about in reverse typing what sample is needed?

@claumonty

9 ай бұрын

forward sample: red cells reverse sample: plasma

Great video thnx so much. Can u pllzzz make a video on how u can work out which group it is. My teacher explained it i didn't get her. She said something abt 'the 3 rules control, oppositr reaction strong reaction' i didn't get this plz explain

@robertbounds8112

8 жыл бұрын

+Jessie Molly HI, I added some videos that might help you. Was your teacher referring to antibody rule outs using 3 cells and homozygous and heterozygous cells showing dosage (weaker or stronger agglutinations)?

@empathlove5576

2 жыл бұрын

@@robertbounds8112 I think she meant the different blood types. Each shown in a aborh A+, B- etc.

@adarshrana4189

8 ай бұрын

O+

thanks

@robertbounds8112

8 жыл бұрын

+anmar ali You are very welcome. Thanks for watching!

So the blood type was O+?

@tas9019

5 жыл бұрын

Yeah

@Jeagerjaques

4 жыл бұрын

I like this lab, so much fun

you should use serum instead !?

@robertbounds8112

Жыл бұрын

Serum could be used but EDTA plasma is more common where I have worked. We do use serum for neonates.

2:00 why two drops?

@emmaz.7397

3 жыл бұрын

He might have wanted a stronger reaction. There are situations where you may need to increase the plasma:cell ratio

how to prepare 3% washed Rbcs

@gurisaini348

4 жыл бұрын

moody moody you must add 30 micro litre of rbc in 970 micro litre of saline to make 3% suspension Or you can add 15 micro litre of rbc in 485 microlitre of saline .

0:25 Are you sure is that plasma ?

@robertbounds8112

7 жыл бұрын

It's plasma. Although you could use serum too.

@ScrooobTV

7 жыл бұрын

Oh ok. Because I've never seen a plasma used for reverse ABO test.

Where is anti AB?

@robertbounds8112

8 жыл бұрын

At my facility we only use anti AB when processing Type O units into our supply (from One Blood and such). We do not use it for our forward and reverse typing.

ممكن بالعربي

@ademes889

6 жыл бұрын

I'll translate for you

can you determine the Rh with reverse typing?

@robertbounds8112

7 жыл бұрын

You cannot. Only ABO type.

@AbdulQuddus261

6 жыл бұрын

no

Told in hindi

What this ? So thanks u make it so difficult to me I’m really confused 😐 If the agglutination like this 🔴 consider as positive ? If after mixing disappear is consider as negative or what !!! 🥲🥲 I know it’s easy but WHY is confused me 😭