I watched this kind of content as a student. Then I watched it as a PhD student, when I worked with this technique. Now, at a Post-Doc level, coming back to the setup...A screw it, let's watch that; I might actually learn something! EDIT: Holy crap...ACTUALLY learned something! Well done, Professor! Thanks a lot!

@pepaxxxsvinka3379

22 күн бұрын

I am doing PhD now too!

This was really clear and easy to understand, and somehow much better than the one and a half hour lecture I got on fluorescence confocal microscopy yesterday. Thank you. I'd also really like a video on atomic force microscopy as well as on other more recent types of microscopy like the 3D LIMON microscopy you showed here.

As always, thanks for saving the day Dave.

I did single molecule fluorescent imaging for my doctorate. Great to see the field promoted 😃👍🏻

@ameliac504

Жыл бұрын

oooh

wow, never woulda thought a random question on my mind was answered by such a boss. so much succulent knowledge here. lit

I've been using confocal in the lab as an undergraduate for 3 years and I feel like I finally understand, thank you!!

@davyc412

2 жыл бұрын

Nothing like taking a thick stack only to find a massive black square on your embryo when you're ready to shoot your overview. Moral of the story, take your overviews first.

This was quite helpful, thanks!

This is so helpful. You are awesome!! Thanks a ton!

I'm watching this cuz it's entertaining and helpful!

Brilliant work Sir🥰🥰🥰🥰

Watching this for my exam preparation. Made me understand immunoflourescence and phenotyping in flow cytometry much better - THANK YOU.

Very helpful!

very nice, thank you

Very good!

I love this

This is very cool

I like your funny words magic man

Wow nice sir love from Pakistan

Hi.. sorry, what do you mean with relaxation causes the emission of a photon? one may be confused with this term. relaxation in fluorescence is often understood as vibrational relaxation or non-radiative relaxation. I would say the electron returns to its ground state instead of relaxation to describe fluorescence process, even though one person may choose this term to explain fluorescence.

@ProfessorDaveExplains

2 жыл бұрын

Hmm, I always thought the term applied to any electron moving to a lower energy shell. Does it not? Fluorescence is just a subset of this phenomenon.

@arfianwisnu1253

2 жыл бұрын

@@ProfessorDaveExplains Actually you are correct. Relaxation is only electron movements, from higher energy state to lower ones; either it is non-radiative or radiative. But term of relaxation is mostly understood as vibrational relaxation, which is non-radiative. Some researchers may call the radiative relaxation as transition, although both are the same. For Master student or higher, your term is OK (hopefully they can distinguish), for Bachelor this may distract if you do not explain completely.

Greeting to prof. Dave As you had explained the working /functiong of flouroscene microscopy In which I have a doubt that if there is no energy transition for a perticular part (may be due to the provided energy is less than the required energy OR MAY BE SOME OTHER REASONS) it will not be seen and the structure will be wrong So how one can know whether the final structure , which is seen by help of it, is correct or not ?

@ujala6542

3 жыл бұрын

Or may be the case where the wavelength, Which emits , are not belong to visible light

4:43 Why we need a dichroic mirror? Won't it be as efficient (less distortion and cheaper) to just aim the filtered light directly to the sample, in 45 degree angle for example. Then, the fluorescent light is emitted and filtered. Let me know if I won a Nobel Prize for that. I'll stack it with the other I have in the garage.

Great work professor. these videos help us understand the reality and rejects religious myths

Hey Dave. I’ve been wondering if you have any videos about human psychology or explaining how emotions work. Please tell me if you do or are planning to make any. Thx for the channel.

@prince5063

3 жыл бұрын

Nvm, just found one lol

@ProfessorDaveExplains

3 жыл бұрын

Yep just that one for now but I’m planning a proper psychology series!

@prince5063

3 жыл бұрын

@@ProfessorDaveExplains Thank you, hoping to be a therapist in the future.

Let's hope that we can use proton and neutron energy instead of electron for microscopy

Hey Prof. Dave, could you do a video on why there are firefly chemicals in the new vaccine for covid 19?

@rickkwitkoski1976

3 жыл бұрын

@Matt Parker HEY MATT!!! You are a complete and utter ignorant FOOL!! Are you talking about luciferase and luciferin? Yeah. CHEM ICK ALS!!! EVERY THING is a chemical bud!!! You are BREATHING chemicals right now! And you drink a toxic chemical every single day! Ever hear of dihydrogen monoxide?? What about the deadly chemical Pentahydroxyhexanal??? That is inFUSED in you right now! Luciferase is an enzyme. Enzymes are PROTEINS!!! And luciferin is a compound that has fluorescent properties. These are used in the DEVELOPMENT of many biologically active substances, including VACCINES! An assay method was developed using the reaction of this enzyme and its substrate to help determine the efficacy of biomolecules. They are not PART of the COVID-19 vaccines. JUST LIKE human tissue is NOT in the vaccines. Cell lines derived from aborted fetuses from 50 years ago (amazing that these cell lines are still with us), originally developed to help in CANCER research, are also used to develop various vaccines including the mRNA vaccines of Moderna and Pfizer Biontech. The cell lines are use to proof the efficacy of immune responses in human cells. Pull your dumbass out the conspiracy crap and actually learn some REAL SCIENCE!!! Dave didn't answer you because your piddly ass is not worth his time.

@x00g40

3 жыл бұрын

@@rickkwitkoski1976 hey Rick, take a break buddy

I believe i Will study it in biophysics.

@DrBovdin

3 жыл бұрын

You will be hard pressed avoiding it 😉

@mariocesarsousa

3 жыл бұрын

@@DrBovdin i really cant.....it is part of the curriculum. Unless i had chosen the teaching curriculum. Bacharelado 👈

@DrBovdin

3 жыл бұрын

@@mariocesarsousa that’s what I meant. You can’t avoid an integral part of one of the most powerful and most commonly used analysis tools we have in the field.

@monkeybusiness673

3 жыл бұрын

Look forward to it. It's one of the coolest things I ever encountered in biophysical chemistry!

professor dave, how can they tell there's a coronavirus if it's impossible to tell if rna in a cell culture is of cellular or viral origin? what true scientific control have they to tell the difference? thanks for reading

@ProfessorDaveExplains

3 жыл бұрын

It absolutely is not impossible. We can sequence genomes. We know the organismal identity of any genetic sequence we are working with. This is trivial.

@NathanW5555

3 жыл бұрын

@@ProfessorDaveExplains thanks for taking the time to respond to my question, i do appreciate it, i thought the question of identifying viruses might relate to the above topic, similar to the use of luciferase to assay transcriptional activity of regulated activation sequences of DNA - pubmed.ncbi.nlm.nih.gov/2698191/ (as in the use of the property of fluorescence to give us information on things). i could ask you about 20 questions and i wouldn't want a focus on a trivial topic but i'll ask is there a method to cause bioluminescence of viruses to identify viruses or can fluorescence microscopy identify viruses? what's the control to distinguish which of the rna is cellular or viral? again, thanks very much for your response

@ProfessorDaveExplains

3 жыл бұрын

No problem, happy to help, though of course my utility is limited to very general questions such as your first one. I don't know much about the specifics of laboratory techniques as I'm not a microbiologist. Someone else wrote these microscopy scripts.

@NathanW5555

3 жыл бұрын

@@ProfessorDaveExplains Gentleman, thanks very much for your time and patience.

@marquisecuffe4061

3 жыл бұрын

@@ProfessorDaveExplains professor dave, you should make a video on how race realism conspiracy theories are pseudoscientific. thanks for reading.

because i tried to send you a present but i couldn't bec. i need your mail

e

can i have your mail