Authors: Konrad Suesser, James Choi (MEDS 490 Directed Studies)
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Пікірлер: 21
@sachin27109314 күн бұрын
Very nice Thank you
@sweetandsexy2202 жыл бұрын
Very thorough! Including technical descriptions as well as conceptual. Thank you for this!
@user-ql7vq6xk2v9 ай бұрын
I can't thank you enough for such detailed information.
@meahahmed3709 Жыл бұрын
This is an absolutely fantastic video thank you so much.
@loktiz2 жыл бұрын
Great content! Thanks for this!
@yurobert30078 ай бұрын
This is such a sophisticated experimental procedure. Thanks for this video.
@naveen99ification2 жыл бұрын
Excellent Content ! Very Informative !
@gabrieldias9512 жыл бұрын
This is great! Thank you!
@XiaochenNiu7 ай бұрын
Thank you for your detailed explanation.
@dante_e.i7468 Жыл бұрын
excellent video. good job man
@perminderghotra2 жыл бұрын
great content
@saurabhgagneja13762 жыл бұрын
Nice content, Thanks.
@soniadisalvo1742 ай бұрын
Thanks for this video, so interesting
@yojetsharma75492 жыл бұрын
Great video and very informative! Is the rest of the video online too?
@parimehr88 Жыл бұрын
You explained how to giga seal in details and perfectly,
@haiquoc2013 Жыл бұрын
What book teaches how to read voltage clamp recordings ?!
@josevicentenegretediaz1703 Жыл бұрын
Hello, where do you place your carbogen gas tank? At my university they ask me for evidence to allow me to place it next to the recording setup. Where is your lab located?
@brianchristie2941
9 ай бұрын
We have portable attachments that allow us to connect it to the bench top securely.
@PhunkyPharmacologist3 ай бұрын
Where can I find more information on the theory and principles behind whole cell patch clamp recording? I'm recording from cultured mammalian cells transfected with recombinant receptors, and I would like to familiarize myself more with the different types of errors (e.g. voltage errors from currents that are too large), how to judge the quality of a seal based on the shape of the test pulse response, etc. as well as how the recording equipment operates (digitization, sampling rate, filtering, etc.) that applies to patch clamp recording in general.
@nik09865
19 күн бұрын
I would strongly recommend giving the Axon Guide a read! You can download the PDF for free on the Molecular Devices website. One major voltage error to look out for is series resistance. Bill Connelly has a blog post titled "Series Resistance. Why it's bad." that explains how it affects your recordings, and a follow-up on how to properly compensate for that. To add, if you plan on compensating for series resistance, the quickest method of minimizing pipette capacitance that the lab I'm in uses is wrapping a thin strip of parafilm around the pipette until you get close to the tip and minimize the amount of internal solution. Another source of error is the liquid junction potential which is voltage offset caused by differences in cation and anion sizes in the pipette solution, which is really only notable if you use a large anion base for your solution like K-gluconate. Nat Blair has a blog titled "What is a junction potential?" and a follow-up titled "How do I measure the liquid junction potential?" that goes over it in better (and more correct) detail than I did. Sorry for not adding links, I'm not sure what KZread's policy is for adding links on comments. I know I'm a little late on the reply, but I hope this helps! Edit: I'm not associated with this lab, but I primarily do patch for my research
Пікірлер: 21
Very nice Thank you
Very thorough! Including technical descriptions as well as conceptual. Thank you for this!
I can't thank you enough for such detailed information.
This is an absolutely fantastic video thank you so much.
Great content! Thanks for this!
This is such a sophisticated experimental procedure. Thanks for this video.
Excellent Content ! Very Informative !
This is great! Thank you!
Thank you for your detailed explanation.
excellent video. good job man
great content
Nice content, Thanks.
Thanks for this video, so interesting
Great video and very informative! Is the rest of the video online too?
You explained how to giga seal in details and perfectly,
What book teaches how to read voltage clamp recordings ?!
Hello, where do you place your carbogen gas tank? At my university they ask me for evidence to allow me to place it next to the recording setup. Where is your lab located?
@brianchristie2941
9 ай бұрын
We have portable attachments that allow us to connect it to the bench top securely.
Where can I find more information on the theory and principles behind whole cell patch clamp recording? I'm recording from cultured mammalian cells transfected with recombinant receptors, and I would like to familiarize myself more with the different types of errors (e.g. voltage errors from currents that are too large), how to judge the quality of a seal based on the shape of the test pulse response, etc. as well as how the recording equipment operates (digitization, sampling rate, filtering, etc.) that applies to patch clamp recording in general.
@nik09865
19 күн бұрын
I would strongly recommend giving the Axon Guide a read! You can download the PDF for free on the Molecular Devices website. One major voltage error to look out for is series resistance. Bill Connelly has a blog post titled "Series Resistance. Why it's bad." that explains how it affects your recordings, and a follow-up on how to properly compensate for that. To add, if you plan on compensating for series resistance, the quickest method of minimizing pipette capacitance that the lab I'm in uses is wrapping a thin strip of parafilm around the pipette until you get close to the tip and minimize the amount of internal solution. Another source of error is the liquid junction potential which is voltage offset caused by differences in cation and anion sizes in the pipette solution, which is really only notable if you use a large anion base for your solution like K-gluconate. Nat Blair has a blog titled "What is a junction potential?" and a follow-up titled "How do I measure the liquid junction potential?" that goes over it in better (and more correct) detail than I did. Sorry for not adding links, I'm not sure what KZread's policy is for adding links on comments. I know I'm a little late on the reply, but I hope this helps! Edit: I'm not associated with this lab, but I primarily do patch for my research
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