Couldnt ask for a more clearer explanation than this, you made my day. Thank you so much!!!

One of the best videos about PSF out there!

Fantastic video. The visuals are very well made! Also appreciated the image of the Abbe memorial. I see it almost every day when I walk to uni. :D

PSF? More like "Perfect videos; you're the best!"

The best explanation on PSF. Completely agree with all the comments below. Thank you very much

One the best lectures of PSF

Rightly said an expert can make it simple!! Muchas gracias!

This is by far one of the bests videos I have ever seen.

Thank you very much for this video! It was very informative and I learnt how the PSF relates to the Rayleigh resolution limit!

Lovely to see the pink heart picture flashing out when doing the axial scanning (around 3'30") and she says "the PSF is beautiful!".

wonderfully explained! Great narrating too!

I seriously applaud these videos. They are so thoughtfully put together and present the information in the clearest way possible. I can only hope that every instructional video out there some day explains things in an equally engaging, informative, and simple format. Well done!

@Microcourses

2 жыл бұрын

Wow, thank you! I'm so glad you find them helpful.

amazing video. I can't wait for future courses

this is an amazing explanation about PSF. Thank you very much!

you were able to make me understand these concepts with a 12 minute video

Best explantion out there. Congratulations.

Very clear explanation, thanks.

Thank you so much for this informative video!

Besides the visual stress felt from staring at the bright circular light shown in the initial half of the video, it was an amazing explanation on point spread function.

Thank you for your great explanation!

Excellent illustrations of PSF

Thank you so much for this video! Really helped me understand PSF without having to read pages and pages of writing!! You deserve more subscribers.

@Microcourses

3 жыл бұрын

Glad it was helpful!

Fantastic.. I just taught a PG class on PSF.. This helped me a lot to prepare materials.

Incredible video! Really helped me out with my microscopy class

@Microcourses

3 жыл бұрын

Great to hear!

Pretty neat explanation! What is missing for a nerd like me are some remarks on the differences between coherent and incoherent imaging and Abbe theory of image formation. What was really enlightening to me was when I read the treatment of Goodman on Fourier Optics that shows that in the coherent case, Abbe theory based on the collection of diffraction orders from the object interfering in the image plane and the convolution approach using the Amplitude PSF are equivalent, because the amplitude transfer function involves a second fourier transform of the Airy Disc PSF that gives a circ while the incoherent case (e.g. from a self-luminous object) yields the autocorrelation of the aperture function because of the square operation in the spatial frequency domain (convolution theorem).

thank you for this amazing content :)

no body can explain like this thank you very much

excellent video, damn!

amazing video

Thank you for the brilliant lecture

@Microcourses

3 жыл бұрын

Glad you liked it!

excellent!

Now I get the whole point of interference and diffraction in microscope!

really useful

Thank you ♡

how does enhanced resolution work (e.g. closing a pinhole a little bit)?

I've never come across lateral and axial in these waves... Thanks...

Hello. I found your video very explanatory, since I'm currently doing a presentation about PSF and diffraction limit for my University. Can I use some of your visuals for the presentation, and if so how can I give reference? PS sorry for poor english

@Microcourses

3 жыл бұрын

Yes, please do! You can reference the channel URL: KZread.com/microcourses.

@AP-vd8ve

3 жыл бұрын

@@Microcourses although I finished the presentation , thank you, for giving me the permission and for helping me understand the terms

Nice video. However, two slits in front of a classical wavefront demonstrating interference is not the double-slit experiment; that refers specifically to the self-interference of a single particle's encountering a double-slit.

Are all fluorescence emissions coherence to make an interference pattern at the image plane?

@Microcourses

4 жыл бұрын

Fluorescence emitted from a diffraction limited spot is partially coherent, and will therefore result in an interference pattern.

So the PSF is essentially the impulse response of the imaging system?

Just a question. What wavelength is being spoken about here? It is the emission wavelength of the green light right?

@Microcourses

3 жыл бұрын

Yes, emission wavelength.

Thanks for your great vedio about PSF,do you have another vedio about OTF?

@Microcourses

4 жыл бұрын

Not yet, but it's on our (long!) to do list.

Well I’ll just watch this 20 more times until my dumb brain catches on.

This is an exceptional video, but the diatom at 1:00 is certainly not Pleurosigma angulatum. Not trying to nit-pick or act smug, this is one of the best PSF videos I have watched so far.

@Microcourses

Жыл бұрын

Thank you! My records may be incorrect. What species is it?

@diatomsaus

Жыл бұрын

@@Microcourses I think it should be Frustulia rhomboides.

By the way, it is one MAXIMUM and two or more MAXIMA ;-) @ 3:53 And this really is 2x the amplitude ;-(

Stop the vocal fry please

@mar2lka

2 жыл бұрын

I am very curious if you ever commented that under a video of a male speaker...