Thank you from Malaysia..your vids are really helpful :-)

Great video. you have a smothing voice, and it is quite pleasant.

ooooh wavo it was amazing demonstration

Thank you very much! I can't wait to test my sample using the instrument.

@chemfunman

2 жыл бұрын

당신은 가장 환영합니다! 진심으로 감사드리며 이 영상을 다른 사람들과 공유하시길 바랍니다. 축복받은 날 앞으로!

Thank you , it is helpful !!

thank u soo much for this great video

This is very helpful Thank you

NEW UPDATED UV Vis video HERE kzread.info/dash/bejne/i4xmpq2rqLGwpqw.html SUBSCRIBE NOW to this channel now to receive more great videos! Connect with me on Twitter @chemfunman (twitter.com/chemfunman) Stay safe! “Ever tried. Ever failed. No Matter. Try again. Fail again. Fail better.” - Samuel Beckett

@nwanduchikeluba4094

3 жыл бұрын

Thank you. How should I go about it using an extracted polysaccharide as sample to test for binded protein?

Informative

Awesome!!

alraight,thank you

Sana all pwede makagamit ng magandang UV vis

good understanding us

Thank you very much sir

This is great!

Very helpful one😊

I appreciated tha thanks Mr

Thank you

love this video..i too using uv vis 1800...this will be very helpful to me..tq very much..

Thank you I understood uv operation

Great😊

@chemfunman

Жыл бұрын

Thank you! Cheers!

Thanx 👍👍👍

@chemfunman

3 жыл бұрын

Welcome 👍

Your channel has been SO helpful to improve my lab skills!! Thank you so much

@chemfunman

2 жыл бұрын

I'm so glad! You are most welcome!

@uhoh5473

5 ай бұрын

@@chemfunman when is says maximum absorption wavelength for compound does that mean if you shine a light at that wavelength it absorbs it the most 8:38

@chemfunman

5 ай бұрын

this is true given the range of wavelength, so from this standpoint, it is right@@uhoh5473

⭐Amazing demonstration video sir!👌 If you don't mind, Can i use your demo video to explain in my native language please with proper credits to you? thank you for this awesome video sir!😊

@chemfunman

4 жыл бұрын

Thanks for your suggestion. Can you share more about how you plan to do this translation? email me at fun.man [at] u.nus.edu

Thank you very much.

@chemfunman

8 жыл бұрын

+Seif Eddine B You are most welcome.

thank you

This video is absolutely full of informations👍👍

@chemfunman

3 жыл бұрын

Merci pour votre réponse !

Thank you very much for your useful explanation

@chemfunman

6 жыл бұрын

Eng. safawy you are most welcome!

감사합니다^^! thanks♥

@chemfunman

7 жыл бұрын

If I am not mistaken, the korean Han-gul is read as kam-sa-ha-me-da? ^^

@pranavkumar09

2 жыл бұрын

@@chemfunman Sir this is read as gamsahamnida which means Thank You ( in formal manner )

It would b a great help if you can explain to interprete the data from calibration curve (especially for the estimation of Ammonia in water). Thank you

@chemfunman

2 жыл бұрын

Let me work on that!

thank you so much for the explanation! 🌹

@chemfunman

2 жыл бұрын

You're so welcome!

Nice one , maybe you can buy one to play in home 🤪

@chemfunman

Жыл бұрын

That’s right!

@tsoekmanyiu8580

Жыл бұрын

I did have one, but not Shimadzu The one I have is ocean insight STS series

@tsoekmanyiu8580

Жыл бұрын

STS series much smaller than Shimadzu uv1800 The size much smaller than EZ link card for MRT

I am using your video for online practical class during this lockdown period. It is very useful. Thanks a lot.

@chemfunman

4 жыл бұрын

You are most welcome. Feel free to use and share. Perhaps the best way is to have students to scaffold their thoughts in this platform. I find that it worked for my students as they spark ideas and clarify concepts

nice video

Thank you so much, the video is quite clear and understandable.

@chemfunman

2 жыл бұрын

You are welcome!

Very Nice demonstration,

@chemfunman

7 жыл бұрын

Thanks for your support! Share the good stuff.

Thank you for your explanation

@chemfunman

8 жыл бұрын

+Alex Tientcheu you are most welcome, take a look at other videos if you find them useful.

Thank you for the explanation . I have now literally understood the process ..... ✌🏼💜

@chemfunman

2 жыл бұрын

You're so welcome!

Excellent video and great explaination

@chemfunman

3 жыл бұрын

Glad you liked it!

spectrophotometry is very important in chemistry

Thank you so much

@chemfunman

2 жыл бұрын

You're most welcome

Thank you so much . This has been very useful for my practical exam tomorrow

@chemfunman

3 жыл бұрын

thank you! you are kind, do share these videos and subscribe.

So helpful, thank you

@chemfunman

3 жыл бұрын

You're so welcome!

Thank you, it is very helpful! :)

@poragpori1226

2 жыл бұрын

Please help me,why we use it in Laboratory?

@poragpori1226

2 жыл бұрын

Actually what do we measure from this machine?

Nice

Hello Dr. Fun, this was a great video! (You should have named your channel "Dr. Fun", it would have been dope!) Requests: Are there any videos which explains the interepretation part of the data generated? I am well versed with the basic principles of spectroscopy, want to see what happens after the results are generated and where is that data stored and used. The data would be realted to the sample concentration, yes, but what next. I prefer the applications of UV/Vis spectroscopy to be of pharmaceutical/biotechnology domain. It would be of great help if anyone can share the links to videos or articles or books. Thank you! Happy learning to all!

@tsoekmanyiu8580

Жыл бұрын

gahan, you can buy one spectrophotometer in home to play with it :p

what reference you use for carbon quantum dots for this study

Awwsomee...very well explained✌👌

@chemfunman

3 жыл бұрын

thank you! you are welcome - come back to this channel for more! like and subscribe :D

nice

Good

@chemfunman

2 жыл бұрын

That's so awesome to hear!! Do share this with others :D

Sir plzz tell me..." For UV/Visible spectrophotometric graph the protein that is taken is first diluted??...mean first we take the stock solution of protein and then diluted it and then placed in spectrophotometer for measuring the absorbance ( for example at 280 nm)...

Thankyou

Tq so much sir

Excellent 👍😊

@chemfunman

2 жыл бұрын

Thank you! Cheers!

Thanks.

@chemfunman

2 жыл бұрын

You're welcome

This is helping, thank you🙏

@chemfunman

3 жыл бұрын

You’re welcome 😊

Very nice understanding concept

@chemfunman

2 жыл бұрын

hope you liked our effort, thanks!

Very helpful thank you 😊

@chemfunman

3 жыл бұрын

Glad it was helpful!

Outstanding

@chemfunman

Жыл бұрын

It's my pleasure!

👍🏻

@chemfunman

2 жыл бұрын

(Y)

Very helpful vdo, thanks

@chemfunman

3 жыл бұрын

Glad it was helpful!

❤️❤️❤️

Very nice explanation thanks for that

@chemfunman

2 жыл бұрын

Glad it was helpful!

great working

@chemfunman

Жыл бұрын

Nooice

Thank you very much

@chemfunman

4 жыл бұрын

You are welcome

Thanks

@chemfunman

3 жыл бұрын

No problem

Спасибо!

@chemfunman

2 жыл бұрын

welcome!

Thanks ...It really helped me..I learned from this video how to operate it...I studied theoretically ...So this practical demonstration was helped me a lot..Thank you so much

@chemfunman

6 жыл бұрын

most welcome!

Thanks man

@chemfunman

3 жыл бұрын

You're welcome!

Thank you :)

@chemfunman

3 жыл бұрын

thank you so much! do Subscribe and share this with your friends! :D

Tkank you fot this test

@chemfunman

2 жыл бұрын

thank you for your support! merci! do subscribe and share our videos with your friends :-)

Thank you soo much really helped me

@chemfunman

2 жыл бұрын

You're welcome!

good

@chemfunman

2 жыл бұрын

Super

Nice sir please make learing video about LCMS Cromategraphy

thank you so much , this has been very useful for my pratical exam ...

@chemfunman

2 жыл бұрын

You are most welcome

Thank you so much ❤️

@chemfunman

2 жыл бұрын

You’re welcome 😊

Sir you expline tha animation are also very helpful

Thank you for making this video. And, I have one question, Is it use the same with uv-3000 spectrophotometer?

@chemfunman

4 жыл бұрын

very much the same!

@povraksmeyphuong

4 жыл бұрын

@@chemfunman Thanks U teacher

Very nice.

@chemfunman

3 жыл бұрын

Thank you! Cheers!

Amazing

@chemfunman

3 жыл бұрын

Thank you! Cheers!

Thank u so much

@chemfunman

3 жыл бұрын

Most welcome 😊

Very good demonstration. I guess different cuvettes are needed for UV and visible light measurements

@chemfunman

2 жыл бұрын

Yes, definitely!

hi i use this technique and measured the absorbance of my sample but i am getting 4.00 at 303.5,272.5 and 228nm...this sample is analysed using hplc at 280nm...the max abs is 4.0...do i need to dilute spl and scan again?

@chemfunman

5 жыл бұрын

I don't think this is needed.

i have any question, what are different between autozero and basecorection? we usualy using basecorection for spectrum, autozero for photometric.

@chemfunman

11 ай бұрын

If this situation arises , it can be corrected by performing an autozero operation (press the “Autozero” button.

hi, what will I do if I have blank (all reagents + water instead of sample ) and another blank (full of water) . I ll read against water. Im confused about two different blank solution :( Could you please explain ?

👍👍👍

@chemfunman

2 жыл бұрын

Awesome, keep watching, and sharing!

thank for your lecture. could you help me to combine the datas together on one graph? thank you!!!

we want such kind of spectrophotometers in our university

@chemfunman

2 жыл бұрын

yay

Finally a non-Hindi accent video :D (Thank God), Very good explanation, very practical, to the point and easy to understand. THANK YOU!!!

@chemfunman

4 жыл бұрын

YOU ARE MOST WELCOME!! thanks for your support.

Hi . I am telling from Bangladesh.I read in class 12 in St.joseph higher secondary school. You are very helpful

@chemfunman

3 жыл бұрын

thanks!

Good very good

@chemfunman

4 жыл бұрын

Thanks

should we get the curve calibration and lambda maximum first before testing the sampel? or does it not apply to double beam spectrophotometer?

@chemfunman

Ай бұрын

Yes you are right

@rianaaja144

Ай бұрын

@@chemfunman thanks for answering, didn't expect it because this video is 7+ years ago😤

@chemfunman

Ай бұрын

@@rianaaja144 still valid today yea?

Very good

@chemfunman

4 жыл бұрын

thanks!!

Hi Dr, What is the difference between baseline and autozero. When you want to determine lambda max of a solution (unknown), as the first step keeping only solvent in both of the slots should we go for autozero or baseline ? Thanks in advance!

@chemfunman

2 жыл бұрын

Do autozero after you have set the baseline. The baseline is set when you have a standard you wish to use as a reference value, say, a solvent. Adding a solute incurs matrix effect.

@prefer_not_to_say

2 жыл бұрын

@@chemfunman It worked. Thanks a ton Dr Fun Man Fung.!

If we want to take reading at different temperatures, is it possible with UV-Vis 800?

@chemfunman

Жыл бұрын

Looks likely

Please tell us how to interpret the data from the calibration curve in oder to estimate a particular compound in water sample like benzene or Toluene

thx

@chemfunman

8 жыл бұрын

+little k most welcome!

What happen if I missed to autozero? Is my data obtained without autozero before analysis still acceptable?

@chemfunman

2 жыл бұрын

Yes

very good and explanation and easy understand. I would like to ask if I use distilled water for soaking material purpose. is it possible to use such distilled water as my blank solution?

@chemfunman

8 жыл бұрын

+Cheng ManXin Yes you can.

Love you sir

@chemfunman

2 жыл бұрын

Awesome, keep watching, and sharing!